• 대한바이러스학회지
• /
• 제28권3호
• /
• pp.215-224
• /
• 1998

Cloning, sequencing and expressing in E. coli of the thymidine kinase (TK) gene of Herpes simplex virus type-1 (HSV-1) strain F was investigated. The TK gene, located in the BamHI 3.74 kb DNA fragment of the plasmid pHLA-12, was amplified by polymerase chain reaction (PCR). The 1,131 kb PCR product was cloned into the BamHI and EcoRI sites of pBacPAK9 plasmid and then named pBac-TK recombinant. The TK gene was subcloned into the BamHI and BglII sites of pQE-30, and named pQE-TK recombinant. The nucleotide sequence of the 1,131 kb TK gene was determined, and the GC content was 65.13%. There were deduced 367 amino acid residues with a total molecular weight of 43 kDa. The weight was confirmed by the protein produced by E. coli M15/pQE-TK on the SDS-PAGE and Western blot. The production of the TK protein in the IPTG induced cells was measured over 4 h. At the end of 1, 2 and 3 h the level increased by 146, 204 and 242%, respectively. The amount of the protein at the highest fraction purified with Ni-NTA resin chromatography was $0.68\;{\mu}g$ per ml. The soluble state TK protein was present in the cytoplasm. In these results the F strain was different in base sequence and amino acid sequence from that of the CL101 strain, which caused difference in their strains.

• 한국식품과학회지
• /
• 제30권1호
• /
• pp.218-223
• /
• 1998

우유 casein단백질을 alcalase로 가수분해시켰을 때, 생성되는 peptide 중 철분과 결합력이 있는 peptide인 IBP가 $immobilized\;Fe^{3+}\;affinity\;chromatography$에 의하여 용이하게 분리되었다. IBP의 분자량은 2,175 dalton이었으며 $pH\;6,\;37^{\circ}C$에서 1시간동안 일정량의 철분과 항온처리하였을 경우, 25, 50, 100 g/mL의 IBP는 각각 4.2, 5.7, 7.1 g의 철분을 가용화시키는 능력을 보였다. IBP는 proline (24.5 Mol%), lysine (15.7 Mol%), glutamic 또는 glutamine (14.9 Mol%) 등의 아미노산으로 구성되어 있었으며 이들의 N-terminal sequence는Met-Ala-Pro-Lys-His의 순으로 이루어져 있었다. 분자량, 아미산 조성, N-terminal sequence등의 결과를 종합해 보면 IBP는 casein 중 -casein의 아미노산 서열 $102{\sim}119$에 해당하는 18개의 아미노산으로 구성된 peptide임을 알 수 있었다.

• Animal cells and systems
• /
• 제5권4호
• /
• pp.313-317
• /
• 2001

The sequential assessment model describes a fight between two conspecific as an ongoing statistical sampling process, which makes it possible to predict fight length or repetition number of a behavioral element depending on relative RHP (resource holding potential: e.g. weight or fighting ability). We staged contests between males of common freshwater gobies to test some predictions of this model. Fights proceeded in a consistent sequence of phases. Most contests began with two contestants adopting lateral display, and then escalated to intense physical contacts. The length of contests was negatively correlated with weight difference between the contestants. The duration of complete phases was, however, independent of weight, and the prior information gained during complete phases did not appear to affect subsequent phases of the fight. Our results show that the contests of common freshwater gobies are well predicted by the sequential assessment model.

• 대한산업공학회지
• /
• 제12권2호
• /
• pp.1-11
• /
• 1986

This study is concerned on computerized analysis of COG, torques and EMG amplitudes in weight-lifting motion. The results show that; (1) torques on major joints show a rather consistent relationship with respect to the sequence of four distinct motions in weight-lifting, (2) analysis of EMG amplitudes is a sensitive measure of both athlete's skill and his potential capability, and (3) range of COG variations can be used as indicator of motion stability, existence of undesirable posture, and target muscle for intensive training. A computerized routine, which includes analyses on COG, EMG and torque, is a scientific tool for coaching athletes. In addition, an Expert System which includes CAD routine was developed in order to promote better understanding for athletes and coaches.

