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http://dx.doi.org/10.3746/jkfn.2004.33.1.001

β-Sitosterol Induced Growth Inhibition is Associated with Up-regulation of Cdk Inhibitor p21WAF1/CIP1 in Human Colon Cancer Cells  

Choi, Yung-Hyun (Dept. of Biochemistry, College of Oriental Medicine, Dongeui University)
Kim, Young-Ae (Dept. of Food Science and Nutrition, Pusan National University)
Park, Cheol (Dept. of Biology, Pusan National University)
Choi, Byung-Tae (Dept. of Anatomy, College of Oriental Medicine, Dongeui University)
Lee, Won-Ho (Dept. of Biology, Pusan National University)
Hwang, Kyung-Mi (Dept. of Food Science and Nutrition, Pusan National University)
Jung, Keun-Ok (Dept. of Food Science and Nutrition, Pusan National University)
Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.1, 2004 , pp. 1-6 More about this Journal
Abstract
$\beta$-Sitosterol is the major phytosterol in higher plants, including fruits and vegetables. The molecule has been shown to have the potential for prevention and therapy for human cancer. We investigated the effects of $\beta$-sitosterol on the cell proliferation of HCT116 human colon cancer cells in order to understand its anti-proliferative mechanism. $\beta$-Sitosterol treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. The anti-proliferative effect of HCT116 cells by $\beta$-sitosterol was associated with formation of apoptotic bodies and degradation of $\beta$-catenin protein. In addition, $\beta$-sitosterol-treatment induced a marked accumulation of tumor suppressor p53 and a concomitant induction of cyclin-dependent kinase (Cdk) inhibitor p21 without alteration in the levels of cyclins and Cdks. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of $\beta$-sitosterol.
Keywords
$\beta$-sitosterol; cell cycle; p53; p21;
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