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Effects of Conjugated Linoleic Acid (CLA) Precursor and Produce Supplementation on Egg Production, Egg Quality and Blood Immunological Parameters in Laying Hens

Conjugated Linoleic Acid (CLA) 전구체 및 정제 부산물의 첨가가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향

  • Yoo, J.S. (Department of Animal Resource & Science, Dankook University) ;
  • Shin, S.O. (Department of Animal Resource & Science, Dankook University) ;
  • Cho, J.H. (Department of Animal Resource & Science, Dankook University) ;
  • Chen, Y.J. (Department of Animal Resource & Science, Dankook University) ;
  • Kim, H.J. (Department of Animal Resource & Science, Dankook University) ;
  • Huang, Y. (Department of Animal Resource & Science, Dankook University) ;
  • Kim, Y.J. (Department of Food & Biotechnology, Korea University) ;
  • Hwang, K.Y. (Division of Biotechnology, Korea University) ;
  • Kim, J.W. (Department of Animal Resource & Science, Dankook University) ;
  • Kim, I.H. (Department of Animal Resource & Science, Dankook University)
  • 유종상 (단국대학교 동물자원과학과) ;
  • 신승오 (단국대학교 동물자원과학과) ;
  • 조진호 (단국대학교 동물자원과학과) ;
  • 진영걸 (단국대학교 동물자원과학과) ;
  • 김효진 (단국대학교 동물자원과학과) ;
  • 황염 (단국대학교 동물자원과학과) ;
  • 김영준 (고려대학교 식품생명공학과) ;
  • 황광연 (고려대학교 생명공학부) ;
  • 김정우 (단국대학교 동물자원과학과) ;
  • 김인호 (단국대학교 동물자원과학과)
  • Published : 2007.09.30

Abstract

This study was conducted to evaluate conjugated linoleic acid (CLA) precursor and produce supplementation on egg production, egg quality and blood immunological parameters in laying hens. The total of 252 (32-wk) Hy-line brown commercial hens were used for 5 weeks. Dietary treatments included 1) CON (basal diet + oat 1% + soybean oil 1%), 2) WOS (basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS (basal diet+ whey 1% + soybean oil 1%), 4) WOCLA (basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA (basal diet + whey 1% + CLA produce 1%) and 6) FOCLA (basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%). For 4 weeks egg shell thickness was higher in WOS and FOCLA treatments than WOCLA and WCLA treatments (P<0.05). Egg weight was significantly increased (P<0.05) in WOS and FOCLA treatments compared to WS and WCLA treatments for 4 weeks. From 4 to 5 weeks yolk high, yolk color and haugh unit were significantly higher in FOCLA treatment than CON treatment (P<0.05). IgG concentration of blood was increased in WOS and FOCLA treatments compared to CON, WS and WOCLA treatments(P<0.05). In conclusion, fish oil, CLA produce and oat supplementation in laying hens diet improve egg shell thickness, yolk high, yolk color and haugh unit, also, CLA precursor supplementaion in laying hens diet improve IgG concentration of blood higher than CON treatment.

본 연구는 CLA 전구체 및 정제 부산물의 급여가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향에 대하여 알아보기 위하여 시험을 실시하였다. 사양 시험은 32주령 ISA Brown 252수를 공시하였으며, 5주간 사양 시험을 실시하였다. 실험 설계는 1) CON(basal diet + oat 1% + soybean oil 1%), 2) WOS(basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS(basal diet+ whey 1% + soybean oil 1%), 4) WOCLA(basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA(basal diet + whey 1% + CLA produce 1%) 및 6) FOCLA(basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%)로 6개 처리를 하여 처리당 7반복, 반복당 6수씩 완전 임의 배치하였다. 난각 두께는 시험 4주에서 WOS 및 FOCLA 처리구가 WOCLA 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났으며(P<0.05), 난중은 시험 4주에서 WOS 및 FOCLA 처리구가 WS 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황고, 난황색 및 호우 유닛에 있어서 FOCLA 첨가구는 대조구와 비교하여 높게 나타났으며, 종료 시 IgG에 있어서 WOS 및 FOCLA 처리구가 대조구, WS 및 WOCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 결론적으로 어유, 정제 부산물 및 귀리의 첨가는 난각 두께, 난황고, 난황색 및 호우 유닛을 개선시키는 효과를 보여주었으며, CLA 전구체의 첨가 급여는 혈액 내 IgG의 함량을 대조구와 비교하여 증가시켰다.

