• Journal of Plant Biotechnology
• /
• 제37권3호
• /
• pp.250-261
• /
• 2010

The expression of vaccine antigens in transgenic plants has the potential to provide a convenient, stable, safe approach for oral vaccination alternative to traditional parenteral vaccines. Over the past two decades, many different vaccine antigens expressed via the plant nuclear genome have elicited appropriate immunoglobulin responses and have conferred protection upon oral delivery. Up to date, efforts to produce antigen proteins in plants have focused on potato, tobacco, tomato, banana, and seed (maize, rice, soybean, etc). The choice of promoters affects transgene transcription, resulting in changes not only in concentration, but also in the stage tissue and cell specificity of its expression. Inclusion of mucosal adjuvants during immunization with the vaccine antigen has been an important step towards the success of plant-derived vaccines. In animal and Phase I clinical trials several plant-derived vaccine antigens have been found to be safe and induce sufficiently high immune response. Future areas of research should further characterize the induction of the mucosal immune response and appropriate dosage for delivery system of animal and human vaccines. This article reviews the current status of development in the area of the use of plant for the development of oral vaccines.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
• /
• pp.295.2-295.2
• /
• 2003

Lipid nanodispersion (LN) composed of biocompatible lipids and surfactants is an alternative parenteral drug delivery system especially for lipophilic drugs. It has been studied for versatile applications such as oral, parenteral, topical, ocular, vaccine, and peptide drug delivery. The purpose of this study was to produce a novel LN system for intravenous injection using the high pressure homogenization. (omitted)

• KSBB Journal
• /
• 제19권5호
• /
• pp.341-347
• /
• 2004

Several gene delivery therapies are being developed for treatment of serum protein deficiency. EPO is one of the most promising therapeutic agent for this treatment which is currently being investigated in depth. This study has the ultimate purpose of improving the gene delivery system for an increase of red blood cell production. A plasmid DNA was constructed smaller than other plasmids for an increase in penetration into animal cells, and two genes were cloned into each vector as a co-delivery system to express erythropoietin, and interluekin-3 or thrombopoietin, which can act on erythroid cell, thus activating hematopoiesis synergically. This co-delivery system has an advantage of decreasing the labour required for industrial production of DNA vaccine. A new plasmid vector, pVAC, in size 2.9 kb, was constructed with the essential parts from PUC 19 and pSectagB, which is much smaller than other plasmid vector and is the size of 2.9 kb. Co-delivery system was constituted by cloning human erythropoietin with each of human interluekin-3 gene or human thrombopoietin gene into both pVAC and pSectagB. As a result, the transfection efficiency of pVAC was higer than that of pSectagB in vitro, and hematocrit level of the mice injected with pVAC is higher than that of other mice. And co-delivery system, made of several plasmid DNAs, was expressed in vitro.

• 적정기술학회지
• /
• 제7권1호
• /
• pp.26-32
• /
• 2021

콜드체인의 마지막 단계인 백신전달 과정에서 대부분 얼음을 넣은 단열컨테이너로 온도를 조절하며, 백신의 온도 조절이 적절하지 않아 낭비되는 백신의 양이 상당하다. 예방접종을 관리하고 운영하는데 온도 데이터가 중요하며, 본 연구에서는 실시간 백신전달 모니터링 시스템을 개발하였다. 휴대용 백신 냉장고의 온도, 위치 및 전력 소비 데이터를 수집하기 위해 문자(Short Message Service) 통신 방법을 사용하였고, 한국과 탄자니아(아루샤 및 킬리만자로 지역)에서 시스템을 테스트하였다. 개발된 백신 냉장고는 문자통신을 통해 데이터 손실없이 실시간으로 백신상태를 모니터링을 할 수 있었다. 백신의 상태 및 데이터는 클라우드 서버를 통해 웹 기반 유저 인터페이스에서 확인할 수 있었다.

• 대한의생명과학회지
• /
• 제12권3호
• /
• pp.177-183
• /
• 2006

To demonstrate the effect of formulation conditions and evaluations of structural integrity from ovalbumin containing poly lactide glycolide copolymer (PLGA) microspheres for Vaccine delivery, OVA microspheres were prepared by a W/O/W multiple emulsion solvent extraction technique. Dichloromethan (DCM) and Ethyl acetate (EA) were applied as an organic phase and poly vinyl alcohol (PVA) as a secondary emulsion stabilizer. Microspheres were characterized for particle size, morphology (optical microscopy and Scanning Electron Microscope (SEM)). Protein denaturation was evaluated by size exclusion chromatography (SEC), SDS-PAGE and isoelectric focusing (IEF). Residual organic solvent was estimated by gas chromatography (GC) and differential scanning calorimetry (DSC). Optical photomicrograph and SEM revealed that micro spheres were typically spherical but various morphologies were observed. Mean particle size $(d_{vs})$ of microspheres were in the range of $3{\sim}50{\mu}m$. Also, The protein stability was not affected by the fonnulation process and residual organic solvent was beyond the detection below 0.1ppm. These results demonstrated that micro spheres might be a good candidate for the parenteral vaccine delivery system.

