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http://dx.doi.org/10.4489/KJM.2016.44.4.371

Phylogenetic Status of an Undiscovered Zygomycete Species, Syncephalastrum monosporum, in Korea  

Duong, Tham Thi (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
Nguyen, Thi Thuong Thuong (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
Jeon, Sun Jeong (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
Publication Information
The Korean Journal of Mycology / v.44, no.4, 2016 , pp. 371-376 More about this Journal
Abstract
During a survey of undiscovered taxa in Korea, two zygomycete fungal isolates, EML-BT5-1 and EML-BT5-2, were isolated from the seed of a pumpkin (Cucurbita pepo) fruit in Korea. Based on their morphological characteristics and a sequence analysis of four genes, ITS1-5.8S-ITS2, 18S, 28S rDNA, and EF-$1{\alpha}$, the isolates were confirmed to be Syncephalastrum monosporum in the family Syncephalastraceae. To our knowledge, the zygomycete fungal species S. monosporum has not been previously described in Korea.
Keywords
Mucorales; Multigenes; Pumpkin seed; Syncephalastrum species; Undiscovered taxa;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Schroter J. Kryptogamen-Flora von Schlesien: Pilze. Breslau: J. U. Kern; 1908.
2 Benjamin RK. The merosporangiferous Mucorales. Aliso 1959;4:321-433.   DOI
3 Benny GL. The methods used by Dr. R. K. Benjamin, and other mycologists, to isolate Zygomycetes. Aliso 2008;26:37-61.   DOI
4 Ho HC, Shiau PF, Wu SL. Single-column purification of syncephapepsin:an aspartic proteinase from Syncephalastrum racemosum. Protein Expr Purif 1998;12:399-403.   DOI
5 Sapre MP, Jha H, Patil MB. Purification and characterization of a thermostabile-cellulase free xylanase from Syncephalastrum racemosum Cohn. J Gen Appl Microbiol 2005;51:327-34.   DOI
6 Wonganu B, Pootanakit K, Boonyapakron K, Champreda V, Tanapongpipat S, Eurwilaichitr L. Cloning, expression and characterization of a thermotolerant endoglucanase from Syncephalastrum racemosum (BCC18080) in Pichia pastoris. Protein Expr Purif 2008;58:78-86.   DOI
7 Amorim RV, Melo ES, Carneiro-da-Cunha MG, Ledingham WM, Campos-Takaki GM. Chitosan from Syncephalastrum racemosum used as a film support for lipase immobilization. Bioresour Technol 2003;89:35-9.   DOI
8 Yang S, Lee J, Kwak J, Kim K, Seo M, Lee YW. Fungi associated with the traditional starter cultures used for rice wine in Korea. J Kor Soc Appl Biol Chem 2011;54:933-43.   DOI
9 Pavlovic MD, Bulajic N. Great toenail onychomycosis caused by Syncephalastrum racemosum. Dermatol Online J 2006;12:7.
10 Schlebusch S, Looke DF. Intraabdominal Zygomycosis caused by Syncephalastrum racemosum infection successfully treated with partial surgical debridement and high-dose Amphotericin B lipid complex. J Clin Microbiol 2005;43:5825-7.   DOI
11 O'Donnell K, Lutzoni FM, Ward TJ, Benny GL. Evolutionary relationships among mucoralean fungi (Zygomycota): evidence for family polyphyly on a large scale. Mycologia 2001;93:286-97.   DOI
12 Hoffmann K, Pawlowska J, Walther G, Wrzosek M, de Hoog GS, Benny GL, Kirk PM, Voigt K. The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies. Persoonia 2013;30:57-76.   DOI
13 Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, Abdel-Wahab MA, Alvarado P, Alves-Silva G, Ammirati JF, Ariyawansa HA, et al. Fungal diversity notes 253-366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 2016;78:1-237.   DOI
14 Nguyen TT, Lee SH, Bae S, Jeon SJ, Mun HY, Lee HB. Characterization of two new records of zygomycete species belonging to undiscovered taxa in Korea. Mycobiology 2016;44:29-37.   DOI
15 Walther G, Pawlowska J, Alastruey-Izquierdo A, Wrzosek M, Rodriguez-Tudela JL, Dolatabadi S, Chakrabarti A, de Hoog GS. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 2013;30:11-47.   DOI
16 White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego:Academic Press; 1990. p. 315-22.
17 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9.   DOI
18 Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46.   DOI
19 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82.   DOI
20 Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
21 Zheng RY, Chen GQ, Hu FM. Monosporus varieties of Syncephalastrum. Mycosystema 1988;1:35-52.