• Food Science and Preservation
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• v.19 no.1
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• pp.62-66
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• 2012

With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.1-6
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• 2004

$\beta$-Sitosterol is the major phytosterol in higher plants, including fruits and vegetables. The molecule has been shown to have the potential for prevention and therapy for human cancer. We investigated the effects of $\beta$-sitosterol on the cell proliferation of HCT116 human colon cancer cells in order to understand its anti-proliferative mechanism. $\beta$-Sitosterol treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. The anti-proliferative effect of HCT116 cells by $\beta$-sitosterol was associated with formation of apoptotic bodies and degradation of $\beta$-catenin protein. In addition, $\beta$-sitosterol-treatment induced a marked accumulation of tumor suppressor p53 and a concomitant induction of cyclin-dependent kinase (Cdk) inhibitor p21 without alteration in the levels of cyclins and Cdks. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of $\beta$-sitosterol.

• Journal of Dairy Science and Biotechnology
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• v.31 no.2
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• pp.153-159
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• 2013

Lactic acid bacteria are microorganisms that are closely associated with human and/or animal environments, and are categorized as generally recognized as safe (GRAS) organisms due to their ubiquitous appearance in foods and their contribution to the healthy microflora of mucosal surfaces. This study was performed to isolate and identify lactic acid bacteria with antagonistic effects against food-borne pathogens. A total of 3,000 acid-producing bacteria were isolated from infant feces, cattle feces, goat feces, dog feces, pig feces, vaginal tracts, vegetables, fruits, Kimchi, Jeotgal, fermented sausages, raw milk, cheese, yogurt, Cheonggukjang, Meju, and Makgeolli cultured on MRS agar with 0.05% bromocresol purple. For the isolation of bacteriocin-producing bacteria, the diameter of the clear zone was measured on MRS agar plates. Twenty-six isolates exhibited strong antibacterial activity against indicator strains such as Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica serovar Enteritidis. Lactic acid bacteria were identified as Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus curvatus, Lactobacillus plantarum, and Pediococcus acidilactici by 16S rDNA gene sequence analysis. The results of this study suggest that the isolates could be used as potential probiotic starters for functional food applications.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.440-448
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• 2013

The purpose of this study was to analyze the effects of perceived stress on dietary habits and oral health behaviors in Korean adolescents. Data were from the Korea Youth Risk Behavior Web-based Survey (KYRBS) in 2011. A total of 74,186 adolescents were surveyed using the self-administered questionnaire. Logistic regression analyses were performed to elucidate the effects of perceived stress on dietary habits and oral health behaviors. Statistical analyses were conducted, and p<0.05 were considered significant. We found that adolescents with higher stress were more likely to increase the risk of adverse dietary habits (low consumption of fruits, vegetables and milk, high consumption of carbonated soft drinks, fast food and cookie) compared to those with lower stress. Regarding the relationship between perceived stress and oral health behaviors, adolescents with higher stress were less likely to practice oral health behaviors such as brushing teeth after snack consumption and brushing teeth before sleep compared to those with lower stress. This result indicates that adolescents' perceived stress might play a significant role in the negative dietary habits and oral health behavior.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.417-433
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• 2011

The study tested QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation and HPLC-MS/ MS analysis for measurement of pesticide residues in fruit and vegetable. 240 kinds of pesticides spiked at three levels of 90, 45, 9 ng/g in lettuce and apple. For QuEChERS sample preparation, graphitized carbon black (GCB) was used for only lettuce in dispersive-SPE as absorbent. Matrix-matched standard calibration was used for quantitative analysis of HPLC-MS/MS. 218 pesticides (91%) in apple and 207 pesticides (86%) in lettuce showed recoveries in the range of 70~120% with $RSD{\leq}20%$. The lowest calibrated level (LCL) were 4.5 ng/g for 192 pesticides, 9 ng/g for 42 pesticides, 45 ng/g for 3 pesticides and 3 pesticides were not detected at all concentration levels. The results showed that the QuEChERS sample preparation and HPLC-MS/MS analysis can be applied to multi-residue analysis of pesticides in vegetables and fruits.

• Food Science and Preservation
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• v.15 no.3
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• pp.377-384
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• 2008

The gamma irradiation process was performed to prolong a shelf-life of peaches and the effects of a gamma irradiation on the nutritional, physiochemical and sensory characteristics of peaches were evaluated during a post-irradiation storage at $4^{\circ}C$ and $25^{\circ}C$. Nutritional, physiochemical and sensory characteristics of peaches were stable at 1 kGy of an irradiation, a recommended dose for fruits and vegetables by CODEX, and the viable cell counts of contaminated microorganisms were reduced by 2 decimal reduction, at this dose. After a 1-week storage at ambient condition, the microbiological quality of the 1 kGy irradiated peach was stable, while all the non-irradiated peaches were deteriorated Furthermore, cold storage enhanced the shelf-life of the gamma irradiated peach and the nutritional and physiochemical characteristics of the peaches were comparatively stable up to 6 weeks. Right after gamma irradiation, the sensory evaluation results were not different in any of the samples, and the sensory quality of the irradiated peaches was adequate for a 4-week storage at $4^{\circ}C$. Our results suggested that gamma irradiation at 1 kGy can be used to enhance the shelf-life of peach without a significant loss in the quality attributes, especially upon cold storage after radiation treatment.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.395-401
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• 2008

