• 한국가금학회지
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• 제11권1호
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• pp.19-25
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• 1984

국내에서 생산되는 정어리어분, 청어어분, 멸치어분, 명태부산물어분 그리고 취치부산물 어분의 일반조성분, 칼슘, 인, 그리고 아미노산 함유량을 측정하였다. 이들 어분의 몇가지 필수아미노산(valine, methionine, isoleucine, leucine)을 제외하고는 필수아미노산 진정이용율의 유의한 차이가 있었다. 어분의 종류별 필수아미노산 이용율은 멸치어분, 정어리어분, 쥐치부산물어분, 청어어분 그리고 명태부산물어분이 각각 95, 93, 91, 91%순위였다. 개별 필수아미노산의 진정이용율은 시험 어분들간에 유의성이 인정되었다. (P＜0.01). Lysine 의 진정이용율은 청어어분, 명태부산물어분 그리고 쥐치부산물 어분순으로 낮은 반면에 phenylalanine은 쥐치부산물과 명태부산물어분 순위로 가장 낮았다. 일반적으로 멸치어분은 모든 어분중에서 진정아미노산 이용율이 높게 나타났다.

• 한국가금학회지
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• 제20권4호
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• pp.189-196
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• 1993

TAAA(true amino acid availability)방법에 의해 단백질공급원들의 진정아미노산 이용율과 대사에너지가를 측정하기 위하여 시험원료당 수탉 3수씩을 공시하여 35시간 절식후 30g의 시험원료를 강제급이 한 다음 36시간 동안 분요를 채취하였다. 진정아미노산 이용율은 단백질원료간에 유의한 차이(P<0.01)가 있었는데 어분(96.1%)이 가장 높았고, 다음이 옥수수글루텐(91.2%), 채종박(88.8%), 대두박(88.7%), 육골분(87.2%), 캐놀라박(86.1%), 면실박(82.6%) 그리고 우모분(82.5%) 순이었다. TAAA방법에 의한 가용 Iysine 값은 chick bioassay(CBA)나 화학적 방법중 FDNB방법에 의해 얻어진 가용 Iysine 값과 고도(P<0.01)의 상관관계가 있었다. 진정대사에너지가는 옥수수글루텐(4,011 kcal/kg)이 가장 높았고 다음이 어분(3,906), 우모분(3,098), 대두박(3,007), 육골분(2,631), 캐놀라(2,326), 면실박(2,246) 그리고 채종박(2,120) 순이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1487-1494
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• 2003

Studies were conducted with intact White Leghorn roosters to determine the true metabolisable energy (TME) and the true amino acid availability (TAAA) in five dehulled and hulled soybean meals produced in China. 60 roosters, kept in individual cages, were fasted for 48 h and then tube-fed 50 g of one of experimental feedstuffs and their excreta was then collected for the subsequent 48 h period. Two separate collection periods were used with each meal being fed to 12 roosters. The birds were given a 15-day recovery period between collection periods. An additional 12 roosters were either fasted or fed a protein-free diet in order to estimate the extent of endogenous losses of energy and amino acids in excreta. The average values per bird for nitrogen loss, endogenous energy losses (EEL) and endogenous energy losses corrected to zero-nitrogen balance ($EEL_n$) were found to be 0.74 g, 47.0 kJ and 21.5 kJ, respectively. It was found that the TME and TAAA values of dehulled soybean meal were higher than those of hulled soybean meal. The TME and nitrogen-corrected TME metabolisable energy values of dehulled soybean meal were 10.58 and 10.74 MJ/kg, respectively, while the corresponding values for hulled soybean meal were 10.03 and 10.27 MJ/kg, respectively. The average indispensable and dispensable amino acid availability of dehulled soybean meal was 92.1 and 93.5%, compared with 89.3 and 91.4% for hulled soybean meal. Dehulled soybean meal would therefore appear to be superior to hulled soybean meal as a source of protein and energy for use in poultry rations.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.620-633
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• 1998

Methods developed for measuring digestibility and availability of amino acids in feedstuffs used in pig nutrition are reviewed. Digestibility is a proportion of an amino acid in a feed that is absorbed from the digestive tract and should be determined from the difference between the amount of amino acid consumed and passing the distal ileum. Techniques for ileal digesta sampling including various types of cannulas: a re-entrant, T-piece, IPV, IPVC and ileaorectal anastomosis are described and comparisons amongst these methods are presented. Other methodologies like mobile bag technique, in vitro assays and mathematical prediction method are also described. Significance and methodologies for measurement of endogenous nitrogen and amino acids losses at the distal ileum and their effect on the apparent and true nitrogen and amino acid digestibilities in feeds are discussed. Factors influencing the apparent and true amino acid digestibilities such as dry matter intake, protein, fibre and antinutritive compounds content in the diet are discussed. Amino acid bioavailability -the proportion of the total amino acid digested and absorbed in a form utilized in metabolism - measured by the growth assay may differ from its ileal digestibility. Chemical methods for determination of available lysine content in heat treated feeds are evaluated.

