• Journal of Microbiology and Biotechnology
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• v.1 no.2
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• pp.130-135
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• 1991

The effects of media composition on citric acid fermentation using surface immobilized Saccharomycopsis lipolytica were studied. The use of the standard medium for these organisms resulted in rapid decrease of citric acid production and a transformation of immobilized cell morphologies from a yeast-type to a mycelium-type. When the standard medium was enriched with vitamins, trace minerals, a growth factor and ammonium to form a Vigorous Stationary Phase (VSP) fermentation type medium, relatively stable citric acid production (10 mg/lㆍh) was obtained. Using the VSP type medium, the surface immobilized cells also retained their yeast-type form.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.28 no.1
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• pp.59-65
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• 2020

This paper presents comparative results of configuration modeling and aerodynamic analysis to single-engine airplanes such as C-172, SR-20, and DA40NG. The software OpenVSP was used as an airplane configuration modeling tool. OpenVSP can provide the fastest method to get three-dimensional aircraft configuration from given basic data and drawings of aircraft. Parametric design input in OpenVSP, from given aircraft geometric parameters, was applied to small airplanes mentioned. New aircraft models in this study were reversely designed to coincide with the publicly obtained dimensions of the original aircraft. The basic aerodynamic analysis of newly designed modeling aircraft was performed by the vortex lattice method. Results are shown that the similarity of aerodynamic data obtained except for the lack of DA40NG. In conclusion, the modeling process applied to this work is valuable to obtain conceptual design insight in the reverse design from the small airplanes currently in use for flight training.

• Molecules and Cells
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• v.24 no.2
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• pp.185-193
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• 2007

Symbiotic nitrogen fixation with nitrogen-fixing bacteria in the root nodules is a distinctly beneficial metabolic process in legume plants. Legumes control the nodule number and nodulation zone through a systemic negative regulatory system between shoot and root. Mutation in the soybean NTS gene encoding GmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule development called hypernodulation. To examine the effect of nts mutation on the gene expression profile in the leaves, suppression subtractive hybridization was performed with the trifoliate leaves of nts mutant 'SS2-2' and the wild-type (WT) parent 'Sinpaldalkong2', and 75 EST clones that were highly expressed in the leaves of the SS2-2 mutant were identified. Interestingly, the expression of jasmonate (JA)-responsive genes such as vspA, vspB, and Lox2 were upregulated, whereas that of a salicylate-responsive gene PR1a was suppressed in the SS2-2 mutant. In addition, the level of JA was about two-fold higher in the leaves of the SS2-2 mutant than in those of the WT under natural growth conditions. Moreover, the JA-responsive gene expression persists in the leaves of SS2-2 mutant without rhizobia infection in the roots. Taken together, our results suggest that the nts mutation increases JA synthesis in mature leaves and consequently leads to constitutive expression of JA-responsive genes which is irrelevant to hypernodulation in the root.

• Food Science of Animal Resources
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• v.38 no.2
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• pp.338-349
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• 2018

The objective of this study was to investigate comparison of physicochemical, microbiological, and sensory characteristics of Hanwoo eye of round by various packaging methods [wrapped packaging (WP), modified atmosphere packaging (MAP), vacuum packaging (VP) with three different vacuum films, and vacuum skin packaging (VSP)] at a small scale. Packaged Hanwoo beef samples were stored in refrigerated conditions ($4{\pm}1^{\circ}C$) for 28 days. Packaged beef was sampled on days 0, 7, 14, 21, and 28. Physicochemical [pH, surface color, thiobarbituric acid reactive substances (TBARS), and volatile basic nitrogen (VBN) values], microbiological, and sensory analysis of packaged beef samples were performed. VP and VSP samples showed low TBARS and VBN values, and pH and surface color did not change substantially during the 28-day period. For VSP, total viable bacteria, psychrotrophic bacteria, lactic acid bacteria, and coliform counts were lower than those for other packaging systems. Salmonella spp. and Escherichia coli O157:H7 were not detected in any packaged beef samples. A sensory analysis showed that the scores for appearance, flavor, color, and overall acceptability did not change significantly until day 7. In total, VSP was effective with respect to significantly higher $a^*$ values, physicochemical stability, and microbial safety in Hanwoo packaging (p<0.05).

• Food Science of Animal Resources
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• v.21 no.3
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• pp.235-245
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• 2001

This study was conducted to evaluate the usefulness of Second-Heat-Seal film(SHS) as an alternative material to PVDC/EVA shrink film(VSP) being currently used by domestic meat packer for vacuum-packaging of fresh meat. The samples from pork loin and beef striploin and round were stored at 2$^{circ}C$ for 5 weeks and measured for the changes of microbial counts, color, pH, volatile basic nitrogen(VBN), purge loss and sensory parameters. The pork loins packed with SHS showed higher spermine contents during the whole storage period at 2$^{circ}C$, and lower counts in total microbes and lactic acid bacteria after 28 days storage at 2$^{circ}C$ than those packed with SHS tended to be lower than those packed with VSP over the storage time. Nevertheless, no significant differences were observed between two packaging treatments in the other quality parameters evaluated. It is therefore concluded that SHS film might have a possibility to substitute for VSP film for vacuum-packaging of fresh meat at least from a materials point of view.

