[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5352/JLS.2011.21.4.610

Spectrophotometric Assay for Determination of Chlorogenic Acid Using Green Pigment Formation and Quantitative Analysis of Chlorogenic Acid in Blueberry Leaf

Chung, Dong-Min (Bioindustrial Process Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology)
Chung, Young-Chul (Department of Food Nutrition, International University of Korea)
Chun, Hyo-Kon (Bioindustrial Process Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology)

Publication Information

Journal of Life Science / v.21, no.4, 2011 , pp. 610-612 More about this Journal

Abstract

We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at $50^{\circ}C$ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0- $300\;{\mu}M$ ). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.

Keywords

Blueberry leaf; chlorogenic acid; green pigment; amino acid; 96 well plate;

Citations & Related Records

Reference

1	Clifford, M. N. 2000. Chlorogenic acid and other cinnamates-nature, occurrence, dietary burden, absorption and metabolism. J. Sci. Food Agric. 80, 1033-1043. DOI
2	Mateos, R., L. Goya, and L. Bravo. 2006. Uptake and metabolism of hydroxycinnamic acids (chlorogenic, caffeic, and ferulic acids) by HepG2 cells as a model of the Human Liver. J. Agric. Food Chem. 54, 8724-8732. DOI
3	Politir, J. 1947. Sur une nouvelle methode concernant la localization dans les plantes. Compt. Rend. 225, 954-956.
4	Rice-Evans, C. A., N. J. Miller, and G. Paganga. 1996. Structure antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Biol. Med. 20, 933-956. DOI
5	Uritani, I. and K. Muramatsu. Studies on the phytopathological chemistry of black-rottin potato. Part 2. Some factors concerning the color changes of the injured part. Nippon Nogei Kagaku Kaish 27, 781-789, 1953. DOI
6	Yabut, G., Y. Koizumi, K. Namiki, M. Hida, and M. Mamiki. 2001. Structure of green pigment formed by the reaction of caffeic acid esters (or chlorogenic acid) with a primary amino compound. Biosci. Biotechnol. Biochem. 65, 2121-2130. DOI
7	Chen, J. H. and C. T. Ho. 1997. Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. J. Agric. Food Chem. 45, 2374-2378. DOI