• The Korean Journal of Zoology
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• v.14 no.4
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• pp.181-191
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• 1971

In Diplonichus esakii each ovary comprises five telotrophic ovarioles. In the fourth instar, the anterior part of the germarium consists of undifferentiated cells. The middle part contains a spherical trophic and the posterior part comprises young oocytes, followed by the upside downbell shape prefollicular tissue. The bell form is the standard characteristic in this instar larva, and the nutritive cord is found although somewhat indistinctly. In the fifth instar larva, the trophic core is elliptical form, and the oocyte is found at the base of the core. The oocytes are connected with the ocre by the nutritive cord. In the prefollicular tissue are also found some oocytes. In the adult, the vitellarium is filled by the developmental oocytes. The yolk granules inside each oocyte migrate from the base of the follicular epithelial cells to the center of the oocyte. Finally, the ooplasm of the oocyte becomes completely homogeneous. Therefore, according to the advancing of instars the nutritive cord developes completely before the oocyte has chorion and the follicular epithelial cell binucleates. The upper part of the ovariole consists of unchorionated oocytes, and the proximal part comprises chorionated oocytes in the adult.

• Journal of Microbiology
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• v.34 no.3
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• pp.241-247
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• 1996

The stability of the genetically engineered microorganisms and their recombinant plasmids released in natural environments has been regarded as one of the molecular ecological topics. In this study, the recombinant plasmids pCU103 in which the pcbCD genes involved in biodegradation of biphenyl and 4-chlorobiphenyl were cloned in pBluescript SK(+) vector, were examined for their structural and functional stability in different waters at 15 $^{\circ}C$ by the methods of electrophoresis, Southern hybridization, quantification with fluorescent dye, and transformation. The recombinant plamids maintained their stabilities for about 30 days in sterilized distilled water (SDW), 15 days in autoclaved creek water (AW), 25 days in filtered and autoclaved non-sterile creek water (FAW), 4 days in Luria-Bertani (LB) broth, and less than one day in filtered non-sterile creek water (FW). The covalently closed circular (CCC) form of the plasmid was decreased and open circular (OC) form was increased as a function of incubation time, and then linear (L) form was produced to be ultimately degraded out. The degradation rates of the plasmid were proportionally correlated to trophic level of the water, and the biological factor such as DNases was found to be one of the most critical factors affecting structural and functional stability of the plasmid in non-sterile natural water.

• Advances in environmental research
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• v.1 no.1
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• pp.15-35
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• 2012

Mercury (Hg) is a global environmental pollutant that has been the cause of many public concerns. One particular concern about Hg in aquatic systems is its trophic transfer and biomagnification in food chains. For example, the Hg concentration increases with the increase of food chain level. Fish at the top of food chain can accumulate high concentrations of Hg (especially the toxic form, methylmercury, MeHg), which is then transferred to humans through seafood consumption. Various biological and physiochemical conditions can significantly affect the bioaccumulation of Hg-including both its inorganic (Hg(II)) and organic (MeHg) forms-in fish. There have been numerous measurements of Hg concentrations in marine and freshwater fish worldwide. Many of these studies have attempted to identify the processes leading to variations of Hg concentrations in fish species from different habitats. The development of a biokinetic model over the past decade has helped improve our understanding of the mechanisms underlying the bioaccumulation processes of Hg in aquatic animals. In this review, I will discuss how the biokinetic modeling approach can be used to reveal the interesting biodynamics of Hg in fish, such as the trophic transfer and exposure route of Hg(II) and MeHg, as well as growth enrichment (the increases in Hg concentration with fish size) and biomass dilution (the decreases in Hg concentration with increasing phytoplankton biomass). I will also discuss the relevance of studying the subcellular fates of Hg to predict the Hg bioaccessibility and detoxification in fish. Future challenges will be to understand the inter- and intra-species differences in Hg accumulation and the management/mitigation of Hg pollution in both marine and freshwater fish based on our knowledge of Hg biodynamics.

• Parasites, Hosts and Diseases
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• v.32 no.3
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• pp.171-176
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• 1994

Wistar rats were induced of Pneumowstis cayinii infection by injection with methyl-prednisolone to correlate the cyst counts and numbers of nuclei. Seven sections of the lungs were examined by impression smears and also whole lung homogenates were screened for nucleus counting for each rat. At the first week of the experiment, all of the Impression smears except one were cyst negative but trophic forms were counted around 106. At the third week, the cysts appeared one per 20 Immersion oil lens fields. The nuclei were on the order of 107 at this period, and this amount of Pc is regarded as the limit of cyst detection on Impression smears. When the nuclei were over 109 in the lungs, the cysts were counted about 50 in 20 microscopic fields. The organisms were distributed in the lungs without any predilection focus. The present data suggest that the trophic forms, proliferate first and the cysts appear later in the lungs.

