Browse > Article
http://dx.doi.org/10.5713/ajas.2006.500

Effect of Dietary Vitamin E Supplementation on Serum α-Tocopherol and Immune Status of Crossbred Calves  

Samanta, A.K. (Division of Animal Nutrition, SKUAST)
Dass, R.S. (Mineral and Vitamin Nutrition Laboratory, Center of Advanced Studies in Animal Nutrition Indian Veterinary Research Institute)
Rawat, Mayank (Division of Biological Standardization)
Mishra, S.C. (Division of Biological Standardization)
Mehra, U.R. (Mineral and Vitamin Nutrition Laboratory, Center of Advanced Studies in Animal Nutrition Indian Veterinary Research Institute)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.4, 2006 , pp. 500-506 More about this Journal
Abstract
An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum ${\alpha}$-tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL-${\alpha}$-tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum ${\alpha}$-tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum ${\alpha}$-tocopherol concentration (${\mu}g/100ml$) was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the ${\alpha}$-tocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.
Keywords
Vitamin E; Serum ${\alpha}$-Tocopherol; Crossbred Calves; Immune Response; ELISA; LTT;
Citations & Related Records

Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
연도 인용수 순위
1 Bain, R. V. S., M. C. L. DeAlwis, G. R. Carter and B. K. Gupta. 1982. Haemorrhagic septicaemia. FAO, United Nations, No. 33, Rome
2 Coelho, M. B. 1991. Vitamin Stability in Premixes and Feeds. A practical approach. BASF Technical symposium, Bloomington, Minnesota, p. 56
3 Corwin, L. M. and R. K. Gordon. 1982. Vitamin E and immune regulation. Ann. New York Acad. Sci. 393:437   DOI
4 Kahlon, R. S. and Raj Vir Singh. 2004. Effect of alpha tocopherol supplementation on plasma levels of antioxidant vitamins in anestrus buffalo (Bubalus bubalis) Asian-Aust. J. Anim. Sci. 17:1088-1092   DOI
5 Muneer, R., S. Akhtar and H. Afzal. 1994. Evaluation of three oil adjuvant vaccines against P. multocida in buffalo calves. Revue Scientifique at Technique OIE, 13:837-843   DOI
6 NRC. 1989. Nutrient requirements of domestic animals: Nutrient requirements of beef cattle, 6th ed. National Academy of Sciences - National Research Council, Washington, DC
7 NRC. 2001. Nutrient requirements of dairy cattle. Seventh revised ed. National Research Council, Washington, DC
8 Paulson, G. D., G. A. Broderick, C. A. Baumann and A. L. Pope. 1968. Effect of feeding sheep with selenium fortified trace mineralized salt: effect of tocopherol. J. Anim. Sci. 27:195   DOI
9 Rawat, M. and T. N. Jaiswal. 2004. Immunomodulation of water in oil in water (w/o/w), emulsion vaccines of killed pasteurella multocida (P52) whole cell grown under iron-regulated condition. Ind. J. Anim. Sci. 74(7):701-705
10 Reddy, P. G., J. L. Morrill, H. C. Minocha, M. B. Morrill, A. D. Dayton and R. A. Frey. 1986. Effect of supplemental vitamin E on the immune system of calves. J. Dairy Sci. 69:164   DOI   ScienceOn
11 Rivera, J. D., G. C. Duff, M. L. Galyean, D. A. Walker and G. A. Nunnery. 2002. Effect of supplemental vitamin E on performance, health and humoral immune response of beef cattle. J. Anim. Sci. 80:933-941   DOI
12 Siddons, R. C. and C. F. Mills. 1981. Glutathione peroxidase activity and erythrocyte stability in calves differing in selenium and vitamin E status. Br. J. Nutr. 46:345   DOI   ScienceOn
13 Spratt, M. G. and C. C. Kratzing. 1971. The effect of dietary lipid and $\alpha$-tocopherol on RES activity in choline deficiency. J. Reticuloendothel. Soc. 10:319
14 Segagni, E. 1955. Vitamin E effect on vaccination. Minerva Pediat. 7:985-987
15 Frigg, M. and H. E. Gallo-Torres. 1977. Phagocytosis of $\alpha$- tocopheryl nicotinate by the reticulo-endothelial system. A micro-auto radiographic study. Int. J. Vit. Nutr. Res. 47:145
16 Reddy, P. G., J. L. Morrill, H. C. Minocha and J. S. Stevenson. 1987. Vitamin E is immunostimulatory in calves. J. Dairy Sci. 70:993   DOI   ScienceOn
17 Alderson, N. E., G. E. Mitchell, C. O. Little, R. E. Warner and R. E. Tucker. 1971. Preintestinal disappearance of vitamin E in ruminants. J. Nutr. 101:655-660   DOI
18 Tengerdy, R. P., E. Ameghino and H. Riemann. 1991. Serological response of rams to a Brucella ovis vitamin E adjuvant vaccine. Vaccine, 9:273-276   DOI   ScienceOn
19 Tizzard, I. 1995. Immunology, an introduction, 5th edn. W.B. Saunders Co., Philadelphia
20 Cipriano, J. E., J. L. Morrill and N. V. Anderson. 1982. Effect of dietary vitamin E on immune responses of calves. J. Dairy Sci. 65:2357-2365   DOI   ScienceOn
