• BMB Reports
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• 제39권1호
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• pp.55-60
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• 2006

We describe a phage display strategy, based on the differential resistance of proteins to denaturant-induced unfolding, that can be used to select protein variants with improved conformational stability. To test the efficiency of this strategy, wild-type and two stable variants of ${\alpha}_1$-antitrypsin (${\alpha}_1AT$) were fused to the gene III protein of M13 phage. These phages were incubated in unfolding solution containing denaturant (urea or guanidinium chloride), and then subjected to an unfavorable refolding procedure (dialysis at $37^{\circ}C$). Once the ${\alpha}_1AT$ moiety of the fusion protein had unfolded in the unfolding solution, in which the denaturant concentration was higher than the unfolding transition midpoint ($C_m$) of the ${\alpha}_1AT$ variant, around 20% of the phage retained binding affinity to anti-${\alpha}_1AT$ antibody due to a low refolding efficiency. Moreover, this affinity reduced to less than 5% when 10 mg/mL skimmed milk (a misfolding-promoting additive) was included during the unfolding/refolding procedure. In contrast, most binding affinity (>95%) remained if the ${\alpha}_1AT$ variant was stable enough to resist unfolding. Because this selection procedure does not affect the infectivity of M13, the method is expected to be generally applicable to the high-throughput screening of stable protein variants, when activity-based screening is not possible.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1683-1686
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• 2012

In the present case control study mRNA expression of the GSTP1 gene, encoding a phase II enzyme that detoxifies via glutathione conjugation, was investigated using semiquantitative PCR followed by SSCP for 49 confirmed head and neck (HN) cancer and 49 control samples. It was found that GSTP1 was upregulated in significantly higher number of cancers (OR 4.2, 95% CI 1.2-15.3). Grade wise correlation was also observed with more up regulation in patients with more advanced grades of HN carcinomas. We also found that 5 patients showed variation in mRNA with a larger product size than expected. Sequencing revealed insertion of an intronic segment between the $6^{th}$ and $7^{th}$ exon of the GSTP1 gene. Germline screening was performed showing mobility shifts which suggested mutation at the DNA level resulting in intronic portion retention. This study is of prime importance for drug design and treatment selection to overcome increased resistance of HN cancers to drugs due to alteration in the GSTP1 gene.

• 한국컴퓨터정보학회논문지
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• 제13권1호
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• pp.125-133
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• 2008

모션 블러란 카메라나 촬영대상의 움직임에 의해서 화소가 혼합되어 이미지의 경계가 흐려지는 현상을 말한다. 촬영 순간 카메라와 촬영 대상간의 상대적 움직임에 의해 촬영된 이미지에는 움직인 방향으로 모션 블러가 발생할 수 있다. 촬영 대상들이 서로 다른 속도로 이동하면 지역적으로 다양한 크기의 모션 블러가 발생할 수 있다. 이러한 지역적으로 다양한 모션 블러가 발생한 이미지를 복원하기 위해서는 지역적으로 다양한 블러의 크기를 추출해야만 한다. 본 논문에서는 지역적으로 다양한 모션 블러가 발생된 이미지로부터 RATS(Robust Automatic Threshold Selection)를 이용하여 지역적으로 다양한 블러의 크기를 각 지역별로 추출하는 새로운 방법을 제안한다. 또한 이전 방법과의 비교 실험을 통해 성능을 검증한다.

• 한국컴퓨터정보학회논문지
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• 제15권4호
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• pp.11-18
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• 2010

Fuzzy c-means(FCM)는 퍼지 집합을 응용한 간단하지만 효율적인 클러스터링 방법 중 하나이다. FCM은 여러 응용 분야에서 성공적으로 활용되어 왔지만, 초기화와 잡음에 민감하고 볼록한 형태의 클러스터들만 다룰 수 있는 문제점이 있다. 이 논문에서는 이러한 FCM의 문제점을 해결하기 위해 전역 클러스터링(global clustering) 기법과 커널 클러스터링(kernel clustering) 기법을 결합하여 새로운 비선형 클러스터링 기법인 커널 전역 FCM(kernel global fuzzy c-means, KG-FCM)을 제안한다. 전역 클러스터링은 클러스터링의 초기화를 위한 방법 중 하나로, 순차적으로 클러스터를 하나씩 추가함으로써 초기화에 민감한 FCM의 한계를 극복할 수 있도록 해준다. FCM의 잡음 민감성과 볼록한 클러스터들만 다룰 수 있는 한계를 극복하기 위한 방법은 여러 가지가 있으며 커널 클러스터링이 그 중 하나이다. 커널 클러스터링은 사용하는 커널을 바꿈으로써 쉽게 확장이 가능하므로 이 논문에서는 커널 클러스터링을 사용하였다. 두 방법을 결합함으로써 제안한 방법은 위에서 언급한 문제점들을 해결할 수 있으며, 이는 가상 및 실제 데이터를 이용한 실험 결과를 통해 확인할 수 있다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권3호
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• pp.921-940
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• 2015

