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Antioxidant and Immune-Modulating Activities of Egg Yolk Protein Extracts

  • Lee, Jae Hoon;Lee, Yunjung;Paik, Hyun-Dong;Park, Eunju
    • Food Science of Animal Resources
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    • v.42 no.2
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    • pp.321-331
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    • 2022
  • Egg yolk is widely used to extract lecithin, which is utilized in the food and cosmetics industry. After lecithin is removed, the rest of egg yolk is generated as a by-product. Thus, it is necessary to properly utilize it. In this study, egg yolk protein extracts were produced using ethanol (EYE-E) and water (EYE-W). Their antioxidant and immunomodulatory effects were then evaluated. Antioxidant activities of EYE-E and EYE-W were determined using cellular antioxidant capacity (CAC) assay and comet assay. EYE-E and EYE-W showed significant (p<0.05) scavenging effects on intracellular reactive oxygen species (ROS) in a dose dependent manner. At a concentration of 50 ㎍/mL, EYE-W showed higher (p<0.05) antioxidant activity than EYE-E. EYE-E and EYE-W also exhibited protective effects against DNA damage caused by oxidative stress. After treatment with EYE-E and EYE-W, DNA damage level of 48.7% due to oxidative stress was decreased to 36.2% and 31.8% levels, respectively. In addition, EYE-E and EYE-W showed immunomodulatory effects by regulating Th1 cytokines (TNF-α and IL-2) and Th2 cytokines (IL-10 and IL-4) in Balb/c mouse splenocytes. These data suggest that EYE-E and EYE-W could be used as functional food ingredients with excellent antioxidant and immunomodulatory activities in the food industry.

Fabrication of AC4A/$SiC_w$composite by squeeze casting(II) (용탕단조법에 의한 AC4A/$SiC_w$복합재교 제조에 관한 연구(ll)-가압력 및 시효특성-)

  • Mun, Gyeong-Cheol;Lee, Chun-Hui
    • Korean Journal of Materials Research
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    • v.3 no.6
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    • pp.606-613
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    • 1993
  • This was studied about aging characteristic of AC4A/$SiC_{w}$ 10-30v/o reinforced composite. Aging hardenability was decreased $SiC_{w}$ 30% > 10% > 20%. Aging hardening of T6 treatmented composite was higher absolute value than AC4A I/M material. And this results indicated initial hardening phenomenon according to increase $SiC_{w}$ volume fraction. Reinforced effect by pressure was the same effect as before aging treatment and the best condition pressure at 75MPa. Similar to reinforced effect according to $SiC_{w}$ volume fraction was 30 % > 10 % > 20 %. In case of pressure is low, whisker is not break the same time press with base metal after wetting. After it is wetting with base metal, a part transformed or wetting part break and whisker maintain original shape or a part transformed on the otherhand, in case of pressure is high, whisker is break in same time it was not against pressure and whisker's shape is near a polygon or spherical shape.

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Optimization of Medium Composition for the Mycelial Growth of Sparassis crispa (꽃송이버섯의 균사 생장을 위한 배지 조건 최적화)

  • Kim, Jin-Woo;Cheon, Woo-Jae;Chai, Kyung-Hee;Kim, Dong-Gwan;Son, Sung-Ho;Kim, Jong-Guk;Lim, Hee-Jae
    • Journal of Life Science
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    • v.22 no.2
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    • pp.200-208
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    • 2012
  • The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, and 0.1% (w/v) folic acid].

Quality characteristics of sausage added red yeast rice (홍국쌀 첨가 소시지의 품질 특성)

  • Kim, Do-Wan
    • Food Science and Preservation
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    • v.20 no.6
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    • pp.805-809
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    • 2013
  • This study was carried out to investigate the effects and characteristics of sausages with red yeast rice added, particularly the Monascus purpureus KFRI 1134 strains, and to decide the optimal quantity of red yeast rice used. The additional rate of red yeast rice which has been used as a material for reducing nitrite, a coupler in meat products were 0, 1, 3, and 5% (w/w). In terms of the effects of the red yeast rice on the color, texture properties, and sensory characteristics of the sausages, the optimal temperature at which color stability was shown was below $100^{\circ}C$, and the addition of 1% red yeast rice resulted in a distinctly attractive color and increasingly improved the red color in the UV-visual Spectrophotometer analysis. However, the addition of over 1% (w/w) resulted in an unattractive color. The red colors also had strong heat stability. The texture properties and taste of the sausages were not affected by the red yeast rice.

