• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1996년도 춘계학술발표회논문집(2)
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• pp.227-232
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• 1996

The one-third calibrating relation by steady solution can cause large error when applied to an unsteady flow with large amplitude waves. Extended calibrating method, which can treat the normal convective contribution, is developed. The normal mass convective term is included into the 2-D mass transport equation by means of rms value and random function. The unknown shear rate is numerically determined by solving the 2-D mass transport equation inversely. This recovery method which predicts the unknown shear rate is constructed. It is found that it works very well without distortion. The inclusion of the normal convective term has a negligible effect on the mass transfer coefficient.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2008년도 춘계학술대회 논문집
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• pp.313-316
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• 2008

This paper presents the error factors of Fall-of-potential test method used in measurements of the grounding-system impedance. This test methods inherently can introduce two possible errors in the measurements of grounding-system impedance: (1) ground mutual resistance due to current flow through ground from the ground electrode to the current probe, (2) ac mutual coupling between the current test lead and the potential test lead. The errors of ground mutual resistances and ac mutual coupling are expressed by the equation in calculating grounding impedance. These equations were calculated by Matlab that is commercial tool using mathematical calculation. The results of calculation were applied to correct grounding impedance.

• 대한기계학회논문집B
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• 제26권12호
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• pp.1747-1755
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• 2002

A complete theoretical treatment of the photothermal displacement technique has been performed for thermal diffusivity measurement in semi-infinite solid materials. The influence of the parameters, such as radius and modulation frequency of the heating beam and the thermal diffusivity, was studied. Usually, thermal diffusivity was determined by the deformation angle and phase angle as the relative position between the heating and probe beams. In this study, we proposed the simple analysis method based on the real part of deformation gradient as the relative position between two beams. It is independent in the parameters such as power of heating beam, absorption coefficient, reflectivity, Poisson's ratio, and thermal expansion coefficient.

• 미생물학회지
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• 제42권2호
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• pp.89-94
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• 2006

본 연구는 치주질환 병인론 연구에 빈번히 사용되는 Prevotella intermedia ATCC 49046 균주를 특이적으로 검출 및 동정할 수 있는 PCR primer를 개발하기 위하여 시행하였다. P. intermedia ATCC 49046 유전체 DNA를 추출하고, Hind III 제한효소를 이용하여 무작위 클로닝법으로 유전체 DNA 절편을 얻었다. Southern blot 분석법을 이용하여 DNA 절편의 특이성을 조사하였고, chain termination 법을 이용하여 핵산염기서열을 결정하였다. 이를 바탕으로 PCR primer를 설계하고, P. intermedia ATCC 49046에 대한 균주 특이성 및 검출 한계(민감도)를 조사하였다. Southern blot 분석 결과 Pig6 DNA probe는 서양인에서 분리 동정된 P. intermedia 균주와만 hybridization하였고, 한국인에서 분리 동정된 P. intermedia균주들과는 반응이 없었다. Pig6 DNA probe는 813 bp의 핵산염기로 구성되어 있었으며, 이를 바탕으로 설계된 Pig6-F3와 Pig6-R3 primer 쌍에 의해서는 서양 균주에 특이적인 PCR산물이 증폭되었다. Pig6-60F와 Pig6-770R primer 쌍에 의해서는 P. intermedia ATCC 49046 유전체 DNA에서만 특이적인 PCR 산물이 증폭되었다. 두 가지 primer 쌍들 각각에 대한 P. intermedia 유전체 DNA량의 검출 한계를 알아보기 위한 민감도실험 결과 두가지 primer 쌍들 모두 4 pg (약2000마리)까지 검출 가능하였다. 이상의 연구결과를 종합하면, Pig6-60F와 Pig6-770R primer쌍은 P. intermedia ATCC 49046을 균주 특이적으로 동정할 수 있어, 이 균주의 보존적 측면에서 유용하게 이용될 수 있을 것으로 사료된다.

