• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.6
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• pp.1157-1163
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• 2002

This experimental research has been done to study the effects of Gmibojungikgi-tang(GBJIKT) on the anti-allergic reaction. We found the several important results from the research which has been performed by two experiments toward immediately type and delayed type in order to study the effects of GBJIKT on hypersensitivity response to mice. The results obtained from our research are as following: The survival rate of one group to which we injected only the compound 48/80 is almost 0% according to its density and timing test. In the other hand, the survival rates of the other group to which we injected both of the compound 48/80 and GBJIKT are 10%, 10%, 20%, 30%, 20%, and 40% according to 0.025, 0.05, 0.1, 0.25 0.5 and 1(mg/g) of compound 48/80. Time dependency test also shows the 0% survival rates in 5 and 10 minutes. GBJIKT revealed the significantly inhibitory effect on Compound 48/80 induced Mast cell degranulation. GBJIKT showed the significantly inhibitory effect in the delayed type hypersensitivity response to picry1 chloride GBJIKT showed the significantly inhibitory effect in the delayed type hypersensitivity response to sheep red blood cell. Our research provides the important evidence that GBJIKT is benificial to the prevention and treatment of allergy related diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.4
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• pp.1075-1081
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• 2003

This experimental research has been done to study the effects of Palmul-tang on the anti-allergic reaction. We found the several important results from the research which has been performed by two experiments toward immediately type and delayed type in order to study the effects of Palmul-tang on hypersensitivity response to mice. The results obtained from our research are as following: The survival rate of one group to which we injected only the compound 48/80 is almost 0% according to its density and timing test. In the other hand. the survival rates of the other group to which we injected both of the compound 48/80 and Palmul-tang are 20%, 0%. 40%, 10%, 20% and 50% according to 0.025, 0.05, 0.1, 0.25 0.5 and 1 (mg/g) of compound 48/80. Time dependency test also shows the 30% and 20% survival rates in 5 and 10 minutes. Palmul-tang revealed the significantly inhibitory effect on Compound 48/80 induced Mast cell degranulation. Palmul-tang showed the significantly inhibitory effect in the delayed type hypersensitivity response to picry1 chloride. Palmul-tang showed the significantly inhibitory effect in the delayed type hypersensitivity response to sheep red blood cell. Our research provides the important evidence that Palmul-tang is benificial to the prevention and treatment of allergy related aiseases.

• Journal of Life Science
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• v.13 no.1
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• pp.73-82
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• 2003

This experimental research has been done to study the effects of Bojungikgi-tang(BJIKT) on the anti-allergic reaction. We found the several important results from the research which has been performed by two experiments toward immediately type and delayed type in order to study the effects of BJIKT on hypersensitivity response to mice. The results obtained from our research are as following: 1. The survival rate of one group to which we injected only the compound 48/80 is almost 0% according to its density and timing test. In the other hand, the survival rates of the other group to which we injected both of the compound 48/80 and BJIKT are 20%, 10%, 30%, 10%, 40%, and 70% according to 0.025, 0.05, 0.1, 0.25 0.5 and 1(mg/g) of compound 48/80. Time dependency test also shows the 10% and 0% survival rates in 5 and 10 minutes. 2. BJIKT revealed the significantly inhibitory effect on Compound 48/80 induced Mast cell degranulation. 3. BJIKT showed the significantly inhibitory effect in the delayed type hypersensitivity response to picryl chloride. 4. BJIKT showed the significantly inhibitory effect in the delayed type hypersensitivity response to sheep red blood tell. Our research provides the important evidence that BJIKT is benificial to the prevention and treatment of allergy related diseases.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.4 no.1
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• pp.1-22
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• 1991