Prokaryotic Selectivity, Anti-endotoxic Activity and Protease Stability of Diastereomeric and Enantiomeric Analogs of Human Antimicrobial Peptide LL-37

  • Nan, Yong-Hai;Lee, Bong-Ju;Shin, Song-Yub
    • Bulletin of the Korean Chemical Society
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    • v.33 no.9
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    • pp.2883-2889
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    • 2012
  • LL-37 is the only antimicrobial peptide (AMP) of the human cathelicidin family. In addition to potent antimicrobial activity, LL-37 is known to have the potential to inhibit lipolysaccharide (LPS)-induced endotoxic effects. To provide the stability to proteolytic digestion and increase prokaryotic selectivity and/or anti-endotoxic activity of two Lys/Trp-substituted 19-meric antimicrobial peptides (a4-W1 and a4-W2) designed from IG-19 (residues 13-31 of LL-37), we synthesized the diastereomeric peptides (a4-W1-D and a4-W2-D) with D-amino acid substitution at positions 3, 7, 10, 13 and 17 of a4-W1 and a4-W2, respectively and the enantiomeric peptides (a4-W1-E and a4-W2-E) composed D-amino acids. The diastereomeric peptides exhibited the best prokaryotic selectivity and effective protease stability, but no or less anti-endotoxic activity. In contrast, the enantiomeric peptides had not only prokaryotic selectivity and anti-endotoxic activity but also protease stability. Our results suggest that the hydrophobicity and ${\alpha}$-helicity of the peptide is important for anti-endotoxic activity. In particular, the enantiomeric peptides showed potent anti-endotoxic and LPS-neutralizing activities comparable to that of LL-37. Taken together, both a4-W1-E and a4-W2-E holds promise as a template for the development of peptide antibiotics for the treatment of endotoxic shock and sepsis.

Implementation of Multilateral Control System for Small UAV Control-Focused on Design (소형 무인기 통제를 위한 다자간 방식 관제시스템 구축방안-설계 중심으로)

  • Choi, Hyun-Taek;Kim, Seok-Kwan;Ryu, Gab-Sang
    • Smart Media Journal
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    • v.6 no.4
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    • pp.65-71
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    • 2017
  • In this paper, we propose a design method for the construction of LTE-based small unmanned aerial vehicle control system to quickly and reliably collect multiple small unmanned aerial vehicle position information simultaneously flying all over the country. In particular, the main requirements are the network (N/W), hardware (H/ W), software(SW), Database(DB), development architecture, and business needs. To satisfy these requirements, N/W, H/W, SW, DB design, and architectural design plan were suggested regarding the design requirements of a small UAV system. To effectively control the small unmanned multi-party system in the system design, the architecture is divided into the front-end service area and the back-end service area according to the function and role of the unit system. In the front-end service area that grasps and controls the position and state of small unmanned aerial vehicles (UAVs), we have studied the design part that can be expanded to N through TCP/IP network by applying Client PC method.

Characteristics of Galactooligosaccharide Production Using Cellulases (셀룰라제를 이용한 갈락코올리고당의 생산 특성)