• Journal of the Korean Geophysical Society
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• v.5 no.2
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• pp.75-86
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• 2002

A TSP(Tunnel Seismic Prediction) survey which is modified VSP(Vertical Seismic Profiling) survey applied in tunnel was carried out at Pyongtaek and Incheon liquefied petroleum gas(LPG) storage cavern during excavation. The TSP survey in Pyongtaek LPG storage cavern which is located below Namyangho was performed to confirm the location and orientation of the fault detected at pre-investigation stage. The TSP survey was carried out in access tunnel, construction tunnel, and watercurtain tunnel to characterize 3 dimensional figure of the fault. The results of TSP survey are compared four in vestigation boreholes drilled in shelter of access tunnel. The fault was also detected by borehole survey and the location was coincided with the result of TSP survey. Depending on the result of TSP survey and core logging, the design such as cavern layout and length was changed. Another TSP survey was performed in Incheon LPG storage cavern which is located below sea. Because of poor geological information at pre-investigation stage and suffering from heavy leakage of groundwater, the TSP survey to detect fracture zone was carried out. The support and grouting design was reflected by the result of TSP survey.

• Journal of Korean Institute of Industrial Engineers
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• v.14 no.1
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• pp.119-131
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• 1988

Vehicle Scheduling Problem (VSP) is a generic name given to a whole class of problems involving the visiting of "stations" by "vehicles," where a time is associated with each activity. The studies performed to date have the common feature of a single objective while satisfying a set of restrictions and known customer supplies or demands. However, VSPs may involve relevant multiple objectives and probabilistic supplies or demands at stations, creating multicriteria stochastic VSPs. This paper proposes a heuristic algorithm based on goal programming approach to schedule the most satisfactory vehicle routes of a bicriteria VSP with probabilistic supplies at stations. The two relevant objectives are the minimization of the expected travel distance of vehicles and the minimization of the due time violation for collection service at stations by vehicles. The algorithm developed consists of three major stages. In the first stage, an artificial capacity of vehicle is determined, on the basis of decision maker's subjective estimates. The second one clusters a set of stations into subsets by applying an efficient cluster method developed. In the third one, the stations in each subset are scheduled by applying an iterative goal programming heuristic procedure to each cluster.

• Journal of Industrial Technology
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• v.35
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• pp.15-21
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• 2015

In this paper, we implemented a high performance automated external defibrillator(AED) simulator that has various characteristics of virtual simulated patient(VSP) to verify important operations and functions of the AED. To verify the AED in various environment, the AED simulator should have the characteristics of VSP and can analyze the characteristics of electrical shock rhythm. In this paper, the implemented simulator has twenty seven electrocardiogram scenarios and we showed the implemented simulator has less error rate than conventional simulator in analyzing electrical shock rhythm.

• Tunnel and Underground Space
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• v.5 no.3
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• pp.214-222
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• 1995

Geological events which are undetected by the surface geophysical or geological survey phase can cause many problems, especially when the tunnel is excavated by TBM. To detect the geological events ahead of tunnel face, a seismic method applied from VSP method is used. Generally uniaxial geophone has been used in surface seismic survey. But this time, triaxial geophone is used to reduce the noise of tunnel wave. DME(Dip moveout Enhancement) filter and diffraction stack method are used. Applying these techniques to the road tunnel in construction, it is proved that the geological events ahead of tunnel face is fairly well predicted. From the seismic trace, Vp and Vs which are related to the rock property can be also obtained. Rock property and proper support design can be dedced from these parameters.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.36 no.4
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• pp.161-167
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• 2014

Purpose: The reconstruction of mandibular defects poses many difficulties due to the unique, complex shape of the mandible and the temporomandibular joints. With development of microvascular anastomosis, free tissue transplantation techniques, such as deep circumflex iliac artery (DCIA) flap and fibular free flap (FFF), were developed. The DCIA offers good quality and quantity of bone tissue for mandibular segmental defect and implant for dental rehabilitation. Virtual surgical planning (VSP) and stereolithography-guided osteotomy are currently successfully applied in three-dimensional mandibular reconstruction, but most use FFF. There are only a few articles on reconstruction with the DCIA that assess the postoperative results. Methods: Three patients admitted during a five month period (April of 2013 to August of 2013) underwent resection of mandible and DCIA musculo-osseous reconstruction using a VSP and stereolithographic modeling and assessment of outcomes included technical accuracy, esthetic contour, and functional outcomes. Results: This technique yielded iliac bone segment with excellent apposition and duplication of the preoperative plan. Flap survival was 100 percent and all patients maintained preoperative occlusion and contour. Conclusion: Based on our experience, we offer considerations and logically consistent protocols by classification of mandibular defects, and demonstrate the benefits in VSP and stereolithographic modeling of mandibular reconstructive surgery with DCIA flap.