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http://dx.doi.org/10.4162/nrp.2021.15.3.309

Effects of poly-gamma-glutamic acid and vitamin B6 supplements on sleep status: a randomized intervention study  

Garcia-Garcia, Carolina (Department of Foods and Nutrition, College of Science and Technology, Kookmin University)
Baik, Inkyung (Department of Foods and Nutrition, College of Science and Technology, Kookmin University)
Publication Information
Nutrition Research and Practice / v.15, no.3, 2021 , pp. 309-318 More about this Journal
Abstract
BACKGROUD/OBJECTIVES: Data regarding the effects of poly-γ-glutamic acid (γ-PGA) on sleep status are limited. This study aimed to test whether γ-PGA and vitamin B6 (VitB6) supplements improve sleep duration and quality. SUBJECTS/METHODS: A factorial randomized, double-blinded, placebo-controlled crossover study included 47 adults (25 men and 22 women) who were free of chronic disease. Stratified randomized allocation considered age and gender for three interventions, group A (supplementation with γ-PGA 600 mg; n = 16), group B (supplementation with VitB6 100 mg; n = 14), and group C (dual supplementation of both γ-PGA 600 mg and VitB6 100 mg; n = 17). Participants underwent a 1-mon intervention period, followed by a 1-mon washout period, and then a second 1-mon intervention period. Differences (mean ± SD) in nighttime sleep status before and after supplementation were compared between the placebo and intervention groups using nonparametric tests. RESULTS: Significant changes in sleep duration (0.27 ± 0.98 h, P < 0.05) and the Pittsburgh Sleep Quality Index global score (-0.52 ± 1.58, P < 0.05) indicating improved sleep status were observed in the intervention compared with the placebo of group C while no significant changes were observed in groups A and B. No statistical significance was detected between the intervention and the placebo; however, there was a greater increase in the group C intervention (4.59 ± 38.5 ng/mL) in serum serotonin concentrations than the groups A and B interventions. No side effects were observed. CONCLUSIONS: On the basis of these findings, the dual supplementation of γ-PGA and VitB6 may be effective as functional food components to improve nighttime sleep status.
Keywords
Functional food; vitamin B6; sleep;
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1 Park SE, Kim HM, Kim DH, Kim J, Cha BS, Kim DJ. The association between sleep duration and general and abdominal obesity in Koreans: data from the Korean National Health and Nutrition Examination Survey, 2001 and 2005. Obesity (Silver Spring) 2009;17:767-71.   DOI
2 Abrams RM. Sleep deprivation. Obstet Gynecol Clin North Am 2015;42:493-506.   DOI
3 Hwang SH, Kang JM, Seo JH, Han KD, Joo YH. Association between sleep duration and dizziness in Korean women: the Korea National Health and Nutrition Examination Survey. J Korean Med Sci 2019;34:e226.   DOI
4 Cunnington D, Junge MF, Fernando AT. Insomnia: prevalence, consequences and effective treatment. Med J Aust 2013;199:S36-40.
5 Morin CM, Vallieres A, Guay B, Ivers H, Savard J, Merette C, Baillargeon L. Cognitive behavior therapy, singly and combined with medication, for persistent insomnia: acute and maintenance therapeutic effects. JAMA 2011;301:2005-15.   DOI
6 Ebben M, Lequerica A, Spielman A. Effects of pyridoxine on dreaming: a preliminary study. Percept Mot Skills 2002;94:135-40.   DOI
7 Sohn SI, Kim DH, Lee MY, Cho YW. The reliability and validity of the Korean version of the Pittsburgh Sleep Quality Index. Sleep Breath 2012;16:803-12.   DOI
8 Scholey A, Benson S, Gibbs A, Perry N, Sarris J, Murray G. Exploring the effect of lactium™ and Zizyphus complex on sleep quality: a double-blind, randomized placebo-controlled trial. Nutrients 2017;9:154.   DOI
9 Buysse DJ, Reynolds CF 3rd, Monk TH, Berman SR, Kupfer DJ. The Pittsburgh Sleep Quality Index: a new instrument for psychiatric practice and research. Psychiatry Res 1989;28:193-213.   DOI
10 Spira AP, Beaudreau SA, Stone KL, Kezirian EJ, Lui LY, Redline S, Ancoli-Israel S, Ensrud K, Stewart A; Osteoporotic Fractures in Men Study. Reliability and validity of the Pittsburgh Sleep Quality Index and the Epworth Sleepiness Scale in older men. J Gerontol A Biol Sci Med Sci 2012;67:433-9.
11 Korean Nutrition Society; Ministry of Health and Welfare. Dietary Reference Intakes for Koreans. Seoul: Korean Nutrition Society; 2015.
12 Johns MW. A new method for measuring daytime sleepiness: the Epworth Sleepiness Scale. Sleep 1991;14:540-5.   DOI
13 Ministry of Food and Drug Safety. Current Status of Functional Ingredients for Health Functional Food in Korea. Cheongju: Ministry of Food and Drug Safety; 2016.
