• 생약학회지
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• 제39권2호
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• pp.91-94
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• 2008

Column chromatographic separation of the MeOH extract from the aerial parts of Paris verticillata led to the isolation of three phenolics, two terpene glucosides and two pyrrolidine alkaloids. Their structures were characterized to be methyl caffeate (1), 5-hydroxy pyrrolidin-2-one (2), vanillic acid (3), benzyl alcohol glucopyranoside (4), (6S, 9R)-roseoside (5), staphylionoside H (6) and 5-methoxy pyrrolidin-2-one (7) by spectroscopic means. The isolated compounds (1-7) were for the first time reported from this source. The isolated compounds were tested for their cytotoxicity against four human tumor cell lines by SRB method in vitro.

• 한국식품영양과학회지
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• 제27권5호
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• pp.1015-1018
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• 1998

Capsaicin(8-methyl-N-vanillyl-6-nonenamide: CAP) known well as a major compound of not taste in hot pepper, was investigated for the inhibition effect on in vitro nitrosation. CAP(100$\mu$mol) inhibited the formation of N-nitrosoproline(NPRO) and N-nitrosothioproline(NTPRO) by 56% and 26%, respectively. Vanillyl alcohol inhibited the nitrosation of proline by a concentration-dependent manner, and vanillic acid and vanillin were less effective in blocking the nitrosation of proline compared to CAP and anillyl alcohol. The inhibitory effect of NPRO formation by CAP was evaluated to similar with alpha-tocopherol, and vanillyl alcohol was more effective than alpha-tocopherol in blocking the nitrosation of proline. Our results suggested that CAP and its metabolites such as vanillyl alcohol could inhibit endogenous nitrosation in hydrophobic biological environment.

• Journal of the Korean Wood Science and Technology
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• 제31권3호
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• pp.11-16
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• 2003

On nitrobenzene oxidation of aspen, spruce, and knauf wood meals gave rise to vanilline, syrigaldehyde, p-hydroxybenzoaldehyde, vanillic acid, and other minor oxidation products. The phenolic aldehydes (p-hydroxybenzaldehyde, vanilline, and syringaldehyde) are derived from oxidative degradation of the corresponding 4-hydroxyphenylpropane units and their ethers. The lignin content of knauf wood meals was different as the concentration of NaOH solution and cooking temperature. The lignin contents of aspen, spruce, and knauf wood meals were decreased as laccase treatment. The laccase caused C-oxidation, demethylation, cleavage in phenolic groups and C-C cleavage in syrigyl structures.

• 생명과학회지
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• 제25권7호
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• pp.786-794
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• 2015

본 연구는 황금찰수수의 에탄올 추출물을 이용하여 항산화 활성 및 뉴라니미데이즈 억제 효과를 조사하였다. 헥산, 에틸아세테이트, 메탄올과 70% 에탄올 조추출물에서 항산화력의 IC₅₀값을 비교한 결과 70% 에탄올 추출물이 DPPH 라디칼 소거능, ABTS 라디칼 소거능에서 각각 83.2±2.7, 85.6±2.4 μg/ml으로 가장 높았고, 뉴라니미데이즈 억제활성 IC₅₀값을 비교한 결과 메탄올 추출물에서 1.8±0.1 μg/ml로 나타났다. 또한 황금찰수수 추출물에서 컬럼크로마토그래피와 UPLC-PDA-MS/MS 분광기 분석을 통해, Gallic acid (1), Protocatecuic acid (2), p-Hydroxy benzoic acid (3), Vanillic acid (4), Caffeic acid (5), Ferulic acid (6), Luteolinidin (7), Apigeninidin (8), Luteolin (9), 총 9종의 폴리페놀 화합물을 확인하였다. 또한 각각의 화합물에 대한 항산화력의 IC₅₀값을 비교한 결과 Luteolinidin이 DPPH 라디칼 소거능, ABTS 라디칼 소거능에서 각각 10.9±0.5, 8.6±3.0 μM로 가장 우수 하였고, 뉴라니미데이즈 억제활성은 Luteolin이 12.9±3.8의 IC₅₀값과 비경쟁적 저해모델을 보였다. 결과적으로 황금찰수수는 높은 항산화 효과와 뉴라미니데이즈 억제활성을 보여 식품, 사료 등의 새로운 기능성소재로 다양하게 활용될 수 있음을 시사한다.

• 한국자원식물학회지
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• 제26권3호
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• pp.355-361
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• 2013

The present study attempts to evaluate antioxidant activities of extracts from Abeliophyllum distichum. Nakai flower. The samples were collected in Jangyyeon-myeon, Goesan-gun, Korea and extracted with either hot-water or ethyl acetate (EtOAC). In DPPH, hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity of EtOAC extracts were 93.41%, 98.43%, and 7.38%, while those of hot-water extracts were 86.93%, 41.33% and 47.68% at 200 ${\mu}g/ml$, respectively. In ${\varphi}X$-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 82% and 17% at 200 ${\mu}g/ml$, respectively. Both extracts showed the protective effect of DNA migration by oxidative stress in intracellular DNA migration assay. Both extracts had no cytotoxity in NIH3T3 cells. Several polyphenolic compounds were identified such as 2-methoxy-benzoic acid, vanillic acid, phytol and pulegone by GC/MS. These results indicated that extracts of Abeliophyllum distichum Nakai flower showed antioxidant activities and protective activities against oxidative DNA damage and showed the possibility to be used as an effective natural antioxidants.

