• The Plant Pathology Journal
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• v.35 no.3
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• pp.257-273
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• 2019

Tomato (Solanum lycopersicum) is one of the most widely grown and economically important vegetable crops in the world. Tomato chlorosis virus (ToCV) is one of the recently emerged viruses of tomato distributed worldwide. ToCV-tomato interaction was investigated at the molecular level for determining changes in the expression of tomato genes in response to ToCV infection in this study. A cDNA library enriched with genes induced in response to ToCV infection were constructed and 240 cDNAs were sequenced from this library. The macroarray analysis of 108 cDNAs revealed that the expression of 92 non-redundant tomato genes was induced by 1.5-fold or greater in response to ToCV infection. The majority of ToCV-induced genes identified in this study were associated with a variety of cellular functions including transcription, defense and defense signaling, metabolism, energy, transport facilitation, protein synthesis and fate and cellular biogenesis. Twenty ToCV-induced genes from different functional groups were selected and induction of 19 of these genes in response to ToCV infection was validated by RT-qPCR assay. Finally, the expression of 6 selected genes was analyzed in different stages of ToCV infection from 0 to 45 dpi. While the expression of three of these genes was only induced by ToCV infection, others were induced both by ToCV infection and wounding. The result showed that ToCV induced the basic defense response and activated the defense signaling in tomato plants at different stages of the infection. Functions of these defense related genes and their potential roles in disease development and resistance to ToCV are also discussed.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.643-653
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• 2022

This study investigated which nutrient solution is suitable for growth and secondary metabolite contents when three different vegetable plants are grown simultaneously in one hydroponic cultivation bed. Seeds of pak choi (Brassica compestris L. ssp chinsensis), red mustard (Brassica juncea L.), and arugula (Eruca sativa Mill.) were sown in the shape of a triangle in three places on rockwool cubes. The rockwool cubes were placed in semi deepflow technique (semi-DFT) hydroponic systems in a rooftop greenhouse after three weeks of growth as seedlings then cultivated with four different nutrient solutions, Korea Horticultural Experiment Station (KHE), Hoagland, Otsuka-A, and Yamazaki, at the rooftop greenhouse for two weeks. The leaf area of pak choi cultivated in Otsuka-A was the largest but SPAD values, leaf area, and fresh weight of arugula were highest with KHE treatment. The total glucosinolate (GSL) content of pak choi was 151.7% higher in KHE than in Hoagland, and there was no significant difference in Yamazaki and Otsuka-A treatments. The total GSL content of red mustard was 34.6 μmol·g^-1 in Hoagland, and it was 32.6% higher in Hoagland than in Yamazaki. Total GSL content of arugula was 57.5% higher in Yamazaki and Hoagland nutrients than in KHE and Otsuka-A nutrients solutions. The total GSL content of three plants grown with KHE was 40.7% higher than with Yamazaki, and the other nutrient solutions did not show significant differences. Therefore, KHE nutrient solution is considered suitable for nutrient solution composition for the cultivation of three different Brassicaceae crops in a single hydroponic cultivation system.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.419-427
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• 2021

Due to the effects of climate change and the reduction of the labor force due to COVID-19, the crop yield, harvest time, and cultivated area are rapidly changing every year. In order to respond flexibly to this situation, attempts to apply smart farm technology based on ICT (Information and Communication Technology) to individual farms are increasing. On the other hand, various stakeholders are trying to predict the yield of crops using artificial intelligence and IoT technology, but accurate prediction is difficult due to the lack of learning data. In this study, in order to overcome the data collection problem limited to a specific institution, a smart farmer service technology based on community mapping was developed in which farmers directly participate, input and share accurate data to predict production. In the process, analysis was performed on napa cabbage, which is a vegetable with a large price change compared to production.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.40-40
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• 2023

