• Title/Summary/Keyword: volumetric productivity

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Ethanol Production from Xylose by Pichia stipitis Using Cell-recycled Bilreactor (Pichia stipitis 세포의 재순환 생물반응기를 이용한 Xylose로부터 Ethanol 생산)

  • 박영민;정인식;크리스론식;이윤형
    • KSBB Journal
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    • v.4 no.2
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    • pp.74-77
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    • 1989
  • To increase the volumetric productivity a contimuous cell-recycled system was implemented. Cell concentrations between 9.2 and 15.0 g/1 were obtatined in the continuous fermentor study. At a 4% xylose feed and a specific oxygen supply rate(SOSR) of 1.04 g O2.hr-g DCW the ethanol yield was 0.36% at dilution rate. This represented a 26-% increase over that of th batch fermentation.

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Fortification of Amino Acids to Improve Hybridoma Cell Growth and Monoclonal Antibody Production in Perfusion Culture (Perfusion배양시 세포성장 및 항체생산 향상을 위한 아미노산의 보강)

  • 이수영;최병욱;오한규;윤정원;전복환;변태호;박송용
    • KSBB Journal
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    • v.14 no.2
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    • pp.188-191
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    • 1999
  • We have investigated the fortifying effect of amino acids on the cell growth and productivity during the perfusion culture of hybridoma vR8 cells in serum-free media. Through the quantitative analysis of amino acids and metabolites in perfusion culture, we found that many amino acids(glutamine, histidine, arginine, methionine, isoleucine, leucine, phenylalanine, tryptophane) were heavily consumed at cell density of $1.06{\times}10^7$cells/mL. Due to amino acid depletion, cells died suddenly. So we supplemented the media with those amino acids by 30-170%. As a result, were could increase maximum cell density by 270%, average specific productivity by 175%, and average volumetric productivity by 560% in this fortified media, GC-HY-S2.

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Enhancement of a parabolic face working accuracy using volumetric error compensation of NC milling machine (NC 밀링머신의 Volumetric 오차보상을 통한 포물면 가공의 정밀도 향상)

  • 이찬호;정을섭;이응석;김성청
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2000.05a
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    • pp.917-921
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    • 2000
  • One of the major limitations of productivity and quality in machining is machining accuracy of the machine tools. The machining accuracy is affected by geometric, volumetric errors of the machine tools. This paper suggests the enhancement method of machining accuracy for precision machining of high quality metal reflection mirror or optics lens, etc. In this paper, we study 1) the compensation of linear pitch error with NC controller compensation function using laser interferometer measurement, 2) the method for enhancing the accuracy of NC milling machining by modeling and compensation of volumetric error, 3) the generation of the parabolic face profile. And the method is verified by the parabolic face machining experiment with a vertical three axes NC milling machine. After this study, we will inspect using On-machine measurement and study the repetitive machining by a compensated path

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Continuous Propionic Acid Production from Cheese Whey Using In Situ Spin Filter

  • Gupta, Achin;Srivastava, Ashok K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.1
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    • pp.1-5
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    • 2001
  • The potential use of spin filter device to retain Propionibacterium acidipropionici in the bioreactor under continuous mode of fermentation, and improve acid productivity, was examined. The yield of propionic acid based on lactose concentration was 51% in batch and 54% in continuous (dilution rate = 0.05 h(sup)-1) operation. The yield in continuous fermentation with cell retention using spin filter of 10 micron size (dilution rate = 0.05 h(sup)-1) was even higher at 70% (w/w). The volumetric productivity under batch and continuous mode of operation were 0.312g L(sup)-1 h(sup)-1 and 0.718g L(sup)-1 h(sup)-1 respectively. Continuous fermentation with cell retention demonstrated even higher volumetric productivities at 0.98g L(sup)-1 h(sup)-1 with out clogging problems. It could be used for utilization of cheese whey to produce propionic acid at higher yield and productivities.

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Bioreactor Cultures of Lithospermum erythrorhizon for Shikonin Production with In Situ Extraction (동시 추출을 겸한 생물반응기에서 Lithospermum erythrorhizon 배양에 의한 shikonin 생산)

  • 김동진;장호남
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.525-529
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    • 1990
  • Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400$\mu g$ shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857$\mu g$ shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.

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Repeated-batch Culture of Immobilized Gibberella fujikuroi B9 for Gibberellic Acid Production: An Optimization Study

  • Kim, Chang-Joon;Lee, Sang-Jong;Chang, Yong-Keun;Chun, Gie-Taek;Jeong, Yeon-Ho;Kim, Sung-Bae
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.6
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    • pp.544-549
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    • 2006
  • The performance of immobilized fungal cells on celite beads for the production of gibberrelic acid was investigated in flasks and 7-L stirred-tank reactor. Repeated incubations of immobilized fungal cells increased cell concentrations and volumetric productivity. The maximum volumetric productivity obtained in the immobilized-cell culture was 3-fold greater than that in suspended-cell culture. The concentration of cotton seed flour (CSF), among the various nutrients supplied, most significantly influenced productivity and operational stability. Notably, insoluble components in CSF were found to be essential for production. CSF at 6 g/L with 60 g/L glucose was found to be optimal for gibberellic acid production and stable operation by preventing excessive cell growth.

