Browse > Article

Studies on Developing Direct Gene Transfer Based on Naked Plasmid DNA for Treating Anemia  

Park Young Seoub (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University)
Jung Dong Gun (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University)
Choi Cha Yong (Interdisciplinary Programs for Biochemical Engineering and Biotechnology, Seoul National University)
Publication Information
KSBB Journal / v.19, no.5, 2004 , pp. 341-347 More about this Journal
Abstract
Several gene delivery therapies are being developed for treatment of serum protein deficiency. EPO is one of the most promising therapeutic agent for this treatment which is currently being investigated in depth. This study has the ultimate purpose of improving the gene delivery system for an increase of red blood cell production. A plasmid DNA was constructed smaller than other plasmids for an increase in penetration into animal cells, and two genes were cloned into each vector as a co-delivery system to express erythropoietin, and interluekin-3 or thrombopoietin, which can act on erythroid cell, thus activating hematopoiesis synergically. This co-delivery system has an advantage of decreasing the labour required for industrial production of DNA vaccine. A new plasmid vector, pVAC, in size 2.9 kb, was constructed with the essential parts from PUC 19 and pSectagB, which is much smaller than other plasmid vector and is the size of 2.9 kb. Co-delivery system was constituted by cloning human erythropoietin with each of human interluekin-3 gene or human thrombopoietin gene into both pVAC and pSectagB. As a result, the transfection efficiency of pVAC was higer than that of pSectagB in vitro, and hematocrit level of the mice injected with pVAC is higher than that of other mice. And co-delivery system, made of several plasmid DNAs, was expressed in vitro.
Keywords
DNA vaccine; plasmid DNA; human erythropoietin (hEPO); human interleukin3 (hIL-3); human trombopoietin (hTPO); in vitro transfection; in vivo; hematocrit;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Maruyama, H., K. Ataka, F. Gejyo, N. Higuchi, Y. Ito, H. Hirahara, I. Imazeki, M. Hirata, F. Ichikawa, T. Neichi, H. Kikuchi, M. Sugawa, and J. Miyazaki (2001), Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats, Gene Ther. Mar. 8(6), 461-468
2 Klinman, D. M., J. Conover, J. M. Leiden, A. S. Rosenberg, and J. M. Sechler (1999), Safe and effective regulation of hematocrit by gene gun administration of an erythropoietin-encoding DNA plasmid, Hum Gene Ther. Mar. 1 10:4 659-665
3 Martinez, X., C. Brandt, F. Saddallah, C. Tougne, C. Barrios, F. Wild, G. Dougan, P. Lambert, and C. Siegrist (1997), DNA immunization circumvents deficient induction of T helper type 1 and cytotoxic T lymphocyte responses in neonates and during early life, Proc. Natl. Acad. Sci. USA 94, 8726-8731
4 Guiherme, N., M. Ferreira, Gabriel A. Monteiro, and Joaquim M. S. Cabral (2000), TIBTECH. 18, 380-388
5 Nguyen, H. K., P. Lemieux, S. V. Vinogradov, C. L. Gebhart, N. Guerin, G. Paradis, T. K. Bronich, V. Y. Alakhov, and A. V. Kabanov (2000), Evaluation of polyether-polyethyleneimine graft copolymers as gene transfer agents, Gene Ther. 7, 126-138
6 Mennuni, C., F. Calvaruso, I. Zampaglione, G. Rizzuto, D. Rinaudo, E. Dammassa, G. Ciliberto, E. Fattori, and N. La Monica (2002), Hyaluronidase increases electrogene transfer efficiency in skeletal muscle, Hum. Gene. Ther. 13, 355-13365
7 Perrie, Y., P. M. Frederik, and G. Gregoriadis (2001), Liposome- mediated DNA vaccination: the effect of vesicle composition, Vaccine. Apr. 30, 3301-3310
8 Cheung, J. Y., and B. A. Miller (2001), Molecular mechanisms of erythropoietin signaling, Nephron. Mar. 87:3, 215-222
9 Mir, L. M., M. F. Bureau, J. Gehl, R. Rangara, D. Rouy, J. M. Caillaud, P. Delaere, D. Branellec, B. Schwartz, and D. Scherman (1999), High-efficiency gene transfer into skeletal muscle mediated by electric pulses, Proc. Natl. Acad. Sci. USA. 96, 4262-4267
10 Mennuni, C., F. Calvaruso, I. Zampaglione, G. Rizzuto, D. Rinaudo, E. Dammassa, G. Ciliberto, E. Fattori, and N. L. Monica (2002), Hyaluronidase Increases Electrogene Transfer Efficiency in Skeletal Muscle, Hum. Gene Ther. 13, 355-365
11 Maruyama, H, K. Ataka, F. Gejyo, N. Higuchi, Y. Ito, H. Hirahara, I. Imazeki, M. Hirata, F. Ichikawa, T. Neichi, H. Kikuchi, M. Sugawa, and J. Miyazaki. (2001), Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats, Gene Ther. 8(6), 461-468
12 Maruyama, H., M. Sugawa, Y. Moriguchi, I. Imazeki, Y. Ishikawa, N. Higuchi, J. Kazama, F. Gejyo, and J. Miyazaki (2000), Continuous Erythropoietin Delivery by Muscle-Targeted Gene Transfer Using in Vivo Electroporation, Hum. Gene Ther. 11, 429-437
13 Luo, D. and W. Mark Saltzman (2000), Nature Biotech. 18, 33-37
14 Tripathy, S. K., E. C. Svensson, H. B. Black, E. Goldwasser, M. Margalith, P. M. Hobart, and J. M. Leiden (1996), Long-term expression of erythropoietin in the systemic circulation of mice after intramuscular injection of a plasmid DNA vector, Proc. Natl. Acad. Sci. USA. 93, 10876-10880