Atopic dermatitis is a chronic inflammatory skin diseases caused by skin barrier dysfunction. Allium victoralis var. Platyphyllum (AVP) is a perennial plant used as vegetable and herbal medicine. The purpose of this study was to suggest that AVP is a new cosmetic material by examining the effects of AVP on the skin barrier and inflammatory response. A bibliometric network analysis was performed through keyword co-occurrence analysis by extracting author keyword from 69 articles retrieved from SCOPUS. We noted the anti-inflammatory activity shown by the results of clustering and mapping from network visualization analysis using VOSviewer software tool. HPLC-UV analysis showed that AVP contains 0.12 ± 0.02 mg/g of chlorogenic acid and 0.10 ± 0.01 mg/g of gallic acid. AVP at 100 ㎍/mL was shown to increase the mRNA levels of filaggrin and involucrin related to skin barrier function by 1.50-fold and 1.43-fold, respectively. In the scratch assay, AVP at concentrations of 100 ㎍/mL and 200 ㎍/mL significantly increased the cell migration rate and narrowed the scratch area. In addition, AVP suppressed the increase of inflammation-related factors COX-2 and NO and decreased the release of β-hexosaminidase. This study suggests that AVP can be developed as a functional cosmetic material for atopy management through skin barrier protection effects, anti-inflammatory and anti-itch effects.

• Journal of Ecology and Environment
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• v.48 no.1
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• pp.60-73
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• 2024

Background: The available information on terrestrial pest gastropods and their impact on the environment worldwide is scarce and outdated. The present study aimed to address this gap by conducting the first comprehensive survey of pest gastropods in the Nuwara Eliya District, an important vegetable growing area in the highlands of Sri Lanka. Eighty agricultural lands were surveyed over two years by establishing ten 1 m2 sampling plots per crop type in each agricultural land. Geo-coordinates, air temperature, elevation, relative humidity, daily rainfall, soil pH, species richness and abundance were recorded for rainy and non-rainy periods. The relationship between species composition and environmental variables was analyzed using multi-regression models and distribution maps. Results: Out of the 14 species recorded in agricultural lands, nine were identified as exotic pest species. Species abundance (t = 4.69, p < 0.05) and diversity was higher in the rainy period and the dominant species during this period were Bradybaena similaris (t = 2.69, p < 0.05) and Deroceras reticulatum (t = 2. 46, p < 0.05). Eggs and estivating adults were found in soil and under decaying organic matter during the non-rainy period. The exotic species showed broader preferences for the measured environmental factors and showed a wider range in distribution compared to the native species. Variation in pest gastropod composition was significantly accounted for by elevation, relative humidity, soil pH and daily rainfall. Additionally, the species richness and abundance varied across locations due to the combined effects of elevation, crop type and stage, and field type. Conclusions: The study emphasizes the importance of understanding the biology and ecology of gastropod pests to develop effective management strategies. By considering the influence of environmental factors and implementing appropriate soil management techniques, such as targeting specific habitats and crop stages, it is possible to mitigate pest populations and minimize their impact on agricultural lands. Overall, this research contributes valuable insights into the dynamics and interactions of terrestrial gastropods in agricultural ecosystems, supporting sustainable pest management practices.

• The Plant Pathology Journal
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• v.40 no.4
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• pp.390-398
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• 2024

The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.

• Journal of Korean Society for Quality Management
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• v.52 no.2
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• pp.201-220
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• 2024

