Parasites, Hosts and Diseases
- Volume 32 Issue 3
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- Pages.171-176
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- 1994
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Relation of cyst counts with numbers of total nuclei of Pneumocystis carinii in rats
흰쥐 주폐포자충에 있어서 도말표본상 포낭의 수와 핵의 총 수와의 상관성
- Hong, Seong-Tae (Department of Parasitology and Institute of Endemic Deiseases Seoul National University, College of Medicine) ;
- Yu, Ji-Suk (Department of Parasitology and Institute of Endemic Deiseases Seoul National University, College of Medicine) ;
- Lee, Mi-Jeong (Department of Parasitology and Institute of Endemic Deiseases Seoul National University, College of Medicine)
- 홍성태 (서울대학교 의과대학 기생충학교실 및 풍토병연구소) ;
- 유지숙 (서울대학교 의과대학 기생충학교실 및 풍토병연구소) ;
- 이미정 (서울대학교 의과대학 기생충학교실 및 풍토병연구소)
- Published : 1994.09.01
Abstract
Wistar rats were induced of Pneumowstis cayinii infection by injection with methyl-prednisolone to correlate the cyst counts and numbers of nuclei. Seven sections of the lungs were examined by impression smears and also whole lung homogenates were screened for nucleus counting for each rat. At the first week of the experiment, all of the Impression smears except one were cyst negative but trophic forms were counted around 106. At the third week, the cysts appeared one per 20 Immersion oil lens fields. The nuclei were on the order of 107 at this period, and this amount of Pc is regarded as the limit of cyst detection on Impression smears. When the nuclei were over 109 in the lungs, the cysts were counted about 50 in 20 microscopic fields. The organisms were distributed in the lungs without any predilection focus. The present data suggest that the trophic forms, proliferate first and the cysts appear later in the lungs.
Wistar 계통 휜쥐를 면역억제하여 주폐포자충을 발현시킨 후, 기간 별로 폐단면 도말검사 표본에서 포낭의 수를 세고 폐 전체를 균질액으로 만들어 주폐포자충의 핵을 세었다. 또한 폐의 7개 부위에 따라서 포낭의 수를 비교하였다 포낭은 실험 3주가 되어서야 도말표본에서 20개의 1000배 확대 시야에서 평균 1개 이상이 검출될 정도로 나타났으나 폐 균질액에서 주폐포자충의 영양형은 실험 1주 후부터