• Journal of Environmental Impact Assessment
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• v.21 no.3
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• pp.461-468
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• 2012

In general, long-term changes of ecological factors take a pulse form in which they interact with other factors and go through a repeated increasing and decreasing cycle. The coupling of the two approaches the grid model and the box model in ecological modeling can lead to an in-depth understanding of the environment. The study analyzes temporal variations of major storages with an energy system model that formulizes effectively the relationships among nutrients, phytoplankton, and zooplankton in the Yellow Sea and the East China Sea. An increase of light intensity and standing stock of nutrient increase the magnitude and frequency of pulsing. Also, an immense reduction of nutrient concentration can cause extinction of the pulsing and bring about a steady state. It is concluded that the nutrient loads in freshwater discharge from the Yangtze affect the cycles of major ecological components as well as water quality variables and play an important role in the marine ecosystem.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.380-389
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• 2007

Stable carbon and nitrogen isotopes were analyzed in suspended particulate organic matter, macroalgae and macrobenthic invertebrates in order to determine the importance of primary organic matter sources in supporting food webs of rocky subtidal and intertidal macroalgal beds in the Korean coasts. Investigations were conducted at the inter tidal sites within Gwangyang bay, a semi-enclosed and eutrophicated bay, and the subtidal sites of the east coast, a relatively oligotrophic and open environment, in May and June 2005. Water-column suspension feeders showed more negative $\delta^{13}C$ values than those of the other feeding guilds, indicating trophic linkage with phytoplankton and thereby association with pelagic food chains. In contrast, animals of the other feeding guilds, including interface suspension feeders, herbivores, deposit feeders, omnivores and predators, displayed relatively less negative $\delta^{13}C$ values than those of the water-column suspension feeders and similar with that of macroalgae, indicating exclusive use of macroalgae-derived organic matter and association with benthic food chains. Most the macrobenthic species were considered to form strong trophic links with benthic food chains. In addition, the distribution of higher $\delta^{15}N$ values in macrobenthic consumers and macroalgae at the intertidal sites of Gwangyang Bay than those at the subtidal sites of the east coast suggests that anthropogenic nutrients may enhance the macroalgal production at the intertidal sites and in turn be incorporated into the particular littoral food web in Gwangyag Bay. These results confirm the dominant role of macroalgae in supporting rocky subtidal and intertidal food webs in the Korean coasts.

• Ocean and Polar Research
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• v.33 no.spc3
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• pp.349-358
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• 2011

In order to ascertain latitudinal variation of lipid contents and compositions in copepods, we collected warm water copepod species (Euchaeta sp. and Pleuromamma spp.) from four different regions from low (sub-tropical) to mid (temperate) latitudes in the Northwest Pacific Ocean. Total lipid contents of Pleuromamma spp. were about 11 $ug{\cdot}ind^{-1}$ with little latitudinal variation, whereas Euchaeta sp. showed slightly higher lipid content (20 $ug{\cdot}ind^{-1}$) than Pleuromamma spp. with latitudinal gradient (low at subtropic and high at temperate). Wax esters, known as the major storage lipid classes, were found to be the dominant lipid classes (accounting for more than 35% of total lipids) in Euchaeta sp., whereas in Pleuromamma spp., phospholipids, known as cellular membrane components, were the dominant lipid classes. However, the exception was specimens from warm pool region exhibiting dominance in storage of lipids as a form of triacylglycerols. Among fatty acids, polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (DHA : 22:6(n-3)) (about 35% of total fatty acids), were most abundant in Euchaeta sp., while saturated fatty acids (SAFA), specially hexadecanoic acid (16:0) (about 30% of total fatty acids), were most abundant in Pleuromamma spp.. Among the neutral fraction of lipids, phytol, originated from the side chain of chlorophyll, was found in all samples which generally indicate active copepods feeding on algae. While only trace amounts of short-chain fatty alcohols were found in Pleuromamma spp., significant amounts of fatty alcohols were found in Euchaeta sp.. Particularly, significant amounts of long chain monounsaturated fatty alcohols (20:1 and 22:1), generally found in cold water species, were found in Euchaeta sp. from low latitudes. The latitudinal variation of trophic lipid markers in these copepods could be significantly related with in-situ food availability and species-specific diet preference. The result of this study suggests that the lipid contents and compositions in copepods may not only indicate their nutritional condition and feeding ecology but also provide insight into species-specific living strategies under different environmental conditions (i.e. water temperature, food availability).

• Journal of Korean Society of Disaster and Security
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• v.13 no.2
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• pp.65-75
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• 2020

The efficiency of sewage treatment was ananlyzed selecting a sewage treatment plant in Gyeonggi-do where A₂O process was applied. Statistical techniques based on the operation data of the sewage treatment were used. The main factors directly affecting the efficiency of the treatment process were analyzed using a GPS-X model. The correlation analysis and one-way ANOVA were performed. The T-N and NH₄⁺-N values of the effluent did not generate statistically significant level (p-value:>0.05) when compared with C/N ration values. Removel of nitrogen components form sewage treatment plants were affected by temperature, HRT, SRT and DO. In the case of BOD, all operating factors were affected, while COD was affecte by factors of HRT, STR and DO. In simulations using GPS-X, the parameters that greatly influence was included the maximum sedimentation rate, the dependent nutrient microbial yield (anoxic), the phosphorus saturation coefficient, the dependent nutrient microbial killing rate, the dependent nutrient microbial maximum growth rate, and the independent trophic microorganisms. The maximum growth rate and the maximum setting rate were identified.