• The Korean Journal of Zoology
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• v.8 no.1
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• pp.11-14
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• 1965

The object to this study is to identify the genus and species of the microfilariae which were recently found in the area of Youngju-Gun. THe identification of the microfilariae was made on the morphologicla aspects. 1. Blood samples were collected through vena punction from known microfilariae carriers living in the newly confirmed filaria endemic area of Youngju0Gun in Jyongsang Pukdo Province. Youngju-Gun is located in the mountainous central part of Korean Peninsula. 2. The following fixation and staining techniques were applied. (1) Fixation by drying in the air, followed by staining with Azeo or Giemsa. (2) Knott's fixation method (2% formalin), followed by staining with Azur II. 3. A comparative study of the body length of the microfilariae after different fixation and staining techniques were applied. (1) Knott's fixation method followed by staining with Azur II : average body length found was 28.4$\mu$. (2) Dry fixation followed by staining with Giemsa : average body length found was 209.4$\mu$. (3) Dry fixation followed by staining with Azeo : average body length found was 205.4$\mu$. 4. The locations of the different body cells were measured in 60 individuals of microfilariae in the wet preparation fixed by Knott's method and stained with Azur II. The distance of the different body cells to cephalic apex of microfilariae was measured and calculated as a percentage of the total body length. The average results are as follows : BNC , 3.04% ; N, 22.74%, EP, 31.4% ; EC, 37.77%, G1 cell , 67.94%; G2 cell, 73.54% : G3 cell , 75.55% ; G4 cell, 77.65% ; AP, 82.02%%. 5. As a result of the above findings the microfilariae found in the above mentioned area could be identified as Brugia malai(BRUG, 1927) BUCKLEY, 1960.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.9_10 s.146
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• pp.1274-1284
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• 2005

Effect of fixation methods and relaxation treatment on the dimensional stability and physical properties of MDPPA/HMM treated cotton knitted fabrics were studied. Combination of four different fixation methods - relaxation, swelling agent treatment, pad dry cure fixation, and wet fixation - were applied to flame retardant finish of 4 kinds of cotton knitted fabric with MDPPA/HMM. Then these fabrics were washed 10 times. As a result, In swelling treatment on 10G showed relatively higher value of length shrinkage than 14G. Length and width shrinkage were increased by initial washing treatment and no further change was shown after 6 washing cycles. After 10 washing cycles, length and width shrinkage decreased. The KES standardized basic value of B/W, 2HB/W and bursting strength of interlock were relatively larger than those of single jersey. The values of B/W and 2HB/W of cotton knitted fabrics were increased by relaxation and washing treatment but were decreased by swelling treatment. In addition, the bursting strength of the cotton knitted fabrics was decreased after fusing, washing and relaxation treatment.

• Archives of Plastic Surgery
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• v.40 no.3
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• pp.259-262
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• 2013

After skin grafting, to prevent hematoma or seroma collection at the graft site, a tie-over dressing has been commonly used. However, although the conventional tie-over dressing by suture is a useful method for securing a graft site, refixation is difficult when repeated tie-over dressing is needed. Therefore, we recommend a redoable tie-over dressing technique with multiple loops threads and connecting silk threads. After the raw surface of each of our cases was covered with a skin graft, multiple loop silk thread attached with nylon at the skin graft margin. We applied the ointment gauze and wet cotton/fluffy gauze over the skin graft, then fixed the dressing by connecting cross-counter multiple loop thread with connecting silk threads. When we opened the tie-over dressing by cutting the connecting silk threads, we repeated the tie-over dressing with the same method. The skin graft was taken successfully without hematoma or seroma collection or any other complications. In conclusion, we report a novel tie-over dressing enabling simple fixation of the dressing to maintain proper tension for wounds that require repetitive fixation. Further, with this reliable method, the skin grafts were well taken.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.366-366
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• 2016

Nanoscale observation of attachment systems of animals has revealed various exquisite multiscale architectures for essential functions such as gecko's locomotion, beetles' wing fixation, octopuses' sucking and crawling. In particular, the hierarchical 3-dimensional hexanonal nano-architectures in the tree frog's adhesion is known to have the capability of the enhancement of adhesion forces on the wet or rough surfaces due to the conformal contacts against rough surfaces and water-drainable micro channels. Here, we report that tree frog-inspired patches using unique artificial 3-dimensional hexagonal structures can be exploited to form reversibly enhanced adhesion against various highly curved and rough surfaces in dry and wet condition. To investigate the adhesion effect of micro-channels, we changed the arrangement of microstructure and spacing gaps between micro-channels. In addition, we introduced the 3-dimensional hexagonal hierarchical architectures to artificial patches to enhance to conformal contacts on the various rough surfaces such as skin and organs. Using the robust adhesion properties, we demonstrated the self-drainable and comfortable skin-attachable devices which can measure EKG (electrokardiogramme) for in-vitro diagnostics. As a result, bio-inspired programmable nano-architectures can be applied in versatile devices such as, medical patches, skin-attachable electronics etc., which would shed light on future smart, directional and reversible adhesion systems.

• Applied Microscopy
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• v.52
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• pp.4.1-4.11
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• 2022

Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the "nanosuit" later referred to as "NanoSuit", describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy.