  • 신현재;양지원
    • KSBB Journal
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    • v.11 no.3
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    • pp.317-322
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    • 1996
  • Galactooligosaccharlde (GOS) is a kind of functional oligosaccharides that can be used as a food ingredient and a cosmetic additive. In this paper, characteristics of GOS synthesis by cellulase, using lactose as a substrate, were investigated. Penicillium funiculosum cellulose was found to be the most efficient for GOS production among six cellulose tested. The optimum pH and temperature for GOS production were 5.0 and $50^{\circ}C$, respectively. There was an optimum ratio of lactose concentration to enzyme loading; the value was 10 (w/w). The reaction pattern of P. funiculosum cellulase is consistent with that of microbial ${\beta}$-galactosidase which shows transgalactosylation activity. Amounts of GOS produced from 20% (w/v) lactose after 6 h incubation at $50^{\circ}C$, were 23% (w/w) based on total saccharide in the reaction medium. The GOS % increased with initial lactose concentration in the range of 5 to 20%. The products mainly consisted of a trisaccharide and tetrasaccharide from HPLC and TLC analysis. Among enzymes involved in transgalactosylation reaction, high molecular weight fractions over 50,000 Da, presumably ${\beta}$-glucosldase, were considered to be responsible for GOS production. Using this cellulose, a direct synthesis of galactosyl g1ucoside including GOS could be readily achieved with lactose as a galactosyl donor.

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A Study on Horizontal Ground Source Heat Pump Systems (수평형 지열원 히트펌프 시스템에 관한 연구)

  • Park, Yong-Jung;Kim, Kyoung-Hoon
    • Journal of Energy Engineering
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    • v.15 no.3 s.47
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    • pp.160-165
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    • 2006
  • Ground source heat pump (GSHP) or geothermal heat pump systems (GHPs) are recognized to be outstanding heating and cooling systems. Most of GSHP systems installed and studied in korea are vertical GSHP systems. A horizontal GSHP system was installed in greenhouse and investigated for the performance characteristics. The results of the study showed that the heating coefficient of performance of the heat pump was 3.64 and the overall heating coefficient of performance of the system was 3.31. The pumping power was obtained as 28.0 W/kW and the required ground heat exchanger length was 53.3 m/kW of rejection heat of condenser. The heat extraction rate was, on average, 14.58 W/m of ground heat exchanger length and trench length is 27.7 m/kW of rejection heat of condenser.

Studies on the Low-temperature Storage of Strawberry Pulp and Red Pepper Paste by Cryoprotectants (동결방지제에 의한 딸기펄프와 붉은생고추 페이스트의 저온저장에 관한 연구)

  • Jeong, Jin-Woong;Jo, Jin-Ho;Kwon, Dong-Jin;Kim, Young-Boong
    • Korean Journal of Food Science and Technology
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    • v.22 no.4
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    • pp.434-438
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    • 1990
  • With adding cryoprotectants to red pepper paste and strawberry pulp, the effects of freezing point depression and the energy requirement for storage at $-15^{\circ}C$ were studied. In case of red pepper paste, the freezing point was pressed to $-9.6^{\circ}C$ by adding NaCl(15% w/w) and citric acid after removing stem. And the components of combined cryoprotectants to keep strawberry pulp in the unforzen state at $-15^{\circ}C$ were dextrose(25% w/'w), fructose(17% w/w) sorbitol(8% w/w) and ascorbic acid(0.2%, w/w). Also, when compared with No-treatment, the storage time reduced about 50% and resulted in about $70{\sim}80%$ cut-down in the energy requirement for storage of food products at low-temperature.

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Characteristics of W-TiN Gate Electrode Depending on the Formation of TiN Thin Film (W-TiN 복층 전극 소자에서 TiN 박막 형성 조건에 따른 특성 분석)

  • 윤선필;노관종;양성우;노용한;김기수;장영철;이내응
    • Journal of the Korean Vacuum Society
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    • v.10 no.2
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    • pp.189-193
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    • 2001
  • We have characterized physical and electrical properties of W-TiN stacked gate electrode structure with TiN as a diffusion barrier of fluorine. As the $N_2/Ar$ gas ratio increased during sputter deposition, TiN thin films became N-rich, and the resistivity of the films increased. However, the resistivity of W-TiN stacked gate reduced as a result of the crystallization of tungsten with the increase of $N_2/Ar$ gas ratio. On the other hand, tungsten in W-TiN stacked gate structure have the (100)-oriented crystalline structure although TiN films were subjected to annealing at high temperature (600~$800^{\circ}C$). Leakage currents of W-TiN gate MOS capacitors were less than $10^{-7}\textrm{/Acm}^2$ and also were lowered by the order of 2 compared with those of pure W gate electrode.

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