• Title/Summary/Keyword: two-probe method

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[ $^1H$ ] MR Spectroscopy of the Normal Human Brains: Comparison between Signa and Echospeed 1.5 T System (정상 뇌의 수소 자기공명분광 소견: 1.5 T Signa와 Echospeed 자기공명영상기기에서의 비교)

  • Kang Young Hye;Lee Yoon Mi;Park Sun Won;Suh Chang Hae;Lim Myung Kwan
    • Investigative Magnetic Resonance Imaging
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    • v.8 no.2
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    • pp.79-85
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    • 2004
  • Purpose : To evaluate the usefulness and reproducibility of $^1H$ MRS in different 1.5 T MR machines with different coils to compare the SNR, scan time and the spectral patterns in different brain regions in normal volunteers. Materials and Methods : Localized $^1H$ MR spectroscopy ($^1H$ MRS) was performed in a total of 10 normal volunteers (age; 20-45 years) with spectral parameters adjusted by the autoprescan routine (PROBE package). In all volunteers, MRS was performed in a three times using conventional MRS (Signa Horizon) with 1 channel coil and upgraded MRS (Echospeed plus with EXCITE) with both 1 channel and 8 channel coil. Using these three different machines and coils, SNRs of the spectra in both phantom and volunteers and (pre)scan time of MRS were compared. Two regions of the human brain (basal ganglia and deep white matter) were examined and relative metabolite ratios (NAA/Cr, Cho/Cr, and mI/Cr ratios) were measured in all volunteers. For all spectra, a STEAM localization sequence with three-pulse CHESS $H_2O$ suppression was used, with the following acquisition parameters: TR=3.0/2.0 sec, TE=30 msec, TM=13.7 msec, SW=2500 Hz, SI=2048 pts, AVG : 64/128, and NEX=2/8 (Signa/Echospeed). Results : The SNR was about over $30\%$ higher in Echospeed machine and time for prescan and scan was almost same in different machines and coils. Reliable spectra were obtained on both MRS systems and there were no significant differences in spectral patterns and relative metabolite ratios in two brain regions (p>0.05). Conclusion : Both conventional and new MRI systems are highly reliable and reproducible for $^1H$ MR spectroscopic examinations in human brains and there are no significant differences in applications for $^1H$ MRS between two different MRI systems.

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Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3 (Bacillus amyloliquefaciens DL-3의 carboxymethylcellulase를 재조합 균주 E. coli JM109/DL-3에서 생산하는 배지의 염 농도를 최적화하기 위한 통계학적 실험 방법의 비교)

  • Lee, You-Jung;Kim, Hye-Jin;Gao, Wa;Chung, Chung-Han;Lee, Jin-Woo
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1205-1213
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    • 2011
  • The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that $K_2HPO_4$ gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was $K_2HPO_4$ ("probe>F" less than 0.0001), whereas $K_2HPO_4$ and $MgSO_4{\cdot}7H_2O$ were significant for the production of CMCase. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

Comparison of Insecticidal Activity and Feeding Behavior of Nilaparvata lugens by Root Uptake Times against Fenobucarb and Imidacloprid (Fenobucarb와 Imidacloprid의 벼 뿌리 침지시간에 따른 벼멸구의 살충활성과 섭식행동비교)

  • Yang, Jeong-Oh;Cho, Sun-Ran;Kwon, Yun-Hee;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.14 no.2
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    • pp.175-182
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    • 2010
  • Two insecticides, fenobucarb (36, 12 ppm) and imidacloprid (0.7, 0.4 ppm) were treated on rice seedling roots by root uptake method with different dipping time (1, 4, 8, 12, 16, 20, 24 hrs). This study was performed to elucidate the correlation between insecticidal activity and feeding behavior of Nilaparvata lugens using EPG (electrical penetration graph). EPG waveforms are recorded for 4 hours and classified into six waveforms. In the correlation between root uptake and corrected mortality, both insecticides showed higher corrected mortality as higher doses and as longer root uptake times. In the analysis of waveforms, N. lugens showed longer nonprobe time but shorter phloem feeding time at a higher dose. It was also showed the same result as longer the root uptake times. Therefore, it showed the correlation between insecticidal activity by root uptake time and both EPG waveforms (non-probing time and phloem feeding time).

