• Title/Summary/Keyword: vegetable structure

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Ultrastructural Aspects of the Mixed Infections of Watermelon Mosaic Potyvirus and Cucumber Green Mottle Mosaic Tobamovirus Isolated from Watermelon

  • Kim, Jeong-Soo;Cho, Jeom-Deog;Park, Hong-Soo;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.211-215
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    • 2000
  • Symptoms on 4 varieties of watermelons inoculated with watermelon mosaic potyvirus II isolated from watermelon (WMV-W) were severe mosaic and leaf malformation while those inoculated with cucumber green mottle mosaic tobamovirus from watermelon (CGMMV-W) were mild mosaic and chlorotic spots. Inoculation of the mixture of WMV-W and CGMMV-W produced extremely severe mosaic along with necrotic spots and general necrosis. Doubly infected plants were also stunted. Cells infected with WMV-W or CGMMV-W alone exhibited the intrinsically ultra-structural properties of each virus infection. WMV-W induced potyvirus-characteristic cylindrical inclusions in the cytosol. Virus particles were orderly aligned along the tonoplasts. CGMMV-W induced tobamovirus-characteristic stacked crystalline arrays of virus particles in the cytosol. Cells infected doubly with WMV-W and CGMMV-W contained striking cytopathic effects that were not present in single infection of each virus. The unique ring structure, nonagon, was that a single potyvirus particle was surrounded by 9 CGMMV-W tobamovirus particles.

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Solvent-free, Soap-free Synthesis Process of Methyl Fructoside Oleic Acid Polyester (무용매 , 무유화제 공정에 의한 메틸프룩토시드 올레산 폴리에스테르의 합성)

  • Heo, Joo-Hyung;Kim, Chong-Tai;Kim, Hae-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.15 no.4
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    • pp.45-56
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    • 1998
  • Methyl fructoside oleic acid polyester(MFPE), fructose-based sugar polyester, was synthesized by solvent-free, soap-free transesterification of methyl oleate with methyl fructoside(MF) as a sugar starting material in the presence of conventional potassium carbonate basic catalyst. Methyl fructoside was found to be an effective sugar starting material, because of its low softning point, high heat stability, high miscibility, and high reactivity than other sugars. Yield 98% of purified MFPE based on initial weight of MF was obtained at 1:5 of the molar ratio of methyl fructoside to methyl oleate, 2%(w/w) of potassium carbonate catalyst content, 20${\sim}$200mmHg of reduced pressure and $180^{\circ}C$ of reaction temperature. MFPE structure was confirmed by infrared and proton nuclear magnetic resonance spectroscopy. Physical properties of methyl of fructoside oleic acid polyester such as viscosity, HLB, solubility, color, refractive index, specific gravity, and density were similar to physical properties of sucrose polyesters(SPE) and vegetable oils. Then, it was elucidated that MFPE was sufficient to replace the SPE and conventional oils.

Lubricity Characterization of Hydrogenated Biodiesel as an Alternative Diesel Fuel (경유 대체연료로서 수첨바이오디젤의 윤활 특성 연구)

  • Kim, Jae-Kon;Jeon, Cheol-Hwan;Yim, Eui-Soon;Chung, Choong-Sub
    • Tribology and Lubricants
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    • v.28 no.6
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    • pp.321-327
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    • 2012
  • Paraffin bio-based hydrotreated biodiesel(HBD) is originated from vegetable oil(the process can also be applied to animal fat) with the the chemical structure $C_nH_{2n+2}$. In the number of process of the oil or fat, the hydrogenation is significantly important to create a bio-based diesel fuel. This study is focused on lubricity characteristics of BTL diesel blends to use alternative diesel fuel in Korea. The BTL diesel are blended the different volume ratios (HBD 5(5 vol.% HBD - 95 vol.% diesel), HBD 10, HBD 20, HBD 30, HBD 40 and HBD 50. HBD with paraffin compounds showed a very high centane number, low sulfur content and free aromatic compound. Especially, the wear scar of HBD showed poor lubricity compared to automotive diesel due to the fuel composition, low sulfur content and free aromatic compound. Also, the lubricity specification of automotive diesel with different six HBD blends is within the limit by the Korean standards. Finally, HBD as an alternative diesel fuel is challengeable in transportation sector of Korea.

