• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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• 2003.02a
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• pp.287-290
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• 2003

For microwave device applications, c-axis oriented high temperature superconducting YBa$_2$Cu$_3$O$_{7-{\delta}}$ (HTS-YBCO) epitaxial thin films on the r-cut sapphire substrate(Al$_2$O$_3$) were prepared. In order to reduce the lattice mismatch with a substrate and to enhance the crystallity of HTS thin films, CeO$_2$ buffer layer on the r-cut sapphire substrate was grown by the RF-magnetron sputtering. The YBCO films on the CeO$_2$ buffer layer were deposited using the pulsed-laser deposition (PLD) method. These HTS YBCO /CeO$_2$/Al$_2$O$_3$ multilayer thin films(30 $\times$ 30 mm$^2$) routinely exhibited a critical temperature(T$_{c}$) of 89 K from the R-T measurement. Using HTS YBCO/CeO$_2$ /Al$_2$O$_3$ multilayer thin film. We fabricated and characterized the microwave passive devices (planar type filters) with cryopack-age such as the coupled -line type low-pass filter (LPF) and the open-loop meander type bandpass filter (BPF).filter (BPF).).

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.1
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• pp.18-26
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• 2014

This study was conducted to analyze the recognition of the validity of the design changes that classified the type of design changes that occurred in the Apartment Complex Landscaping Planting Construction that have been ordered in 'A' work. This research was also carried out for the purpose of presentation of the basic data in order to effectively manage the change order of landscape-planting construction's future work. The following conclusions were derived. First, the 7 types of change orders in planting design were delineated as 'design changes for image improvement', 'design changes due to the mismatch of design statement', 'design changes due to lack of field study', 'design changes for consideration of the user', 'design changes due to consideration of the workability', 'the design changes for ease of maintenance', and the 'design changes due to the relationship with the Engineering species of other.' Second, it is possible to produce a negative impact of all construction costs have increased due to design changes of landscaping planting construction is grasped. Third, There were the most frequent change order in planting design for the 'image improving', 'due to the mismatch of planting design to the site situation.' Fourth, the results of confirmatory targeting professional landscaping areas, was found that the statistics of the type of change landscaping planting construction design seven derived in this study is valid.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.11
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• pp.94-100
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• 2014

In this paper, a design of low power Current-Reuse Voltage Controlled Oscillator (VCO) which has wide tuning range about 1.95 GHz ~ 3.15 GHz is presented. Class-C type is applied to improve phase noise and 2-Step Auto Amplitude Calibration (AAC) is used for minimizing the imbalance of differential VCO output voltage which is main issue of Current-Reuse VCO. The mismatch of differential VCO output voltage is presented about 1.5mV ~ 4.5mV. This mismatch is within 0.6 % compared with VCO output voltage. Proposed Current-Reuse VCO is designed using CMOS $0.13{\mu}m$ process. Supply voltage is 1.2 V and current consumption is 2.6 mA at center frequency. The phase noise is -116.267 dBc/Hz at 2.3GHz VCO frequency at 1MHz offset. The layout size is $720{\times}580{\mu}m^2$.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.208-216
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• 2002

Background: Reactive oxygen and nitrogen are produced by rheumatoid arthritis (RA) synovial tissue and can induce mutations in key genes. Normally, this process is prevented by a DNA mismatch repair (MMR) system that maintains sequence fidelity. Key members of the MMR system include MutS${\alpha}$ (comprised of hMSH2 and hMSH6), which can sense and repair single base mismatches and 8-oxoguanine, and MutS${\beta}$ (comprised of hMSH2 and hMSH3), which repairs longer insertion/deletion loops. Methods: To provide further evidence of DNA damage, we analyzed synovial tissues for microsatellite instability (MSI). MSI was examined by PCR on genomic DNA of paired synovial tissue and peripheral blood cells (PBC) of RA patients using specific primer sequences for 5 key microsatellites. Results: Surprisingly, abundant MSI was observed in RA synovium compared with osteoarthritis (OA) tissue. Western blot analysis of the same tissues for the expression of MMR proteins demonstrated decreased hMSH6 and increased hMSH3 in RA synovium. To evaluate potential mechanisms of MMR regulation in arthritis, fibroblast-like synoviocytes (FLS) were isolated from synovial tissues and incubated with the nitric oxide donor S-nitroso-N-acetylpenicillamine (SNAP). Western blot analysis demonstrated constitutive expression of hMSH2, 3 and 6 in RA and OA FLS. When FLS were cultured with SNAP, the RA synovial pattern of MMR expression was reproduced (high hMSH3, low hMSH6). Conclusion: Therefore, oxidative stress can relax the DNA MMR system in RA by suppressing hMSH6. Decreased hMSH6 can subsequently interfere with repair of single base mutations, which is the type observed in RA. We propose that oxidative stress not only creates DNA adducts that are potentially mutagenic, but also suppresses the mechanisms that limit the DNA damage.

