• Title/Summary/Keyword: undiscovered taxa

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Phylogenetic Status of an Undiscovered Zygomycete Species, Syncephalastrum monosporum, in Korea

  • Duong, Tham Thi;Nguyen, Thi Thuong Thuong;Jeon, Sun Jeong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.371-376
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    • 2016
  • During a survey of undiscovered taxa in Korea, two zygomycete fungal isolates, EML-BT5-1 and EML-BT5-2, were isolated from the seed of a pumpkin (Cucurbita pepo) fruit in Korea. Based on their morphological characteristics and a sequence analysis of four genes, ITS1-5.8S-ITS2, 18S, 28S rDNA, and EF-$1{\alpha}$, the isolates were confirmed to be Syncephalastrum monosporum in the family Syncephalastraceae. To our knowledge, the zygomycete fungal species S. monosporum has not been previously described in Korea.

Characterization of Two New Records of Zygomycete Species Belonging to Undiscovered Taxa in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Seo Hee;Bae, Sarah;Jeon, Sun Jeong;Mun, Hye Yeon;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.1
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    • pp.29-37
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    • 2016
  • During a biodiversity survey of undiscovered taxa in Korea, two zygomycetous fungal strains were isolated. The first strain, EML-FSDY6-1 was isolated from a soil sample collected at Dokdo Island in the East Sea of Korea in 2013, and the second strain, EML-DG-NH3-1 was isolated from a rat dung sample collected at Chonnam National University garden, Gwangju, Korea in 2014. Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer, 18S and 28S rDNA, actin and translation elongation $factor-1{\alpha}$ genes. EML-FSDY6-1 and EML-DG-NH3-1 isolates were confirmed as zygomycete species, Absidia pseudocylindrospora and Absidia glauca, respectively. Neither species has previously been described in Korea.

Isolation and Characterization of Two Rare Mucoralean Species with Specific Habitats

  • Lee, Seo Hee;Nguyen, Thuong T.T.;Lee, Hyang Burm
    • Mycobiology
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    • v.46 no.3
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    • pp.205-214
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    • 2018
  • The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer and large subunit ribosomal DNA, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically, and mycologically significant to find such rare zygomycetous fungi in such specific habitats.

Isolation and Characterization of Three Unrecorded Zygomycete Fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans

  • Nguyen, Thuong T.T.;Choi, Young-Joon;Lee, Hyang Burm
    • Mycobiology
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    • v.45 no.4
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    • pp.318-326
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    • 2017
  • In a survey of undiscovered taxa in Korea, three zygomycete fungal strains-EML-W31, EML-HGD1-1, and EML-RUS1-1-were isolated from freshwater, grasshopper fecal, and soil samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of internal transcribed spacer and 28S rDNA, the isolates of EML-W31, EML-HGD1-1, and EML-RUS1-1 were confirmed to be Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans, respectively. These species have not been previously described in Korea.

Molecular and Morphological Confirmation of Three Undescribed Species of Mortierella from Korea

  • Nguyen, Thuong T.T.;Park, Se Won;Pangging, Monmi;Lee, Hyang Burm
    • Mycobiology
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    • v.47 no.1
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    • pp.31-39
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    • 2019
  • Three fungal isolates designated as CNUFC-YR329-1, CNUFC-PTS103-1, and CNUFC-PTS2-1 were discovered during a survey of fungal diversity of the order Mortierellales from freshwater and pine tree rhizosphere soil samples in Korea. The strains were analyzed morphologically and phylogenetically based on the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA gene sequences. Based on their morphology and phylogeny, the three isolates were identified as Mortierella elongata, M. horticola, and M. humilis, respectively. To the best of our knowledge, M. elongata, M. horticola, and M. humilis, belonging to an undiscovered taxon, have not been previously described in Korea.

Phylogenetic Status of Two Undescribed Zygomycete Species from Korea: Actinomucor elegans and Mucor minutus

  • Nguyen, Thuong T.T.;Jung, Hee-Young;Lee, Youn Su;Voigt, Kerstin;Lee, Hyang Burm
    • Mycobiology
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    • v.45 no.4
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    • pp.344-352
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    • 2017
  • During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.

Characterization of a Zygomycete Fungus, Mortierella minutissima from Freshwater of Yeongsan River in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.346-349
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    • 2016
  • During a survey of fungal diversity of the order Mortierellales, a zygomycete strain, EML-YS717-1, was isolated from a freshwater sample collected from the Yeongsan River in Gwangju, Korea. Based on its morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strain was identified as Mortierella minutissima. To the best of our knowledge, M. minutissima, has not previously been authentically reported in Korea.

Isolation and Characterization of Blakeslea trispora Isolated from Gut of Grasshopper and Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.355-359
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    • 2016
  • During a survey of fungal diversity in insect guts in Korea, two fungal strains, EML-PGH2 and EML-PUKI88, were isolated from the gut of grasshopper and soldier fly larvae inhabiting the bulrush plants at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strains were identified as Blakeslea trispora. To our knowledge, the zygomycete species B. trispora has not been previously described in Korea.

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong;Duong, Tham Thi;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.310-313
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    • 2016
  • While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.

Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

  • Nguyen, Thuong T.T.;Lee, Hyang Burm
    • Mycobiology
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    • v.46 no.4
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    • pp.317-327
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    • 2018
  • While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.