14 Lee H, Chang MJ, Kim SH. Effects of poly-γ-glutamic acid on serum and brain concentrations of glutamate and GABA in diet-induced obese rats. Nutr Res Pract 2010;4:23-9.   DOI
15 Beaudreau SA, Spira AP, Stewart A, Kezirian EJ, Lui LY, Ensrud K, Redline S, Ancoli-Israel S, Stone KL; Study of Osteoporotic Fractures. Validation of the Pittsburgh Sleep Quality Index and the Epworth Sleepiness Scale in older black and white women. Sleep Med 2012;13:36-42.   DOI
16 Daneman R, Prat A. The blood-brain barrier. Cold Spring Harb Perspect Biol 2015;7:a020412.   DOI
17 Kim S, Jo K, Hong KB, Han SH, Suh HJ. GABA and l-theanine mixture decreases sleep latency and improves NREM sleep. Pharm Biol 2019;57:65-73.   DOI
18 Park JH, Choi JC, Sung MH, Kang JH, Chang MJ. High molecular weight poly-gamma-glutamic acid regulates lipid metabolism in rats fed a high-fat diet and humans. J Microbiol Biotechnol 2011;21:766-75.   DOI
19 King PW. Climbing Maslow's Pyramid. In: Introducing Abraham Maslow and His Pyramid. Kibworth Harcourt: Troubador Publishing Ltd.; 2009. p.3-21
20 Park S, Cho MJ, Chang SM, Bae JN, Jeon HJ, Cho SJ, Kim BS, Chung IW, Ahn JH, Lee HW, Hong JP. Relationships of sleep duration with sociodemographic and health-related factors, psychiatric disorders and sleep disturbances in a community sample of Korean adults. J Sleep Res 2010;19:567-77.   DOI
21 Kim TW, Lee TY, Bae HC, Hahm JH, Kim YH, Park C, Kang TH, Kim CJ, Sung MH, Poo H. Oral administration of high molecular mass poly-gamma-glutamate induces NK cell-mediated antitumor immunity. J Immunol 2007;179:775-80.   DOI
22 Oyunbileg E, Jun N, Yoon D, Baik I. Effects of poly-gamma-glutamic acid on inflammatory and metabolic biomarkers in sleep-restricted rats. Sleep Biol Rhythms 2018;16:399-404.   DOI
23 Luo Z, Guo Y, Liu J, Qiu H, Zhao M, Zou W, Li S. Microbial synthesis of poly-γ-glutamic acid: current progress, challenges, and future perspectives. Biotechnol Biofuels 2016;9:134.   DOI
24 Ito Y, Tanaka T, Ohmachi T, Asada Y. Glutamic acid independent production of poly (γ-glutamic acid) by Bacillus subtilis TAM-4. Biosci Biotechnol Biochem 1996;60:1239-42.   DOI
25 Ogunleye A, Bhat A, Irorere VU, Hill D, Williams C, Radecka I. Poly-γ-glutamic acid: production, properties and applications. Microbiology (Reading) 2015;161:1-17.   DOI
26 Yin D, Dong H, Wang TX, Hu ZZ, Cheng NN, Qu WM, Huang ZL. Glutamate activates the histaminergic tuberomammillary nucleus and increases wakefulness in rats. Neuroscience 2019;413:86-98.   DOI
27 Tanimoto H, Fox T, Eagles J, Satoh H, Nozawa H, Okiyama A, Morinaga Y, Fairweather-Tait SJ. Acute effect of poly-gamma-glutamic acid on calcium absorption in post-menopausal women. J Am Coll Nutr 2007;26:645-9.   DOI
28 Jun NR. Effect of poly-gamma glutamic acid, magnesium and vitamin B6 supplementation on metabolic and inflammatory biomarkers and sleep status in caffeine intake rat [dissertation]. Seoul: Kookmin University; 2017.
29 Ciranna L. Serotonin as a modulator of glutamate- and GABA-mediated neurotransmission: implications in physiological functions and in pathology. Curr Neuropharmacol 2006;4:101-14.   DOI
30 Lopez-Rodriguez F, Medina-Ceja L, Wilson CL, Jhung D, Morales-Villagran A. Changes in extracellular glutamate levels in rat orbitofrontal cortex during sleep and wakefulness. Arch Med Res 2007;38:52-5.   DOI
31 Messripour M, Mesripour A. Effects of vitamin B6 on age associated changes of rat brain glutamate decarboxylase activity. Afr J Pharm Pharmacol 2011;5:454-6.   DOI
32 Almeida MR, Mabasa L, Crane C, Park CS, Venancio VP, Bianchi ML, Antunes LM. Maternal vitamin B6 deficient or supplemented diets on expression of genes related to GABAergic, serotonergic, or glutamatergic pathways in hippocampus of rat dams and their offspring. Mol Nutr Food Res 2016;60:1615-24.   DOI
33 Zhao W, Li Y, Ma W, Ge Y, Huang Y. A study on quality components and sleep-promoting effects of GABA black tea. Food Funct 2015;6:3393-8.   DOI
34 Boonstra E, de Kleijn R, Colzato LS, Alkemade A, Forstmann BU, Nieuwenhuis S. Neurotransmitters as food supplements: the effects of GABA on brain and behavior. Front Psychol 2015;6:1520.   DOI
35 Knudsen GM, Poulsen HE, Paulson OB. Blood-brain barrier permeability in galactosamine-induced hepatic encephalopathy. No evidence for increased GABA-transport. J Hepatol 1988;6:187-92.   DOI
36 Mabunga DF, Gonzales EL, Kim HJ, Choung SY. Treatment of GABA from fermented rice germ ameliorates caffeine-induced sleep disturbance in mice. Biomol Ther (Seoul) 2015;23:268-74.   DOI
37 Jin HE, Choi JC, Lim YT, Sung MH. Prebiotic effects of poly-gamma-glutamate on bacterial flora in murine gut. J Microbiol Biotechnol 2017;27:412-5.   DOI
38 Hong KB, Park Y, Suh HJ. Sleep-promoting effects of a GABA/5-HTP mixture: Behavioral changes and neuromodulation in an invertebrate model. Life Sci 2016;150:42-9.   DOI