• Asian-Australasian Journal of Animal Sciences
• /
• 제19권4호
• /
• pp.500-506
• /
• 2006

An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum ${\alpha}$-tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL-${\alpha}$-tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum ${\alpha}$-tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum ${\alpha}$-tocopherol concentration (${\mu}g/100ml$) was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the ${\alpha}$-tocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.

• Fisheries and Aquatic Sciences
• /
• 제26권9호
• /
• pp.525-534
• /
• 2023

Vitamin C plays an important role for fish survival, growth and disease resistance. However, the optimal vitamin C for rearing red drum Sciaenops ocellatus juveniles in Vietnam is not known. To address this issue, a 70-day feeding trial was conducted to evaluate the optimal dietary vitamin C requirements for red drum juveniles. Seven isonitrogenous (55.35% protein) and isolipidic (9.07% lipid) diets were formulated to include graded vitamin C concentrations of 23.2, 124.5, 235.2, 423.8, 626.7, 824.6, and 1,027.3 mg/kg, respectively. The results showed that fish fed on 423.8 mg/kg vitamin C diet had the highest growth rate, which can be linked to the increased feed utilization. Broken-line analysis indicated that the optimal dietary vitamin C requirements of red drum juveniles were 342.92 and 405.80 mg/kg for growth parameters, feed utilization, body composition and biochemical parameters of serum. Based on these parameters the optimal vitamin C supplementation level for red drum juveniles was estimated in the range of 342.92-405.80 mg/kg vitamin C in the diets with direct applications in producing artificial feed for rearing juveniles of this species in Vietnam.

• Nutrition Research and Practice
• /
• 제15권3호
• /
• pp.309-318
• /
• 2021

BACKGROUD/OBJECTIVES: Data regarding the effects of poly-γ-glutamic acid (γ-PGA) on sleep status are limited. This study aimed to test whether γ-PGA and vitamin B₆ (VitB6) supplements improve sleep duration and quality. SUBJECTS/METHODS: A factorial randomized, double-blinded, placebo-controlled crossover study included 47 adults (25 men and 22 women) who were free of chronic disease. Stratified randomized allocation considered age and gender for three interventions, group A (supplementation with γ-PGA 600 mg; n = 16), group B (supplementation with VitB6 100 mg; n = 14), and group C (dual supplementation of both γ-PGA 600 mg and VitB6 100 mg; n = 17). Participants underwent a 1-mon intervention period, followed by a 1-mon washout period, and then a second 1-mon intervention period. Differences (mean ± SD) in nighttime sleep status before and after supplementation were compared between the placebo and intervention groups using nonparametric tests. RESULTS: Significant changes in sleep duration (0.27 ± 0.98 h, P < 0.05) and the Pittsburgh Sleep Quality Index global score (-0.52 ± 1.58, P < 0.05) indicating improved sleep status were observed in the intervention compared with the placebo of group C while no significant changes were observed in groups A and B. No statistical significance was detected between the intervention and the placebo; however, there was a greater increase in the group C intervention (4.59 ± 38.5 ng/mL) in serum serotonin concentrations than the groups A and B interventions. No side effects were observed. CONCLUSIONS: On the basis of these findings, the dual supplementation of γ-PGA and VitB6 may be effective as functional food components to improve nighttime sleep status.

• 한국가금학회지
• /
• 제40권1호
• /
• pp.31-40
• /
• 2013

본 연구는 브로일러에서 비타민 C와 E의 첨가 급여가 성장 능력 및 개체별 스트레스 경감 정도에 미치는 영향을 살펴보고자 하였다. 스트레스 반응 정도는 혈액과 각 조직별 세포들에 대한 텔로미어 함량, DNA 손상율 및 열손상단백질 유전자(HSP, HMGCR) 발현율을 분석하고 고찰하였다. 텔로미어 함량 및 감축율은 양적 형광접합보인법(Q-FISH)으로 분석하였고, DNA 손상율은 comet assay로 분석하였다. 열손상단백질 유전자 발현율은 HSP70, HSP90-${\alpha}$, HSP90-${\beta}$ 및 HMGCR을 표적으로 하여 real-time PCR로 분석하였다. 시험 결과, 급여 처리구 간에 체중, 증체량, 사료 섭취량, 사료 요구율 및 생존율 등 생산 능력의 차이는 없는 것으로 나타났다. 텔로미어 감축율에 있어서는 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 감축율을 보여 스트레스 경감의 효과를 나타내었다. DNA 손상율 또한 모든 비타민 첨가 급여구가 대조구에 비해 유의하게 낮은 양상을 보였다. HMGCR, HSP90-${\alpha}$ 및 HSP90-${\beta}$의 유전자 발현율에 있어서도 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 발현율을 나타내어 스트레스 경감 효과를 나타내었다. 이상의 결과에 따라 브로일러에 사료 내 비타민 E의 첨가 급여(100 mg/kg feed)는 성장 능력의 저하 없이 개체의 생리적 스트레스 정도를 경감시키는 바람직한 항산화 제재로 사료된다.

