KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Studies on Developing Direct Gene Transfer Based on Naked Plasmid DNA for Treating Anemia

Naked Plasmid DNA를 이용한 빈혈 치료용 Direct Gene Transfer 시스템의 개발에 대한 연구

  • Park Young Seoub (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Jung Dong Gun (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Choi Cha Yong (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University)
  • 박영섭 (서울대학교 공과대학 협동과정 생물화학공학전공) ;
  • 정동건 (서울대학교 공과대학 협동과정 생물화학공학전공) ;
  • 최차용 (서울대학교 공과대학 협동과정 생물화학공학전공)
  • Published : 2004.10.01
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Abstract

Several gene delivery therapies are being developed for treatment of serum protein deficiency. EPO is one of the most promising therapeutic agent for this treatment which is currently being investigated in depth. This study has the ultimate purpose of improving the gene delivery system for an increase of red blood cell production. A plasmid DNA was constructed smaller than other plasmids for an increase in penetration into animal cells, and two genes were cloned into each vector as a co-delivery system to express erythropoietin, and interluekin-3 or thrombopoietin, which can act on erythroid cell, thus activating hematopoiesis synergically. This co-delivery system has an advantage of decreasing the labour required for industrial production of DNA vaccine. A new plasmid vector, pVAC, in size 2.9 kb, was constructed with the essential parts from PUC 19 and pSectagB, which is much smaller than other plasmid vector and is the size of 2.9 kb. Co-delivery system was constituted by cloning human erythropoietin with each of human interluekin-3 gene or human thrombopoietin gene into both pVAC and pSectagB. As a result, the transfection efficiency of pVAC was higer than that of pSectagB in vitro, and hematocrit level of the mice injected with pVAC is higher than that of other mice. And co-delivery system, made of several plasmid DNAs, was expressed in vitro.

Keywords

References

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