• Journal of the Korea Convergence Society
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• v.11 no.7
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• pp.251-257
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• 2020

This study comparatively analyzed the differences in the talented human resources perceived by enterprises and job seekers in terms of recruitment trends of companies related to the 4th Industrial Revolution, focusing on 16 factors. The analysis data was collected from enterprises and job seekers related to the 4th Industrial Revolution, and the analysis method was applied to a convergence research methodology that mixes social network analysis and variance analysis using big data type. As a result, several things were verified. First, large enterprises emphasized communication, and small enterprises emphasized competency and confidence. Second, in the manufacturing industry, enterprises emphasized confidence and competence, and job seekers emphasized spec and passion. Third, in the service industry, enterprises emphasized personality and competence, and job seekers emphasized spec and global. Fourth, there was a big difference in talented human resources between enterprises and job seekers according to manufacturing and service industries. Based on these results, we discussed the opening of employment information for enterprises to reduce the recognition mismatch in the talented human resources.

• Molecules and Cells
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• v.21 no.1
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• pp.63-75
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• 2006

The 5′-region of Potato virus X (PVX) RNA, which contains an AC-rich, single-stranded region and stem-loop structure 1 (SL1), affects RNA replication and assembly. Using Systemic Evolution of Ligands by EXponential enrichment (SELEX) and the electrophoretic mobility shift assay, we demonstrate that SL1 interacts specifically with tobacco protoplast protein extracts (S100). The 36 nucleotides that correspond to the top region of SL1, which comprises stem C, loop C, stem D, and the tetra loop (TL), were randomized and bound to the S100. Remarkably, the wild-type (wt) sequence was selected in the second round, and the number of wt sequences increased as selection proceeded. All of the selected clones from the fifth round contained the wt sequence. Secondary structure predictions (mFOLD) of the recovered sequences revealed relatively stable stem-loop structures that resembled SL1, although the nucleotide sequences therein were different. Moreover, many of the clones selected in the fourth round conserved the TL and C-C mismatch, which suggests the importance of these elements in host protein binding. The SELEX clone that closely resembled the wt SL1 structure with the TL and C-C mismatch was able to replicate and cause systemic symptoms in plants, while most of the other winners replicated poorly only on inoculated leaves. The RNA replication level on protoplasts was also similarly affected. Taken together, these results indicate that the SL1 of PVX interacts with host protein(s) that play important roles related to virus replication.

• International Journal of Stem Cells
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• v.17 no.1
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• pp.59-69
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• 2024

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in clinical trials, where one of the most significant is graft rejection by the patient immune system in response to human leukocyte antigen (HLA) mismatch when transplants are obtained from an allogeneic (non-self) cell source. Homozygous SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical application and to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the efficient generation of neural precursor cells in hPSCs and also the differentiation of dopaminergic neurons. Additionally, there was no difference when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and hetero-SCNT-hESCs. We propose that there are no differences in the differentiation capacity and HLA expression among hPSCs that can be cultured in vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of applications when transplanted with neural precursor cells in the context of clinical trials.

• Journal of the Korean Vacuum Society
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• v.6 no.S1
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• pp.141-153
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• 1997

The paraelectric cubic-to-ferroelectric tetragonal phase transition of the thin Pb(Zr, Ti)$O_3$ (PZT) films grown on MgO(001) substrate was investigated in a series of synchrotron x-ray scattering experiments. As the thickness of the film decreases the transition temperature and the amount of the tetragonal distortion were decreased continuously Different from only the c-domains were existent in the thinnest 25nm thick film. Based on this we propose a model for the domain structure of the tetragonal PZT/MgO(100) film that is very different from the ones suggested in literature. We attribute the suppression of the transition to the substrate field that prefers the c-type domains near the interface and suppresses the tetragonal distortion to minimize the film-substrate lattice mismatch.

• Proceedings of the KIEE Conference
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• 2001.05a
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• pp.38-41
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• 2001

This paper presents a digital current differential protection algorithm for a transformer in power system. This algorithm uses an FIR filter to improve the performance of the relay. This paper presents a practical method setting the operating slope of the relay and reduce ct mismatch. A series of EMTP simulation results have shown effectiveness of the algorithm under various type of transformers and conditions.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.10 no.1
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• pp.94-100
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• 2001

Distortion is a potential problem with all welded fabrication and should be caused dimensional changes and mismatch of joints during welding fabrication. Correction unacceptable weld distortion is extremely costly and in some case impossible. The aim of the present work is to verify the variation of the compressive force, tensile force and distortion during plastic deformation under Tungsten-inert-gas(TIG) welding, on type 304 stainless steel. Experimental results show that possibili-ty if measuring deformation during welding and good correlation was found between analytical and experimental result ad finite element methods have been used to model temperature analysis.