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http://dx.doi.org/10.5352/JLS.2003.13.3.291

Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain  

Kim, Heui-Soo (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Jeon, Seung-Heui (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Yi, Joo-Mi (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Kim, Tae-Hyung (Interdisciplinary Program of Bioinformatics, Pusan National University)
Lee, Won-Ho (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.13, no.3, 2003 , pp. 291-297 More about this Journal
Abstract
A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.
Keywords
cDNA library; Fetal brain; HERV-W; pol fragments; Phylogeny;
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1 Sugimoto, J., N. Matsuura, Y. Kinjo, N. Takasu, T. Oda and Y. Jinno. 2001. Transcriptionally active HERV-K genes: identification, isolation, and chromo-somal mapping. Genomics 72, 137-144   DOI   ScienceOn
2 Sverdlov, E. D. 1998. Perpetually mobile footprints of ancient infections in the human genome. FEBS Lett. 428, 1-6   DOI   ScienceOn
3 Varmus, H. E. 1982. Form and function of retroviral proviruses. Science 216, 812-820   DOI
4 Voisset, C., A Blancher, H. Perron, B. Mandrand, F. Mallet and G. Paranhos-Baccala. 1999. Phylogeny of a novel family of human endogenous retrovirus sequences, HERV-W, in humans and other primates. AIDS Res. Hum. Retroviruses 15, 1529-1533   DOI   ScienceOn
5 Voisset, C., O. Bouton, F. Bedin, L. Duret, B. Man-drand, F. Mallet and G. Paranhos-Baccala. 2000. Chromosomal distribution and coding capacity of the human endogenous retrovirus HERV-W family. AIDS Res. Hum. Retroviruses 16, 731-740   DOI   ScienceOn
6 Wimmer, R., S. Kirsch, G. A. Rappold and W. Schempp. 2002. Direct evidence for the Homo-Pan clade. Chromo Res. 10, 55-61   DOI   ScienceOn
7 Yi, J. M., H. M. Kim and H. S. Kim. 2001. Molecular cloning and phylogenetic analysis of the human endogenous retrovirus HERV-K LTR elements in various cancer cells. Mol. Cells 12, 137-141
8 Yoder, J. A., C. O. Walsh and T. H. Bestor. 1997. Cytosine methylation and the ecology of intrage-nomic parasites. Trends Genet. 13, 335-340   DOI   ScienceOn
9 Kim, H. S., H. Hirai and O. Takenaka. 1996. Molecular features of the TSPY gene of gibbons and Old World monkeys. Chrom Res. 4, 500-506   DOI   ScienceOn
10 Kim, H. S., B. H. Hyun, J. Y. Choi and T. J. Crow. 2000a. Phylogenetic analysis of a retroposon family as represented on the human X chromosome. Gene Genet. Syst. 75, 197-202   DOI   ScienceOn
11 Kim, H. S., B. H. Hyun and T. J. Crow. 2000b. Phylogenetic analysis of retroposon family as exem-plified on human chromosome 13: further evidence for recent proliferation. Mol. Cells 10, 356-360
12 Kim, H. S. and W. H. Lee. 2001. Human endogenous retrovirus HERV-W family: chromosomal localization, identification, and phylogeny. AIDS Res. Hum. Retro-viruses 17, 643-648   DOI   ScienceOn
13 Kim, H. S., O. Takenaka and T. J. Crow. 1999. Isolation and phylogeny of endogenous retrovirus sequences belonging to the HERV-W family in pri-mates. J. Gen. Viral. 80, 2613-2619
14 Kumar,S., K. Tamura and N. Nei. 1993. MEGA: Molecular evolutionary genetics analysis, version 1.01. The Pennsylvania State University, University Park, PA 16802
15 Blond, J. L., F. Beseme, L. Duret, O. Bouton, F. Bedin, H. Perron, B. Mandran and F. Mallet. 1999. Molecular characterization and placental expression of HERVW, a new human endogenous retrovirus family. J. Virol. 73, 1175-1185
16 Leib-Mosch, C., M. Bachmann, R. Brack-Werner, T. Werner, V. Erfle and R. Hehlmann. 1992. Expression and biological significance of human endogenous retroviral sequences. Leukemia 6, 72S-75S
17 Perron, H., J. A Garson, F. Bedin, F. Beseme, G. Paranhos-Baccala, F. Komurian-Pradel, F. Mallet, P. W. Tuke, C. Voisset, J. L. Blond, B. Lalande, J, M. Seigneurin, B. Mandrand. and the collaborative re-search group on MS (1997) Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis. Proc. Natl. Acad. Sci. USA 94, 7583-7588   DOI
18 Schon, U., W. Seifarth, C. Baust, C. Hohenadl, V. Erfle and C. Leib-Mosch. 2001. Cell type-specific expression and promoter activity of human endogenous retroviral long terminal repeats. Virology 279, 280-291   DOI   ScienceOn
19 Deb-Rinker, P., T. A. Klernpan, R. L. O'Reilly, E. F. Torrey and S. M. Singh. 1999. Molecular charac-terization of a MSRV-like sequence identified by RDA from monozygotic twin pairs discordant for schizophrenia. Genomics 61, 133-144   DOI   ScienceOn
20 Garson, J. A., P. W. Tuke, P. Giraud, G. Paranhos-Baccaia and P. Perron. 1998. Detection of virione-associated MSRV-RNA in serum of patients with multiple sclerosis. Lancet 351, 33-33   DOI   ScienceOn
21 Kazazian, H. H and J. V. Moran. 1998. The impact of the LI retrotransposons on the human genome. Nature Genet. 19, 19-24   DOI   ScienceOn
22 Karlsson, H., S. Bachmann, J. Schroder, J. AcArthur, E. F. Torrey and R. H. Yolken. 2001. Retroviral RNA identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Proc. Natl. Acad. Sci. USA 98, 4634-4639   DOI   ScienceOn
23 Kim, H. S. and T. J. Crow. 2000. Phylogenetic rela-tionships of a class of hominoid-specific retro-ele-ments (SINE-R) on human chromosome 7 and 17. Ann. Hum. Biol. 27, 83-93   DOI   ScienceOn
24 Kim, H. S. 2001. Isolation and phylogeny of HERV-W pol fragments. AIDS Res. Hum. Retroviruses 17, 1665-1671   DOI   ScienceOn
25 Kim, H. S., Y. Chen and P. Lonai. 1998. Complex regulation of multiple cytohesin like genes in murine tissues and cells. FEBS Lett. 433, 312-316   DOI   ScienceOn
26 Kim, H. S. and T. J. Crow. 1999. Presence and phy-logenetic analysis of HERV-K LTR on human X and Y chromosomes: evidence for recent proliferation. Genes Genet. Syst. 74, 267-270   DOI   ScienceOn
27 Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller and J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402   DOI   ScienceOn