• 대한수의사회지
• /
• 제30권12호
• /
• pp.710-728
• /
• 1994

이 논문은 국내에서의 새로운 vaccine의 연구 및 개발에 참고가 되었으면 하는 뜻에서, D.T.O'Hagan저 'Novel Delivery Systems for Oral vaccines'(CRC Press, 1994) 서적 중의 소개문을 번역한 것입니다.

• IMMUNE NETWORK
• /
• 제12권5호
• /
• pp.165-175
• /
• 2012

Vaccination is one of the most effective methods available to prevent infectious diseases. Mucosa, which are exposed to heavy loads of commensal and pathogenic microorganisms, are one of the first areas where infections are established, and therefore have frontline status in immunity, making mucosa ideal sites for vaccine application. Moreover, vaccination through the mucosal immune system could induce effective systemic immune responses together with mucosal immunity in contrast to parenteral vaccination, which is a poor inducer of effective immunity at mucosal surfaces. Among mucosal vaccines, oral mucosal vaccines have the advantages of ease and low cost of vaccine administration. The oral mucosal immune system, however, is generally recognized as poorly immunogenic due to the frequent induction of tolerance against orally-introduced antigens. Consequently, a prerequisite for successful mucosal vaccination is that the orally introduced antigen should be transported across the mucosal surface into the mucosa-associated lymphoid tissue (MALT). In particular, M cells are responsible for antigen up-take into MALT, and the rapid and effective transcytotic activity of M cells makes them an attractive target for mucosal vaccine delivery, although simple transport of the antigen into M cells does not guarantee the induction of specific immune responses. Consequently, development of mucosal vaccine adjuvants based on an understanding of the biology of M cells has attracted much research interest. Here, we review the characteristics of the oral mucosal immune system and delineate strategies to design effective oral mucosal vaccines with an emphasis on mucosal vaccine adjuvants.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
• /
• pp.92.2-92.2
• /
• 2013

The design and chemical synthesis of multifunctional nanomaterials have been providing potential applications in biomedical fields such as molecular imaging and drug delivery. Recently, bio-derived and/or synthetic nanostructured materials capable of modulating the immune system have been also issues of interest in immunology-related nanomedicine fields. In this talk, the recent research results on the development of nanostructured materials for enhanced immunity would be presented. I will introduce the chemical strategy for the combination of nanostructured materials and bioactive compounds to improve both anti-cancer immunity and vaccine delivery efficiency.

• 적정기술학회지
• /
• 제6권2호
• /
• pp.94-102
• /
• 2020

신재생에너지는 개발도상국의 농촌 지역의 지속가능한 개발을 위한 필수 요소이다. 나아가, 독립형 신재생에너지 발전 시스템을 의료 분야에 응용하여 삶의 질을 증진시킬 수 있다. 본 연구에서는 개발도상국에서 백신을 안전하게 보관하고 보급하여, 콜드체인 영역을 확장하기 위해서 신재생에너지 기반 백신 공급 시스템을 구축하였다. 백신 공급 시스템은 신재생 에너지로 전기를 공급하는 보건소와 이를 연결하는 스마트 백신 캐리어로 구성되었다. 백신 캐리어는 펠티어 소자를 이용하여 냉각 시스템을 개선하였고, 위치 및 온도 모니터링 장비를 설치하였다. 또한, 네팔의 남부 마을에서 현장시험과 지형학적 분석으로 개발된 시스템의 성능 및 효과를 평가하였다. 신재생에너지 시스템은 보건소의 냉장고를 정상적으로 작동시켰으며, 백신 캐리어는 더욱 긴 냉장 시간과 안정적인 온도 조절 성능을 확보하였다. 지형학적 분석 결과, 본 시스템이 기존보다 넓은 지역으로 백신을 보급할 수 있을 것으로 추정하였다. 따라서, 신재생에너지와 스마트 기기의 도입이 콜드체인 영역을 확장하고 백신 공급율을 증가시켜, 결과적으로 개발도상국의 농촌 지역 의료 서비스를 개선할 수 있을 것으로 기대한다.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2000년도 춘계총회 및 학술대회
• /
• pp.214.2-215
• /
• 2000