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Antitumor Activity of CBT-AK5 Purified from Lactobacillus casei against Sarcoma-180 Infected ICR Mice (유산균의 배양물로부터 분리된 물질에 대한 암 유발 ICR Mice의 항암 효과)

  • Yeo, Moon-Hwan;Kim, Dong-Myung;Kim, Yong-Hwan;Kim, Jung-Hwa;Baek, Hong;Chung, Myung-June
    • Journal of Dairy Science and Biotechnology
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    • v.26 no.2
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    • pp.23-30
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    • 2008
  • This study implicated that the CBT-AK5 purified from Lactobacillus casei (LAFTI L26) which showed antitumor activity in ICR mice. Hence, ICR mice were inoculated intraperitoneally Sarcoma-180 as well as CBT-AK5. Then we observed the life span and tumor increment of those ICR mice. Here our studies showed effect on two different way of treatment as intraperitoneally and orally treated in Sarcoma-180 infected ICR mice. We found that intraperitoneally treatment of Sarcoma-180 and CBT-AK5 is more effective than orally fed. The life span of the ICR mice were highly reduced after the inoculation of Sarcoma-180. Those effects like increment of body weight, the growth of ascites and solid were inhibited significantly after the treatment of CBT-AK5 in Sarcoma-180 infected ICR mice. Finally these studies suggested that CBT-AK5 isolated form Lactobacillus casei showed excellent antitumor activity against Sarcoma-180 infected ICR mice.

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Effects of Conjugated Linoleic Acid (CLA) Precursor and Produce Supplementation on Egg Production, Egg Quality and Blood Immunological Parameters in Laying Hens (Conjugated Linoleic Acid (CLA) 전구체 및 정제 부산물의 첨가가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향)

  • Yoo, J.S.;Shin, S.O.;Cho, J.H.;Chen, Y.J.;Kim, H.J.;Huang, Y.;Kim, Y.J.;Hwang, K.Y.;Kim, J.W.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.34 no.3
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    • pp.173-180
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    • 2007
  • This study was conducted to evaluate conjugated linoleic acid (CLA) precursor and produce supplementation on egg production, egg quality and blood immunological parameters in laying hens. The total of 252 (32-wk) Hy-line brown commercial hens were used for 5 weeks. Dietary treatments included 1) CON (basal diet + oat 1% + soybean oil 1%), 2) WOS (basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS (basal diet+ whey 1% + soybean oil 1%), 4) WOCLA (basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA (basal diet + whey 1% + CLA produce 1%) and 6) FOCLA (basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%). For 4 weeks egg shell thickness was higher in WOS and FOCLA treatments than WOCLA and WCLA treatments (P<0.05). Egg weight was significantly increased (P<0.05) in WOS and FOCLA treatments compared to WS and WCLA treatments for 4 weeks. From 4 to 5 weeks yolk high, yolk color and haugh unit were significantly higher in FOCLA treatment than CON treatment (P<0.05). IgG concentration of blood was increased in WOS and FOCLA treatments compared to CON, WS and WOCLA treatments(P<0.05). In conclusion, fish oil, CLA produce and oat supplementation in laying hens diet improve egg shell thickness, yolk high, yolk color and haugh unit, also, CLA precursor supplementaion in laying hens diet improve IgG concentration of blood higher than CON treatment.

Diversification of Rice Quality for Processing. Physicochemical Characteristics and Inheritance of Floury Endosperm Mutants (특수 가공용 미질개발 : 분상질배유 돌연변이 계통의 이화학적특성과 유전)

  • Kim, Kwang-Ho;Koh, Hee-Jong;Lee, Jang-Hoon;Park, Sun-Zik;Heu, Mun-Hue
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.3
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    • pp.264-274
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    • 1993
  • This study was carried out to assess the agronomic characters and physicochemical properties of floury and chalky-endosperm mutant lines induced by chemical mutagen treatment to rice varieties, Hwacheongbyeo and IR24. Linkage analysis of a floury-endosperm gene was carried out using linkage testers. The grain size of brown rice of the mutants was smaller than that of the original varieties. The l, 000-grain and 1$\ell$ weight were lighter in the mutants compared with those in the original varieties. The compound starch granules in the endosperm cell of the mutants showed a loosely-packed crystalline structure. Amylose contents in mutants ranged from 16.9 to 28.5%. Crude protein contents of the mutants were not significantly different from the original rice variety, Hwacheongbyeo, but white core mutant(line 47106) derived from IR24 showed higher protein(l1.32%) compared with IR24(8.30%). The mutants showed slightly harder gel characteristics, and much lower viscosity in Amylograph than original varieties. Steamed rice-cakes from mutant lines showed greater volume than those from original varieties. During the process of alcohol fermentation, Brix in the mutants(especially floury mutants) decreased faster and the alcohol production after 10-day fermentation was much greater in the mutants than in the original varieties. Three different gene loci for floury endosperm characteristics were identified from the allelism test among mutant lines, and the genes were tentatively symbolized as flo-a, flo-b and flo-c, respectively. A floury gene, flo-a, was linked with lg(liguleless) gene in the linkage group N, with R.V. 5.76$\pm$1.72%.