Keywords

References

  1. Ahn DH, Sell JL, Jo C, Chamruspollert M, Jeffrey M 1999 Effect of dietary conjugated linoleic acid on the quality characteristics of chicken eggs during refrigerated storage. Poult Sci 78:922-928 https://doi.org/10.1093/ps/78.6.922
  2. Chamruspollert M, Sell JL 1999 Transfer on dietary conjugated linoleic acid to egg yolks of chickens. Poult Sci 78(8):1138- 1150 https://doi.org/10.1093/ps/78.8.1138
  3. Cook ME, Miller CC, Park Y, Pariza M 1993 Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poultry Sci 72:1301- 1305 https://doi.org/10.3382/ps.0721301
  4. Du M, Ahn DU, Nam KC, Sell JL 2000a Influence of dietary conjugated linoleic acid on volatile profiles, color and lipid oxidation of irradiated raw chicken meat. Meat Sci 56:387- 395 https://doi.org/10.1016/S0309-1740(00)00067-X
  5. Du M, Ahn DU, Sell JL 2000b Effect of dietary conjugated linoleic acid (CLA) and linoleic/linolenic acid ratio on polyunsaturated fatty acid status in laying hens. Poultry Sci 79:1749-1756 https://doi.org/10.1093/ps/79.12.1749
  6. Duncan DB 1955 Multiple range and multiple F tests. Biometrics 11:1-14 https://doi.org/10.2307/3001478
  7. Ha YL, Grimm NK, Pariza MW 1987 Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8:1881-1887 https://doi.org/10.1093/carcin/8.12.1881
  8. Haumann BF 1996 Conjugated linoleic acid offers research promise. INFORM 7:152-159
  9. Jones S, Ma DWL, Robinson FE, Field CJ, Clandinin MT 2000 Isomers of conjugated linoleic acid (CLA) are incorporated into egg yolk lipids by CLA-fed laying hes. J Nutr 130: 202-205
  10. Joo ST, Lee JI, Ha YL, Park GB 2002 Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation color and water-holding capacity of pork loin. J Ani Sci 80:108-112 https://doi.org/10.2527/2002.801108x
  11. Kepler CR, Hirons KP, Mcneil JJ and Tove SB 1966 Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. J Biological Chemistry 241: 1350-1354
  12. Lee KN, Kritchevsky D, Pariza MW 1994 Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19- 25 https://doi.org/10.1016/0021-9150(94)90034-5
  13. Lee KN, Storkson JM, Pariza MW 1995 Dietary conjugated linoleic acid changes fatty acid composition in different tissues by decreasing monounsaturated fatty acids. p.183 in: IFT Annual Meeting Book of Abstracts, Anaheim, CA
  14. Miller CC, Park Y, Pariza MW, Cook ME 1994 Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biophy Res Commu 198:1107-1112 https://doi.org/10.1006/bbrc.1994.1157
  15. Miner JL, Cederberg CA, Nielsen MK, Chen X, Baile CA 2001 Conjugated linoleic acid (CLA), body fat, and apoptosis. Obe Res 9:129-134 https://doi.org/10.1038/oby.2001.16
  16. Nicolosi RJ, Courtemanche KV, Laitinen L, Scimeca JA, Huth PJ 1993 Effect of feeding diets enriched in conjugated linoleic acid on lipoproteins and aortic atherogenesis in hamsters. Circulation 88 suppl:2458 https://doi.org/10.1161/01.CIR.88.5.2458
  17. O'-Quinnn PR, Nelssen JL, Goodband RD, Unruh JA, Woodworth JC, Smith JS, Tokach MD 2000 Effects of modified tall oil versus a commercial source of conjugated linoleic acid and increasing levels of modified tall oil on growth performance and carcass characteristics of growing-finishng pigs. J Ani Sci 78:2359-2368 https://doi.org/10.2527/2000.7892359x
  18. Park JK, Kim JY, Lee BH, Lim JJ, Chung BH 2003 CLA treatment effect on immune suppressive effect of corticosteroid in mouse. J Vet Clin 20(1):79-85
  19. Park YH, Alright KJ, Liu W, Storkson JM, Cook ME, Pariza MW 1997 Effect of conjugated linoleic acid on body composition in mice. Lipids 32(8):853-858 https://doi.org/10.1007/s11745-997-0109-x
  20. SAS 1996 SAS user's guide: Statisics. SAS Inst, Inc., Cary, NC
  21. Sugano M, Tsujita A, Yamasaki M, Noguchi M, Yamada K 1998 Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats. Lipids 33: 521-7 https://doi.org/10.1007/s11745-998-0236-4
  22. Thiel RL, Sparks JC, Wiegand BR, Parrish FC, Ewan RC 1998 Conjugated linoleic acid improves performance and body composition in swine. p.127 in: Midwestern Section ASAS and Midwest Branch ADSA 1998 Meeting, Des Moinse, IA
  23. West DR, DElany JP, Camet PM, Blohm F, Truett AA, Scimeca J 1998 Effect of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol R667- 672
  24. 권순관 2002 계란 난황 내 Arachidonic acid 및 conjugated linoleic acid 이행에 관한 연구. 건국대학교 대학원 박사학위논문
  25. 신경훈 2005 Conjugated linoleic acid와 ${\omega}$-3계열 지방산의 혼합 첨가 급여가 양계 산물로의 이행 및 지질 대사에 미치는 영향. 건국대학교 대학원 석사학위논문
  26. 이희애 1997 오메가 3계 지방산 강화 식품류의 연구개발 동향. 한국식품영양과학회지 26(1):161-174
  27. 주선태 이상조 허선진 하정기 하영래 박구부 2002 Conjugated linoleic acid(CLA)의 급여가 계란의 품질에 미치는 영향. 한국가금학회지 22(3):252-258
  28. 황보종 장종수 정일병 이병석 김동운 조성백 김희도 배해득 손진혁 홍의철 최낙진 2005 산란계 사료내 CLA 함유 Oil (CLAzen$80^{\circledR}$) 첨가가 난황내 지방산 조성에 미치는 영향. 한국가금학회지 32(1):35-41