• Title/Summary/Keyword: water soluble extract

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Fructans from Renga Renga Lily (Arthropodium cirratum) Extract and Frutafit as Prebiotics for Broilers: Their Effects on Growth Performance and Nutrient Digestibility

  • Vidanarachchi, J.K.;Iji, P.A.;Mikkelsen, L.L.;Choct, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.5
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    • pp.580-587
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    • 2010
  • An experiment was conducted to evaluate the effect of dietary water-soluble carbohydrate extract from Renga renga lily (Arthropodium cirratum) and a commercial product, $Frutafit^{{\circledR}}$ (both fructans) on the performance, organ weights, ileal digestibility and gut morphology of male Cobb broiler chickens. There were six treatment groups: a negative control with no supplements, a positive control supplemented with 45 ppm Zn-bacitracin, and four test diets each supplemented with Renga renga lily extract or Frutafit at 5 or 10 g/kg diet. Supplementation with low levels of Renga renga lily extract and Frutafit in the diet did not affect productive parameters, whereas the inclusion of a high level of Frutafit had a negative effect on BWG and FI compared with birds fed the negative control diet. The addition of an antibiotic to the diet significantly improved (p<0.05) the BWG and FCR of broilers. Apparent ileal digestibility of dry matter, starch, protein and fat was not affected (p>0.05) by supplementation with both levels of lily extract and the low level of Frutafit. The apparent ileal digestibility of dry matter, protein and fat was decreased (p<0.05) by the high level of Frutafit. The apparent metabolisable energy (AME) of the diets fed the high level of Frutafit was approximately 0.2 MJ/kg DM lower than that of the negative control group. The addition of Zn-bacitracin increased (p<0.05) the apparent ileal digestibility of fat. The relative weight of the liver was higher (p<0.05) in broilers supplemented with the high level of Frutafit than for negative control birds at 14 and 35 d of age. Feeding Renga renga lily extract or Frutafit had no effect on the gut morphology of birds on d 14 and 35. It can be concluded that dietary inclusion of fructans from the two sources used in this study affected broiler performance differently and in a dose-dependent manner.

Anti-Inflammatory Effect of Germinated Mung Bean and Hairdye Applications (발아 녹두 추출물의 항염증 효능 및 염모제 응용)

  • Kim, Eun-Seok;Jeong, Noh-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.1
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    • pp.23-30
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    • 2014
  • In this study, a research of anti-inflammatory effect and application for cosmetics after extracting of germinated mung bean. Germinated mung bean extract was showed high NO constraining effect compared with $250{\mu}g/m{\ell}$ concentrated mung bean extract and germinated mung bean extract. Also shown is the highest nitrogen oxide inhibiting effect that using 1,3-butylene glycol when comparing the results of extraction conditions of a water-soluble, and extracted mung bean germinated for 5 days. The result of applying the germinated mung bean extract hairdye inflammatory reaction happens many showed nitrogen oxides higher inhibitory effect than that hair dye containing germinated mung bean extract is not included. Therefore, germinated mung bean extract is expected to the abirritant and anti-inflammatory agent as the material for cosmetic.

Effect of Chicory Extract on Triglyceride Metabolism in Rats (흰쥐의 중성지질 대사에 미치는 치커리 추출물의 영향)

  • Cha Jae-Young;Park Chae-Kyu;Kang Ho Young;Cho Young-Su
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.613-618
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    • 2005
  • We investigated the effect of chicory (Chicorium intybus) extract on triglyceride concentration and microsomal triglyceride transfer protein (MTP) activity in rats. The effect of water-soluble extract of chicory fed at the $2.0\%\;and\;4.0\%(w/w)$ levels for 2 weeks on the concentration of serum triglyceride and the activity of hepatic microsomal riglyceride transfer protein (MTP) was investigated in male Sprague-Dawley rats. The triglyceride concentrations in serum of the chicory extract fed groups were significantly lower than in the control group. MTP activity, known to be essential for the assembly/secretion of apolipoprotein B-containing lipoproteins, was also significantly lower in the chicory extract groups than in the control group. The concentrations of other lipids in serum and liver and the activity of phosphatidate phosphohydrolase, the rate-limiting enzyme in triglyceride synthesis, showed no significant differences among in the chicory fed groups. These results indicate that dietary chicory extract decrease hepatic MTP activity and serum triglyceride concentration, and therefore reduces hepatic lipoprotein assembly and secretion.

