• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.481-486
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• 1995

The salt stress at seedling stage of winter barley was examined in different concentrations of NaCl containing 1/2 Hoagland solution. Fresh weight of seedling at 30 days after seeding was highest at 25mM of NaCl concentration containing 1/2 Hoagland solution but if the NaCl concentration was more than 50mM it began to decrease seriously. Water content in plant was decreased according to increase of NaCl concentration in 1/2 Hoagland solution, so physiological mechanism of NaCl in barley was different from saline plant. Stoma number per cm$^2$ of first leaf was higher than that of control in case of stressed by NaCl but in that case the leaf length was decreased so the number of stoma per first leaf was slightly decreased. Chloroplast shape was not changed by 75mM of high NaCl contained 1/2 Hoagland solution but cell division at root growing point was inhibited by 75mM of NaCl. As the result of salt stress mitochondria was ruined in structure and irregular solid was found to be transfered from the cytoplasm to the cell wall in root growing point.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.32-37
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• 1999

The experiment was conducted to investigate emergence response of lowland weeds at different soil moisture contents, burying depths and upon changes in soil moisture. Rice germination was over 50% at all burying depths under aerobic condition, but the emergence rate of the soil surface placed seeds in saturated and flooded conditions decreased by 19% and 29%, respectively, as compared with that of aerobic condition. Rice seeds at burying depth of over 3 cm did not emerge at all. The emergence rate of Echinochloa crus­galli (L.) Beauv. in aerobic condition was lower than 30%, but the emergence pattern of E. crus galli (L.) Beauv. at different soil moisture contents and seeding depths was similar to that of rice. Emergence behavior of lschaemum rugosum Salisb., Ludwigia octovalvis (Jacq.) Raven and Sphenoclea zeylanica Gaertn. which are dominant lowland weed species in the Philippines also differed depending on soil moisture conditions and burying depths. lschaemum rugosum Salisb. emerged at all burying depths under aerobic condition, whereasLudwigia octovalvis (Jacq.) Raven emerged only at 0 cm deep under saturated and aerobic condition and Sphenoclea zeylanica Gaertn. at 0 cm deep under flooding condition. Weed seeds planted at 1, 3, and 5 cm deep in continuous flooded and saturated condition did not emerge at all, but upon a change of soil moisture condition from saturated to drainage (S$\rightarrow$D) and flooded to drainage (F$\rightarrow$D), grass weeds began to germinate again and the average emergence rate in S$\rightarrow$D and F$\rightarrow$D were 26% and 5% forE. crus­galIi (L.) Beauv., 9% and 8% forI. rugosum SaIisb., respectively. Weed seeds buried in soil in the pot showed great emergence at S$\rightarrow$D but did not emerge under continuous flooded condition. The diversity index accounting for dominance degree and occurrence aspect of weed, was the lowest at F$\rightarrow$D.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.4
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• pp.64-73
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• 1999

This study was conducted to find out the effects of hydroseeding material and seed mixture on the revegetation of rock cut-slopes by PEC4 (Polymer-Ecology-Control) Hydroseeding Measures. PEC4 hydroseeding material was applied to four cut-slopes using hydroseeding measures from April to August, 1999, and the field survey was carried out by monthly. PEC4 material consisted of bark compost and organic soil amendments. This material has high content of organic matter and high level of water holding capacity. PEC4 hydroseeding material shows low level of soil hardness, so it gives to good condition for seed germinating and plant growing in early stage. PEC4 material attached at rock cut-slopes by two types of adhesive agent was not eroded by rainfall. The plant coverage and number of plant species were affected by mixing ratio of seeds and seeding timing. From the viewpoint of plant establishment, the optimal hydroseeding timing of mixed seeds for plant growth seems to be in May. Most of the plant seeds were germinated well and they covered rock cut-slopes so quickly and effectively. Plant importance value of Silene armeria and Platycodon grandiflorum. were higher than any other seeded-native species in the competition between native species and exotic species, so they have enough possibility to be used for slope revegetation works. Thus it leads to conclusion that the revegetation method used in this experiment was a very effective method for plant establishment on rock cut-slopes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.286-291
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• 2002