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Determination of Freely Dissolved PAHs in Seawater around the Korean Peninsula Using High Speed Rotation-Type Passive Sampling Device (고속회전식 수동형 채집 장치를 이용한 한반도 주변해역에서의 자유용존상 PAHs 측정)

  • JANG, YU LEE;LEE, HYO JIN;JEONG, HAEJIN;JEONG, DA YEONG;KIM, NA YEONG;KIM, GI BEUM
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.26 no.1
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    • pp.37-48
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    • 2021
  • A new high speed rotation type-passive sampling device (HSR-PSD), which can rotate seawater at high speed and absorb easily and quickly the freely dissolved hydrophobic organic contaminants from seawater, was developed and then applied around the Korean Peninsula. Freely dissolved concentrations (Cfree) of polycyclic aromatic hydrocarbons (PAHs) were determined using the HSR-PSD with low density polyethylene (LDPE) sheets as a passive sampler. Furthermore, dissolved concentrations (Cdissolved) of PAHs in seawater were also obtained from high volume water sampling as a conventional method to account for actual bioavailability. When the LDPE sheets were rotated in the HSR-PSD at 900 rpm, PAHs with log KOW 3.4 ~ 5.2 were equilibrated between the LDPE and water in 5 hours. Although the high molecular weight PAHs with log KOW 5.6 ~ 6.8 was expected to be 2 to 30 days to reach the equilibrium, the Cfree of the PAHs at equilibrium could be corrected using performance reference compounds in 5 hours. Meanwhile, the total Cfree of PAHs were from 0.32 to 1.2 ng/L, which were higher than reported values in other oceans, but lower than in coastal water such as estuary, harbor, or shore. A bioavailability from the detected PAHs was highest at the sampling line near the dumping site of the Yellow Sea. Predicted residual concentrations in biota were relatively higher in offshore including the dumping site than in coastal regions.

The Effect of Natural Mineral Complex Supplementation on Production, Egg Quality and Blood Characteristic in Laying Hens (사료 내 천연 복합 미네랄 제제의 첨가가 산란계의 생산성, 계란 품질 및 혈액 성상에 미치는 영향)

  • Yoo, J.S.;Kim, J.D.;Cho, J.H.;Chen, Y.J.;Kim, H.J.;Kang, D.K.;Min, B.J.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.33 no.3
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    • pp.189-194
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    • 2006
  • This study was conducted to investigate the effects of natural mineral complex supplementation on egg production and characteristic in laying hens. A total of two hundred forty laying hens were randomly allocated into four treatments with ten replications for six weeks. Dietary treatments included 1) CON (control, basal diet) 2) M0.5 (basal diet + 3% chitosan + 0.5% natural mineral complex), 3) M1.0 (basal diet + 3% chitosan + 1.0% natural mineral complek) and 4) M1.5 (basal diet + 3% chitosan + 1.5% natural mineral complex). In the egg production, the M1.5 treatment was significantly higher than other treatments(P<0.05). However, egg weight was significantly higher in M0.5 treatment than CON and M1.0 treatments(P<0.05). Egg shell breaking strength was higher in M1.5 treatment than M0.5 treatment. Egg shell thickness was the highest in M1.5 treatment compared to other treatments(P<0.05). The hens 134 M0.5 diet were improved egg yolk color compared to those fed other diets(P<0.05). The Haugh unit, CON and M1.0 treatments showed significantly different results compared to those of M1.5 treatment(P<0.05). The M1.5 treatment had higher Ca and Fe concentration in blood and higher K concentration in yolk than CON(P<0.05). In conclusion, supplementation of natural mineral complex in laying hen diets influenced on egg production, egg shell qualify and mineral concentration in blood and yolk.