• Tuberculosis and Respiratory Diseases
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• 제76권5호
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• pp.245-248
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• 2014

Miliary tuberculosis (TB) is a rare extrapulmonary form of TB, and there have been only two reports of miliary TB associated with infection with multidrug-resistant (MDR)-TB pathogen in an immunocompetent host. A 32-year-old woman was referred to our hospital because of abnormal findings on chest X-ray. The patient was diagnosed with MDR-TB by a line probe assay and was administered proper antituberculous drugs. After eight weeks, a solid-media drug sensitivity test revealed that the pathogen was resistant to ethambutol and streptomycin in addition to isoniazid and rifampicin. The patient was then treated with effective antituberculous drugs without delay after diagnosis of MDR-TB. To the best of our knowledge, this is the first case of miliary TB caused by MDR-TB pathogen in Korea.

• 감성과학
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• 제8권4호
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• pp.385-391
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• 2005

화장품을 바를 때 피부와 손가락 사이에서 일어나는 마찰계수는 화장품의 주관적 평가에 많은 영향을 주는 요소이다. in-vivo상태에서 피부와 접촉자 간의 마찰계수는 접촉자의 모양이나 거칠기의 정도 그리고 누르는 하중에 따라 변할 수 있다. 본 실험에서는 리니어 모터와 다축 로드셀을 이용하여 여러 가지 접촉자를 in-vivo상태에서 직접 피부에 접촉시키고 실시간으로 마찰계수를 측정할 수 있는 장치를 개발하였다. 이 장치를 이용하여 피부와 접촉자 간의 마찰계수를 측정하고 그 데이터를 화장품을 바를 때의 전후와 비교$\cdot$분석하였다. 또한 화장품을 바를 때 주관적인 평가에서 이용되는 형용사를 조사하며 마찰계수와의 그 상관관계를 분석하였다. 그 결과, 피부와 접촉자 간의 마찰계수는 화장품의 종류와 접촉자의 성질에 따라 0.17-1.2사이에서 나타나며, 주관적인 평가 또한 화장품을 바른 후 시간의 경과함에 따라 변하며, 그것은 마찰계수와 관련이 있음을 확인할 수 있었다.

• 대한수의학회지
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• 제35권3호
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• pp.531-536
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• 1995

Salmonella species are the most prevalent etiologic agents of food-borne acute gastroenteritis. Direct isolation of bacteria from the contaminated food, stool and animal tissues has been used for the diagnosis of salmonellosis routinely. However, isolation of bacteria is time consuming work and not so highly sensitive. In recent years, improved methods of polymerization chain reaction(PCR) and probe hybridization technique have led to the developement of diagnostic assays which employ to detect various human and animal pathogenic bacteria. In this study, we have performed the polymerization chain reaction to detect Salmonella pullorum from tissues and stool samples of chickens with two specific primers, ST5 and ST8C. The target DNA fragment of PhoE gene was successfully amplified from liver, spleen, pancreas, heart, lung, ovary, oviduct and feces samples. The amplified DNA fragments were hybridized with Salmonella typhymurium TA3000 PhoE probe by Southern hybridization. The PCR to amplify the PhoE gene was highly rapid and sensitive method to detect Salmonella pullorum from tissues and stool samples.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.73-81
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• 1985

We have previously isolated OsMADS4 gene that is a member of the class B MADS box genes from rice. In this study, another member of the class B MADS box genes was isolated from rice flower by the yeast two-hybrid screening method using OsMADS4 as bait. RNA blot analyses revealed that the clone, OsMADS16, was expressed in the second and third whorls, whereas the OsMADS4 transcripts were present in the second, third, and fourth whorls. These expression patterns of the OsMADS16 and OsMADS4 genes are very similar with those of AP3 and PI, the class B genes of Arabidopsis, respectively. In the yeast two-hybrid system, OsMADS4 interacted only with OsMADS16 among several rice MADS genes investigated, suggesting that OsMADS4 and OsMADS16 function as a heterodimer in specifying sepal and petal identities. We have also isolated OsMADS6 gene using OsMADS1 as a probe. Both are members of the AGL2 MADS family. Various MADS genes that encode for protein-protein interaction partners of the OsMADS6 protein were isolated by the yeast two-hybrid screening method. A majority of these genes belong to the AGL2 family. Sequence Homology, expression pattern, and ectopic expression phenotypes indicated that one of the interaction partners, OsMADS14, appears to be homologous to API, the class A MADS gene of Arabidopsis.

