• Title/Summary/Keyword: two component system

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Understanding of Extracellular Fumarate Induced dctA Gene Expression Profile Using GFP Reporter (GFP 리포터를 이용한 외부 푸마르산 유도 dctA 유전자 발현 특성 파악)

  • Irisappan, Ganesh;Ravikumar, Sambandam;Kim, Joo-Han;Hong, Soon-Ho
    • Korean Journal of Microbiology
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    • v.47 no.2
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    • pp.174-178
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    • 2011
  • In Escherichia coli, DcuS/R two-component system controls fumarate import and utilization related gene expression. To understand the dynamic response of the bacterium DcuS/R two-component system with respect to fumarate concentrations, DcuS/R induced dctA promoter was integrated with GFP reporter protein. Expression monitoring study using recombinant strain showed that dctA promoter was upregulated with 1 mM of fumarate in M9 minimal medium.

THE DEVELOPMENT AND ASSESSMENT STRATEGY OF A THERMAL HYDRAULICS COMPONENT ANALYSIS CODE (열수력 기기해석용 CUPID 코드 개발 및 평가 전략)

  • Park, I.K.;Cho, H.K.;Lee, J.R.;Kim, J.;Yoon, H.Y.;Lee, H.D.;Jeong, J.J.
    • Journal of computational fluids engineering
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    • v.16 no.2
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    • pp.30-48
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    • 2011
  • A three-dimensional thermal-hydraulic code, CUPID, has been developed for the analysis of transient two-phase flows at component scale. The CUPID code adopts a two-fluid three-field model for two-phase flows. A semi-implicit two-step numerical method was developed to obtain numerical solutions on unstructured grids. This paper presents an overview of the CUPID code development and assessment strategy. The governing equations, physical models, numerical methods and their improvements, and the systematic verification and validation processes are discussed. The code couplings with a system code, MARS, and, a three-dimensional reactor kinetics code, MASTER, are also presented.

Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interaction

  • Kim, Sook-Kyung;Han, Jin-Hee
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.113-116
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    • 2000
  • Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find and additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The results indicates that this system is a promising one, capable of selecting an interacting component.

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Nonlinear pH Control Using a Three Parameter Model

  • Lee, Jie-Tae;Park, Ho-Cheol
    • Transactions on Control, Automation and Systems Engineering
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    • v.2 no.2
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    • pp.130-135
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    • 2000
  • A two parameter model of a single fictitious weak acid with unknown dissociation constant has been successfully applied to design a neutralization system for many multi-component acid streams. But there are some processes for which above two parameter model is not satisfactory due to poor approxmation of the nonlinearity of pH process. Here, for etter control of wide class of multi-component acid streams, a three parameter model of a strong acid and a weak acid with unknown dissociation constant is proposed. The model approximates effectively three types of largest gain variation nonlinearities. Based on this model a nonlinear pH control system is designed. Parameters can eeasily estimated since their combinations appear linearly in the model equations and nonlinear adaptive control system may also be constructed just as with the two parameter model.

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Economic design of consecutive k-out-of-n : F system (Consecutive k-out-of-n : F 시스템의 경제적 설계)

  • Yun, Won-Young;Kim, Gue-Rae
    • Journal of Korean Institute of Industrial Engineers
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    • v.26 no.2
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    • pp.128-135
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    • 2000
  • This paper considers a consecutive k-out-of-n:F system when the failure of a component in the system induces higher failure rate of the preceding survivor. The reliability, mean time to failure(MTTF), and average failure number of a consecutive k-out-of-n:F system are obtained, when the failure of a component increases the failure rate of the survivor which is working just before the failed component. Then the optimal number of consecutive failed components to minimize this long run average cost rate can be obtained. An example is considered to calculate the reliability, MTTF and average failure number of the system. And two procedures that find the optimal number of consecutive failed components are studied. Then, various cases of system parameters are also studied.