A Food and Nutrition System Analysis of South Korea

  • Jeffery Sobal;Lee, Soo-Kyung
    • Journal of Community Nutrition
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    • v.5 no.4
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    • pp.209-217
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    • 2003
  • The food and nutrition system is a network of processes linking agriculture, food, eating, nutrition, and health. The system includes a series of nine stages (production, processing, distribution, acquisition, preparation, consumption, digestion, transport, and utilization) and two types of contexts (biophysical and sociocultural). Analysis of whole food and nutrition systems provides information about the structure and processes involved in the complete scope of food and nutrition, assisting in the identification of ″upstream″ influences and ″downstream″ consequences in the system. The current analysis gathered existing data about the food and nutrition system in South Korea from public sources and professional publications, and interpreted that information to consider how different elements of the system contribute to health. The findings revealed that South Korea has substantial domestic food production and processing supplemented by imports, widespread food distribution in markets and a growing number of Korean and Western restaurants, a relatively low percentage of household income spent on food, growing use of new food preparation methods such as microwave ovens, a rice/vegetable/fish based three-meal consumption pattern, few recorded digestive problems, increasing calorie storage as body fat, and a relatively low chronic disease prevalence compared to other developed societies. Examination of the full scope of the food and nutrition system provides a broad perspective using whole system thinking that can identify potential strategies for future research and intervention.

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80

  • Chiewpattanakul, Paramaporn;Phonnok, Sirinet;Durand, Alain;Marie, Emmanuelle;Thanomsub, Benjamas Wongsatayanon
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1664-1671
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    • 2010
  • A new biosurfactant producer was isolated from palm-oil-contaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% $NH_4NO_3$, at a pH of 5.3, with shaking at 200 rpm, and a temperature of $30^{\circ}C$ for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.

Transesterification Using the Cross-Linked Enzyme Aggregate of Photobacterium lipolyticum Lipase M37

  • Han, Jin-Yee;Kim, Hyung-Kwoun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.11
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    • pp.1159-1165
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    • 2011
  • Biodiesel is methyl and ethyl esters of long-chain fatty acids produced from vegetable oils or animal fats. Lipase enzymes have occasionally been used for the production of this biofuel. Recently, biodiesel production using immobilized lipase has received increased attention. Through enhanced stability and reusability, immobilized lipase can contribute to the reduction of the costs inherent to biodiesel production. In this study, methanol-tolerant lipase M37 from Photobacterium lipolyticum was immobilized using the cross-linked enzyme aggregate (CLEA) method. Lipase M37 has a high lysine content (9.7%) in its protein sequence. Most lysine residues are located evenly over the surface of the protein, except for the lid structure region, which makes the CLEA preparation yield quite high (~93%). CLEA M37 evidences an optimal temperature of $30^{\circ}C$, and an optimal pH of 9-10. It was stable up to $50^{\circ}C$ and in a pH range of 4.0-11.0. Both soluble M37 and CLEA M37 were stable in the presence of high concentrations of methanol, ethanol, 1-propanol, and n-butanol. That is, their activities were maintained at solvent concentrations above 10% (v/v). CLEA M37 could produce biodiesel from olive oil and alcohols such as methanol and ethanol. Additionally, CLEA M37 generated biodiesel via both 2-step methanol feeding procedures. Considering its physical stability and reusability, CLEA M37 may potentially be used as a catalyst in organic synthesis, including the biodiesel production reaction.

A Study on Heating Characteristics of Li-ion Battery Applicated Single-phase Immersion Cooling Technology (단상계 침지냉각 기술이 적용된 Li-ion계 배터리 발열특성에 관한 연구)

  • Kim, Woonhak;Kang, Seokwon;Shin, Giseok
    • Journal of the Society of Disaster Information
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    • v.18 no.1
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    • pp.163-172
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    • 2022
  • Purpose: To secure efficient thermal management technology for Li-ion batteries, the applicability of the system applied with single-phase immersion technology was checked through an experiment. Method: Using JH3 pouch cells produced by LG-Chem, Korea, A 14S2P module was manufactured and immersed in a vegetable-based cooling fluid produced by Cargill, USA, and then charged and discharged at a rate of 0.3C to 1C to check the heat distribution. Result: It was possible to manage and there was no change in the molecular structure of the immersion solution. Conclusion: It was confirmed that the immersion cooling method can be applied to the thermal management of Li-ion batteries.