• ETRI Journal
• /
• 제26권5호
• /
• pp.413-422
• /
• 2004

This paper investigates the impacts of array weight errors (AWE) in an antenna array (AA) on a parallel interference cancellation (PIC) receiver in uplink synchronous and asynchronous direct sequence code division multiple access (DS-CDMA) systems. The performance degradation due to an AWE, which is approximated by a Gaussian distributed random variable, is estimated as a function of the variance of the AWE. Theoretical analysis, confirmed by simulation, demonstrates the tradeoffs encountered between system parameters such as the number of antennas and the variance of the AWE in terms of the achievable average bit error rate and the user capacity. Numerical results show that the performance of the PIC with the AA in the DS-CDMA uplink is sensitive to the AWE. However, either a larger number of antennas or uplink synchronous transmissions have the potential of reducing the overall sensitivity, and thus improving its performance.

• Journal of Information Processing Systems
• /
• 제6권1호
• /
• pp.79-90
• /
• 2010

A data stream is a massive unbounded sequence of data elements continuously generated at a rapid rate. The continuous characteristic of streaming data necessitates the use of algorithms that require only one scan over the stream for knowledge discovery. Data mining over data streams should support the flexible trade-off between processing time and mining accuracy. In many application areas, mining frequent itemsets has been suggested to find important frequent itemsets by considering the weight of itemsets. In this paper, we present an efficient algorithm WSFI (Weighted Support Frequent Itemsets)-Mine with normalized weight over data streams. Moreover, we propose a novel tree structure, called the Weighted Support FP-Tree (WSFP-Tree), that stores compressed crucial information about frequent itemsets. Empirical results show that our algorithm outperforms comparative algorithms under the windowed streaming model.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 1991년도 춘계학술발표대회 논문집
• /
• pp.225-236
• /
• 1991

The genes encoding the thermostable $\alpha$-amylase and maltogenic amylase from Bacillus lichenciformis were cloned and expressed in E. coli. The recombinant plasmid pTA322 was found to contain a 3.1kb EcoRI genomic DNA fragment of the thermostable $\alpha$-amylase. The cloned $\alpha$-amylase was compared with the B. licheniformis native $\alpha$-amylase. Both $\alpha$-amylase have the same optimal temperature of $70^{\circ}C$ and are stable in the pH range of 6 and 9. The complete nucleotide sequences of the thermostable $\alpha$-amylase gene were determined. It was composed of one open reading rame of 1,536 bp. Start and stop codons are ATG and TAG. From the amino acid sequence deduced from the nucleotide sequence, the cloned thermostable $\alpha$-amylase is composed of 483 amino acid residues and its molecular weight is 55,200 daltons. The content of guanine and cytosine is $47.46mol\%$ and that of third base codon was $53_41mol\%$. The recombinant plasmid, pIJ322 encoding the maltogenic amylase contains a 3.5kb EcoRI-BamHI genomic DNA fragment. The optimal reaction temperature and pH of the maltogenci amylase were $50^{\circ}C$ and 7, respectively. The maltogenic amylase was capable of hydrolysing pullulan, starch and cyclodextrin to produce maltose from starch and panose from pullulan. The maltogenic amylase also showed the transferring activity. The maltogenic amylase gene is composed of one open reading frame of 1,734bp. Start and stop codons are ATG and ATG. At 2bp upstream from start codon, the nucleotide sequence AAAGGGGGAA seems to be the ribosome-binding site(RBS, Shine-Dalgarno sequence). A putative promoter(-35 and-10 regions) was found to be GTTAACA and TGATAAT. From deduced amino acid sequence from the nucleotide srquence, this enzyme was comosed of 578 amino acid residues and its molecular weight was 77,233 daltons. The content of guanine and cytosine was $48.1mol\%$. The new recombinant plasmid, pTMA322 constructed by inserting the thermostable $\alpha$-amylase gene in the EcoRI site of pIJ322 to produce both the thermostable $\alpha$-amylase and the maltogenic amylase were expressed in the E. coli. The two enzymes expressed from E. coli containing pTMA322 was reacted with the $15\%$ starch slurry at $40^{\circ}C$ for 24hours. The distribution of the branched oligosaccharides produced by the single-step process was of the ratio 50 : 50 between small oligosaccharide up DP3 and large oligosaccharide above DP3.