An Antitumor Constituent of the Cultured Mycelia of Hydnum repandum (턱수염 버섯 배양(培養) 균사(菌絲)의 항종양(抗腫瘍) 성분(成分))

  • Chung, Kyeong-Soo;Choi, Eung-Chil;Kim, Byong-Kak;Kim, Yang-Sup
    • The Korean Journal of Mycology
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    • v.11 no.2
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    • pp.91-95
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    • 1983
  • To investigate antitumor constituents of Hydnum repandum, which belongs to edible basidiomycetes, the mycelia separated from the carpophore were shake-cultured and subjected to hot water extraction. The extract was concentrated under vacuum and mixed with a three-fold volume of 95% ethanol to yield precipitates. The water soluble fraction of the precipitates was dialyzed and then lyophilized to yield a water soluble protein-polysaccharide fraction (= WPPF). This exerted antitumor activity against sarcoma 180 implanted in ICR mice. When administered i.p. at the dose level of 20mg/kg once daily for ten consecutive days, it showed an inhibition ratio of 54.3%. WPPF was found to be composed of a polysaccharide moiety (42% of WPPF) and a protein moiety (28% of WPPF) when determined by colorimetric method using anthrone reagent and Folin's phenol reagent. The polysaccharide moiety of WPPF was found to contain glucose (57.4%), mannose(19.3%), galactose (10.8%), xylose (6.8%), and fucose (5.7%), when the methanolysate of WPPF was analysed by GLC method.

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Safety Evaluation on Mutagenicity of White Layer Cake Containing Gamma-Irradiated Egg White (감마선 조사된 난백 함유 White Layer Cake의 돌연변이원성 평가)

  • 김미정;이주운;서지현;송현파;육홍선;최정미;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.7
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    • pp.1172-1175
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    • 2003
  • Mutagenicity of white layer cake including 20 kGy-gamma irradiated egg white manufactured as a research on the practical approaches of gamma irradiation for the reduction of egg allergy was evaluated by Salmonella typhimurium reversion assay (Ames test). The water-soluble and organic solvents mixture of methanol: chloroform (2 : 1)-soluble fractions of the white layer cake including 20 kGy-gamma irradiated egg white were examined in S. typhimurium TA98 and TA100. Both with and without metabolic activation, the number of revertant colonies were not increased in each extract compared with negative controls. No significant difference in the formation of the colonies was observed at the non-irradiated and 20 kGy-irradiated samples. The results indicate that there is no evidence of mutagenicity in white layer cake including 20 kGy-gamma irradiated egg white.

In vitro Screening of Dietary Factors on Buckwheat(Fagopyrum esculentum Moench) Influencing the Regulation of Blood Pressure, Glucose and Cholesterol Level (혈압, 혈당 및 콜레스테롤농도 조절에 영향을 주는 식이 메밀인자의 in vitro 검정)

  • 최용순;김복란;김려화;이병훈;심태흠;이상영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.2
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    • pp.280-287
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    • 2000
  • Generally, buckwheat has been regarded as a crop of secondary importance in many countries. In vitro functionalities of buckwheats as a food were evaluated in this study. Five of buckwheat cultivars were extracted with methanol, and the extractant were dried and lyophilized, separately. Or water soluble buckwheat components were digested with the commercial enzymes and the obtained protein hydrolysate was again fractionated by acid precipitation. The antioxidant capacity of the methanol extracts determined using Fe2+-ascorbic acid system was dependent ont the cultivars: The extract of Suwon 4 showed 3.3 times stronger activity than ascorbic acid in terms of IC50. Also, the extracts of buckwheats inhibited efficiently the activities of $\alpha$-amylase and lens aldose reductase. Buckwheat soluble protein or rutin suppressed the in vitro activities of angiotensin-converting enzyme, and the inhibitory degree depended largely on the cultivars. Buckwheat proteins exerted higher hydrophobicity being related to the sterol binding capacity than casein. The results suggested that buckwheat seeds may be desirable and functional food resources in human living in current society.

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Development of New Products and High Value Added Biopolymer from Softwoods by Chemical Modification - Quantitative Variation of Water-soluble Extracts from Coniferous Barks for Tannin-based Adhesives - (화학가공에 의한 침엽수재의 품질귀화 및 고부가 Biopolymer 개발 (I) - 타닌접착제를 위한 침엽수 수피의 수용성 추출물의 양적 변이 -)

  • Cho, Nam-Seok;Han, Gyu-Seong
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.4
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    • pp.1-6
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    • 1996
  • Extracts from bark have been studied with a view to producing water-proof wood adhesives. Lately many softwoods, such as radiata pine from New Zealand and larch from Siberia, Russia, have been imported and utilized, and their residual barks would be expecting as potential raw materials for something useful chemicals. The great effort toward utilization of bark extractives as a chemical feedstock has been made on a worldwide level. However few report has been done for the utilization of tree bark extractives in Korea. Hot-water extracts were prepared from barks of Japanese larch(Larix leptolepis). Siberian larch(Larix gmelinii) and Radiata pine(Pinus radiata). The effect of various factors, such as particle size, liquor ratio, extraction temperature, and reaction time, on the extractive yields was discussed. Particle sizes affected the hot-water extractives: the finer the particle size, the higher extractives and extract efficiency. Higher temperature and higher liquor ratio were more effective. Extractives from Japanese larch were relatively less than those from Siberian larch and Radiata pine barks. Formaldehyde precipitates was the highest in extractives of Radiata pine barks. It could be concluded that Siberian larch bark was the best raw material for tannin adhesives, because its extractive yield was higher than those of the other barks.

