• Archives of Plastic Surgery
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• v.34 no.6
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• pp.679-684
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• 2007

Purpose: DMH(1,2-dimethylhydrazine) has been known to induce vascular neoplasm such as malignant endothelioma in animal experiment, through induction of abnormal proliferation of HUVECs. In our previous studies, 11 types of PKC isoenzymes were determined by RT-PCR and the expression of $PKC{\alpha}$, and ${\mu}$ was more prominent than other PKC isoenzymes in the DMH-treated group. However, this result was not based on objective assessment. In this study, we further evaluated the role of $PKC{\alpha}$ on the DMH-induced abnormal proliferation of HUVECs by two different methods to identify its presence with high relevance in objective view. $PKC{\mu}$ will be investigated in further study. Methods: The study was conducted with the cultured HUVECs group(control) and the $0.75{\times}10^{-9}M$ DMH-treated group. After processing protein extraction in 0 and 24 hour, extracted protein was treated of quantitative test through BCA protein assay. In the western blot analysis, electrophoresis was performed in the order of gel preparation, sample preparation, and gel running. Electrotransfer to nitrocellulose membrane and reaction with antibody were done. Detection of $PKC{\alpha}$ was achieved through "Gel Image Analysis System". In the fluorescence immunocytochemical analysis, the grading of radiance of the intracellular $PKC{\alpha}$ particles was detected with confocal microscope after treating with primary and fluorescent secondary antibody in 0 and 24 hours. Results: The Western blot analysis showed increased $PKC{\alpha}$ expression from the specimen obtained in 24 hour of the DMH treatment group when compared to those in control group. Under confocal fluorescence microscope, the emitting radiance in the DMH treated group was brighter at 24 hours as well. Conclusion: We believe that $PKC{\alpha}$ plays a role in DMH-induced abnormal proliferation of the vascular endothelium, which may provide insights in understanding the vascular neoplasm.

• Journal of Korea Multimedia Society
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• v.21 no.5
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• pp.609-616
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• 2018

In this paper, we designed a multimodal bio-signal measurement system to observe changes in the brain nervous system and vascular system during sleep. Changes in the nervous system and the cerebral blood flow system in the brain during sleep induce a unique correlation between the changes in the nervous system and the blood flow system. Therefore, it is necessary to simultaneously observe changes in the brain nervous system and changes in the blood flow system to observe the sleep state. To measure the change of the nervous system, EEG, EOG and EMG signal used for the sleep stage analysis were designed. We designed a system for measuring cerebral blood flow changes using functional near-infrared spectroscopy. Among the various imaging methods to measure blood flow and metabolism, it is easy to measure simultaneously with EEG signal and it can be easily designed for miniaturization of equipment. The sleep stage was analyzed by the measured data, and the change of the cerebral blood flow was confirmed by the change of the sleep stage.

• Journal of radiological science and technology
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• v.17 no.2
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• pp.13-20
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• 1994

The paper is on the record of radiography the patients with SIEMENS ANGIOSTAR/POLYTRON S PLUS angiosystem with DSA system in the Chonbuk National University Hospital from October 1993 to June 1994. The results are as follow : 1. Among the total No, 836 case of utilization, angiography are 316(37.8 %), interventional radiography are 256(34.2 %), and dacryocystography and sialography are 168(20/1 %). Therefore non-angiogrlphy procedure are incerasing. 2. The aomunt of contrast material used are half as much as conventional angiovascular system. 3. The amount of film consumed are $10{\sim}15%$ of that of conventional angio vascular system and reduction of procedure time are $40{\sim}50%$. 4. Admitting that dignostic utility is in the utilization by examination region, there should be technological study which deal with fine vessel and motion artifact problem.

• Journal of Yeungnam Medical Science
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• v.34 no.1
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• pp.11-18
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• 2017

The pupillary size and movement are controlled dynamically by the autonomic nervous system; the parasympathetic system constricts the iris, while the sympathetic system dilates the iris. Under normal conditions, these constrictions and dilations occur identically in both eyes. Asymmetry in the pupillomotor neural input or output leads to impaired pupillary movement on one side and an unequal pupil size between both eyes. Anisocoria is one of the most common signs in neuro-ophthalmology, and the neurological disorders that frequently cause anisocoria include serious diseases, such as vascular dissection, fistula, and aneurysm. A careful history and examination can identify and localize pupillary disorders and provide a guide for appropriate evaluations.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.4 no.1
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• pp.26-31
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• 2018

Macrophages play a key role in atherosclerotic plaque formation, but their participation has been discerned largely via ex vivo analyses of atherosclerotic lesions. Therefore, we aimed to identify atherosclerosis on noninvasive in vivo imaging using reporter gene system. This study demonstrated that recruitment of macrophages could be detected in atherosclerotic plaques of Apolipoprotein E knockout (ApoE-/-) mice with a sodium iodide symporter (NIS) gene imaging system using $^{99m}Tc-SPECT$. This novel approach to tracking macrophages to atherosclerotic plaques in vivo could have applications in studies of arteriosclerotic vascular disease.

• Korean Journal of Pharmacognosy
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• v.19 no.3
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• pp.208-215
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• 1988

To investigate the experimental effects of Chunwangboshimdan which have been widely used for aterial or auricular flutter, neurosis, insomnia and disease caused by cardiac malfunction, we have done the effects on the central nervous and cardiovascular system. The result as follow; It inhibited the convulsion induced by caffeine, the writhing syndrome induced by acetic acid and the spontaneous movement. Furthermore it prolonged the sleeping time induced by thiopental-Na in mice. It also showed the negative inotropic action on the isolated heart of frog, the vasodilative action as to peripheral bloods of rabbit ear and the hypotensive action in the anesthetized rabbit.

• 한국전산유체공학회:학술대회논문집
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• 1999.11a
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• pp.152-157
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• 1999

For the simulation of the blood flow in coronary artery, the system modeling of coronary hemodynamics is combined with CFD technique. The blood flow in coronary artery interacts with the global coronary circulation. Especially in case of the coronary artery with stenosis, the interaction plays an important role in the hemodynamics of the circulation. In this study we present a combined numerical approach using both the CFD technique for flow simulation and the global system model of coronary circulation. We use a lumped parameter model for the global simulation of coronary circulation whereas the finite element method is employed to compute the viscous flow field in stenosed coronary artery, The time variation of the pressure drop due to stenosis is obtained from the proposed numerical method. Numerical results shows that the flow resistance and pressure drop due to stenosis has a relatively large value in systole.

• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.153-166
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• 1999

SAENGMAEGSAN(SMS) has been used in oriental medicine for many years as a therapeutic agent for cerebral disease. The effect of SMS on the vascular system is not known. The purpose of this study was to determine the effect of SMS on blood pressure, regio-nal cerebral blood flow(rCBF). 1. Blood pressure did not change by SMS in rats. 2. rCBF was increased by SMS in a dose-dependent manner. 3. Pretreatment with propranolol. methylene blue and indomethacin significantly inhibited SMS induced increase in rCBF. These results suggest that SMS causes a diverse response of blood pressure, regional cerebral blood flow (rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins. cyclic GMP and adrenergic ${\beta}$receptor.

• Korean Journal of Pharmacognosy
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• v.15 no.4
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• pp.188-193
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• 1984

It was previously indicated that 'Samulanshintang' had been examined for effects of the anticonvulsive, antipyretic, analgesic, sedative and antispasmodic actions. In order to investigate the effect on the cardiovascular system and anemic rabbits induced by phenlhydrazine HCl, pharmacological studies have been carried out with extract. The results of this investigation were summarized as follows: The relaxation of bronchial smooth muscle and antihistamine action were recognized. Antihypertensive and vasodilating actions due to vascular smooth muscle relaxation were noted in frogs and rabbits. 'Samulanshintang' lowered the rate of anemia induced by phenylhydrazine and rapidly recovered from the anemia state.

• Biomolecules & Therapeutics
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• v.28 no.1
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• pp.45-57
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• 2020

In the neurovascular unit, the neuronal and vascular systems communicate with each other. O₂ and nutrients, reaching endothelial cells (ECs) through the blood stream, spread into neighboring cells, such as neural stem cells, and neurons. The proper function of neural circuits in adults requires sufficient O₂ and glucose for their metabolic demands through angiogenesis. In a central nervous system (CNS) injury, such as glioma, Parkinson's disease, and Alzheimer's disease, damaged ECs can contribute to tissue hypoxia and to the consequent disruption of neuronal functions and accelerated neurodegeneration. This review discusses the current evidence regarding the contribution of oxygen deprivation to CNS injury, with an emphasis on hypoxia-inducible factor (HIF)-mediated pathways and Notch signaling. Additionally, it focuses on adult neurological functions and angiogenesis, as well as pathological conditions in the CNS. Furthermore, the functional interplay between HIFs and Notch is demonstrated in pathophysiological conditions.