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http://dx.doi.org/10.5010/JPB.2003.30.3.227

Selection and Agronomics Characterization of Radiation-Induced Variants in Rice  

Lee, In-Sok (Korea Atomic Energy Research Institute)
Kim, Dong-Sup (Korea Atomic Energy Research Institute)
Choi, Su-Ryun (Department of Horticulture, Chungnam National University)
Song, Hi-Sup (Korea Atomic Energy Research Institute)
Lee, Sang-Jae (Korea Atomic Energy Research Institute)
Lim, Yong-Pyo (Department of Horticulture, Chungnam National University)
Lee, Young-Il (Korea Atomic Energy Research Institute)
Publication Information
Journal of Plant Biotechnology / v.30, no.3, 2003 , pp. 227-232 More about this Journal
Abstract
Radiation technique has been used to develope mutant rice. Suwon 345 rice seeds were irradiated with 250 Gy gamma ray. Morphological characteristics of the variants in M$_{8}$ generation were observed and random amplified polymorphic DNA(RAPD) analysis was carried out. Plant height, panicle length, 1,000 grain weight and lodging were very different in mutants compared with donor cultivar. RAPD analysis showed that polymorphic bands were presented in several primers of the mutants. In comparison with original variety, variants were classified into four group through UPGMA analysis. A group has mutation trait in panicle length, B group in plant height and C group in 1,000 grain weight. Among mutants, no. 46 and 147 was ranked as salt tolerance and the malonaldehyde content of these mutants was more increased than that of original variety. Valuable mutants obtained will be useful for developing new cultivars and for studing gene function in molecular level.l.
Keywords
Radiation; variant selection; rice; variated characters;
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