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Identification and Characterization of Paraconiothyrium brasiliense from Garden Plant Pachysandra terminalis (가든식물 수호초(Pachysandra terminalis)로부터 Paraconiothyrium brasiliense의 분리 및 동정)

  • Choi, Min Ah;Park, Seung Jun;Ahn, Geum Ran;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.262-268
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    • 2014
  • A fungal isolate DUCC5000 from a garden plant Pachysandra terminalis was identified as Paraconiothyrium brasiliense based on the results of morphological and molecular studies. The fungus formed brown to black conidiomata of (0.2-0.7)-2(-3.5) mm singly or as a group on PDA. Conidia measured $2-5{\times}1.8-3{\mu}m$ in size, hyaline, ellipsoid to short-cylindrical, and rounded at both ends. The internal transcribed spacer (ITS) DNA of the isolate shared 100% nucleotide sequence homology with those of known P. brasiliense isolates. Phylogenetic tree inferred from the ITS sequence analysis showed that the DUCC5000 isolate formed a clade with known isolates of P. brasiliense. The fungal mycelia grew better on oatmeal agar than on MEA and PDA. On PDA media under various pH conditions, fungal mycelial growth was observed at pH 9. Colony morphology of the fungus tended to alter depending on the kinds of nutrient media and pH condition. On chromagenic media, the fungus demonstrated its ability to produce extracellular enzymes including amyalse, avicelase, ${\beta}$-glucosidase, protease, and xylanase. However, in pathogenicity testing, no disease symptoms were observed on the leaves of P. terminalis. This strain is the first report on P. terminalis in Korea.

The State of Mulberry Cultivation and It's Development in High Land of Benguet Province, Philippines (필리핀 벤규트의 고지대 뽕밭현황 및 개선방안)

  • Ryu, Keun Sup
    • Current Research on Agriculture and Life Sciences
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    • v.14
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    • pp.85-100
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    • 1996
  • The Studies were conducted to provide the state of mulberry cultivation and it's development in Banguet province (high land) of Philippines. Philippines initiated the sericulture industry with the technical asistance of Japan in 1974 and established mulberry field and sericultural facilities with technical asistance of Korea in 1990 and 1995. The required average of 100 to 150mm per month is not available during the dry season from December to March. Therefore mulching with grass which is available abundantly in the Philippines should be established to conserve moisture, to control soil erosion, surface ran-off and also to increase the humus content in the soil. In chemical properties of mulberry field, the pH value of soil is 4.7, organic matter 1.6%, and available phosphorus 6ppm. Therefore, all fields should do liming and be applied compost. To improve leaf yield for mulberry planted under partial shade area of pine trees, more pruning of pine tree should be done for good sunshining of mulberry, more liming and compost should be applied to improve acidic soil. To control the leaf roller, DDVP and KAFIL are able to be used. When spraying insecticides to control mulberry insect pests, care should be taken to consider the residual effects of chemicals on the leaf. Leaf should be fed to silkworms only after the leaves are free of any residual effects.

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Biomass and Net Primary Production of Quercus variabilis Natural Forest Ecosystems in Gongju, Pohang, and Yangyang Areas (공주(公州), 포항(浦港), 그리고 양양(襄陽) 지역(地域) 굴참나무 천연림(天然林) 생태계(生態系)의 물질생산(物質生産)에 관(關)한 연구(硏究))

  • Park, Gwan-Soo;Lee, Sung-Woo
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.692-698
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    • 2001
  • This study has been carried out to estimate aboveground biomass and net primary production(NPP) in an average 41-years-old Quercus variabilis stand of Gongju area, 45-years-old Quercus variabilis stand of Pohang area, and 54-years-old Quercus variabilis stand of Yangyang area. Ten sample trees were cut in each forest and soil samples were collected in July to August, 2000. Estimation for aboveground biomass and net primary production were made by the equation model $Wt=aD^b$ where Wt is oven dry weight in kg and D is DBH in cm. Total aboveground biomass was 91.31ton/ha in Gongju area, 207.6ton/ha in Pohang area, and 71.39ton/ha in Yangyang area. The aboveground biomass 207.6ton/ha in Pohang area is the highest biomass production among the amount of biomass in Quercus variabils stands reported in Korea. The proportion of each tree component to total aboveground biomass was high in order of bolewood, bolebark, branches and leaves in the three forests. Aboveground total net primary production was estimated at 7.8ton/ha in Gongju area, 11.5ton/ha in Pohang area, and 6.40ton/ha in Yangyang area. There were at least 2 times higher total aboveground biomass in Pohang area than in the Gongju and Yangyang areas because of climate difference among the study areas.

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Appropriateness Evaluation of Plug Seedling Cultivation for Replanting of Rehmannia glutinosa (Gaertn.) Libosch. ex Steud (보식용 지황의 플러그 육묘 재배를 위한 적정성 평가)

