Enhanced Production of Valuable Bioactive Metabolites in Submerged Cultures of Medicinal Mushroom Ganoderma lucidum by Manipulation of Oxygen Supply

  • Zhong, Jian-Jiang (State Key Laboratory of Bioreactor Engineering(China), East China University of Science and Technology, China) ;
  • Fang, Qing-Hua (State Key Laboratory of Bioreactor Engineering(China), East China University of Science and Technology, China) ;
  • Tang, Ya-Jie (State Key Laboratory of Bioreactor Engineering(China), East China University of Science and Technology, China)
  • Published : 2002.09.01


Submerged cultures of Ganoderma lucidum, a valuable mushroom in traditional Chinese medicine, were used for production of bioactive Banoderic acids and Ganoderma polysaccharides. The significant effects of oxygen supply were demonstrated in both shake flasks and bioreactors. By changing the medium loading volume in a shake flask, a different value of initial volumetric oxygen transfer coefficient ($K_L$a) was obtained, and a higher $K_L$a value led to a higher biomass density and a higher productivity of both intracellular polysaccharide and ganoderic acid. In a stirred bioreactor, at an initial $K_L$a of 78.2 $h^{-1}$, a maximal cell concentration of 15.6 g/L by dry weight was obtained, as well as a maximal intracellular polysarcharide (IPS) production of 2.2 g/L and its maximal productivity of 220 mg/(L.d). An increase of initial $K_L$a led to a higher production and productivity of GA, and the GA production and productivity at an initial $K_L$a of 96.0 $h^{-1}$ was 1.8-fold those at an initial $K_L$a of 16.4 $h^{-1}$. The fundamental information obtained in this study may be useful for efficient large-scale production of these valuable bioactive products by the submerged cultures.



  1. Anal Chem v.28 Colorimetric method for determination of sugars and related substances Dubois M;Gilles KA;Hamilton JK;Rebers PA;Smith F https://doi.org/10.1021/ac60111a017
  2. Phytochemistry v.49 Anti-HIV-1 and anti-HIV-protease substances from Ganoderma lucidum El-Mekkaway S;R. Meselhy M;Nakamura N;Tezuka Y;Hattori M;Kakiuchi N;Shimotohno K;Kawahata T;Otake T https://doi.org/10.1016/S0031-9422(98)00254-4
  3. Biotechnol Prog v.18 Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderma lucidum Fang QH;Zhong JJ https://doi.org/10.1021/bp010136g
  4. Process Biochem v.37 Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum Fang QH;Zhong JJ https://doi.org/10.1016/S0032-9592(01)00278-3
  5. Biochem Eng J v.10 Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites - ganoderic acid and polysaccharide Fang QH;Zhong JJ https://doi.org/10.1016/S1369-703X(01)00158-9
  6. Appl Biochem Biotechnol v.67 Study on the production of a xylanolytic complex from Penicillium canescens 10-10c Gaspar A;Cosson T;Roques C;Thonart P https://doi.org/10.1007/BF02787840
  7. Biotechnol Bioeng v.64 Improvement of red pigment/citrinin production ratio as a function of environmental conditions by Monascus ruber Hajjaj H;Blane PJ;Groussac E;Goma G;Uribelarrea JL;Loubiere P https://doi.org/10.1002/(SICI)1097-0290(19990820)64:4<497::AID-BIT12>3.0.CO;2-Q
  8. Planta Med v.4 Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies Hikino H;Konno C;Mirin Y;Hayashi T
  9. Mikrobiologiya v.50 Influence of the acidity of the medium, conditions of aeration and temperature on pullulan biosynthesis polyploid strains of Pullaria (Aureobasidium) pullulans Ishmentskii AA;Kondrat'eva TF;Smut'ko AN
  10. J Biosci Bioeng v.88 Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor Lee KM;Lee SY;Lee HY https://doi.org/10.1016/S1389-1723(00)87094-2
  11. Biotechnol Bioeng v.51 Noninvasive oxygen measurements and mass transfer considerations in tissue culture flasks Randers-Eichhorn L;Bartlett RA;Frey DD;Rao G https://doi.org/10.1002/(SICI)1097-0290(19960820)51:4<466::AID-BIT10>3.0.CO;2-G
  12. Ind Microbiol v.9 Enhanced glucan formation of filamentous fungi by effective mixing, oxygen limitation and fed-batch processing Rau U;Gura E;Olszewski E;Wangner F
  13. ACS Symp Ser 547 Food Phytochemicals for Cancer Prevention Ⅱ Natural products and biological activities of the Chinese medicinal fungus Ganoderma lucidum Shiao MS;Lee KR;Lee JL;Cheng TW
  14. Agric Biol Chem v.49 Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing fermentation of mycelium of Ganoderma lucidum Sone Y;Okuda R;Wada N;Kishida E;Misaki A https://doi.org/10.1271/bbb1961.49.2641
  15. Advances in Applied Biotechnology Submerged fermentation of higher fungi for production of valuable metabolites (a review) Tang YJ;Zhong JJ;Zhong JJ(ed.)
  16. Enzyme Microb Technol v.31 Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid Tang YJ;Zhong JJ https://doi.org/10.1016/S0141-0229(02)00066-2
  17. Jpn Kokai Tokkyo Koho JP 04304890 Manufacture of ganoderic acid by fermentation of Ganoderma lucidum Tsujikura Y;Higuchi T;Miyamoto Y;Sato S
  18. Biotechnol Bioeng v.51 A novel centrifugal impeller bioreactor. Ⅱ. Oxygen transfer and power consumption Wang SJ;Zhong JJ https://doi.org/10.1002/(SICI)1097-0290(19960905)51:5<520::AID-BIT3>3.0.CO;2-E
  19. J Nat Prod v.64 Cytotoxicity of Ganoderma lucidum triterpenes Wu TS;Shi LS;Kuo SC https://doi.org/10.1021/np010115w
  20. Process Biochem v.33 The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged fermentation Yang FC;Liau CB https://doi.org/10.1016/S0032-9592(98)00023-5
  21. J Ferment Technol v.43 Studies of submerged culture of basidiomycetes (Ⅱ) the effect of exygen on the respiration of Shiitake (Lentinus edodes) Yoshida T;Shimizu T;Taguchi H;Teramoto S
  22. J Ferment Technol v.45 Studies on submerged cultures of basidiomycetes (Ⅲ): The oxygen transfer within the pellets of Lentinus edodes Yoshida T;Shimizu T;Taguchi H;Teramoto H