Practical use palette research of color name digitl search system (색이름 디지털 검색체계의 실용팔레트 연구)

  • 문은배
    • Archives of design research
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    • v.16 no.3
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    • pp.161-174
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    • 2003
  • Choice and use of color are very important field for designer. Present color sprang by central field of design business unlike past. Color is used mainly by three fields of sensitivity, administration, mind. But, do substantial design including all of three fields at use. Practical research field that is based on basic research when see as actuality of domestic color design is been behind real condition. Specially, color sensitivity field and color management field are very important field, it can speak that color name arid related area are most important among two. Because collar name includes sensitivity and color management. This research constructs correct data because investigate and analyze and search all compatible color names that is announced in existing or is recorded in public cosmopolitanly. As a result, it is to promise accuracy when produce creation of idea and result of design using color name. Examined laying stress on color that domestic data that is used in research is basis with Korean industrial Standard, connection literature, on-the-spot probe. International data investigated American ISCC-NBS to base. Other abroad color name data examined official data of each country all systematically with Japan, Europe. Findings about 11,000 basis color names and 33,000 application color names sorted collection. Collection method and classification system follow in international standard and arranged for user's tile convenience. Also, use frequency did laying stress on Munsell that is high color system so that can aid in industrial design business. Improved to write all international standard color values sue as RGB, CMYK, XYZ and can be applied all in each field of design. Is applying and get along with continuation improvement and development in homepage of present KIDP, it may become more worth research.

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Development of a Water Sampling System for Unmanned Probe for Improvement of Water Quality Measurement (수질측정 방법 개선을 위한 무인 탐사체의 채수장치 개발방안)

  • Jung, Jin Woo;Cho, Kwang Hee;Kim, Min Ji
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.35 no.6
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    • pp.527-534
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    • 2017
  • The purpose of this study is to develop unmanned equipment that can automatically move to the desired point and measure water quality at the correct depth. For this purpose, we constructed a water sampling lift and water sampling container, an unmanned vessel equipped with a VRS-GPS, an acoustic echo sounder, and a water quality sensor. Also, we developed an automatic navigation algorithm and program, an automatic water sampling program, and a water quality map generation program. As a result of the experiment in the detention pond, the unmanned vessel sailed along the planned route with an accuracy of about 93% within the error range of 3m. In addition, the water quality sensor installed in the lift was able to acquire the water quality of the target area in real time and transmit it to the server via wireless Internet, and it was possible to monitor the water quality of each site in real time. Through field experiments, the water sampling lift was able to control the desired length with an accuracy of about 94%. The stretch length accuracy experiment of the water sampling lift was impossible to measure directly in the water, so it was replaced land-based experiment. We also found some unstable problems due to the weight of the water sampling lift and the weight of the air compressor to operate the water container. Except these two problems, we accomplished purpose of this study. An automated water quality measurement method using an unmanned vessel can be used to measure the quality of water in a difficult to access area and to secure the safety of the worker.

Soft Magnetic Property Depending on thickness of Free Layer in CoFe/Cu/CoFe/IrMn Spin Valve Film (CoFe/Cu/CoFe/IrMn 스핀밸브 박막의 자유층 두께 감소에 따른 연자성 자기저항 특성 연구)

  • Choi, Jong-Gu;Go, In-Suk;Gong, Yu-Mi;Kim, Min-Ho;Park, Young-Suk;Hwang, Do-Guwn;Lee, Sang-Suk
    • Journal of the Korean Magnetics Society
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    • v.19 no.2
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    • pp.52-56
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    • 2009
  • Interlayer coupling field, coercivity, magnetoresitance ratio, and magnetic sensitivity depending on the thickness of free CoFe layer for the CoFe/Cu/CoFe/IrMn multilayer are investigated. In case of CoFe layer of $30\;{\AA}$ thickness for the CoFe(t)/Cu($25\;{\AA}$)/CoFe($60\;{\AA}$)/IrMn($80\;{\AA}$) multilayer with ferromagnet/non-magnet/ferromagnet structure induced by IrMn layer, the lowest coercivity and the highest magnetic sensitivity, which is contained soft magnetic property, are observed. On the other side, in case of CoFe layer of $90\;{\AA}$ thickness, there are the highest coercivity and the lowest magnetic sensitivity. The fabricated CoFe($30\;{\AA}$)/Cu($25\;{\AA}$)/CoFe($60\;{\AA}$)]/IrMn($80\;{\AA}$) spin valve device with $2{\times}8{\mu}m^2$ patterning size are measured by two probe method, which is selected the sensing current as the longitudinal direction and the easy axis as the transversal direction. The measuring magntoresistance ratio and magnetic sensitivity of GMR-SV device having the soft magnetic property are 3.0% and 0.3%/Oe, respectively.