• 한국가금학회지
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• 제22권3호
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• pp.155-160
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• 1995

To evaluate the nutritive values of outdoor mass cultivated Spirulina platensis both chemical analysis and bioassay were carried out using adult cockerels. Blue-green algae, Spirulina platensis contained about 71g /l00g DM of crude protein with balanced amino acid profiles although methionine is liable to he limiting to animals. Compared to fish meal, calcium content and calcium : phosphorus ratio of the Spirulina were not suitable in terms of animal requirements. Reasonable amount of y-linolenic acid(C18: 3 $\omega$6) in Spirulina platensis draws a clinical attention due to its historically recognized pharmacotheraputic functions. Metabolizable energy contents of Spirulina were 3.67 and 3.11 mcal /kg DM for TMEn and AMFn, respectively, which therefore, can he a reliable energy source for poultry. True amino acid availabilities of essential amino acids of Spirulina platensis were higher than 90% for poultry, which is better than comparative ingredient like fish meal. Overall data from both chemical analysis and bioassay demonstrated that the Spirulina platensis could he a favorable protein feedstuffs for poultry.

• 한국가금학회지
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• 제31권4호
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• pp.255-263
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• 2004

본 연구는 호마박의 사료적 가치를 평가하고 산란계의 성적에 미치는 호마박의 첨가 효과를 구명하기 위해 수행하였다. 실험 1에서는 16수의 ISA-Brown 종계 수탉을 공시하여 강제 급여 방법에 의해 호마박의 진정 대사에너지(TME),질소 보정 진정 대사 에너지 (TMEn)및 진정 아미노산 이용률(TAAA)을 조사하였다. 호마박의 TME 및 TMEn은 각각 2.30 kca1/g및 1.99 kcal/g이었고, 15개 아미노산의 평균 이용률은 $76.93\%$로 나타났다. 실험 2에서는 48주령의 ISA-Brown산란계 90수를 3개 처리(대조구, 호마박 $5\%$, 호마박 $10\%$)로 나누어 4주간의 사양 실험을 실시하였다. 실험사료 내 호마박 첨가가 난 생산성 및 계란 품질에 미치는 영향을 조사하였다. 산란계 사료 내에 호마박을 $5\%$및 $10\%$첨가했을 때 난 생산성 및 생산된 계란의 품질에는 큰 영향을 미치지 않은 것으로 나타났다. 호마박 $10\%$ 첨가구에서 난황 내 Cl8:3 $\omega$3의 비율이 가장 높았으나, 통계적인 유의차는 인정되지 않았다. 혈청 내GOT, G-GT, BUN및 cholesterol 조성에 있어서도 처리간에 큰 차이는 발견되지 않았다. 본 연구에서는 호마박을 정확한 원료 평가를 거쳐 사용한다면 산란계 사료에 있어서 $10\%$까지 사용하여도 난 생산성 및 계란 품질을 저하시키지 않았으며, 공시계에게 어떠한 부정적인 영향도 미치는 않았다는 결과가 시사되었다.

• 한국가금학회지
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• 제35권4호
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• pp.381-389
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• 2009

본 연구는 아미노산 소화율과 육계의 생산성에 대하여 CDDGS의 효과를 평가하기 위해 실시하였다. <시험 1> CDDGS의 $TME_n$가는 $2,937{\pm}50.1\;kcal/kg$ 이었다. <시험 2> 공시계는 1일령 Ross종 육계 192수를 CDDGS 첨가 수준에 따라 총 4처리구, 처리당 4반복, 반복당 12수씩 완전 임의 배치하였다. 시험 사료는 육계 초기($0{\sim}2$주), 전기($2{\sim}4$주)와 후기($4{\sim}6$)의 기본 사료에 CDDGS를 0, 6, 12, 18% 첨가하였으며, ME가와 CP는 일정하게 유지하였다. 증체량에는 큰 차이가 없었으나, 섭취량은 CDDGS 비급여구가 급여구보다 증가하였다(P<0.05). CDDGS 처리구에서 인의 소화율이 크게 증가하였으며, 아미노산 소화율은 CDDGS 첨가구가 대조구에 비해 lysine, threonine, aspartic acid 및 glycine에서 낮게 나타났다(P<0.05). 이상의 결과로부터, CDDGS는 높은 ME가를 가지고 있으며, CDDGS 첨가구는 대조구에 비해 아미노산 소화율이 낮았으나, 육계 생산성에는 크게 영향을 미치지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.917-925
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• 2014