Flavonoids are ubiquitous substances in fruits and vegetables. A main subgroup of the flavonoids are the flavonols, of which quercetin and kaempferol are the major representatives in foods. They are used in food supplements at high doses, because of their preventive effects on degenerative diseases. The aim of this study was to determine the combined and separate effects of kaempferol and quercetin on oxidative stress in cow pulmonary artery endothelium (CPAE) cells over a broad concentration range. The results demonstrate that cell viability was greatly increased in kaempferol and quercetin treated cells whether $H_{2}O_{2}$-treated or not. Cell viability also increased when treated with flavonols in the absence of oxidative stress. Both preincubation and simultaneous incubation with kaempferol and quercetin protected against the loss of cell viability induced by 1mM $H_{2}O_{2}(5h)$. Protective effects of flavonols against oxidative stress were shown to depend on the treated flavonol concentrations. No protective effect was shown under low concentration treatment and cell viability increased 1.6 times at $200{\mu}M$ relative to the control group. At the highest flavonol concentration of $300{\mu}M$, the increased cell viability by flavonol treatment was decreased to almost half of the maximum values. Combined treatments with kaempferol and quercetin showed more protective effects against oxidative stress by $H_{2}O_{2}$ than the separate effects of each flavonol. In conclusion, the protective effect of kaempferol and quercetin against oxidative stress was very pronounced but high concentrations of flavonols can also induce cell cytotoxicity.

• Archives of design research
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• v.17 no.1
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• pp.25-36
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• 2004

The purpose of this study is to analyse and interpret the way of differentiated expression and changing creative trend following 5-day workweek which has been recently adopted. For this aim 11 pieces of distinctive advertising text were analysed. The criteria for selection of analysing text were relativeness with 5-day workweek and cognitive inconsistency. These texts were negative phrases, exaggerated expression, and excessively humorous appeals. The study revealed the followings: First, 5-day workweek concerned advertising texts were considered as high attention getting and successful in stimulating advertising audiences' curiosity by using the elements opposing the value and belief system formed in the existing audience. Second, consumption-driving lifestyle was found in most expressions for credit cards advertising. Third, hedonic need-oriented lifestyle was typical in young consumer products category. Fourth, family centered lifestyle emphasized meaning of rest and solidarity of family.

• Korean Journal of Food Science and Technology
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• v.4 no.2
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• pp.140-150
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• 1972

Using methods of principle component analysis and pattern of analysis. Korean food supply pattern and its change (1962-1969) have been compared whit those of other 40 countries. 1) Among the 4 groups classified on the first and second principle components, Korea belongs to the highest crop grain dependent group of Asia. Although Korea is identified as one of the highest crop consuming countries among the group, a significant increase in the consumption of fruits and vegetables is noticed during the period. 2) According to the pattern of numerics analysis Korean food supply pattern (1969) is similar with those of Japan (1949), philippines, Taiwan, while is quite apart from those of U.S.A., W. Germany, France and Australia. 3) During the period the food supply pattern of Korea has been changed significantly toward the more common locations on the world food supply pattern map. Yet the location is far from the desirable one and its moving direction is characteristic, differing not only those of Europian countries but also Asian neighborhoods. this unique pattern should receive a careful examination and thus proper measures should be undertaken to take care of optimal nation's nutritional requirements.

• Journal of Korean Home Economics Education Association
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• v.24 no.4
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• pp.91-104
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• 2012

The purpose of this study was to examine a questionnaire survey on nutrition knowledge, dietary behaviors and the evaluation of nutrient intakes in regard to frequency of eating breakfast for a total 383 high school female students in Chuncheon area. The subjects were categorized into 3 groups according to frequency of breakfast : eating breakfast group (6-7 times/week, 49.1%), sometimes eating breakfast group (3-5 times/week, 22.2%), skipping breakfast group (${\leq}2$ times/week, 28.7%). The eating breakfast group and sometimes eating breakfast group have better grades on nutrition knowledge related to breakfast than the skipping breakfast group. The skipping breakfast group have a smattering of nutrition knowledge like, the importance of having breakfast, maintaining calories, and the brain's energy resource. There was a strong correlation between the frequency of breakfast and dietary behavior grades. The students who skip breakfast have irregular eating habits and their eating speed was improper. Also, their frequency of taking in cereals, proteins, fruits, vegetables, and dairy products was poor. The results of the survey on nutrition intake show that while, students who eat breakfast are consuming only folate, calcium and iron at a less than recommend intake, the students who skip breakfast are consuming vitamin $B_2$, niacin, vitamin C, vitamin $B_1$ as well as folate, calcium, and also iron at a less than recommended intake. Based on these results, it is necessary to develop an awareness program that emphasizes the importance of breakfast and accurate nutrition knowledge for students.