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http://dx.doi.org/10.7845/kjm.2018.8026

Effect of plasmid curing on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W  

Kim, Kyoung-Ja (Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Lee, Jae-Rim (Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
Publication Information
Korean Journal of Microbiology / v.54, no.3, 2018 , pp. 266-271 More about this Journal
Abstract
Effect of plasmid curing of Acinetobacter sp. B-W on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources was investigated. Plasmid cured mutant of strain B-W lost the ability to produce siderophore from glutamic acid at $28^{\circ}C$. Transformant E. coli $DH5{\alpha}$ harboring 20 kb plasmid, that was isolated from wild type of strain B-W produced siderophore from glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$, but, not at $36^{\circ}C$. Production of siderophore from glutamic acid by transformant E. coli $DH5{\alpha}$ was completely inhibited by $10{\mu}M\;FeCl_3$. In previous report, catechol nature of siderophore produced from glutamic acid by strain B-W was detected by Arnow test. The siderophore produced from glutamic acid by transformant E. coli $DH5{\alpha}$ was also catechol type. Rf value of siderophore produced from transformant E. coli $DH5{\alpha}$ grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32 in butanol-acetic acid-water (12:3:5) as developing solvent. Rf value of the siderophore was the same with that of wild type of strain B-W. Thus a single plasmid of 20 kb seemed to be involved in the production of siderophore from glutamic acid.
Keywords
Acinetobacter sp. B-W; L-glutamic acid; plasmid curing; siderophore; sole carbon and nitrogen sources;
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