• 한국정보과학회논문지:소프트웨어및응용
• /
• 제31권11호
• /
• pp.1474-1482
• /
• 2004

본 논문에서는 적응적 가중치 함수를 이용하여 블록 단위의 모션 벡터를 필터링하는 방법을 제안한다. 제안하는 방법에서는 먼저 인접한 영상을 받아 들여 가변적 크기의 블록 정합 방법을 이용하여 모션 벡터를 추출한다. 그리고 추출된 모션 벡터를 강건 예측에 적용하여 아웃라이어(outlier)를 제거함으로써 강건 예측에서 사용하는 동작 모델에 근접한 모션 벡터만을 추출한다. 제안된 적응적 강건 예측은 연속적인 시그모이드 가중치 함수를 사용하여 정상 자료와 아웃라이어의 소속 정도를 보다 효과적으로 표현한다. 또한, 최소화 기법의 반복 단계에서 잔여에러가 감소함에 따라 점진적으로 시그모이드 가중치 함수를 조율함으로써 정상 자료와 아웃라이어를 보다 유연하게 분리한다. 실험에서는 카메라의 동작이 포함된 비디오 데이타를 입력 받아 성능을 비교 분석함으로써 제안한 방법의 우수함을 보인다.

• 생명과학회지
• /
• 제15권5호
• /
• pp.734-738
• /
• 2005

Xylanase를 생산하는 알칼리 내성 Bacillus alcalophilus AX2000의 chromosomal DNA로부터 xylanase 유전자를 cloning하여 그 염기배열 순서를 결정한 다음 이로부터 유전자 발현에 관련된 구조를 분석하였다. Xylanase 유전자의 cloning을 위해 제한효소 PstI으로 절단한 B. alcalophilus AX2000의 chromosomal DNA와 pUC19을 ligation 시켜 E. coli $DH5\alpha$에 형질전환시킨 후 형질전환체 중에서 xylanase 활성을 나타내는 재조합 plasmid pXTY99를 분리하였다. 재조합 plasmid pXTY99은 pUC19의 PstI 부위 내에 7kb의 외래 DNA가 삽입 되 었다. Cloning된 xylanase 유전자(xynT)의 염기배열을 분석한 결과 유전자의 크기는 1,020 bp이었고 이는 340개의 아미노산으로 구성된 분자량 40 kDa의 poly-peptide를 coding하고 있었다. 이 염기배열은 AUG 개시 codon으로부터 각각 259와 282 base상류에 TACAAT의 -10 box와 GTTCACA인 -35 box로 추정되는 염기배열이 존재하였으며 ribosome 결합부위가 존재하였다. B. alcalophilus AX2000의 xylanase와 아미노산배열의 유사성이 가장 높은 xylanase는 Bacillus sp. N137과 B. stearothemophilus 21 유래의 xylanase로 각각 $61\%$와 $59\%$의 유사성을 나타내었다.

• 대한바이러스학회지
• /
• 제29권3호
• /
• pp.155-163
• /
• 1999

Hantaan virus (HTNV), the etiologic agent of hemorrhagic fever with renal syndrome (HFRS), belongs to the genus Hantavirus, and has three single negative stranded RNA genome segments. HTNV strain Howang isolated from the blood of severe case of Korean HFRS is more virulent than HTNV 76/118 and the M and S genome segments' nucleotide sequence of Howang strain showed 93.5% and 94% homology to each segment of HTNV 76/118. We have obtained 6533 nucleotides long sequence of the L genome segment of Howang strain using reverse transcriptase in conjunction with PCR amplification and compared to other hantaviruses. The messenger sense of the L segment contains one long single long open reading frame of 2151 amino acids, which encodes a deduced RNA dependent RNA polymerase of 246.4 kDa caculated molecular weight protein. The nucleotide sequence of the L segment of Howang strain shows 93%, 74%, 66%, 65% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively. The amino acid sequence of the L segment of Howang strain shows 99%, 85%, 68%, 68% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively.