• Journal of Conservation Science
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• v.38 no.2
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• pp.144-153
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• 2022

This study conducted scientific analysis and preservation treatment for the local 7 relics (depurator bottle, eagle patten button, rank insignia, mess tin, M1 Grand-gun cleaning tool kit, Carbine bullet, cartridge) of the fallen of Korean War excavated through the MND Agency for KIA Recovery & Identification that are possessed in the exhibition hall of the Ground Operations Command. FT-IR analysis results show that remainder of depurator bottle is assumed to play a role of water and food purifier during the war for its spectrum similar to Trisodium phosphate, and inscription carved on the cartridge that is hard to observe with naked eyes was also confirmed through 3D digital shooting method. This study also established directivity of preservation treatment of relics for stable treatment by carrying out a survey of conditions based on the microscope and visual observation. The result is expected to be used as basic data for establishment of future directivity of preservation treatment for a variety of war relics.

• Korean Journal of Ecology and Environment
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• v.55 no.3
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• pp.212-220
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• 2022

An estuary is a water ecosystem with a high abundance of the species diversity, due to a variety of complex physicochemical factors of the area where freshwater and ocean mixed. The identification of Corbicula species in the estuary environments is difficult because of various morphological characteristics. In this study, we provide taxonomic information on Corbicula species with taxonomic difficulties using morphological and genetic analysis. This study was conducted on clams from the Seomjin River-Gwangyang Bay, one of the major production area of marsh clam in Korea. As a result, we characterized Cytocrome C Oxidase subunit I (COI) sequences of the Corbicula. The 636 bp nucleotide sequences of COI have 98% homology among Corbicula species collected from 2 sites of Seomjin River-Gwangyang Bay. The phylogenetic analysis with 17 species of Corbicula indicated that most of the species collected from Seomjin River-Gwangyang Bay were brackish water clam (Corbicula japonica), and only one Asian clam (Corbicula fluminea). The evolutionary distance between C. japonica and C. fluminea was less than 0.003. Therefore, it was confirmed that C. japonica is phylogenetically closely related to C. fluminea. In 9 species of Cyrenidae, phylogenetic tree was classified into three lineages. These results will be used as an important data for an identification of clam species by providing genetic information for Corbicula species with a morphological diversity.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.34 no.1
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• pp.115-127
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• 2024

The recent increase in digital audio media has greatly expanded the size and diversity of sound data, which has increased the importance of sound data analysis in the digital forensics process. However, the lack of standardized procedures and guidelines for sound data analysis has caused problems with the consistency and reliability of analysis results. The digital environment includes a wide variety of audio formats and recording conditions, but current audio forensic methodologies do not adequately reflect this diversity. Therefore, this study identifies Life-Cycle-based sound data elemental technologies and provides overall guidelines for sound data analysis so that effective analysis can be performed in all situations. Furthermore, the identified elemental technologies were analyzed for use in the development of digital forensic techniques for sound data. To demonstrate the effectiveness of the life-cycle-based sound data elemental technology identification system presented in this study, a case study on the process of developing an emergency retrieval technology based on sound data is presented. Through this case study, we confirmed that the elemental technologies identified based on the Life-Cycle in the process of developing digital forensic technology for sound data ensure the quality and consistency of data analysis and enable efficient sound data analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.2
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• pp.283-294
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• 2007

Conventional culture-based methods of enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) are being rapidly replaced by nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation. The foundation of these techniques is 16S/18S rDNA sequence analysis which has provided a phylogenetically based classification scheme for enumeration and identification of microbial community members. While these analyses are very informative for determining the composition of the microbial community and monitoring changes in population size, they can only infer function based on these observations. The next step in functional analysis of the ecosystem is to measure how specific and, or, predominant members of the ecosystem are operating and interacting with other groups. It is also apparent that techniques which optimise the analysis of complex microbial communities rather than the detection of single organisms will need to address the issues of high throughput analysis using many primers/probes in a single sample. Nearly all the molecular ecological techniques are dependant upon the efficient extraction of high quality DNA/RNA representing the diversity of ruminal microbial communities. Recent reviews and technical manuals written on the subject of molecular microbial ecology of animals provide a broad perspective of the variety of techniques available and their potential application in the field of animal science which is beyond the scope of this treatise. This paper will focus on nucleic acid based molecular methods which have recently been developed for studying major functional groups (cellulolytic bacteria, protozoa, fungi and methanogens) of microorganisms that are important in nutritional studies, as well as, novel methods for studying microbial diversity and function from a genomics perspective.

• Toxicological Research
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• v.18 no.1
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• pp.59-64
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• 2002

Substantial evidences have been accumulated about the hormone-like effects of exogenous substances such as pesticides and industrial chemicals during past years. The effects of these substances on the endocrine system are believed to be either enhancing or reducing of various endocrine action. It is necessary to identify putative causal agents by the batter system and to assess their ability to disrupt the endocrine system. A variety of in vitro and In vivo approaches have been used to determine the androgenic effects of environmental chemicals. To establish the method for assessment of the putative endocrine disruptors with androgenic activity, we carried out the cell proliferation assay by MTS method after treatment with the various concentration of testosterone in LNCaP cells (human prostatic cancer cell line) and also observed the expression of androgen-related genes by quantitative RT-PCR. In the cell proliferation assay, the results showed that the grouth of LNCaP cells increased within level of at least 10pM testosterone. We measured by quantitative RT-PCR method on the effects of testosterone on mRNA expression of androgen receptor (AR), prostate-specific antigen (PSA), bone morphogenetic protein (BMP) and BMP receptor (BMPR) In LNCaP cells. The results demonstrated that mRNA expression of PSA and BMPR-IB was observed differently within level of at least 0.01 pM testosterone compared with non-treated control. These observations suggest that the detection of PSA and BMPR-IB mRNA by the quantitative RT-PCR in LNCaP cells is very sensitive method to identify the endocrine disruptors to have the androgenic effects.

