• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1871-1880
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• 2016

Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the two-step pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

• Nuclear Engineering and Technology
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• v.35 no.1
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• pp.45-54
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• 2003

A hyperbolic two-phase, two-fluid equation system developed in the previous work has been implemented in an existing nuclear safety analysis code, MARS. Although the implicit treatment of interfacial pressure force term introduced in momentum equation of the hyperbolic equation system is required to enhance the numerical stability, it is very difficult to implement in the code because it is not possible to maintain the existing numerical solution structure. As an alternative, two-step approach with stabilizer momentum equations has been selected. The results of a linear stability analysis by Von-Neumann method show the equivalent stability improvement with fully-implicit solution method. To illustrate the applicability, the new solution scheme has been implemented into the best-estimate thermal-hydraulic analysis code, MARS. This paper also includes the comparisons of the simulation results for the perturbation propagation and water faucet problems using both two-step method and the original solution scheme.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.05a
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• pp.250-253
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• 2001

This paper describes the effect of step bilge on motion characteristics of FPSO. The purpose of the present study is to investigate motion responses of FPSO with two types of step bilge. Model test result shows that the performance of the bottom type step bilge, that is designed to avoid collision, is as good as that of the side type step bilge.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.7
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• pp.26-31
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• 1995

A new actuating mechanism suitable for a micro positioning device is developed using piezo-electric elements. The actuator can make a step movement of 0.5 .mu. m up to 3.5 .mu. m. The step size can be adjusted on demand. By repeating this step action, long distance movement is achieved. Precise positioning can be obtained by combining the coarse motion with the maximum step size and fine motion. Two types of fine motion have been proposed for a driving method. Firstly, feedback control bases on PID is applied. The experimental results for the two method are presented.

• Journal of Electrical Engineering and Technology
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• v.6 no.2
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• pp.280-283
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• 2011

In the present work, a new approach is proposed for via interconnects of semiconductor devices, where multi-wall carbon nanotubes (MWCNTs) are used instead of conventional metals. In order to implement a selective growth of carbon nanotubes (CNTs) for via interconnect, the buried catalyst method is selected which is the most compatible with semiconductor processes. The cobalt catalyst for CNT growth is pre-deposited before via hole patterning, and to achieve the via etch stop on the thin catalyst layer (ca. 3nm), a novel 2-step etch scheme is designed; the first step is a conventional oxide etch while the second step chemically etches the silicon nitride layer to lower the damage of the catalyst layer. The results show that the 2-step etch scheme is a feasible candidate for the realization of CNT interconnects in conventional semiconductor devices.

• Journal of Institute of Control, Robotics and Systems
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• v.15 no.8
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• pp.804-811
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• 2009

This paper deals with the relationship between zeros and step response of the second and third order LTI(Linear Time Invariant) SISO(Single-Input and Single-Output) systems. As well known, if a system has a single unstable zero, it shows the step response with undershoot and, on the other hand, a stable zero slower than the dominant pole causes the system to have the step response with overshoot. Generally, in the case of a system with two unstable real zeros, it is known to have B type undershoot[7]. But there are many complex cases of the step response extrema corresponding to zeros location in third order systems. This paper investigates the whole cases depending on DC gains of the additive equivalence systems and they are to be classified by the region of zeros which are related to the shape of the step response. Moreover, monotone nondecreasing conditions are proposed in the case of complex conjugate zeros as well as the case of two stable zeros.

• Journal of applied mathematics & informatics
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• v.38 no.5_6
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• pp.439-461
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• 2020

A step-up option is a newly developed financial instrument that simultaneously provides higher security and profitability. This paper introduces two step-up options: step-up type1 and step-up type2 options, and derives the option pricing formulas using the Laplace transform. We assume that the underlying equity price follows a regime-switching model that reflects the long-term maturity of these options. The option prices are calculated for the two types of funds, a pure stock fund composed of risky assets only and a mixed fund composed of stocks and bonds, to reflect possible variety in the fund underlying asset mix. The impact of changes in the model parameters on the option prices is analyzed. This paper provides information crucial to product developments.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2001.10a
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• pp.93-100
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• 2001

The three dimensional(3D) reconstruction from two dimensional(2D) image data is using in many fields such as RPD(Rapid Product Development) and reverse engineering. In this paper, the main step of 3D reconstruction is comprised of two steps : image processing step and B-spline surface approximation step. In the image processing step, feature points of each cross-section are obtained by means of several image processing technologies. In the B-spline surface approximation step, using the data of feature points obtained in the image processing step, the control points of B-spline surface are obtained, which are used for IGES file of 3D CAD model.

• Journal of Advanced Marine Engineering and Technology
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• v.23 no.1
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• pp.9-18
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• 1999

The present study is aimed to investigate flow characteristics of two-dimensional backward-fac-ing step by numerical approach. A convection conservation difference scheme based upon SOLA algorithm is used for the solution of the two-dimensional incompressible Navier-Stokes equations to simulate the laminar transitional and trubulent flow conditions at which the experimental data can be available for the backlward-facing step. The twenty kinds of reynolds number are used for the calculations. In an effort to demonstrate that the reported solutions are dependent on the mesh refinement computations are performed on seven different meshes of uniformly increasing refinement. also to investigate the result of inflow dependence two kinds of the inflow profile are chosen for the laminar flow. Irregular grid is adopted to minimize the errors on the satis-faction fo the discretized continuity. As criterion of benchmarking the result of numerical simula-tion reattachment lengthis used for the selected Reynolds numbers. The results of the present study prove the fact that the numerical predictions agree well with the experimental data and the flow characteristics are shown at the backward-facing step.