• 한국어병학회지
• /
• 제6권2호
• /
• pp.177-189
• /
• 1993

1. 과산화물가가 90.4mEq/kg인 산화사료에 Vitamin E와 C의 함량을 각각 달리 하여 80일간 Ceroid 예방실험을 행하였다. 2. Ceroid증을 예방하기 위하여 산화사료에 Vitamin C와 E를 첨가한 모든 실험군 및 무첨가한 대조군에서의 성장 비교에서 대조군은 산화사료에 의한 성장 장애가 나타났으나 실험군에서는 정상성장을 보였다. 3. 병리조직학적 관찰에서 대조군은 실험과정 일수에 따라 조직변화가 심하였으나 Vitamin E와 C를 첨가한 실험군별 조직상태는 Vitamin E 1mg/g diet와 Vitamin C 2IU/g diet를 첨가한 실험군에서의 예방효과가 가장 양호하게 나타났다.

• Nutrition Research and Practice
• /
• 제8권5호
• /
• pp.533-538
• /
• 2014

BACKGROUND/OBJECTIVES: We investigated the effect of Pycnogenol (Pyc) on survival and immune dysfunction of C57BL/6 mice induced by low micronutrient supplementation. MATERIALS/METHODS: Female C57/BL/6 mice were fed a diet containing 7.5% of the recommended amount of micronutrients for a period of 12 wks (immunological assay) and 18 wks (survival test). For immunological assay, lymphocyte proliferation, cytokine regulation, and hepatic oxidative status were determined. RESLUTS: Pyc supplementation with 50 and $100mg{\cdot}kg^{-1}{\cdot}bw{\cdot}d^{-1}$ resulted in partial extension of the median survival time. Pyc supplementation led to increased T and B cell response against mitogens and recovery of an abnormal shift of cytokine pattern designated by the decreased secretion of Th1 cytokine and increased secretion of Th2 cytokine. Hepatic vitamin E level was significantly decreased by micronutrient deficiency, in accordance with increased hepatic lipid peroxidation level. However, Pyc supplementation resulted in a dose-dependent reduction of hepatic lipid peroxidation, which may result from restoration of hepatic vitamin E level. CONCLUSION: Findings of this study suggest that Pyc supplementation ameliorates premature death by restoring immune dysfunction, such as increasing lymphocyte proliferation and regulation of cytokine release from helper T cells, which may result from the antioxidative ability of Pyc.

• 한국가금학회:학술대회논문집
• /
• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
• /
• pp.69-79
• /
• 2004

본 연구에서는 사료 중 비타민 C(200 mg/kg)와 비타민 E(250 mg/kg)의 첨가가 heat stress하 육용종계의 난각 품질에 미치는 영향을 조사하였다. 83주령의 강제환우된 Ross 육용종계 160수를 공시하여 4처리 4반복 그리고 반복당 10수를 무작위로 개별 케이지에 수용하였다. 4처리구 중 대조구는 비타민 C와 E를 추가로 첨가하지 않았으며 시험구는 비타민 C 처리구, 비타민 E 처리구, 그리고 비타민 C/E 동시 처리구로 하였다. 실온 25 $^{\circ}C$에서 10일간의 적응기간 후에 32 $^{\circ}C$에서 3주간 실험하였다. 실험기간 중 산란율은 급속히 떨어졌으나 처리구간에 유의한 차이가 없었으나 난중, 비중, 난각두께, SWUSA, pucture force와 난각 파괴강도에서는 비타민 C/E 처리구가 대조구에 비해 유의하게 높았다(P<0.05). 비타민 C 처리구는 경골 파괴강도에서 37.16 kg으로 대조구 및 비타민 E 처리구에 비해 통계적으로 유의하게 높았다(P<0.05).한편, 혈중 corticosterone 수준(5.97 ng/ml)은 대조구가 다른 시험구들보다 유의하게 높았다(P<0.05). Heat stress는 백혈구 중 heterophil의 비율을 높이고 lymphocyte의 비율은 낮추어 H/L ratio를 변화시켰다. 시험구의 H/L ratio는 대조구보다 낮았으나 통계적으로 유의한 차이를 나타내지 않았다. 결론적으로 사료 중 비타민 C(200 mg/kg)와 비타민 E(250 mg/kg)의 첨가는 육용종계에서 heat stress에 의한 영향을 경감하여 난각품질 및 경골강도의 약화를 방지한다고 할 수 있겠다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권2호
• /
• pp.225-231
• /
• 2008