Purpose: Smart farming is related to the low carbon certification system as it provides many opportunities to cultivate and manage crops in an eco-friendly, thereby reducing carbon footprint. However, there is a significant lack of consumer perception research on low carbon labels for smart farms vegetables. Therefore, this study aims to investigate consumer perceptions of smart farm vegetable and low carbon labels. Methods: This study manipulated cultivation type(general vs. smart farm) and low carbon labels (yes vs. no) as experimental stimuli. Measurement questions and the research model were validated through confirmatory factor analysis and reliability analysis. Hypotheses testing were conducted using SPSS 29.0, AMOS 28.0. Results: The results of the study showed no significant difference in consumers perceived naturalness based on cultivation types, and there was also no moderating effect of the low carbon label. There was no difference between environmental benefits and health benefits according to the cultivation type. Perceived naturalness had a significant effect on both environmental and health benefits, and environmental benefits showed a higher impact relationship. These benefits positively affected attitudes and willingness to pay a premium, Environmental benefits had a higher impact on attitudes, while health benefits had a higher impact on willingness to pay a premium. Lastly, attitudes were found to have a significant impact on the willingness to pay a premium. Conclusion: This study is valuable in that it investigated consumer perceptions of smart farms and low carbon labels that have not been previously studied. It compares the environmental and health benefits, confirming their influence on attitudes and willingness to pay a premium. The results suggest a potential expansion in academic research on smart farming and low carbon labels, offering practical insights for marketing strategies and policies for relevant companies.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.2
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• pp.93-102
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• 1993

Carbonized rice hull, neutralized by dilute nitric acid, was evaluated possibility as a bed matrial for sanitary cultivation. The growth response of Chinese Cabbage, lettuce, and spinach on the carbonized rice hull supplemented with different kinds and concentrations of available nutrition solution was accessed. The ideal nitrogen concentration of nutrition solution was 126 mg/l. Both solutions of compound fertilizer and nutrition containing microelements showed no difference in growth and chemical components of vegetables. Therefore, compound fertilizer was thought to be better than nutrition owing to the convenience of handling in practice. The gravel was also evaluated as supporting material of carbonized rice hull. Because of lasting latent heat in gravel, the mixing treatment of carbonized rice hull and gravel(7~10cm in diam.) was efficient to the growth resulting in the highest dry weight per plant, but the heavy weight of gravel made the handling very difficult. Light carbonized rice hull showed the better plant growth and ease handling, compared to the mixture of soil and compost, and had enough supporting capacity. Therefore, carbonized rice hull was thought to be a desirable bed material for environmentally controlled cultivation.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.657-663
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• 2012

The Ca deficiency is a serious problem in the propagation of domestically bred 'Seolhyang' strawberry through soil cultivation. The objective of this research was to investigate the impact of the Ca containing fertilizers on the decrease of Ca deficiency symptoms and in the growth of mother and daughter plants. To achieve this, chemicals of 1.125 mM $Ca(OH)_2$, 0.375 mM $MgCl_2$, and 1.25 mM KCl were blended to contain the K:Ca:Mg (4:2:1) and the influence was compared to a commercial fertilizer, Azuro-Calma ($NO_3$-N 13%, $K_2O$ 1%, CaO 16%, MgO 6%), when those were applied as solutions with the electrical conductivity (EC) controlled to 0.6 or 1.0dS ${\cdot}m^{-1}$. During 120 days after transplant, the Azuro-Calma was more effective than the combined fertilizer in the reduction of mother and daughter plants on which Ca deficiency was appeared. The application of two Ca containing fertilizers resulted in the heavier fresh and dry weights of mother plants. The treatments of 1.0dS ${\cdot}m^{-1}$ of two fertilizers were more effective than those of 0.6dS ${\cdot}m^{-1}$. The combined fertilizer was also more effective than Azuro-calma on the growth of above ground plant tissue. The results in length, fresh and dry weight of runners occurred from a mother plants showed that the treatment of 1.0dS ${\cdot}m^{-1}$ of combined fertilizer was most effective followed by those of 1.0dS ${\cdot}m^{-1}$ of Azuro-Calma, 0.6dS ${\cdot}m^{-1}$ of combined fertilizer, 0.6dS ${\cdot}m^{-1}$ of Azuro-Calma, and control. The fresh weights of daughter plants were heavier in the treatments of Ca application than those in the control treatment, but the differences between 0.6dS ${\cdot}m^{-1}$ and control were not significant. The above results indicate that Azuro-Calma is more effective in decreasing plants showing the Ca deficiency symptoms. However, the combined fertilizer of K:Ca:Mg is more desirable when we are concerned about the decrease of crops showing Ca deficiency as well as increase of the growth in above ground plant tissue.