Breeding of Oriental Lily 'Pacific Wave' with Upward-facing and White Petals (상향 개화형 백색 오리엔탈나리 'Pacific Wave' 육성)

  • Rhee, Hye Kyung;Cho, Hae Ryong;Lim, Jin Hee;Kim, Mi Seon;Park, Sang Kun;Shin, Hak Ki;Joung, Hyang Young;Yae, Byeong Woo
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.299-303
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    • 2008
  • An Oriental lily cultivar 'Pacific Wave' was released in 2007 at National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Suwon, Korea. The crossing was made in 1999 between Oriental lily 'Simplon', an outward-facing and white colored cultivar, and 'Spinoza', pink colored cultivar. The first selection was done in 2003 with a line of 'O-03-16'. Multiplication and bulb growth, and performance test was conducted from 2004 to 2006. This selection was later on given as 'Pacific Wave' in 2007 at NHRI. Flowering time of 'Pacific Wave' in plastics house culture is mid June and grows average 115 cm. Flowers are upward-facing with 20.1 cm in diameter and white with yellow centered (RHS W155C + Y9A). Mean petal length and width is 12.2 cm and 4.2 cm, respectively. Leaves are 12.3 cm long, 2.9 cm wide. The throat color is green. It shows gray and purple stigma, and red brown pollen. The weight and size of bulb is 82.5 g and 19.6 cm, respectively. Year-round flowering can be by storing the bulb under -1 to $-2^{\circ}C$ conditions. It is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It is needed to control Botrytis disease in summer wet season.

Opportunity Tree Framework Design For Optimization of Software Development Project Performance (소프트웨어 개발 프로젝트 성능의 최적화를 위한 Opportunity Tree 모델 설계)

  • Song Ki-Won;Lee Kyung-Whan
    • The KIPS Transactions:PartD
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    • v.12D no.3 s.99
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    • pp.417-428
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    • 2005
  • Today, IT organizations perform projects with vision related to marketing and financial profit. The objective of realizing the vision is to improve the project performing ability in terms of QCD. Organizations have made a lot of efforts to achieve this objective through process improvement. Large companies such as IBM, Ford, and GE have made over $80\%$ of success through business process re-engineering using information technology instead of business improvement effect by computers. It is important to collect, analyze and manage the data on performed projects to achieve the objective, but quantitative measurement is difficult as software is invisible and the effect and efficiency caused by process change are not visibly identified. Therefore, it is not easy to extract the strategy of improvement. This paper measures and analyzes the project performance, focusing on organizations' external effectiveness and internal efficiency (Qualify, Delivery, Cycle time, and Waste). Based on the measured project performance scores, an OT (Opportunity Tree) model was designed for optimizing the project performance. The process of design is as follows. First, meta data are derived from projects and analyzed by quantitative GQM(Goal-Question-Metric) questionnaire. Then, the project performance model is designed with the data obtained from the quantitative GQM questionnaire and organization's performance score for each area is calculated. The value is revised by integrating the measured scores by area vision weights from all stakeholders (CEO, middle-class managers, developer, investor, and custom). Through this, routes for improvement are presented and an optimized improvement method is suggested. Existing methods to improve software process have been highly effective in division of processes' but somewhat unsatisfactory in structural function to develop and systemically manage strategies by applying the processes to Projects. The proposed OT model provides a solution to this problem. The OT model is useful to provide an optimal improvement method in line with organization's goals and can reduce risks which may occur in the course of improving process if it is applied with proposed methods. In addition, satisfaction about the improvement strategy can be improved by obtaining input about vision weight from all stakeholders through the qualitative questionnaire and by reflecting it to the calculation. The OT is also useful to optimize the expansion of market and financial performance by controlling the ability of Quality, Delivery, Cycle time, and Waste.