The Effect of Media Feeding Rate on the Production of Monoclonal Antibody Production in the Fed-batch Culture of Hybridoma (하이브리도마 세포의 유가식 배양에서 배지첨가속도가 단일클론 항체 생산에 미치는 영향)

  • 곽원재;최태부;박정극
    • Microbiology and Biotechnology Letters
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    • v.19 no.3
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    • pp.272-280
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    • 1991
  • The effect of media feeding rate on cell growth and monoclonal antibody production in the fed-batch culture ot hybridoma A4W was studied. In the batch culture, the highest specific antibody production rate was observed at the begining of the culture period but its value tended to decrease rapidly with the culture time. The final antibody concentration and volumetric productivity was 65 $\mu g$/ml and 13 mg Mab/l/day, respectively. In the fed-batch culture, the specific antibody production rate, $q_p$ rebounded sharply within a few hours after the media feeding was started and it remained high until the end of culture if the media feeding was continued. The final antibody concentration was 220 $\mu g$/ml and the volumetric productivity was 45.1 mg/l/day. Further increase in final antibody concentration was achieved by applying a modified media of which component was fortified with glucose and glutamine, hence the final antibody concentration in this case was 270 $\mu g$/ml and the volumetric productivity was 51.8 mg/lday, which is as four tinlcs as high cuixparinf! to that of batch culture.

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Economic Alternative for Volumetric Module Lifting/Offloading (볼류메트릭 모듈 양중 및 인양 대안에 관한 연구)

  • Song, Seung-Ho;Kwon, Woo-Bin;Choi, Jin-Ouk;Cho, Hun-Hee
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2023.05a
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    • pp.75-76
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    • 2023
  • The construction industry's lack of experience and expertise makes it difficult for projects to realize the full benefits of implementing modular construction. Such project performance-hindering elements are often labeled as modular challenges. The added requirement for the transportation of the finished volumetric module is one aspect of the 'module transportation logistics,' the under-researched modular challenge that can prevent projects from incurring maximum cost and productivity benefits. The typical module transportation phases include lifting, transporting, and offloading. From conducting a literature review, this paper aims to investigate the equipment commonly adopted to lift and offload the module and validate its economic efficiency by comparing it with the alternative lifting/offloading equipment used in the two case projects. The results showed that hydraulic jacks are an economic alternative to the crane for lifting/offloading the module. The increase in single-module projects with smaller budgets made crane usage economically undesirable, and this study suggested a viable option for a more economical alternative.

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Enhanced Production of Valuable Bioactive Metabolites in Submerged Cultures of Medicinal Mushroom Ganoderma lucidum by Manipulation of Oxygen Supply

  • Zhong, Jian-Jiang;Fang, Qing-Hua;Tang, Ya-Jie
    • Journal of Plant Biotechnology
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    • v.4 no.3
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    • pp.109-115
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    • 2002
  • Submerged cultures of Ganoderma lucidum, a valuable mushroom in traditional Chinese medicine, were used for production of bioactive Banoderic acids and Ganoderma polysaccharides. The significant effects of oxygen supply were demonstrated in both shake flasks and bioreactors. By changing the medium loading volume in a shake flask, a different value of initial volumetric oxygen transfer coefficient ($K_L$a) was obtained, and a higher $K_L$a value led to a higher biomass density and a higher productivity of both intracellular polysaccharide and ganoderic acid. In a stirred bioreactor, at an initial $K_L$a of 78.2 $h^{-1}$, a maximal cell concentration of 15.6 g/L by dry weight was obtained, as well as a maximal intracellular polysarcharide (IPS) production of 2.2 g/L and its maximal productivity of 220 mg/(L.d). An increase of initial $K_L$a led to a higher production and productivity of GA, and the GA production and productivity at an initial $K_L$a of 96.0 $h^{-1}$ was 1.8-fold those at an initial $K_L$a of 16.4 $h^{-1}$. The fundamental information obtained in this study may be useful for efficient large-scale production of these valuable bioactive products by the submerged cultures.

Identification of Green Alga Chlorella vulgaris Isolated from Freshwater and Improvement Biodiesel Productivity via UV Irradiation

  • Gomaa, Mohamed A.;Refaat, Mohamed H.;Salim, Tamer M.;El-Sayed, Abo El-Khair B.;Bekhit, Makhlouf M.
    • Microbiology and Biotechnology Letters
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    • v.47 no.3
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    • pp.381-389
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    • 2019
  • Chlorella vulgaris was isolated from the Nile River, Qalubia Governorate, Egypt, for possible use in biodiesel production. BG-II nutrient growth media was used for isolation and laboratory growth. Identification was performed via 18S rRNA gene amplification, followed by sequencing. The alga was exposed to UV-C (254 nm) for 15, 30, and 45 s to improve dry weight accumulation and to increase the oil production. Daily measurements of dry weight ($g{\cdot}l^{-1}$) were performed; oil content and volumetric lipid productivity were also determined. UV-C exposure led to an increase in the volumetric lipid productivity by 27, 27.3, and $32.4mg{\cdot}l^{-1}{\cdot}d^{-1}$ with 15, 30, and 45 s, respectively, as compared with the control, which resulted in $18mg{\cdot}l^{-1}{\cdot}d^{-1}$. Of the examined mutants, the one with the highest productivity was re-irradiated by UV-C (254 nm) for 15, 30, 45, and 60 s. For 15 s of exposure time, the oil content increased to 34%, while it was 31% at 30 s; further, it decreased to 22% at 45 and 60 s exposures. The fatty acid methyl ester profile was 82.22% in the first mutant at 45 s, compared with the wild strain that contained a total of 66.01% of FAs. Furthermore, the highest levels of polyunsaturated fatty acid methyl ester were observed in the mutant exposed for 45 s, and it reached 11.41%, which reduced the cetane number to 71.3.