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PHOTOMETRIC STUDY OF THE NEAR-CONTACT BINARY CN ANDROMEDAE

  • Lee Chung-Uk;Lee Jae-Woo
    • Journal of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.25-30
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    • 2006
  • We completed four color light curves of the near-contact binary CN And during three nights from September to December 2004 using the 61-cm reflector and BV RI filters at Sobaeksan Observatory. We determined four new times of minimum light (two timings for primary eclipse, two for secondary). Newly obtained BV RI light curves and the radial velocity curves from Rucinski et a1. (2000) were simultaneously analyzed to derive the system parameters of CN And. We used the semi-detached mode 4 of the 2003-version of the Wilson-Devinney binary model, and interpreted the asymmetry of the light curve by introducing two spots; a cool spot on the primary component and a hot spot on the secondary component. New photometric parameters are not much different from those of Cicek et a1. (2005), and it is considered that the system is in the era of broken contact. From the orbital period study with all available timings including our data, we found a continous period decrease with a rate of $P_{obs}=--1.82{\times}10^{-7}\;d\;yr^{-1}$ that can be explained with two possible mechanisms. We think the most likely cause of the period decrease is a thermal mass transfer from the primary to the secondary component, rather than angular momentum loss due to a magnetic stellar wind.

Development and Implementation of Measures for Structural and Reliability Importance by Using Minimal Cut Sets and Minimal Path Sets (최소절단집합과 최소경로집합을 이용한 구조 및 신뢰성 중요도 척도의 개발 및 적용)

  • Choi, Sung-Woon
    • Journal of the Korea Safety Management & Science
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    • v.14 no.1
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    • pp.225-233
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    • 2012
  • The research discusses interrelationship of structural and reliability importance measures which used in the probabilistic safety assessment. The most frequently used component importance measures, such as Birnbaum's Importance (BI), Risk Reduction (RR), Risk Reduction Worth (RRW), RA (Risk Achievement), Risk Achievement Worth (RAW), Fussel Vesely (FV) and Critically Importance (CI) can be derived from two structure importance measures that are developed based on the size and the number of Minimal Path Set (MPS) and Minimal Cut Set (MCS). In order to show an effectiveness of importance measures which is developed in this paper, the three representative functional structures, such as series-parallel, k out of n and bridge are used to compare with Birnbaum's Importance measure. In addition, the study presents the implementation examples of Total Productive Maintenance (TPM) metrics and alternating renewal process models with exponential distribution to calculate the availability and unavailability of component facility for improving system performances. System state structure functions in terms of component states can be converted into the system availability (unavailability) functions by substituting the component reliabilities (unavailabilities) for the component states. The applicable examples are presented in order to help the understanding of practitioners.

Simulation of a CIM Workflow System Using Parallel Virtual Machine (PVM)

  • Chang-Ouk Kim
    • Journal of the Korea Society for Simulation
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    • v.5 no.2
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    • pp.13-24
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    • 1996
  • Workflow is an ordered sequence of interdependent component data activities each of which can be executed on an integrated information system by accessing a remote information system. In our previous research [4], we proposed a distributed CIM Workflow system which consists of a workflow execution model called DAF-Net and an agent-based information systems called AIMIS. Given a component data activity, there needs an interaction protocol among agents which allocates the component data activity to a relevant information systems exist. The objective of this research is to propose and test two protocols: ARR(Asynchronous Request and Response)protocol and NCL(Negotiation with Case based Learning) protocol. To test the effectiveness of the protocols, we applied the PVM(Parallel Virtual Machine) software to simulate the distributed CIM Workflow system. PVM provides a distributed computing environment in which users can run different software processes in different computers while allowing communication among the processes.

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Reliability Equivalence of Two Non-identical Components

  • Mustafa, A.
    • International Journal of Reliability and Applications
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    • v.9 no.1
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    • pp.79-93
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    • 2008
  • The aim of this work is to generalize reliability equivalence techniques to apply them to a system consists of two independent and non-identical components connected in series(parallel) system, that have constant failure rates. We shall improve the system by using one component only. We start by establishing two different types of reliability equivalence factors, the survival reliability equivalence (SRE) and mean reliability equivalence (MRE) factors. Our second studies, introducing some applications for our studies in airports and our life. Also, we introduced some numerical results.

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Estimation of Bivariate Exponential Model under Censored Data

  • Cho, Kil-Ho;Kim, Young-Il
    • Journal of the Korean Data and Information Science Society
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    • v.14 no.4
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    • pp.751-758
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    • 2003
  • We consider a life testing experiment in which several two-component shared parallel systems are put on test, and the test is terminated at a predesigned experiment time. The bivariate data obtained from such a system-level life testing can be classified into three cases: 1) the case of failed two components with known failures times, 2) the case of censored two components, and 3) the case of one censored component and the other failed component of which the failure time might be known or unknown. In this thesis, the likelihood estimators for Freund's bivariate exponential life distribution under above censoring scheme are obtained. Results of comparative studies based on Monte Carlo simulation are presented.

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