An accurate feed formulation is essential for optimizing feed efficiency and minimizing feed cost for swine and poultry production. Because energy and amino acid (AA) account for the major cost of swine and poultry diets, a precise determination of the availability of energy and AA in feedstuffs is essential for accurate diet formulations. Therefore, the methodology for determining the availability of energy and AA should be carefully selected. The total collection and index methods are 2 major procedures for estimating the availability of energy and AA in feedstuffs for swine and poultry diets. The total collection method is based on the laborious production of quantitative records of feed intake and output, whereas the index method can avoid the laborious work, but greatly relies on accurate chemical analysis of index compound. The direct method, in which the test feedstuff in a diet is the sole source of the component of interest, is widely used to determine the digestibility of nutritional components in feedstuffs. In some cases, however, it may be necessary to formulate a basal diet and a test diet in which a portion of the basal diet is replaced by the feed ingredient to be tested because of poor palatability and low level of the interested component in the test ingredients. For the digestibility of AA, due to the confounding effect on AA composition of protein in feces by microorganisms in the hind gut, ileal digestibility rather than fecal digestibility has been preferred as the reliable method for estimating AA digestibility. Depending on the contribution of ileal endogenous AA losses in the ileal digestibility calculation, ileal digestibility estimates can be expressed as apparent, standardized, and true ileal digestibility, and are usually determined using the ileal cannulation method for pigs and the slaughter method for poultry. Among these digestibility estimates, the standardized ileal AA digestibility that corrects apparent ileal digestibility for basal endogenous AA losses, provides appropriate information for the formulation of swine and poultry diets. The total quantity of energy in feedstuffs can be partitioned into different components including gross energy (GE), digestible energy (DE), metabolizable energy (ME), and net energy based on the consideration of sequential energy losses during digestion and metabolism from GE in feeds. For swine, the total collection method is suggested for determining DE and ME in feedstuffs whereas for poultry the classical ME assay and the precision-fed method are applicable. Further investigation for the utilization of ME may be conducted by measuring either heat production or energy retention using indirect calorimetry or comparative slaughter method, respectively. This review provides information on the methodology used to determine accurate estimates of AA and energy availability for formulating swine and poultry diets.

• 한국가금학회지
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• 제17권2호
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• pp.101-107
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• 1990

본시험은 Canola 전지종실과 Canola 박 및 Canola 유의 유효에너지가(AM 및 TME)와 아미노산 이용을(TAAA)를 측정하기 위하여 실시하였다. 본시험에는 단관백색레그혼종(S.C.W.C.) 성계수탉이 실시되었으며, AME시험에서는 측정사료인Canola 전지증실, Canola 박 Canola 박＋유(60:40)을 30% 그리고 Canola 유를 10% 배합한 4종의 시험사료와 기초사료를 각각 자유채식시켰고 예비시험기간은 3일 채분기간은 4일이었다. TME와 TAAA시험에서는 Canola 전지종실과 Canola 박은 단-사료로, Canola 박＋유와 Canola 유는 각각 기초사료에 30%와 10%의 비율로 혼합하여 각각 1일 30g을 24시간의 절식기간 후에 강제투여(force feeding)하였고 48시간동안 전분을 채취하였다. 모든 에너지가는 질소정정 에너지가로 환산하였는데, Canola 전지종실, Canola 박, Canola 유 및 Canola ＋유의 AMEn가는 각각 4,485, 1,984. 8,275 및 5,655 kcal/kg이었고 TMEn가는 각각 4,577, 2,103, 8,487 및 5,630kcal/kg으로 측정되어 Canola 박＋유는 Canola 전지종실보다 높은 에너지가를 나타냈으며(P＜0.01), 전반적으로 Canola전지종실은 Canola 박보다 높은 TAAA가를 나타냈다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1761-1768
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• 2005

Effects of alpha-galactosidase (GAL) on broiler corn-soybean meal diet was investigated. In experiment 1, sixty cockerels were allocated to five groups, including three enzyme treatments (GAL added at 0, 500, and 1,000 mg/kg diet), a nitrogen-free diet group and a fast group. The true nitrogen-corrected ME (TME$_n$) and true amino acid availability were determined. In experiment 2, 324 day-old chicks were used in a 2${\times}$3 factorial design consisting of two energy contents (high and low) and three GAL levels (0, 250, and 500 mg/kg). Three feeding phases, comprising 0-21 d, 22-35 d and 36-48 d, were involved. GAL addition improved TME$_n$ and the availability of methionine and cystine (p<0.05). The apparent ME (AME) or nitrogen-corrected AME (AME$_n$) and digestibility of dry matter, organic matter, calcium, and phosphorus were improved significantly on d 21, so was crude protein and an interaction of energy and GAL on AME$_n$ (p<0.05) was found on d 35. However, daily intake and daily gain were significantly improved with GAL addition (p<0.05) during 21 d. The small intestine relative weight decreased at 250 mg/kg GAL (p<0.05) on d 35, whereas presented an interaction between GAL and energy on d 21 (p<0.05). Likewise, this treatment increased breast muscle ratio (p<0.05). On d 21, triglycerides level of broilers showed interaction between energy and enzyme levels (p<0.05). Uric acid level in 500 mg/kg GAL declined linearly (p<0.05). On d 35, quadratic effects (p<0.05) were observed in total protein, albumin, globulin and cholesterol content for enzyme supplementation. And the interactive effects of energy and GAL on serum values showed more obviously. The study implies that GAL improved energy and nutrient availability of corn-soybean meal diet in broiler. The GAL supplementation to corn-soybean meal based diet can improve performance of broilers in early stages of growth.