• Journal of Korean Society of Environmental Engineers
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• v.35 no.7
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• pp.495-502
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• 2013

This work was performed to investigate proper condition of coagulation treatment as UF process pretreatment that consider UF permeate flux and residual Al concentration. The coagulant used an alum as $Al_2(SO_4)_3{\cdot}16H_2O$ and PACl (r = 1.5) made this study. The experiment was tested in adjusting conditions such as alum dose, flocculation time and coagulation pH of seawater. Consequently, higher coagulant dose lead to elevation of UF permeate flux while residual aluminium also increased in condition of pH 8.0. The most suitable condition which has a good permeate flux and low residual aluminium, in this works, was coagulant dose of 0.7 mg/L (as Al, alum) and 1.2 mg/L (as Al, PACl) and coagulation pH 6.5. In addition, applying the flocculation time with 1.2 mg/L of PACI reduced. The flocculation time reduced UF permeate flux in using alum.

• Microbiology and Biotechnology Letters
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• v.11 no.1
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• pp.47-52
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• 1983

The extracellular inulase produced by Aspergillus sp. C-58 was isolated by pH and charcoal treatment, precipitation with ammonium sulfate from the crude extract, and separated into 3 fractions (P-I, II, III) by DEAE-cellulose column chromatography in the ratio of 31.1:1.7:1 with respect to the activity. The ratio of inulase activity to sucrase activity of P-I, P-II and P-III fraction was 0.23, 0.24 and 1.1 respectively. The enzyme P-I fraction was purified 482 fold with a 22.8% yield by DEAE-Sephadex A-50, Sephadex G-75, Sephadex G-100 (1st and 2nd) column chromatography, and appeared homogeneous on polyacrylamide disc gel electrophoresis and ultracentrifugation.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.3
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• pp.429-439
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• 2000

Various wastewater treatment technologies were applied for decolorization and disposal of the wastewater containing the pigments, which consist of Lake Red C(Barium) or/and Lithol Rubine(Calcium) pigments. In an application of ozonation $COD_{Mn}$ was generally decreased with an increase of amounts of ozone applied, however, the decolorization effect was not that good except for Lithol Rubine series. In an application of Fenton oxidation and electrochemical process, a good $COD_{Mn}$ removal effect for all the pigment wastewater and a slight decolorization effect for a part of Lithol Rubine series were observed. In an application of ultra filtration(UF) and reverse osmosis(RO), an excellent $COD_{Mn}$ removal and decolorization(almost 100%) effects of all the pigment wastewater were observed. Thus the water treated by the UF and RO could be reusable and thus save operating costs of the pigment manufacturing plants.

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.751-756
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• 2009

In the perspective of saving energy in buildings, the high performance of insulation and air tightness for improving the heating and the cooling efficiency, has brought economically positive effects. However, these building energy saving technologies cause the lack of ventilation, which is the direct cause of increasing the indoor contaminants, and is also very harmful to the residents, because they spend over 90% of their time indoors. Therefore, the ventilation is important to keep the indoor environment clean and it can also save the energy consumption. In this study, a HEPA type nano ceramic filter is designed as a passive ventilation system to collect airborne particles and to supply fresh outdoor air. The double layer filter, which has $30{\mu}m$ in diameter at the conditions of 10wt% of concentration and 3kV/cm of the electric intensity, is produced by electrospinning. The filtration coating technology is confirmed in the solution with $SiO_2$ nano particles using polymer nano fibers. Also double layer filters are coated with $SiO_2$ nano particles and finally the porous construction materials are made by sintering in the electric furnace at $200{\sim}1400^{\circ}C$. The efficiency is measured 96.67% at the particle size of $0.31{\mu}m$, which is slightly lower than HEPA filter. However the efficiency is turned out to be sufficient.