21 Pehrson, B., J. Hakkarainen, K. E. Tornquist and C. Fossum. 1991. Effect of vitamin E supplementation on weight gain, immune competence, and disease incidence in barley fed cattle. J. Dairy Sci. 74:1054-1059   DOI   ScienceOn
22 Walsh, M. D., D. G. Kennedy, E. A. Goodall and S. Kennedy. 1993. Antioxidant enzyme activity in the muscles of calves depleted of vitamin E or selenium or both. Br. J. Nutr. 70:621-630   DOI   ScienceOn
23 Sheffy, B. E. and Alma J. Williams. 1982. Nutrition and the immune response. J. Am. Vet. Med. Assoc. 180:1073
24 Tengerdy, R. P., D. L. Meyer, L. H. Lauerman, D. C. Lueker and C. F. Nockels. 1983. Vitamin E enhanced humoral antibody response to Clostridia perfringens type D in sheep. Br. Vet. J. 139:147-152   DOI
25 Heinzerling, R. H. 1974. The effect of vitamin E on immunity, Ph.D. Dissertation, Colorado State Univ., Fort Collins, USA
26 Afzal, M., R. P. Tengerdy, R. P. Ellis, C. V. Kimberling and C. J. Morris. 1984. Protection of rams against epididymitis by a B. ovis-vitamin E adjuvant vaccine. Vet. Immunol. Immunopathol. 7:293-304   DOI   ScienceOn
27 Chatterjee, P. N., H. Kaur and N. Panda. 2003. Effect of vitamin E supplementation on plasma antioxidant vitamin and immunity status of crossbred cows. Asian-Aust. J. Anim. Sci. 16:1614- 1618   DOI
28 Panganmala, R. V. and D. G. Cornwell. 1982. The effects of vitamin E on arachidonic acid metabolism. Ann N.Y. Acad. Sci. 393:376-90   DOI
29 Snedecor, G. W. and W. G. Cochran. 1980. Statistical methods, 7th Ed. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi
30 Beisel, W. R. 1982. Single nutrient and immunity. Am. J. Clin. Nutr. 35:417   DOI
31 Pati, U. S. 1994. Characterisation of protective antigen of Pasteurella multocida serotype 6B. M.V.Sc. Thesis, Deemed Univ., IVRI, Izatnagar, India
32 Cuesta, P. A., L. R. McDowell, W. E. Kunkle, M. J. Lawman, F. Bullock, A. Drew, N. S. Wilkinson and F. G. Martin. 1996.Mineral status in beef cattle, live weight changes and immune response as related to selenium and vitamin E supplementation. Livestock Research for Rural Development 8:1-7
33 Hill, G. M., R. L. Stuart, P. R. Utley and J. O. Reagan. 1990. Vitamin E effects on finishing steer performance. J. Anim. Sci. 68 (Suppl. 1):557 (Abstr)
34 Hill, G. M., S. E. Williams, S. N. Williams, S. W. Neel and P. R. Utley. 1993. Vitamin E effects on steer performance and color retention of steaks and ground beef. J. Anim. Sci. 71 (Suppl 1):22 (Abstract)
35 McMurray, C. H. and W. J. Blanchflower. 1979. Application of a high performance liquid chromatographic fluorescence method for the rapid determination of $\alpha$-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and High Performance Liquid Chromatography-Ultraviolet detection methods. J. Chromatography 178:525-531   DOI   ScienceOn
36 Rana, R. and N. C. Srivastava. 1999. Immune response in rabbits (Lepus cuniculus) immunized with Mycoplasma mycoides sub sp. Capri in different adjuvants. Ind. J. Vet. Res. 8:45-53
37 Larsen, H. J. and S. Tollersrud. 1981. Effect of dietary vitamin E and selenium on the phytohemagglutinin response of pig lymphocytes. Res. Vet. Sci. 31:301
38 Franchini, A., M. Canti, G. Manfreda and S. Bertuzzi. 1991. Vitamin E as adjuvant in emulsified vaccines for chicks. Poult. Sci. 70:709-713   DOI   ScienceOn
39 Hill, G. A. 1987. Vitamin E and selenium supplementation of cattle. In: Proc. Georgia Nutrition Conference for the Feed Industry. Agriculture Experimental Station, Univ. of Georgia, Athens, pp. 57-65
40 Drugs and Cosmetics Acts and Rules (VI Amendment). 1994. Government of India
41 Mondal, B. C. 1996. Performance of crossbred (Bos indicusx Bos taurus) calves on grain less diet during milk and post milk feeding period. Ph.D. thesis, Deemed Univ. IVRI, Izatnagar, India
42 Reddy, P. G., J. L. Morrill, H. C. Minocha, R. A. Frey, M. B. Morill and A. D. Dayton. 1985. Effect of serum from vitamin E supplemented calves on infectious bovine rhinotrachatis virus replication. J. Dairy Sci. 68(Suppl 1):146
43 Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics .A Biometrical Approach, 2nd ed., McGraw Hill Book Company, New York, USA
44 Orth, C. K. 1992. The glucocorticoids suppress immunity. New Scientists 205:311-313
45 Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival, application to proliferation and cytotoxicity assays. J. Immunological Methods 65:55-63   DOI   ScienceOn
46 Almedia, J. D., P. S. Atanasiu, J. E. Gerdner, A. W. Schuurs, A. Vollar and R. H. Yolken. 1979. Manual for laboratory viral diagnosis. WHO offset publication No. 47, WHO, Geneva
47 Lowry, O. H., N. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the folin phenol reagents. J. Biol. Chem. 193:265-275