The object of this research is to find out the clinical effects of Sopungsan and Gamisopungsan on Immune response and the An1i-allergic reaction to rats and mice. The results obtained are as follows: 1. Both sopungsan and Gamisopungsan have a tendency to decrease on the delayed type hypersensitivity response in methotrexate treated mice, but are not recognized as having significance. 2. Both Sopungsan and Gamisopungsan reveal the increasing effects with significance on the hemagglution titer in mice. 3. Gamisopungsan reveals the increasing effect with significance on the hemolysin titer in mice. 4. Both Sopungsan and Gamisopungsan have a tendency to increase on the appearance of Rosette forming cells in mice, but are not recognized as having significance. 5. Both Sopungsan and Gamisopungsan reveal the increasing effects with significance on phagocytic index K and a in mice 6. Sopungsan reveals the decreasing effect, on the homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody aganist egg white albumin. 7. Gamisopungsan reveals the decreasing effect with significance on vascular permeabi1ity response to intradermal histamin in rats. 8. Sopungsan reveals the decreasing effect with significance, on vascular permeability response to intradermal serotonin in rats. 9. Both Sopungsan and Gamisopungsan reveal the decreasing effects with significance on the delayed type hypersensitivity response to picryl chloride in mice. 10. Both Sopungsan and Gamisopungsan reveal the decreasing effects with significance on the delayed type hypersensitivity response to sheep red blood cell in mice. According to the above results, Sopungsan and Gamisopungsan are concluded to have the increasing effect of immunity and anti-allergic reaction.

• The Journal of Pediatrics of Korean Medicine
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• v.4 no.1
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• pp.19-30
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• 1990

Experimental Studies were done to research the clinical effects of Hyunggaeyeugyotang on the Anti-allergic effect in mice and rats. The results obtained as follows; 1. In the effect of Hyunggaeyeungyotang on vascular permeability responses to intradermal histamine in rats, Hyunggaeyeugyotang revealed Significant effect. 2. In the effect of Hyunggaeyeungyotang on vascular permeability responses to intradermal serotonin in rats, Hyunggaeyeungyotang revealed significant effect. 3. In the 48hr homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody against egg albumin, Hyunggaeyeungyotang revealed significant effect. 4. In the effect of Hyunggaeyeungyotang on Delayed-type hypersensitivity responses to picryl chloride in mice, Hyunggaeyeungyotang revealed none of significant. 5. In the effect of Hyunggaeyeungyotang on Delayed-type hypersensitivity responses to SRBC in mice, Hyunggaeyeungyotang revealed none of significant. According to above-stated results, Hyunggaeyeungyotang is concluded to be effective as anti-allergic regimen and recommended to be used for treatment of allergic disease.

• YAKHAK HOEJI
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• v.43 no.1
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• pp.98-103
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• 1999

Effects of Astragali Radix extract (AG) on the cell mediated-and nonlpecific immunotoxic responses of zinc chloride (Zn) were studied usign ICR mice. Mice were divided into 4 groups (10 mice/group), and Zn was given to the mice 1 hr after i.p. injection with 0.5g/kg of AG by i.p. injection daily for 10 days at a dose of 25 mg/kg. Immune responses on the responses on the relative weight of thymus, delayed-type hypersensitivity to SRBC (DTH), phagocytic activity and circulating leukocytes were evaluated. Zn treatment decreased body weight gain, the relative weight of thymus, DTH and circulating leukocytes compared with those in controls. AG treatment increased DTH, phagocytic activity and circulating leukocytes compared with those in controls. Combination of AG and Zn increased DTH and circulating leukocytes compared with those in controls, but decreased body weight gain and the relative weight of thymus. These findings indicated that AG decreased immunotoxicity of Zn on the DTH and circulating leukocytes.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.126.1-126.1
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• 2003

Compound-A is a phenylpropanoid isolated from Arctium lappa fruit. In this experiments, effect of Compound-A on sheep red bood cells (sRBC) - induced delayed type hypersensitivity (DTH) were studied in ICR male mice and determined the Rosette Forming Cells (RFC). Two weeks after sensitization of i.p. injection of sRBC (4$\times{10}^8$ cells), ICR male mice were challenged by i.p. injection of sRBC (2\times{10}^8$ cells). Five days the challenge of antigen, paw edema induced twenty-four hours after the last challenge by DTH. (omitted)