Inheritance of Shoot-Lacking Phenomenon in Interspecific Hybrids of Squash (호박 종간잡종에서 생장점 결여현상의 유전)

  • Hong, K.H.;Huh, Y.C.;Woo, Y.H.;Lee, K.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.12 no.1
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    • pp.47-52
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    • 2010
  • Squash cultivars of Cucurbita moschata have good fresh quality, but their viny growth habit hinders wide cultivation under structure. On the other hand, 'Zucchini' varieties of C. pepo have bush growth habit and better growth ability under the low temperature condition than C. moschata, but have inferior flesh quality. To combine desirable characteristics of the two species, many attempts have been exerted to obtain interspecific hybrids. A shootless phenomenon was found among segregating populations; the cotyledon expanded normally but further shoot growth. The inheritance of the character was investigated in F2 and BC1F2 populations. The segregation ratios indicated that the shoot-lacking characteristics is expressed by double recessive gene pairs.

Genetic Diversity and Population Structure of Korean Soybean Collection Using 75 Microsatellite Markers

  • Lee, Gi-An;Choi, Yu-Mi;Yi, Jung-Yoon;Chung, Jong-Wook;Lee, Myung-Chul;Ma, Kyung-Ho;Lee, Sok-Young;Cho, Jin-Woong;Lee, Jung-Ro
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.492-497
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    • 2014
  • Soybean (Glycine max L.) is crucial legume crop as source of high quality vegetable protein and oil, and Korea is regarded as a part of center of soybean origin. To expand the information of conserved genetic diversity, we analyzed the genetic variability of soybean collection mainly introduced Korean accessions using 75 microsatellite markers. A total of 1,503 alleles with an average value of 20.0 alleles were detected among 644 accessions. Korean collection revealed average allele number of 13.4 while Chinese, Japanese and Southeast Asian accessions showed 9.0, 5.4 and 6.5 mean alleles, respectively. Especially, Korean accessions showed more number of private allele per locus as 3.4 contrary to other geographical groups. The mean expected heterozygosity and polymorphic information content was 0.654 and 0.616, respectively, and expected heterozygosity values were not significantly distinguished according to the geographical groups. The phylogenetic dendrogram and deduced population structure based on DNA profiles of 75 SSR loci showed Korean accessions formed distinct gene pool against Chinese accessions, and could be divided into five subpopulations. Korean soybean accessions have specific genetic diversity and might be serve the valuable alleles for bio-industry as a part of the center of soybean origin.

Characterization of Acid- and Pepsin-soluble Collagens from Rockfish Sebastes schlegeli Skin

  • Kim, Hyung-Jun;Jee, Seong-Joon;Yoon, Min-Suck;Youn, Mu-Ho;Kang, Kyung-Tae;Lee, Dong-Ho;Heu, Min-Soo;Kim, Jin-Soo
    • Fisheries and Aquatic Sciences
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    • v.12 no.1
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    • pp.6-15
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    • 2009
  • Biochemical and functional properties of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from rockfish skin were characterized. Yield of PSC (90.0%) was higher than that of ASC (63.2%). Both ASC and the PSC consisted of ${\alpha}1$ and ${\alpha}2$ chains, and $\alpha$-cross-linked components. According to the results of hydroxylation of proline and lysine, and FT-IR, no difference between the helical structure of ASC and PSC was identified. Thermal denaturation temperature (TDT) of ASC from rockfish skin was $22.8^{\circ}C$, the same as exhibited in PSC. Both ASC and PSC were higher in water absorption capacity (WAC) and oil absorption capacity (OAC) than other vegetable proteins. According to the results of emulsifying activity (EA) and cooking stability (CS), both ASC and PSC from rockfish skin were inferior compared to the commercial emulsifier (Tween-80). The results of FT-IR suggested that the structure of PSC was slightly different when compared to that of ASC. No differences in solubility were established between ASC and PSC from rockfish skin at various pH and NaCl concentrations.