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Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain (인간태아의 뇌로부터 유래된 cDNA liberary에서 내생레트로바이러스 HERV-W pol 유전자의 동정과 계통)

  • Kim, Heui-Soo;Jeon, Seung-Heui;Yi, Joo-Mi;Kim, Tae-Hyung;Lee, Won-Ho
    • Journal of Life Science
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    • v.13 no.3
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    • pp.291-297
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    • 2003
  • A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.

Antioxidant Properties and Protective Effects of Inula britannica var. chinensis Regel on Oxidative Stress-induced Neuronal Cell Damage (금불초 추출물의 항산화 효과 및 산화 스트레스에 대한 신경세포 보호작용)

  • Lee, Na-Hyun;Hong, Jung-Il;Kim, Jin-Yung;Chiang, Mae-Hee
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.87-92
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    • 2009
  • The antioxidant properties and protective effects of Inula britannica on ${H_2}{O_2}$-induced SH-SY5Y neuroblastoma cell damage were investigated. A series of solvent fractions, including hexane(Fr.H), petroleum ether, chloroform, ethyl acetate(Fr.EA), and water fraction(Fr.W), were prepared from the 70% methanol extracts of Inula britannica. Fr.W had the highest total contents of phenolics and flavonoids, followed by Fr.EA. The antioxidant properties of the fractions were also evaluated by analyzing their scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and nitric oxide. Fr.W showed the strongest activities in all assays. The concentrations of Fr.W that resulted in 50% reductions of the DPPH and ABTS radicals were 20.7 ${\mu}g$/mL and 39.4 ${\mu}g$/mL, respectively. Fr.W showed the weakest cytotoxic activities on the SH-SY5Y cells, whereas it effectively protected ${H_2}{O_2}$-induced cell death, increasing cell survival by 35.0-77.0% at a concentration range of 62.5-250 ${\mu}g$/mL. In this range, Fr.W also significantly decreased intracellular ROS levels by 34-39%. Overall, the antioxidant properties of Inula britannica can contribute to rescuring neuronal cells from oxidative stress-induced cell injury.

Effect of plasmid curing on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W (글루탐산을 유일한 탄소 원과 질소 원으로 이용하는 Acinetobacter sp. B-W의 글루탐산으로부터의 시드로포어 생산에 미치는 플라스미드 제거 효과)

  • Kim, Kyoung-Ja;Lee, Jae-Rim;Yang, Yong-Joon
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.266-271
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    • 2018
  • Effect of plasmid curing of Acinetobacter sp. B-W on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources was investigated. Plasmid cured mutant of strain B-W lost the ability to produce siderophore from glutamic acid at $28^{\circ}C$. Transformant E. coli $DH5{\alpha}$ harboring 20 kb plasmid, that was isolated from wild type of strain B-W produced siderophore from glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$, but, not at $36^{\circ}C$. Production of siderophore from glutamic acid by transformant E. coli $DH5{\alpha}$ was completely inhibited by $10{\mu}M\;FeCl_3$. In previous report, catechol nature of siderophore produced from glutamic acid by strain B-W was detected by Arnow test. The siderophore produced from glutamic acid by transformant E. coli $DH5{\alpha}$ was also catechol type. Rf value of siderophore produced from transformant E. coli $DH5{\alpha}$ grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32 in butanol-acetic acid-water (12:3:5) as developing solvent. Rf value of the siderophore was the same with that of wild type of strain B-W. Thus a single plasmid of 20 kb seemed to be involved in the production of siderophore from glutamic acid.

Characteristics and classification of landform relieves on mountains and valleys with bedrock types (기반암별 산지와 곡지의 지형 기복 특성과 유형)

  • Lee, Gwang-Ryul
    • Journal of The Geomorphological Association of Korea
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    • v.21 no.4
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    • pp.1-17
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    • 2014
  • This study analyzed characteristics of landform relieves on 12 bedrock whole(W) areas and 24 mountain(M) and valley(V) areas. Based on this result, characteristics and relations between bedrocks and landform relief were classified as follows. 1) gneiss-height M and granite-height W, M, V areas show active stream incision for uplift. However these areas have relatively low relief and grade compared to high altitude, because effect of denudation don't pass on whole slope. 2) gneiss-height W, V, gneiss-mid M, schist M, granite-mid M, volcanic rock W, M, sedimentary rock-height(conglomerate) W, M, V, sedimentary rock-mid (sandstone and shale) M, limestone W, M areas have active stream erosion and mass movement, but landform relieves are on the high side, because these have resistant bedrock and geological structure against weathering and erosion. 3) gneiss-mid W, V, schist W, V, granite-mid W, V, volcanic rock V, sedimentary rock-mid W, V, sedimentary rock-low(shale) M, limestone V areas landform relieves are on the low side, because these have weak resistance and active weathering, mass movement, erosion, transportation and deposit. 4) gneiss-low W, M, V, granite-low W, M, V, sedimentary rock-low W, V areas landform relieves are very low, because these don't have active erosion and mass movement as costal area with low altitude.