Seedling establishment of soybean [Glycine max (L.) Merr.] is a critical factor in production system and cultural practices. The objective of this study was to identify the components of soybean seedling developments encompassing planting dates and cultivars that respond to emergence, early growth stage and dry matter accumulation. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted at Baegsan silty loam soil. Planting date was May 13, June 3, and June 24 in 2001. Sprinkler irrigation was accompanied with 30mm after seeding for three planting dates. Soybean seedlings were sampled at the growth stages from VE to V5. Days to emergence of soybean seedlings were taken 8 at May 13 and 5 at June 24 plantings. Emergence percentage was over 90 at three planting dates. May 13 planting took 33 days and June 24 planting was 25 days for reaching V5 growth stage. Cotyledon number was decreased after V2. Significant cultivar difference was found in cotyledon dry weight until V2 which differed in seed dry weights at the planting times. Leaf and total dry weights of soybean seedlings were not differed from V1 to V3 among planting dates and cultivars. Leaf water contents were generally ranged 78 to 85%. Branch was appeared from V4. Leaf/stem ratio among cultivars was similar at five growth stages and gradually increased from 2.1 at V1 to 2.8 at V5. The results based on this experiment indicated that seedling establishment of soybean was continued from VE to V3 growth stages affecting mainly by planting date and soil moisture.

• Research in Plant Disease
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• v.15 no.2
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• pp.88-93
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• 2009

In 2008, leaf rot and blight on pepper seeding ("Dokya-chungchung") occurred in a pepper farm at Hwaseong-si, Gyeonggi-do, Korea. The typical symptom is water-soaking and dark brown leaf blight at edges and tips of leaves. The fungal colonies isolated from infected tissues were pinkish at first and turned gradually to gray. Conidia were fusiform, non-septum, and $8.1-17.0{\times}2.0-3.8{\mu}m$ in size. Several specific PCR primers derived from the sequence of the internal transcribed spacer (ITS) region of the rDNA, such as CaINT, CgINT and CcINT were used for the identification of the fungal pathogen. The C. acutatum-specific primer CaINT was amplified single fragment of 496 bp that discriminated C. acutatum from the other species. The pathogenicity test was performed on seedlings and fruits of red pepper. On the basis of the morphological, molecular characteristics and pathogenicity test, we identified as Colletotrichum acutatum. This is the first report on leaf rot and blight on pepper seedling caused by C. acutatum in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.93-101
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• 2008

The effects of $CO_2$ enrichment on growth of maize (Zea mays L.) were examined. Parameters analyzed include growth characteristics, yields, photosynthetic rates, evaporation rates and photosynthesis-related characteristics under elevated $CO_2$. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the silking stage. The plants were exposed to two elevated $CO_2$ of 500, 700ppm and ambient levels (350 ppm). Chalok 1 and GCB 70 germinated three days after seeding, and germination rates were faster in the elevated $CO_2$ than the control. Germination rates displayed significant differences among the $CO_2$ treatments. At the seedling stage, leaf area, top dry weight, and photosynthetic rates, and plant height indicated positive relationship with elevated $CO_2$ concentrations. At the $5{\sim}6$ leaf stage, $CO_2$ concentration also indicated positive relationship with plant height, leaf area, top dry weight, and photosynthetic rates. At the silking stage, increased plant height of Chalok 1 was noted in the $CO_2$ treatments compared to the control. No significant differences were noted for GCB 70, in which leaf area decreased but photosynthetic rates increased progressively with $CO_2$ concentration. Stomatal aperture was a little bigger in the elevated $CO_2$ than the control. $CO_2$ concentration was negatively related to stomatal conductance and transpiration rates, resulting in high water use efficiency.