• Natural Product Sciences
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• 제15권1호
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• pp.44-49
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• 2009

Seven monoterpenes, three sesquiterpenes, three phenolics and one flavonoid were isolated from the MeOH extract of Amomum xanthioides. Their structures were determined by spectroscopic methods to be caryophyllene oxide (1), bornyl acetate (2), nerolidol (3), spathulenol (4), (-)-borneol (5), (+)-5-endohydroxycamphor (6), vanillic acid (7), protocatechuic acid methyl ester (8), betulabuside A (9), (1R,4S,6R)-6-hydroxyfenchan-2-one-6-O-$\beta$-D-glucopyranoside (10), (1S,4R,6S)-6-hydroxybornan-2-one-6-O-$\beta$-D-glucopyranoside (11), (1R,2S,4S,5R)-angelicoidenol 2-O-$\beta$-D-glucopyranoside (12), 1-O-vanilloyl-$\beta$-D-glucopyranoside (13), and quercetin-3-rhamnopyranoside (14). Compounds 6-14 were isolated for the first time from this plant source. Compounds 3 and 4 exhibited moderate cytotoxicity against four human cancer cell lines in vitro using a SRB bioassay.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.475-482
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• 2014

The metabolic pathway of eugenol degradation by thermophilic Geobacillus sp. AY 946034 strain was analyzed based on the lack of data about eugenol degradation by thermophiles. TLC, GC-MS, and biotransformation with resting cells showed that eugenol was oxidized through coniferyl alcohol, and ferulic and vanillic acids to protocatechuic acid before the aromatic ring was cleaved. The cell-free extract of Geobacillus sp. AY 946034 strain grown on eugenol showed a high activity of eugenol hydroxylase, feruloyl-CoA synthetase, vanillate-O-demethylase, and protocatechuate 3,4-dioxygenase. The key enzyme, protocatechuate 3,4-dioxygenase, which plays a crucial role in the degradation of various aromatic compounds, was purified 135-fold to homogeneity with a 34% overall recovery from Geobacillus sp. AY 946034. The relative molecular mass of the native enzyme was about $450{\pm}10$ kDa and was composed of the non-identical subunits. The pH and temperature optima for enzyme activity were 8 and $60^{\circ}C$, respectively. The half-life of protocatechuate 3,4-dioxygenase at the optimum temperature was 50 min.

• 산업식품공학
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• 제22권4호
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• pp.295-303
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• 2018

Polyphenol profiles, physicochemical properties, antioxidant activities, and inhibitory effect of adipocyte differentiation of Houttuynia cordata fermented with Lactobacillus brevis B84 were evaluated. Six polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and the results were compared with total phenolic content by a spectrophotometric method. The total amount of the identified polyphenols was lower than that determined by the spectrophotometric method. However, the fermentation process influenced polyphenol composition such as content of vanillic acid and caffeic acid. The phytochemical profiles were evaluated by high-performance liquid chromatography with UV and electrospray ionization mass spectrometry detection ($HPLC-DAD-ESI-MS^n$). Total sugar and reducing sugar contents decreased after fermentation. Antioxidant activities such as DPPH, ABTS, and superoxide anion radical scavenging and reducing power were evaluated to compare the beneficial effect after fermentation. Fermented H. cordata increased the lipolytic effect in 3T3-L1 adipocytes. Overall, the results indicate that the fermentation of H. cordata with L. brevis B84 produces changes of phenolic compounds, antioxidant activity, and lipolytic effect.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.338-344
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• 2023

Curcuma longa is a plant belonging to the genus Curcuma and is distributed across various Asian regions. This plant is widely known for its rhizomes, which possess a variety of pharmacological properties. However, although the leaves and stems of this plant also contain several health-promoting secondary metabolites, very few studies have characterized these compounds. Therefore, our study sought to quantify the secondary metabolites from the leaves and stems of Curcuma longa L. (LSCL) using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC). Our LC-ESI-MS analyses detected twenty-one phenolic compounds in the LSCL, among which fifteen compounds were detected via HPLC analysis. Four compounds, namely vanillic acid (0.129 mg/g), p-coumaric acid (0.431 mg/g), 4-methylcatechol (0.199 mg/g), and afzelin (0.074 mg/g) were then quantified. These findings suggest that LSCL is rich in secondary metabolites and holds potential as a valuable resource for the development of functional and nutritional supplements in the future.

• Journal of Ginseng Research
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• 제16권3호
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• pp.228-234
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• 1992

There has been general tendency to explain the traditional ginseng efficacy through the pharmacological and biochemical activities of ginsenosides. However, when we analyze the pharmacological and biological data on ginseng reported yet, we can easily arrive at the conclsion that most of the data on pharmacological and biological activities must have been obtained using impure ginsenoside samples, which should contain some non-saponin constituents as impurities. Based on the above back-ground, the non-saponin constituents of ginseng were studied in our laboratory. Phenolic substances including Maltol, Vanillic Acid, Salicylic Acid, Ferrulic Acid and Caffeic acid and impure ginsenoside samples were found to show strong antioxidant and anti-fatigue activities, while pure ginsenosides were devoid of the activities. Maltol, one of antioxidant components In Korean red ginseng drew a special interest due to its very low pro-oxidant activity. The antioxidant activity of ginseng may be considered as scientific basis for the antiageing activity which was described in traditional medicinal material book as "long-term medication of ginseng will improve bio-efficiency and extend life-span" The lignin components, another non-saponin consitutents, isolated from ginseng extract In our laboratory may eplain the hepato-protective activity of ginseng which has been repeatedly rtaimed as one of the efficacies of ginsenosides. The P-carboline alkaloids isolated in our laboratory as one of the non-saponin constituents of ginseng may play some pharmacological activities which should also be investigated. Present paper will include chemistry and biochemical aspects of the non-saponin constituents of ginseng with special interests for the explanation of traditional ginseng efficacy on modern scientific basis.fic basis.