• Journal of Digital Convergence
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• v.11 no.2
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• pp.299-307
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• 2013

The aim of this paper is the improvement of identification performance and the reduction of computational quantities in the face recognition system based on SIFT-Grid. Firstly, we propose a composition method of integrated template by removing similar SIFT keypoints and blending different keypoints in variety training images of one face class. The integrated template is made up of computation of similarity matrix and threshold-based histogram from keypoints in a same sub-region which divided by applying SIFT-Grid of training images. Secondly, we propose a computation method of similarity for identify of test image from composed integrated templates efficiently. The computation of similarity is performed that a test image to compare one-on-one with the integrated template of each face class. Then, a similarity score and a threshold-voting score calculates according to each sub-region. In the experimental results of face recognition tasks, the proposed methods is founded to be more accurate than both two other methods based on SIFT-Grid, also the computational quantities are reduce.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.4D
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• pp.585-594
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• 2011

A variety of PPP (Public-Private Partnerships) schemes including BOT, TOT (Transfer-Operate-Transfer), and divestiture has been employed in the Chinese water sector. Although the two latter emerging models have been implemented in their full applications lately, there has been no follow-up for identification of opportunities and challenges inherent in those schemes in relation to the market participation of foreign investor. This study undertakes in-depth analysis on the TOT and divestiture projects in the SWOT (Strengths, Weaknesses, Opportunities, and Threats) matrix and measures the level of agreement on each SWOT through a survey among the Chinese water market experts. The findings can be used to support foreign investors in managing the weaknesses and threats that, unless properly managed, can be evolved into potential project risks, while fully benefiting from the strengths and opportunities of the two schemes.

• Journal of Environmental Health Sciences
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• v.38 no.2
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• pp.151-158
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• 2012

Objectives: Bioaerosols released by treating organic-waste resources cause a variety of environmental and hygiene problems. The objective of this study was to investigate the distribution characteristics of the airborne bacteria emitted from a pig manure composting plant, a principal site for organic-waste resource facilities. Methods: Three types of pig manure composting plant were selected based on fermentation mode: screw type, rotary type and natural-dry type. Each site was visited and investigated on a monthly basis between September 2009 and August 2010. A total of 36 air samplings were obtained from the pig manure composting plants. The air sampling equipment was a six-stage cascade impactor. Quantification and qualification of airborne bacteria in the air samples was performed by agar culture method and identification technique, respectively. Results: The mean concentrations of airborne bacteria in pig manure composting plant were 7,032 (${\pm}1,496$) CFU $m^{-3}$ for screw type, 3,309 (${\pm}1,320$) CFU $m^{-3}$ for rotary type, and 5,580 (${\pm}1,106$) CFU $m^{-3}$ for natural dry type. The screw type pig manure composting plant showed the highest concentration of airborne bacteria, followed by the natural dry type and the rotary type. The ratio of respirable to total airborne bacteria was approximately 40-60%. The predominant genera of airborne bacteria identified were Micrococcus spp., Staphylococcus spp. and Escherichia spp. Conclusion: Monthly levels of airborne bacteria were highest in August and lowest in November regardless of fermentation mode. There was no significant correlation relationship between airborne bacteria and environmental factors such as temperature, relative humidity and particulate matters in pig manure composting plants.

• Journal of the Korean Society for information Management
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• v.29 no.4
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• pp.43-60
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• 2012

Identifying citing sentences from article full-text is a prerequisite for creating a variety of future academic information services such as citation-based automatic summarization, automatic generation of review articles, sentiment analysis of citing statements, information retrieval based on citation contexts, etc. However, finding citing sentences is not easy due to the existence of implicit citing sentences which do not have explicit citation markers. While several methods have been proposed to attack this problem for English, it is difficult to find such automatic methods for Korean academic literature. This article presents a rule-based approach to identifying Korean citing sentences. Experiments show that the proposed method could find 30% of implicit citing sentences in our test data in nearly 70% precision.

• Journal of Forest and Environmental Science
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• v.25 no.2
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• pp.93-100
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• 2009

In Malaysia, Labisia pumila Benth & Hook f, popularly known as 'Kacip Fatimah' has been used traditionally to treat various elements of the woman's health in Malay community. The objective of this study was to develop randomly amplified polymorphic DNA (RAPD) based DNA markers for the identification of L. pumila and to distinguish its three varieties from each other. Total DNA from nine accessions of L. pumila was extracted by CTAB method and polymerase chain reactions (PCR) were carried out to amplify the segments of DNA using different primers to develop DNA barcode using RAPD technique. To find out variety-specific DNA marker/s, twenty different 10-mer primer sequences with annealing temperature from 36-$40^{\circ}C$ were evaluated in triplicate. Out of 20 random primers, two primers (OPA-1 and OPA-2/A10) were selected which produced reliable RAPD band patterns. To have DNA based handle, two RAPD amplification products were cloned and sequenced to determine the identity of the DNA. RAPD analysis using two random primers generated 72 discrete bands ranging in size 200 bp-3,000 bp. Fifty nine of these were polymorphic loci (82%) and thirteen were non-polymorphic loci (18%). A total of 32 bands polymorphic loci (72%) were amplified with primer OPA-1 and analyzed by cluster analysis and UPGMA (Unweighted Pair Group Method with Arithmetic) to present a dendogram depicting the degree of genetic relationship among nine accessions of L. pumila. Our results shows the reasonable genetic diversity among the L. pumila varieties and within varieties; and two RAPD marker sequences obtained could be used to identify L. pumila at species level.