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Dyeing of Silk Fabric with Aqueous Extract of Cassia tora L. Seed - focusing on the mordanting and dyeing mechanisms - (결명자 색소 추출액에 의한 견직물 염색 -매염 및 염착 mechanism을 중심으로-)

  • Dho Seong Kook;Kang In A
    • Textile Coloration and Finishing
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    • v.17 no.2 s.81
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    • pp.10-18
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    • 2005
  • Silk fabrics mordanted with $Fe^{2+},\;Ni^{2+},\;and\;Cu^{2+}$ were dyed with the aqueous extract of Cassia tora L. seed which was known to include water soluble colorant kaempferol, one of flavonol compounds. Kaempferol can react with free radicals and chelate transition metal ions, which is thought to catalyze processes leading to the appearance of free radicals and have antioxidant activity. In relation to the coordinating and chelating mechanism of the ions with the silk protein and kaempferol, reasonable conclusions should be made on the colorant uptake and the water fastness of the fabric. The amount of the colorant on the fabric was in the order of $Fe^{2+}>Ni^{2+}>Cu^{2+}$. In case of dyeing through coordinaiton bonds between transition metal ions and silk protein and colorants, it was thought that the ions with the smaller secondary hydration shell, the higher preference to the atoms of the ligand coordinated, and the suitable bonding stability for the substitution of primarily hydrated water molecules for colorants led to the higher colorant uptake. The water fastnsess of the fabric was in the order of $Fe^{2+}>Cu^{2+}>Ni^{2+}$. It should be reasonable to choose transition metal ions with weak and strong tendency to the ionic and the coordination bond, respectively, to the carboxylate anion of the silk protein. Although further research needs to be done, the conclusions above may be generally applied to the natural dyeing through the coordination bond mechanism between transition metal ions and colorants and substrates.

Production of Water-Solubled Pigment from Mycelial Culture of Cordyceps scarabaeicola KEFC-C252 and Its Antimutagenic Effect (Cordyceps scarabaeicola KEFC-C252의 균사체 배양에 의한 수용성 색소의 생산과 색소의 항돌연변이 효과)

  • 이현우;손준형;최종환;예병일;신운섭;김중배;김현원
    • Microbiology and Biotechnology Letters
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    • v.28 no.2
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    • pp.111-116
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    • 2000
  • Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at $26^{\circ}C$ for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TAlOO.

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Antimicrobial, Anti-inflammatory, and Anti-oxidative Effects of Water- and Ethanol-extracted Brazilian Propolis

  • Kim, Kee-Tae;Yeo, Eun-Ju;Han, Ye-Sun;Nah, Seung-Yeol;Paik, Hyun-Dong
    • Food Science and Biotechnology
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    • v.14 no.4
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    • pp.474-478
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    • 2005
  • Because it possesses anti-inflammatory, antifungal, antiviral, and tissue regenerative properties, propolis has been used for thousands of years in folk medicine for multiple purposes. Although the antimicrobial activity of propolis has already been demonstrated, very few studies have been conducted on bacteria of clinical relevance in dentistry. The aim of this study is to evaluate the antimicrobial, anti-inflammatory, and anti-oxidative activities of 0.1% and 1.0% propolis, both of water-extracted (proAQ) and ethanol-extracted (proAL) propolis, for industrial applications. In studies of antimicrobial activity, the growth of Staphylococcus aureus ATCC 35556, Salmonella enteritidis ATCC 12021, Escherichia coli O157:H7, and Candida parapsilosis KCCM 35428, all general food or clinical pathogens, were tested. The culture medium used was trypticase soy broth including 0.6% yeast extract; after 6 hr of incubation, the turbidities were measured at 620 nm with a spectrophotometer. The results indicate that the antimicrobial effects of both 1.0% proAQ and 1.0% proAL were greater against the growth of S. aureus ATCC 35556 and C. parapsilosis KCCM 35428 rather than those of S. enteritidis ATCC 12021 and E. coli O157:H7. Additionally, it appears that the anti-inflammatory effects of proAL are greater than those of proAQ. The anti-inflammatory effects were evaluated by measurement of the inhibition of hyaluronidase activity in vitro. At a 1% concentration, the anti-inflammatory effects of proAL were greater than those of proAQ. Finally, the anti-oxidative effects of 1% and 10% solutions of each extract sample were measured according to the TBA method at $40^{\circ}C$ for 1, 2, 3, and 5 days and were compared with 1.0% BHT. The results indicate that the anti-oxidative effects at 0.1% for both proAQ and proAL were not significantly different than the anti-oxidative effects at 1.0% BHT (p<0.05). Thus, it appeared that the alcohol-extracted propolis had greater antimicrobial, anti-inflammatory, and anti-oxidative effects than the water-extracted propolis. This is based on the presumption that major biofunctional components were fat-soluble, rather than water-soluble.