Variations in Electrical Conductivity of CNF/PPy Films with the Ratio of CNF and Application to a Bending Sensor (탄소나노섬유의 함량에 따른 CNF/PPy 필름의 전기전도도 및 굽힘센서로 응용)

  • Kim, Cheol;Zhang, Shuai;Kim, Seon-Myeong
    • Composites Research
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    • v.23 no.3
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    • pp.31-36
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    • 2010
  • A new material, carbon-nanofiber/polypyrrole (CNF/PPy) composite films, with different CNF weight ratios were fabricated electrochemically. Compared to the fabrication process based on simple physical mixing, the flexibility of the new film has been improved much better than the previous similar material. Pure PPy films were also fabricated by the new electrochemical process for the comparison of difference. Several SEM images were taken at two locations (electrode-side and solution-side) and at the cross section of the samples. Electrical conductivity of the composite films was measured by the four-probe method. The conductivity of the pure PPy film 0.013cm thick was 79.33S/cm. The CNF/PPy composite film with 5% CNF showed a conductivity of 93S/cm. One with 10% CNF showed a conductivity of 126 S/cm. The conductivity of PPy improves, as the CNF weight ratio increases. The good conductivity of CNF/PPy composites makes them a candidate for a small bending actuator. A bending sensor consists of PPy and PVDF, which can be operated in the air, was designed and the bending deflection was calculated using FEM.

Effect of Ferrous Ion on the Formation of Exotoxin A from Pseudomonas sp. PY002 and Cloning of it's Gene (Pseudomonas sp. PY002에서 Exotoxin A의 생성에 미치는 철 이온의 영향과 Exotoxin A 유전자의 클로닝)

  • Choi, Sun-Ah;Kim, Ho-Sang;Choi, Ji-Young;Kang, Jeong-Suk;Kim, Chun-Sung;Kim, Duck-Lae;Kim, Young-Ju;Yeo, Myeong-Gu;Park, Yeol
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.7-12
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    • 1999
  • By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.

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Failure in the COG Joint Using Non-Conductive Adhesive and Polymer Bumps (감광성 고분자 범프와 NCA (Non-Conductive Adhesive)를 이용한 COG 접합에서의 불량)

  • Ahn, Kyeong-Soo;Kim, Young-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.14 no.1
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    • pp.33-38
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    • 2007
  • We studied a bonding at low temperature using polymer bump and Non-Conductive Adhesive (NCA), and studied the reliability of the polymer bump/Al pad joints. The polymer bumps were formed on oxidized Si substrates by photolithography process, and the thin film metals were formed on the polymer bumps using DC magnetron sputtering. The substrate used was AL metallized glass. The polymer bump and Al metallized glass substrates were joined together at $80^{\circ}C$ under various pressure. Two NCAs were applied during joining. Thermal cycling test ($0^{\circ}C-55^{\circ}C$, cycle/30 min) was carried out up to 2000 cycles to evaluate the reliability of the joints. The bondability was evaluated by measuring the contact resistance of the joints through the four point probe method, and the joints were observed by Scanning Electron Microscope (SEM). The contact resistance of the joints was $70-90m{\Omega}$ before the reliability test. The joints of the polymer bump/Al pad were damaged by NCA filler particles under pressure above 200 MPa. After reliability test, some joints were electrically failed since thinner metal layers deposited at the edge of bumps were disconnected.

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Characteristics of LSC coated Metallic Interconnect for Solid Oxide Fuel Cell (LSC가 코팅된 고체산화물 연료전지용 금속연결재의 특성 연구)

  • Pyo, Seong-Soo;Lee, Seung-Bok;Lim, Tak-Hyoung;Park, Seok-Joo;Song, Rak-Hyun;Shin, Dong-Ryul
    • Korean Chemical Engineering Research
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    • v.48 no.2
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    • pp.172-177
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    • 2010
  • This study reports the high-temperature oxidation kinetics, ASR(area specific resistance), and interfacial microstructure of metallic interconnects coated with conductive oxides in oxidation atmosphere at $800^{\circ}C$, The conductive material LSC($La_{0.8}Sr_{0.2}CoO_3$, prepared by Solid State Reaction) was coated on the Crofer22APU. The contact behavior of coating layer/metal substrate was increased by sandblast. The electrical conductivity of the LSC coated Crpfer22APU was measured by a DC two probe four wire method for 4000hr, in air at $800^{\circ}C$. Microstructure and composition of the coated layer interface were investigated by SEM/EDS. These results show that a coated LSC layer prevents the formation and growth of oxide scale such as $Cr_2O_3$ and enhances the long-term stability and electrical performance of metallic interconnects for SOFCs.