In this paper, we investigate the multi-resource allocation problem, a unique feature of which is that the multiple resources can compensate each other while achieving the desired system performance. In particular, power and time allocations are jointly optimized with the target of energy efficiency under the resource-limited constraints. Different from previous studies on the power-time tradeoff, we consider a multi-server case where the concurrent serving users are quantitatively restricted. Therefore user selection is investigated accompanying the resource allocation, making the power-time tradeoff occur not only between the users in the same server but also in different servers. The complex multivariate optimization problem can be modeled as a variant of 2-Dimension Bin Packing Problem (V2D-BPP), which is a joint non-linear and integer programming problem. Though we use state decomposition model to transform it into a convex optimization problem, the variables are still coupled. Therefore, we propose an Iterative Dual Optimization (IDO) algorithm to obtain its optimal solution. Simulations show that the joint multi-resource allocation algorithm outperforms two existing non-joint algorithms from the perspective of energy efficiency.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.227-232
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• 2003

Radiation technique has been used to develope mutant rice. Suwon 345 rice seeds were irradiated with 250 Gy gamma ray. Morphological characteristics of the variants in M$_{8}$ generation were observed and random amplified polymorphic DNA(RAPD) analysis was carried out. Plant height, panicle length, 1,000 grain weight and lodging were very different in mutants compared with donor cultivar. RAPD analysis showed that polymorphic bands were presented in several primers of the mutants. In comparison with original variety, variants were classified into four group through UPGMA analysis. A group has mutation trait in panicle length, B group in plant height and C group in 1,000 grain weight. Among mutants, no. 46 and 147 was ranked as salt tolerance and the malonaldehyde content of these mutants was more increased than that of original variety. Valuable mutants obtained will be useful for developing new cultivars and for studing gene function in molecular level.l.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.220-220
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• 2022

Disproportionating Enzyme 1 (DPE1) is an a-1,4-D-glucanotransferase that cleavages the a-1,4-glucosidic bonds and transfers glucosyl groups. In rice endosperm, it participates in starch synthesis by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Here, we investigated the haplotype variations and evolutionary indices (e.g., genetic diversity and population structure) for the DPE1 gene in 374 rice accessions representing seven subgroups (wild, indica, temperate japonica, tropical japonica, aus, aromatic, and admixture). Variant calling analysis of DPE1 coding regions leads to the identification of six functional haplotypes representing/occupying 8 nonsynonymous SNPs. Nucleotide diversity analysis revealed the highest pi-value in wild group (0.0556) compared to other cultivated groups, of which temperate japonica showed the most reduction of genetic diversity value (0.003). A significant positive Tajima's D value (1.6330) of admixture highlights sudden population contraction under balancing selection, while temperate japonica with the lowest Tajima's D value (-1.3523) showed a selection signature of DPE1 domestication which might be the cause of excess of rare alleles. Moreover, these two subpopulations exhibits a greater differentiation (F_ST=0.0148), indicating a higher genetic diversity. Our findings on functional DPE1 haplotypes will be useful in future breeding programs, and the evolutionary indices can also be applicable in functional studies of the DPE1 gene.

• ETRI Journal
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• 제35권1호
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• pp.27-34
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• 2013

This paper proposes a network-adaptive mechanism for HTTP-based video streaming over wireless/mobile networks. To provide adaptive video streaming over wireless/mobile networks, the proposed mechanism consists of a throughput estimation scheme in the time-variant wireless network environment and a video rate selection algorithm used to increase the streaming quality. The adaptive video streaming system with proposed modules is implemented using an open source multimedia framework and is validated over emulated wireless/mobile networks. The emulator helps to model and emulate network conditions based on data collected from actual experiments. The experiment results show that the proposed mechanism provides higher video quality than the existing system provides and a rate of video streaming almost void of freezing.

• Journal of electromagnetic engineering and science
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• 제4권4호
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• pp.190-196
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• 2004

An alternative solution to the problem of obtaining acceptable performances on a fading channel is the diversity technique, which is widely used to combat the fading effects of time-variant channels. The symbol error probability of M-ary DPSK(MDPSK), PSK(MPSK) and QAM(MQAM) systems using 2 branches from the branch with the largest signal-to-noise ratio(SNR) at the output of L-branch selection combining(SC), i.e., SC2 in frequency- nonselective slow Nakagami fading channels with an additive white Gaussian noise(AWGN) is derived theoretically. These performance evaluations allow designers to determine M-ary modulation methods for Nakagami fading channels.

• Journal of electromagnetic engineering and science
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• 제7권1호
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• pp.35-41
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• 2007

An alternative solution to the problem of obtaining acceptable performances on a fading channel is the diversity technique, which is widely used to combat the fading effects of time-variant channels. The symbol error probability of M-ary DPSK (MDPSK), PSK (MPSK) and QAM (MQAM) systems using 2 branches from the branch with the largest signal-to-noise ratio(SNR) at the output of L-branch selection combining(SC), i.e., SC2 in frequency-nonselective slow Nakagami fading channels with an additive white Gaussian noise(AWGN) is derived theoretically. These performance evaluations allow designers to determine M-ary modulation methods against Nakagami fading channels.