• The Journal of Pediatrics of Korean Medicine
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• v.6 no.1
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• pp.1-14
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• 1992

Experimental studies were done to research the clinical effects of Juguieum and Juguieum-gamibang(Juguieum with Lonicerae Flos and Forsythiae Fructus added) on the Antiallergic effect. The results obtained as follows; 1. In the effects of Jugieum and Jugieum-gamibang on vascular permeability responses to intradermal histamine, Juguieum group revealed none significant effect, but Jugieum-gamibang group revealed significant effect. 2. In the effects of Juguieum and Juguieum-gamibang on vascular permeability responses to intradermal serotonin, Juguieum and Juguieum-gamibang groups revealed significant effect. 3. In the 48hrs homologous passive cutaneous amaphylaxis provoked by the IgE-like antibody against egg albumin, Juguieum and Juguieum-gamibang groups revealed significant effect. 4. In the delayed type hypersensitivity responses to Picryl Chloride, Juguieum and Juguieum-gamibang groups revealed significant effect. 5. In the delayed type hypersensitivity responses to SRBC, Juguieum group revealed significant effect, but Jugieum-gamibang group revealed none significant effect. According to above-stated results, Juguieum and Juguieum-gamibang are concluded to be effective as anti-allergic regimen and recommended to be used for the treatment of type I and type IV allergic disease.

• The Journal of Korean Medicine
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• v.43 no.2
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• pp.117-123
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• 2022

Objectives: This study is to report a case of hypersensitivity vasculitis with petechiae improved by Jeoryoung-tang Methods: Petechiae was observed in the upper and lower extremities of the patient. The patient also complained of cystitis symptoms such as dysuria and residual urine. Since there were no other accompanying symptoms such as abdominal pain, bleeding, or joint pain, the patient was diagnosed with hypersensitivity vasculitis and Jeoryoung-tang was prescribed for about a month. Results: After administering Jeoryoung-tang for about a month, petechiae improved from 8 to 2 points, and cystitis symptoms also improved from NRS 7 to 2 points. Conclusions: Since it has been confirmed that Jeoryoung-tang was effective as the treatment of skin symptoms through this case, Jeoryoung-tang can be prescribed for patients with other type skin diseases. However, since it is difficult to generalize this single case, continuous follow-up studies should be conducted.

• Biomolecules & Therapeutics
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• v.28 no.5
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• pp.456-464
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• 2020

Mast cells (MCs) are systemically distributed and secrete several allergic mediators such as histamine and leukotrienes to cause type I hypersensitivity. Dasatinib is a type of anti-cancer agent and it has also been reported to inhibit human basophils. However, dasatinib has not been reported for its inhibitory effects on MCs or type I hypersensitivity in mice. In this study, we examined the inhibitory effect of dasatinib on MCs and MC-mediated allergic response in vitro and in vivo. In vitro, dasatinib inhibited the degranulation of MCs by antigen stimulation in a dose-dependent manner (IC₅₀, ~34 nM for RBL-2H3 cells; ~52 nM for BMMCs) without any cytotoxicity. It also suppressed the secretion of inflammatory cytokines IL-4 and TNF-α by antigen stimulation. Furthermore, dasatinib inhibited MC-mediated passive cutaneous anaphylaxis (PCA) in mice (ED₅₀, ~29 mg/kg). Notably, dasatinib significantly suppressed the degranulation of MCs in the ear tissue. As the mechanism of its effect, dasatinib inhibited the activation of Syk and Syk-mediated downstream signaling proteins, LAT, PLCγ1, and three typical MAP kinases (Erk1/2, JNK, and p38), which are essential for the activation of MCs. Interestingly, in vitro tyrosine kinase assay, dasatinib directly inhibited the activities of Lyn and Fyn, the upstream tyrosine kinases of Syk in MCs. Taken together, dasatinib suppresses MCs and PCA in vitro and in vivo through the inhibition of Lyn and Fyn Src-family kinases. Therefore, we suggest the possibility of repositioning the anti-cancer drug dasatinib as a treatment for various MC-mediated type I hypersensitive diseases.