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The Hypernodulating nts Mutation Induces Jasmonate Synthetic Pathway in Soybean Leaves  

Seo, Hak Soo (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Li, Jinjie (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Lee, Sun-Young (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Yu, Jae-Woong (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Kim, Kil-Hyun (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Lee, Suk-Ha (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Lee, In-Jung (Department of Applied Biosciences, Kyungpook National University)
Paek, Nam-Chon (Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University)
Publication Information
Molecules and Cells / v.24, no.2, 2007 , pp. 185-193 More about this Journal
Abstract
Symbiotic nitrogen fixation with nitrogen-fixing bacteria in the root nodules is a distinctly beneficial metabolic process in legume plants. Legumes control the nodule number and nodulation zone through a systemic negative regulatory system between shoot and root. Mutation in the soybean NTS gene encoding GmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule development called hypernodulation. To examine the effect of nts mutation on the gene expression profile in the leaves, suppression subtractive hybridization was performed with the trifoliate leaves of nts mutant 'SS2-2' and the wild-type (WT) parent 'Sinpaldalkong2', and 75 EST clones that were highly expressed in the leaves of the SS2-2 mutant were identified. Interestingly, the expression of jasmonate (JA)-responsive genes such as vspA, vspB, and Lox2 were upregulated, whereas that of a salicylate-responsive gene PR1a was suppressed in the SS2-2 mutant. In addition, the level of JA was about two-fold higher in the leaves of the SS2-2 mutant than in those of the WT under natural growth conditions. Moreover, the JA-responsive gene expression persists in the leaves of SS2-2 mutant without rhizobia infection in the roots. Taken together, our results suggest that the nts mutation increases JA synthesis in mature leaves and consequently leads to constitutive expression of JA-responsive genes which is irrelevant to hypernodulation in the root.
Keywords
Hypernodulation; Jasmonate; NTS; Salicylate; Soybean; SSH; vspA; vspB;
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