• Korean journal of applied entomology
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• v.26 no.2 s.71
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• pp.107-111
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• 1987

Experiments were caried out to invesstigate the effects of seed disinfectant and the chemical control method in the paddy field for the rice white-tip nematode, Aphelenchoides besseyi. All three seed disinfectants tested, MEP 50% EC, Fenthion 50% EC, Dasuzin 34% EC showed effective control without any symptom of phytotoxicity. Mixed treatments with fungicides, Benoram 40% Wp, proraz-25% EC, TCM 30% EC also showed the effective control and no phytotoxicity. Effective chemical control methods for the rice white-tip nematode in paddy fields was seed disinfectant before seeding and Carbofuran 3% G. on the day before the transplanting, or seed disinfectatnt + Carbofuran 3% G. water surface treatment on the early stage of injury.

• Research in Plant Disease
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• v.9 no.1
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• pp.52-56
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• 2003

It was conducted to investigate the effect of rice downy mildew (RDM) infection to plant growth and yield components in water seeding stage, and to screen of varietal resistance to downy mildew. Being infected by rice downy mildew, chlorotic spot appeared in the leaf and leaf length was shortened. As the infected rice was growing, internode was not elongated properly and was deformed, and then panicle was not arised or mal-formed. Plant height of infected rice was shortened at all growth stage, and while the number of tillers of infected rice was more decreased than that of healthy plant before maximum tillering stage, and that of infected rice was more increased after heading stage. While the number of internode of infected tiller was much increased than that of healthy tiller internode length of infected tiller was shorter. As the rice infected by RDM severely, the number of panicles per square meter and ripening of rice was more decreased and yield of rice was extremely much decreased. As result of the varietal resistance screening with rice seedling, Geyh-wabyeo and Donjinbyeo were resistant varieties to downy mildew.

• Journal of Convergence for Information Technology
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• v.11 no.1
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• pp.128-135
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• 2021

The possibility and effectiveness of cryopreservation was determined to assess survival rates and improve stock management of thawed embryos of Manila clam, Ruditapes philippinarum. The ideal freezing rates were designed and tested to allow cryoprotectants to equilibrate across the membrane during freezing. Survival rates ranging from 0 to 64.3% were obtained using a stepwise freezing protocol compared with 82.3% control rates. Embryos of Ruditapes philippinarum were equilibrated in 2 CPAs plus sea water for 10 min at 25℃ and then cooled at -1℃/min from 20℃ to -12℃. Straws containing more than 100 embryos were held at 12℃ for 5 min allowing equilibration after seeding and slowly cooled at 2℃/min. to -35℃ for 30 min for equilibration before quenching in liquid nitrogen. Dimethyl sulfoxide (DMSO) is the best cryoprotectant indicated for embryos of R. philippinarum with a survival rate of 64.3±3.28% in the presence of 2.0 M DMSO.

• Journal of Practical Agriculture & Fisheries Research
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• v.24 no.3
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• pp.25-31
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• 2022

Low-temperature germinability (LTG) is an important trait for the direct seeding of rice in temperate and high altitudes regions. Water temperature of rice fields at sowing season is frequently under 15℃ in Korea, resulting in poor seedlings establishment. The objective of this study was to phenotypically and genetically evaluate 600 rice germplasm composed of six rice subpopulations. In the phenotypic evaluation, aus (AUS) showed the highest visual rating (VR) and germination rate (GR) of 5 and 83.1%, respectively, than other subpopulations. The lowest value of VR (7.1) and GR (53.1%) was observed in aromatic (ARO) subpopulation. Association of the phenotype for LTG with the genotype for qLTG3-1, a major QTL for LTG, and other three SNP markers (LTG_8, qLTG4b-1, qLTG2-6) was conducted. The results suggested that the allele distribution of two markers, qLTG3-1 and qLTG2-6, affects the difference in VR and GR between subpopulations. qLTG4b-1 and LTG_8 were also highly significant with LTG in all subpopulations except that qLTG4b-1 did now show significance in AUS. Therefore, the combination use of these four markers might be effective for evaluation of LTG in rice germplasm.