• KSBB Journal
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• v.32 no.1
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• pp.54-62
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• 2017

This study aimed to estimate the measurement uncertainty related to determination of hyaluronic acid used as a dietary supplement by high-performance liquid chromatography. According to the guidelines of the Association of Official Analytical Chemists, the analytical processes for determination of hyaluronic acid were performed. And the measurement uncertainty obtained during the analytical processes were expressed in accordance with mathematical/ statistical guidances of GUM (Guide to the Expression of Uncertainty in Measurement) & EURACHEM (Focus for Analytical Chemistry in Europe) for the analytical operations. For the uncertainty in measurement produced based on this analytical method, the expanded uncertainty was calculated by using the relative standard uncertainty between analytical results and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc). In the results of 95% confidence interval, it was calculated that the uncertainty in measurement was $57.75{\pm}8.76{\mu}g/kg$ (k=2.0). Therefore, it showed that the measurement uncertainty obtained by this analytical method influences on 15.2% of the contents of hyaluronic acid as the analytical results.

• Journal of Life Science
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• v.21 no.4
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• pp.610-612
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• 2011

We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at $50^{\circ}C$ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-$300\;{\mu}M$). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.

• Journal of the Korean Chemical Society
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• v.21 no.1
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• pp.44-52
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• 1977

The effects of specific surface area and pore size of dialysis membrane on the dialysis were studied. It was confirmed that the intermittent sampling method(in which any volume of dialyzing solution is sampled intermittently at every moment after any duration of the dialysis experiment) is suitable for the determination of a dialysis coefficient. It was also confirmed that the diffusion coefficient of a electrolyte through membrane is smaller than the free diffusion coefficient though the pore size of dialysis membrane is extremely large.

• Geomechanics and Engineering
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• v.30 no.4
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• pp.393-399
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• 2022

The Segregation Potential (SP) is one of the most widely used predictors of frost heave in cold regions. Laboratory step-freezing tests determining a representative SP at the onset of the formation of the last ice lens (near the thermal steady state condition) can predict susceptibility to frost heave. Previous work has proposed empirical semi-log fitting for determination of the representative SP and applied it to several fine-grained soils, but considering only frost-susceptible soils. The presence of fines in coarse-grained soil affects frost susceptibility. Therefore, it is required to evaluate the applicability of the empirical semi-log fitting for both frost-susceptible and non-frost-susceptible soils with fines content. This paper reports laboratory frost heave tests for fines contents of 5%-70%. The frost susceptibility of soil mixtures composed of sand and silt was classified by the representative SP, and the suitability of the empirical semi-log fitting method was assessed. Combining semi-log fitting with simple laboratory frost heave testing using a temperature-controllable cell is shown to be suitable for both frost-susceptible and non-frost-susceptible soils. In addition, initially non-frost-susceptible soil became frost susceptible at a 10%-20% weight fraction of fines. This threshold fines content matched well with transitions in the engineering characteristics of both the unfrozen and frozen soil mixtures.

• Geomechanics and Engineering
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• v.29 no.5
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• pp.549-565
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• 2022

Severe acid rains can be a major source for geotechnical and environmental problems in any soil depending on the acid type and concentration. Hence, this study investigates the individual severe effects of sulfuric, hydrochloric and nitric acids on the geotechnical properties of real field soil through a series of experimental laboratory tests. The laboratory program consists of experimental tests such as consistency, compaction, unconfined compression, pH determination, electrical conductivity, total dissolved salts, total suspended solids, gypsum and carbonates contents. The experimental tests have been performed on the untreated soil and individual acid treated soil for acid concentrations range of 0% to 20% by weight. In addition, a unique hyperbolic mathematical model has been used to predict significant geotechnical characteristics for acid treated soil. The plastic and liquid limits and optimum moisture content have been increased under the effect of all the used acids whereas the maximum dry density and unconfined stress-strain behavior have been decreased with increasing the acid concentrations. Moreover, the used hyperbolic mathematical model has predicted all the geotechnical characteristics very well with a very high coefficient of determination (R²) value and lowest root mean square error (RMSE) estimate.