• Korean Journal of Environmental Agriculture
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• v.24 no.3
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• pp.295-302
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• 2005

The nitrogen (N) balance in Korea during 1985-1997 was calculated according to the surface balance method of the PARCOM guidelines and compared with Japanese N balance. The some differences were founded in the coefficients used on calculating N balance in two nations. Of the important parameters, which can make a big difference in balance, N input by organic fertilizers was not included in Korea different with Japanese, due to absence of reliable statistics and then made lower the input. Nitrogen destruction rate from livestock manure was adjusted differently with 15% in Korea but 28% in Japan. There was some difference in the conversion factors of livestock number into manure N quantity in two nations, but the gap was ignoble scale except beef cattle. Our manure N production rate of beef cattle might be evaluated to be so lower than Japanese. Biological N fixation by pulses was very higher in Korea than in Japan but scarcely affect the increase of total N input, due to small cultivation area. In contrast, N fixation rate by free-living organisms in Korean and Japanese wet paddies showed the big difference with 7.6 and $37.0kg\;ha^{-1}\;yr^{-1}$, respectively, and therefore $29.4kg\;ha^{-1}\;yr^{-1}$ of nitrogen was estimated to be more inputted in Japan. Although there are many points to be more specified and improved, still, Korean N balance was very high with $250-257kg\;ha^{-1}$ in the mid of 1990s, which was the second highest level in OECD countries and furthermore increased continuously during the investigation. In contrast in Japan, which has similar fanning system with Korea, N balance was lower with $130-158kg\;ha^{-1}$ and has decreased continuously since 1993. This high N balance was mainly due to a high usage of chemical fertilizers in our intensive fanning system and the fast increment of livestock feeding. Therefore, the more active action to decrease chemical fertilizer utilization and reduce livestock feeding density is required in the government and farmer sides.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.11a
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• pp.139-140
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• 2017

This technology employs a method of forming a single-ply PLUS waterproofing sheet layer comprised of applying a single-ply synthetic polymer layer on a vibrating structure (steel frame, RC) or an inclined surface by using a T joint lap-filling coil and an embedded metal coated sheet. The T - joint reinforcing lap-filling coil was used to block the ingress channel of the rainwater by applying the material in the vulnerable area where the three sides of the waterproof sheet overlapped. Conventional waterproofing techniques have a problem in that the waterproof sheet is pierced because the end portion of the waterproof sheet applied to the vertical portion is fixed by a nail, and the sealant applied to the end portion of the sheet cannot easily secure long-term waterproof durability due to the influence of the external environment. Therefore, the developed technology secured the waterproof durability against the vertical part by using the embedded metal sheet. In addition, automatic hot-air fusing is used to improve the quality of waterproof construction and point fixation method using fixed hardware. This is a technology that is not significantly restricted in the high degradation level regions of domestic waterproof construction environments in Korea such as low-temperature environment, wet floor.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.5
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• pp.342-351
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• 2014

The present paper investigates the performance of direct wet mineral carbonation technology to fix carbon dioxide ($CO_2$) from relatively high $CO_2$ concentration feeding gas using wollastonite ($CaSiO_3$)-water (and 0.46 M acetic acid) suspension solution. To minimize the energy consumed on the process, the carbonation in this work is carried out at atmospheric pressure and slightly higher room temperature. As a result, carbon fixation is confirmed on the surface of $CaSiO_3$ after carbonation with wollastonite-water suspension solution and its amount is increased according to the $CO_2$ composition in the feeding gas. The leaching and carbonation ratio of wollastonite-water suspension system obtained from the carbonation with 50% of $CO_2$ composition feeding gas is 13.2% and 10.4%, respectively. On the other hand, the performance of wollastonite-acetic acid in the same condition is 63% for leaching and 1.39% for carbonation.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.1
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• pp.97-105
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• 2008

Nitrogen budgets in Korea in 2005 were estimated using a mass balance approach. Major nitrogen fluxes were divided into three sections: cities, agricultural area, and forest. Nitrogen inputs were chemical and biological fixation, dry and wet deposition, imported food and feed, while crop uptake, volatilization, denitrification, leaching, runoff, and forest consumption were nitrogen outputs. Non-point source(NPS) pollution budgets were also estimated by mass balance approach. Annual total nitrogen inputs budgets were 1,442,254 ton$\cdot$yr$^{-1}$, and outputs were 814,415 ton$\cdot$yr$^{-1}$. Approximately 19.4% of nitrogen input leaked to river and seawater as NPS pollution. It contains nitrogen input 21 percent more than the previous research in 2002. Especially the change of government plans affect nitrogen budget. As a result, in the output field, the whole nitrogen amount due to landfill reduce from 20 percent to less than 1 percent.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.2
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• pp.103-112
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• 2011

The main objective of this research was to estimate Nitrogen budget of South Korea in 2008. Input-output budgets for nitrogen fluxes were categorized into three sections: cities, agricultural area, and forest. Chemical and biological fixation, dry and wet deposition, imported food and feed were used as the nitrogen input. Crop uptake, volatilization, denitrification, leaching, runoff, and forest consumption were used as the nitrogen outputs. Annual total nitrogen input was 1,294,155 ton/yr, and output was 632,228 ton/yr. Comparison with a previous research in 2005 indicates that nitrogen input was decreased by 1.9% due to the decrease in nitrogen fertilizer while nitrogen output was decreased by 6.3%. Non-point source (NPS) pollution was also estimated by mass balance approach, which increased by 22% than the previous research in 2005. The emission of nitrous oxide ($N_2O$) caused by denitrification was newly examined in this research. About 8,289 ton/yr of $N_2O$ was released from agriculture area and domestic wastewater treatment plant.