Oxidative stress plays a crucial role in the laying stage which is a critical period for chick survival. We investigated the relationship of neonatal chick performance, brain antioxidant status and vitamin E supplementation level in hens. Starting at 17 weeks, hens were randomly divided into five groups. The control group received a basal diet without supplemental vitamin E (VE, dl-${\alpha}$-tocopherol acetate). Other groups received the same basal diet supplemented with vitamin E (40, 80, 120 and 160 mg/kg) through growth to egg production. Hens were artificially inseminated at 28 weeks of age and egg yolks were collected at day two. All remaining eggs were hatched. Yolk vitamin E content, hatchability and fertility of eggs were evaluated. Brains of the newly hatched chicks were further evaluated for their oxidative stress status, antioxidative status and vitamin E levels. Increased reproductive performance was observed in fertility and hatchability in the group supplemented at 40 mg/kg. Egg yolk and neonatal brain ${\alpha}$-tocopherol was highest in eggs from hens fed 120 mg/kg and 80 mg/kg supplemental vitamin E, respectively. Brain MDA, ROS and iron levels were significantly higher in unsupplemented hens (p<0.01). SOD activity was significantly higher in the group supplemented at 160 mg/kg than in all other groups. We concluded that maternal supplementation of vitamin E had beneficial effects on fertility, hatchability of eggs, neonatal brain oxidative status and SOD activity.

• Journal of Nutrition and Health
• /
• 제42권2호
• /
• pp.107-118
• /
• 2009

In patients with type 2 diabetes, oxidative stress could be increased by their metabolic changes. Elevated plasma homocysteine is considered as one of markers of enhanced oxidative stress. Due to oxidative stress, some complications like cardiovascular or renal diseases may develop in type 2 diabetes patients. Plasma homocysteine concentration may be increased if folate status were inadequate. Protective effects against oxidative stress may be diminished if the status of anti-oxidative nutrient as vitamin C was poor. It is, therefore, important to maintain adequate status of folate and vitamin C in type 2 diabetes patients. Thus, this study was performed to determine the effects of supplementation of folate and/or ascorbate on blood glycated hemoglobin ($HbA_{1c}$) level, serum concentrations of homocysteine and cholesterol, plasma oxidized low density-lipoprotein (LDL), concentration and plasma glutathione peroxidase (GSH-Px) activity in the patients with type 2 diabetes. A total of 92 type 2 diabetes patients participated voluntarily with written consents. They were divided into one of the four experimental groups; Control (C), Folate-supplemented (F), Ascorbate-supplemented (A), and Folate plus ascorbate-supplemented (FA). The subjects in C were taken placebo, those in F were supplemented 1 mg of folate, those in A received 1,000 mg of ascorbate, and those in FA were given 1 mg of folate plus 1,000 mg of ascorbate daily for 4 weeks. Supplementation of folate or ascorbate resulted to increase serum folate level or plasma ascorbate concentration apparently, respectively. Folate supplementation not ascorbate seemed to decrease plasma concentrations of homocysteine and oxidized LDL and reduce plasma GSH-Px activity. There might not be synergic effect of the supplementation of folate plus ascorbate. The results indicate that oxidative stress in the patients with type 2 diabetes may lower mainly by folate supplementation.

• 생명과학회지
• /
• 제20권4호
• /
• pp.511-518
• /
• 2010

전 세계적으로 알레르기 환자가 증가하고 있고, 특히 운동이 알레르기 질환을 증가 시켜 운동 유발성 알레르기에 대한 연구가 주목되고 있다. 운동 유발성 알레르기 질환은 운동 중과 운동 후 혈관부종, 두드레기, 호흡, 위와 장계의 증후 및 아나플락시스 등이 나타나는 질환이다. 따라서 본 연구는 운동 유발성 알레르기 질환의 분석 및 비타민 C와 catalase 투여에 대한 항 알레르기 효과와 활성 산소종 생성 조절제 들의 상호작용을 규명하고 전반적인 운동 유발성 알레르기 질환의 치료효과를 규명하는데 그 목적이 있다. 연구방법으로는 무병원성환경에서 사육한 생후 7주령의 BALE/c마우스(Female)를 그룹핑 하여 통제군과 훈련군에게 각각 알레르기를 유발한 후, 서로 다른 기간의 운동 효과를 분석하였고, 운동 유발성 알레르기 군에게 비타민c와 catalase를 투여하여 투여 효과를 분석하였다. 본 연구결과 운동 유발성 알레르기 질환은 훈련기간이 늘어남에 따라 증가함을 알 수 있었고, 이러한 운동 유발성 알레르기 질환과 쇼크사는 복강 림프구에서 발현하는 ROS는 큰 연관이 있다는 것, 그리고 운동 유발성 알레르기 치료 효과는 catalase를 투여하는 것 보다 비타민을 투여하는 것이 더욱 효과적임을 알 수 있었다. 추후 이에 대한 원인을 규명하는 기전적 연구가 필요할 것으로 여겨진다.