• Korean Journal of Construction Engineering and Management
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• v.4 no.4 s.16
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• pp.212-219
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• 2003

In construction project planning and control, a cost model performs a critical role such as cost determination on a contract stage and cost tracing. The model can maximize owner's profit and value within the project budget and optimize cost management works on overall construction implementation stages. A BoQ(Bill of Quantities) generally adopted in a unit price contract has been applied as an important tool for cost control and forecast. However a previous cost model based on the BoQ has shown limitations in that it requires too detailed information and heavy manpower on cost management and difficulty in keeping relationship with construction planning, scheduling and progress management. The each cost items and unit prices which constitute of construction works are individually very important management factors but the relative weight for each items and prices have a difference on the contents and conditions of each conditions of each construction works. In consideration of this structural mechanism of cost determination, this research is aimed at examining the critical factors affecting the construction cost determination and propose and verify a new cost forecasting model which is more simple and efficient and also keeps the accuracy of cost management.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.572-579
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• 2017

Determination of the wood content in wood plastic composite (WPC) is crucial to form reliable WPC market. WPC with simple formulation consisting of only two components (wood flour and polypropylene) was examined using thermogravimetric analysis (TGA) and accelerator mass spectrometry (AMS) for determining wood content in the WPC. TGA method using derivative peak temperature (DTp) of polypropylene under low heating rate ($5^{\circ}C/min$) showed more reliable calibration curve and lower error factor compared to method of using the percentage of weight loss of wood flour. In addition, AMS using bio-based carbon content showed greater reliability for the determination of wood content in the WPC in comparison with the TGA method.

• Microbiology and Biotechnology Letters
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• v.19 no.6
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• pp.598-603
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• 1991

An antifungal mixture of six members (component A to F), KRF-001 produced by Bacillzts subtilis subsp. krictiensis was isolated from the fermentation broth. Molecular weight of component A to F was determined by FAB-MS to be 1042, 1056, 1056, 1070, 1070 and 1084 respectively. Various instrumental analyses (amino acid analysis, GC-MS, $^1H-NMR, ^1HH$ COSY NMR) revealed that the mixture was a homologous cyclic peptide composed of each one mole of glutamine, proline, tyrosine, serine, unusual $\beta$-amino acid and three moles of asparagine. The structural differences of component A to F were found in carbon number and terminal structure of the unusual $\beta$-amino acid. After determination of the sequence and stereochemistry of those amino acids, the tentative structure of KRF-001 was determined.

• Korean Journal of Veterinary Research
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• v.47 no.2
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• pp.139-145
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• 2007

Measurement uncertainty could play an important role in the assessment of test results in laboratories and industries. We investigated measurement uncertainties possibly included in determination of flubendazole, a benzimidazole anthelmintic, in pork by HPLC. The concentration of flubendazole was 62.69 ng/g in a sample of pork. Uncertainty was estimated in the analytical procedure of flubendazole. A model equation was made for determination of flubendazole in pork. The four uncertainty components such as weight of sample, volume of sample, calibration curve, and recovery were selected to estimate measurement uncertainties. Standard uncertainty was calculated for each component and all the standard uncertainties were combined. The combined standard uncertainty was expanded to a sample population as an expanded uncertainty. The expanded uncertainty was calculated using k value on Student's t-table and effective degrees of freedom from Welch-Satterthwaite formula. The expanded uncertainty was calculated as 3.45 with the combined standard uncertainty, 1.584 6 and the k value, 2.18. The final expression can be ($62.69{\pm}3.45$) ng/g (confidence level 95%, k = 2.18). The uncertainty value might be estimated differently depending on the selection of the uncertainty components. It is difficult to estimate all the uncertainty factors. Therefore, it is better to take several big effecting components instead of many small effecting components.

• Korean Journal of Applied Biomechanics
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• v.24 no.4
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• pp.417-423
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• 2014

The purpose of this study was to compare two saddle height determination methods by the effectiveness of pedal reaction force. Ten male subjects (age: $24.0{\pm}2.4years$, height: $175.1{\pm}5.4cm$, weight: $69.3{\pm}11.1kg$, inseam: $77.8{\pm}4.5cm$) participated in three minutes, 60 rpm cycle pedaling tests with the same load and cadence. Subject's saddle height was determined by $25^{\circ}$ knee flexion angle (K25) when the pedal crank was at the 6 o'clock position (knee angle method) and 97% (T97), 100% (T100), 103% (T103) of trochanter height (trochanteric method). The RF (resultant force), EF (effective force), and IE (index of effectiveness) were compared by measuring 3D motion and 3-axis pedal reaction force data during 4 pedaling phases (phase1: $330^{\circ}-30^{\circ}$, phase2: $30^{\circ}-150^{\circ}$, phase3: $150^{\circ}-210$, phase4: $210^{\circ}-330^{\circ}$). Results showed that there were significant differences in EF at phase1 between T97 and K25, in EF at phase4 between T100 and T103, in IE at total phase between T97 and K25, between T100 and T103, in IE at phase1 & phase2 between T97 and K25. There was higher IE in the K25 than any other saddle heights, which means that K25 was better pedaling effectiveness than the trochanteric method. Therefore it was suggested the saddle height as 103.7% of trochanter height that converted from K25.