• KSBB Journal
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• v.18 no.3
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• pp.180-185
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• 2003

Glycosaminoglycans (GAGS was extracted from sea squirt, Styela clava with sodium phosphate at 105$^{\circ}C$ for 2 hr and deproteinized with trichloroacetic acid or hydrochloride. The GAGs obtained from tunic consist 41.7% crude carbohydrates, 31.8% crude protein, and 31.2% sulfate. It was mainly constituted of galactose, glucosamine, glucose, mannose, and glacrosamine. The prominent amino acid were phenylalanine, threonine, glutamic acid, and aspartic acid. Mineral contents was mainly constituted 3.0 mg% sodium, 1.6 mg% potassium, and 1.2 mg% phosphorus. Trichloroacetic acid, hydrochloride and 5-sulfosalicylic acid were used for deprotein of the GAGs. Effective volume for deprotein of crude GAGs were 5.0% trichloroacetic acid (w/v) and 10.0% HCI (v/v) treatment. The deproteinized GAGs contained 35.1%, 35.4% of protein and 22.0%, 18.5% of sulfate, respectively.

• Korean Chemical Engineering Research
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• v.52 no.1
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• pp.98-105
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• 2014

Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and $25{\sim}40^{\circ}C$ respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T $25^{\circ}C$.

• Food Science and Preservation
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• v.20 no.3
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• pp.435-439
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• 2013

As byproducts of chicken slaughtering, chicken feathers are produced and mostly discarded without proper treatment, which results in serious environment pollution. Therefore, the appropriate treatment and utilization of chicken feathers are needed. In particular, chicken feathers can be used as protein sources for the preparation of protein hydrolysates, considering that chicken feathers have a large amount of proteins. In this study, chicken feather protein hydrolysates were prepared and their iron-binding peptides were isolated. Chicken feather protein was extracted from feathers of slaughtered chicken, and its hydrolysates were prepared via hydrolysis with Flavourzyme for 8 h. Then the chicken feather protein hydrolysates were ultra-filtered to obtain small peptide fractions and fractionated using Q-Sepharose and Sephadex G-15 columns to isolate their iron-binding peptides. Two major fractions were produced from each of the Q-Sepharose ion exchange chromatography and the Sephadex G-15 gel filtration chromatography. Among the fractions, the peptide fraction with a high iron-binding activity level, F12, was isolated. These results suggest that chicken feather protein hydrolysates can be used as iron supplements.

• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.1067-1070
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• 1997

Imunoglobulins from bovine plasma proteins were isolated by IMAC which $Cu^{2+}$ was chelated on a chelating sepharose fast flow gel. Most plasma proteins were eluted by 1st (0.01 M $Na_2HPO_4$, 0.5 M NaCl, pH 4.0) and 2nd elution buffers (0.01 M imidazol). According to the reverse phase HPLC analysis, it was found that proteins which were eluted by 1st elution buffer were mainly composed of serum albumin, while most IgG and transferrin were eluted by 2nd elution buffer. When protein fractions obtained by 2nd elution buffer was applied to ultra filtration system (molecular weight cut off: 100 kD), IgG was further purified. These results indicate that IMAC is an excellent tool for isolating imunoglobulins from plasma proteins.

• Membrane Journal
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• v.17 no.4
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• pp.375-380
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• 2007

To recover sericin protein from by-product in silk production process, a polyethersulfone hollow fiber ultra-filtration membrane module was used. The soap in the degummed solution was precipitated by calcium chloride. The influence of membrane module of submerged and external type on membrane fouling was investigated. The effect of soap and protein on the membrane fouling in the external type membrane module was also studied. The removal of soap resulted in decreasing the membrane fouling. It was shown that the protein and the membrane were affected by the soap.

• Journal of Korean Society on Water Environment
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• v.18 no.2
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• pp.151-157
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• 2002

Landfill leachate is one of highly contaminated and heterogeneous wastewater. The leachate from initial landfill can be treated by anaerobic process because it contains biodegradable matters, particularly, volatile fatty acids (VFAs). However, the anaerobic treatment of leachate is generally required longer hydraulic retention time (HRT) than aerobic process and another treatment process to satisfy effluent concentration. Therefore the modification of conventional anaerobic treatment is needed. Two phase anaerobic membrane process (TPAMP) is an integrated membrane process to be able to separate anaerobic metabolism into two phase which are acidogenesis and methanogenesis for improvement of anaerobic treatment efficiency. In this study, the efficiency of TPAMP and conventional anaerobic treatment were compared in terms of HRT, effluent SCOD, VFAs Membrane used in TPAMP was the UF of capillary type with the surface area of $0.048m^2$. The average effluent SCOD of conventional anaerobic treatment was 1352 mg/L and the removal was 96 % at HRT 60 days, while in TPAMP, 927 mg/L and 98% at HRT 30 days.