• Asian-Australasian Journal of Animal Sciences
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• 제13권1호
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• pp.77-85
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• 2000

To investigate the practical assessing method of pork quality, 302 carcasses were selected randomly to represent commercial conditions and were probed at 24 hr postmortem (PM) by Danish Meat Quality Marbling (MQM), Hennessy Grading Probe (HGP), Sensoptic Resistance Probe (SRP) and NWK pH-K21 meter (NpH). Also, filter paper wetness (FPW), lightness (L*), ultimate pH (pHu), subjective color (SC), firmness/wetness (SF) and marbling scores (SM) were recorded. Each carcass was categorized as either PSE (pale, soft and exudative), RSE (Reddish-pink, soft and exudative), RFN (reddish-pink, firm and non-exudative) or DFD (dark, firm and dry). When discriminant analysis was used to sort carcasses into four quality groups the highest proportion of correct classes was 65% by HGP, 60% by MQM, 52% by NpH and 32% by SRP. When independent variables were combined to sort carcasses into groups the success was only 67%. When RSE and RFN groups were merged so that there were only three groups (PSE, RSE+RFN, DFD) differentiating by color MQM was able to sort the same set of data into the new set of three groups with 80% accuracy. The proportions of correct classifications for HGP, NpH and SRP were 75%, 61% and 35% respectively. There was a decline in predication accuracy when only two groups, exudative (PSE and RES) and non exudative (RFN and DFD) were sorted. However, when two groups designated PSE and non-PSE (RSE, RFN and DFD) were sorted then the proportion of correct classification by MQM, HGP, SRP and NpH were 87%, 81%, 71% and 66% respectively. Combinations of variables only increased the prediction accuracy by 1 or 2% over prediction by MQM alone. When the data was sorted into three marbling groups based on SM this was not well predicted by any of the probe measurements. The best prediction accuracy was 72% by a combination of MQM and NpH.

• 한국전자파학회논문지
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• 제25권5호
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• pp.559-575
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• 2014

본 논문에서는 4-포트 수평/수직 프로브 스테이션을 사용하여 수직 커넥터 핀의 특성 측정 시 사용되는 회로망 분석기의 교정용 키트(calibration kit)를 제안한다. 기존의 수평면에서 사용되는 교정키트를 사용하면, 교정 후 프로브 암의 변경 및 추가적인 장치가 필요하며, 이로 인한 시간 소요뿐 아니라, 주변 상황에 민감한 프로브 팁의 위치 변동에 기인한 정밀도 훼손의 위험이 있다. 본 논문에서는 이를 보완할 수 있는 4-포트 수평/수직 겸용 교정키트를 제시한다. 교정은 SOLT 방법을 이용하였으며, Short, Open, Load는 수평면에서 교정하며, Thru는 프로브의 위치에 따라서 교정기판의 같은 수평면에서 교정하거나 또는 교정기판의 위쪽, 아래쪽을 수직으로 연결하여 사용할 수 있도록 설계/제작하였다. 즉, 기판에 수직한 Thru를 교정할 때에는 교정키트를 수직으로 세워 키트 양면을 사용하여 교정할 수 있는 방법을 제안하였다. 제안한 교정키트에서의 SOLT 회로 반사/전달 특성을 기존 교정키트의 SOLT 회로의 반사/전달 특성과 비교 분석하였으며, 두 교정키트의 전달 특성은 길이 보정 후, 300 kHz부터 8.5 GHz까지 약 ${\pm}0.1$ dB의 차이가 있음을 확인할 수 있었다. 이와 같이 설계/제작된 4-포트 수평/수직 교정키트의 유용성을 입증하기 위하여 각각 수평 측정의 경우와 수직 측정의 경우의 두 가지 경우 사례 연구를 수행하였으며, 그 결과를 기존의 교정키트를 사용한 후 측정된 결과 데이터와 비교분석하였다.