Agronomic Characteristics of A Mutant for Genic Male Sterility-Chalky Endosperm and Its Utilization on $F_1$ Hybrid Breeding System in Rice (벼 유전자적 웅성불임-심백 돌연변이체의 특성과 그를 이용한 일대잡종 육성체계)

  • ;Mun-Hue Heu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.684-696
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    • 1995
  • A mutant for chalky endosperm and genetic male sterility (GMS) was newly developed in rice. The two characters were found to be controlled by single recessive gene which has pleiotropic effect, indicating that chalky seeds should be GMS seeds in segregating populations. Chalky seeds showed the same shape and size as normal seeds. However, starch composition of central part of endosperm was looser and shape of starch granules was rounder compared with normal endosperm, resulting in significantly lower grain weight, absolute density and grain hardness in chalky grains than in normal ones. Amylose content and gel consistency of chalky grains were much lower and harder, respectively. Male sterile plants showed much shorter plant height, poorer panicle exsertion and lesser panicle number compared with normal plants. Microspore abortion stage in pollen developmental process was observed as before meiosis. Male sterility of the mutant was stable regadless of temperature and day length. A system breeding hybrid rice using this mutant was discussed, comp ring with other systems utilizing cytoplasmic-genic male sterility(CGMS) and environment sensitive GMS(EGMS). Separation of GMS seeds in mixed seed bulks by specific gravity (1.14∼1.16g / cm3) was successful about 85∼90%. But some mixed normal plants were seemed to be easily removed by the apparent difference in growth characters at seedling stage. The highest natural outcrossing rate of this GMS line was as 17.3 % in a plot treated with 2-row pollinator, I-row GMS, and GA3 + cutting of flag leaf + pollen-scattering by rope.

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The Varietal Comparison in Growth Characteristic and Yield of Vegetable Soybean (풋콩으로의 생육특성과 수량의 품종간 비교)

  • Lee, Seung-Su;Kim, Chang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.156-160
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    • 2008
  • This study was conducted to find on the screening of a optimal variety after compare with characteristics of a colored soybean recommended and a vegetable soybean by pure line isolation from the indigenous variety in Chungnam area. The budding period in results compared with a budding property of testing varieties between the recommended colored soybean 6 varieties and the indigenous 3 varieties, was showed that it was delayed by increasing of a grain weight but the budding rate was not significant. And the harvest period was the most early day at the black colored soybean plot at the 30th July and was the latest day at the native variety (Nonsan jaerae) at the 21 th October. The stem length was showed a wide range from 25 to 104 cm, and the Asan jaerae in this result was the highest on 104 cm, but the Geomjeongolkong was the lowest on 25 cm. The number of pod per plant was searched about 9 to 111 range, and the most numerous plot in this experimental varieties was the Ilpumgeomjeongkong as 111 number, on the other hand, the least variety was the Asan jaerae as 9 number. The rate of more than 2 seed per pod in the vegetable soybean property was the highest at the Geomjeongolkong about 82%. Additionally, the Hwangkuemkong and Geomjeongkong 1 was relatively the high levels as 78% and 73%, respectively. At the pod yield per $1000\;m^2$ (10a) was the most much as 1,567 kg at the Ilpumgeomjeongkong. Also, the Sunheukkong and the Galmikong was 1,468 kg, 1,397 kg, respectively. Therefore, the Ilpumgeomjeongkong, Sunheukkong, and Galmikong were proper varieties for the vegetable cultivation.

SSR Marker Related to Major Characteristics Affected Kernel Quality in Waxy Corn Inbred Lines (찰옥수수 자식계통의 주요 품질특성과 관련된 SSR마커)

  • Jung, Tae-Wook;Moon, Hyeon-Gui;Son, Beom-Young;Kim, Sun-Lim;Kim, Soon-Kwon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.185-192
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    • 2006
  • This experiment was conducted to assess genetic diversity of waxy corn inbred lines and to identify SSR markers related to major characteristics affected kernel quality for improving waxy corn $F_1$ hybrid with good quality. Diversity of 64 waxy com inbred lines was evaluated using 30 microsatellite markers. The 30 microsatellite markers representing 30 loci in the maize genome detected polymorphisms among the 64 inbred lines and revealed 225 alleles with a mean of 7.5 alleles per primer. The polymorphism Information content (PIC) value ranged from 0.14 to 0.87, with an average of 0.69. Based on Nei's genetic distances, the 64 inbred lines were classified into 9 groups by the cluster analysis. The group I included 26 inbred lines (41%), other groups included 3 to 9 inbred lines. One-way analysis of variance was conducted to identify significant relationship between individual markers and major characteristics that affect kernel quality. The analysis showed that umc1019 was related to amylopectin and crude protein content, me 1020 to amylopectin content and peak viscosity, and bnlg1537 to 100-kernel weight, kernel length, and kernel width.