A Study on the Emulsifying Stability of W/O Type Sunscreen Cream by the Hansen Solubility Parameter (Hansen Solubility Parameter 를 통한 W/O 형 자외선차단 제형의 유화 안정성에 관한 연구)

  • Kim, Dong Hee;Lee, Jin Jae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.4
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    • pp.273-280
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    • 2021
  • The water resistance is important factor for sunscreen formulations. Generally a sunscreen cream was formulated by a water-in-oil (W/O) emulsion. In the W/O emulsion system, silicone oils are added to improve the texture of formulations. Silicone oils have low compatibility with organic sunscreen agent, causing problems with the stability in emulsion. In this study, the compatibility between various oils in the W/O emulsion was derived numerically using Hansen solubility parameter (HSP) at first. HSP is represented a dispersion degree, a polarity, and a hydrgen bond in a composition. In this study, various emulsions were prepared according to the types of oils with different HSP values and then monitored by a viscosity and morphology according to the time and temperature. The HSP values of components and the experimental results have similar activities for the stability of emulsions. HSP made it easy to select oil with high compatibility. When the compatibility of the oil phase in the W/O emulsion was high, the viscosity change over time was small. The stability was improved under the freeze-thaw cycle (-15 ℃ ~ 45 ℃). In the future, if the composition of the ingredients is optimized through HSP, it is expected that it will be helpful in the development of W/O type sunscreen formulations that are excellent in use and stability.

Probability Models of W-CDMA Signals in Realistic Wideband Multipath Channels (광대역 다중경로 실측채널에서 W-CDMA 수신 신호의 화률 모델)

  • 오동진;이주석;이귀상;김철성
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.4B
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    • pp.308-315
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    • 2002
  • This paper proposes new probability models for wideband code division multiple access (W-CDMA) signals. The performance of a W-CDMA system is evaluated by calculating the average bit error rate(BER) which is derived from the probability distribution of the W-CDMA receiver output. If a probability model of the receiver output is available, the performance evaluation becomes much simpler and it enables diverse analyses of the system for channel coding and other purposes. In this paper, probability distributions of W-CDMA signals, more specifically those of the receiver output, are represented as Rayleigh and noncentral chi distribution, considering various bandwidths and channel environments. The adequacy of a probability model is verified by chi-square test of 1% significance level. The BER of the system obtained from the simulation results is compared to that obtained from the probability model to demonstrate the usefulness of the proposed models.

PHOTOELECTRIC OBSERVATIONS AND ANALYSIS OF THE CONTACT BINARY W UMA (접촉쌍성 W UMa의 광전관측과 광도곡선 분석)

  • 김천휘;이충욱;강영운
    • Journal of Astronomy and Space Sciences
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    • v.18 no.1
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    • pp.43-54
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    • 2001
  • The unpublished photoelectric observations of the contact binary W UMa in ultraviolet, blue, and yellow bandpasses made on four nights during the obsrving seasons from April, 1984 to May, 1985 were presented. From our observations new eleven times of minimum light were determined. By using the 1993 WD binary model our light curves were analyzed and new photometric solutions of W UMa were obtained.

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ℂ-VALUED FREE PROBABILITY ON A GRAPH VON NEUMANN ALGEBRA

  • Cho, Il-Woo
    • Journal of the Korean Mathematical Society
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    • v.47 no.3
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    • pp.601-631
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    • 2010
  • In [6] and [7], we introduced graph von Neumann algebras which are the (groupoid) crossed product algebras of von Neumann algebras and graph groupoids via groupoid actions. We showed that such crossed product algebras have the graph-depending amalgamated reduced free probabilistic properties. In this paper, we will consider a scalar-valued $W^*$-probability on a given graph von Neumann algebra. We show that a diagonal graph $W^*$-probability space (as a scalar-valued $W^*$-probability space) and a graph W¤-probability space (as an amalgamated $W^*$-probability space) are compatible. By this compatibility, we can find the relation between amalgamated free distributions and scalar-valued free distributions on a graph von Neumann algebra. Under this compatibility, we observe the scalar-valued freeness on a graph von Neumann algebra.

Three Species of the Genus Walckenaeria (Araneae: Linyphiidae) New to Korea (코뿔애접시거미속 (거미목: 접시거미과)의 3 한국미기록종)

  • Seo, Bo Keun
    • Korean journal of applied entomology
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    • v.52 no.2
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    • pp.101-108
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    • 2013
  • Three species of the linyphiid genus Walckenaeria, Walckenaeria chikunii Saito and Ono, 2001, Walckenaeria furcillata (Menge, 1869), and Walckenaeria ichifusaensis Saito and Ono, 2001, are reported new to the Korean spider fauna. W. furcillata is widely distributed throughout the Palearctic region, but W. chikunii and W. ichifusaensis are only known from their type localities in Japan. Photographs and illustrations of the diagnostic characters are provided.

객체지향설계법과 구조설계 전산화

  • 김치경
    • Computational Structural Engineering
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    • v.6 no.2
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    • pp.5-12
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    • 1993
  • 본 고에서는 객체지향설계 및 프로그래밍 기법의 기본적인 개념과 구조설계 전산화에 관한 원론적인 사항에 대하여 고찰하여 보았다. OODP는 이미 S/W 개발분야에서 일반화된 기법이며, 한 때 큰 관심을 모으다 결국 실용화에 실패한 인공지능 또는 전문가시스템고는 달리, S/W 개발 및 유지관리에 큰 발전을 가져오고 있다. 객체 단위에 의한 데이타 및 함수의 통합모듈화, 추상화 및 정보은닉에 의한 객체의 독립성 확보, 객체지향 프로그래밍 언어의 탁월한 이식성, 객체추가에 의한 대규모 S/W의 점진적 개발, 상송성 및 다형성에 의한 프로그램 코드의 재사용성 등은 S/W의 부품화를 가능케 하고 S/W 신뢰도를 크게 높혀 주고 있다. OODP는 구조설계 전산화에도 적합한 기법으로 판단된다. OODP 기법은 전산전문가들의 도구가 아니며 각 분야 전문가들이 자기 분야의 전산화를 위해 사용하는 도구이다. 과거 우리가 fortran을 익혀 구조해석용 S/W를 개발했듯이 이제는 OODP 기법을 익혀 적극적으로 우리 문제에 활용한다면, 우리 분야 전산화 수준을 높히는데 크게 도움이 될 것으로 기대된다.

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