  • Lee, Sang Hoon;Kang, Seung Uk;Lee, So Hee;Koo, Sung Cheol;Hur, Mok;Jin, Mei Lan;Lee, Woo Moon;Park, Min Su;Kim, Yeon Bok;Han, Jong Won
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.3
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    • pp.202-207
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    • 2019
  • Background: Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae and is used in traditional oriental medicine. This study was conducted to evaluate the appropriateness of plug-seedling cultivation for replanting of R. glutinosa. Methods and Results: The seed rhizomes of Rehmannia glutinosa 'Jihwang 1' were harvested in the middle of March, 2018. Direct seedling cultivation was conducted after air curing for 3 days. The seedlings for transplanting were cultivated for 30 (30D), 45 (45D), and 60 days (60D). The mat formation rates of the 30D, 45D and 60D treatment seedling were 81.3%, 100%, and 100%, respectively. Of the tree treatment groups, flowering occurred only in the 45D and 60D treatment groups, and their flowering rates were $7.3{\pm}3.06%$ and $13.3{\pm}1.15%$, respectively. The agronomic characteristics and active components of the three treatment groups were evaluated in comparison with the DS1 (directly seeded group, control) and DS2 (directly seeded group, replanting) groups. As a result, the leaf length and leaf width of the 30D seedlings were higher than other treatment groups, but the number of leaves per plant was statistically the same. In addition, root diameter and yield of the 30D seedling were statistically the same as DS1. Moreover the catalpol contents of 30D, 45D, DS1 and DS2 group seedlings were 2.23%, 1.86%, 2.50% and 2.08%, respectively. Conclusions: Taken together, these findings suggest it is feasible to use the plug-seedling cultivation method for replanting of R. glutinosa. seedlings. However, further evaluation of the economic aspect is needed.

Changes of Photosynthesis, Leaf and Fruit Characteristics of Actinidia arguta and Hybrid Kiwi (A. arguta × A. deliciosa) According to Crown Layer (다래(Actinidia arguta) 및 교잡종 다래(A. arguta × A. deliciosa)의 수관층위에 따른 광합성, 엽 그리고 과실의 특성 변화)

  • Park, Youngki;Han, Jingyu;Hwang, Suk-In;Kim, Sea-Hyun;Kang, Moon-Su
    • Journal of Korean Society of Forest Science
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    • v.100 no.1
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    • pp.8-13
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    • 2011
  • This study was carried out to investigate the change of photosynthesis, leaf and fruit according to crown layers in Actinidia arguta and hybrid kiwi. Photosynthesis rate (PPFD $1,000{\mu}mol{\cdot}m^{-2}s^{-1}$) were 5.82, 7.11, $9.54{\mu}mol\;CO_2\;m^{-2}s^{-1}$ at lower, middle, and upper position, respectively in 3 layer cultivation type. The length and area of leaves collected from lower position have lager value than upper position. The fruit quality of A. arguta and hybrid kiwifruit increased with increasing crown height. A linear correlation ($R^2=0.72$, 0.89 and 0.98) was shown between fruit weight (Boeun 4, Injea 16 and HM3) and the height of crown layer(layer 1, 2, and 3). From the results, we can suppose that the tree of the upper position of crown layer in the cultivation of A. arguta and hybrid kiwifruit produce high quality fruit due to its high photosynthesis ability.

A Study on Germination Characteristics through Seed Variable Immersion for Restoration of North Korea Forest (북한산림복구용 종자 변온침지 처리를 통한 발아특성 연구)

  • Choi, Jong-O;Park, Yong-Jin
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.1
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    • pp.1-10
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    • 2022
  • This study was conducted to investigate the germination characteristics through the temperature change treatment compared to the general room temperature immersion treatment of the native species, the Larix kaempferi, and the Berula platyphyllavar. japonica seeds, and to obtain basic data for the production of healthy seedlings with high productivity and efficiency. As a result, the germination rate of the larch seeds showed a significant difference according to room temperature and temperature immersion treatment, and the average germination days of the birch seeds showed a significant difference according to room temperature immersion and temperature immersion treatment. On the other hand, there was no significant difference in germinal uniformity. The results of the study are as follows. First, the results of the analysis of the fallen leaves were the highest germination rate in the treatment of 1.5 days of hot water and 1.5 days of cold water, the fastest germination rate, the lowest average germination day, and the highest germination uniformity. This is the best result in most items, and the change temperature immersion treatment of hot and cold baths was superior to the conventional room temperature immersion treatment. Second, the results of birch tree showed that the germination rate was the highest in 1.5 days of hot water and 1.5 days of cold water. The average germination day is 1.5 days of cold water 1.5 days of hot water 1.5 days, but the difference between 1.5 days of hot water and 1.5 days of cold water is 0.01 days. The highest germinated uniformity was found in 1.5 days of cold water and 1.5 days of hot water. As a result, in the case of larch and birch, the seed temperature immersion (1.5 days of hot water, 1.5 days of cold water) treatment is superior to the existing three-day immersion treatment in various analysis methods, and it is expected that productivity and efficiency can be improved at a low cost in a short period of time at the seedling production site through seeds.

Effects of Salix subfragilis communities on water quality in Namgang Dam reservoir (남강댐 선버들 군락이 수질에 미치는 영향)

  • Kim, Ki Heung
    • Journal of Korea Water Resources Association
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    • v.55 no.12
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    • pp.1065-1076
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    • 2022
  • The purpose of this study was to investigate the effect of the expansion and withering of Salix subfragilis communities on the water quality in Namgang Dam reservoir. The distribution area of the Salix subfragilis communities was 0.12 km2 in 2003 for the first time, but it was 3.58 km2 in 2019, which has increased rapidly by about 30 times in 16 years. However, in 2013, the distribution area has decreased by 0.17 km2 due to long-term immersion in high turbidity, and self-thinning in Salix subfragilis communities. The lake characteristics of reservoir showed a combination of lake type and river type in terms of average water depth, watershed area/lake surface area ratio, water residence time, flushing rate, and stratification. From the result of analyzing long-term changes in lake water quality, COD, TP, and chlorophyll-a in Salix subfragilis communities were significantly larger than those in the three points located in the central part of reservoir. In particular, the fact that the value of chlorophyll-a showed the maximum value in winter rather than summer, unlike the trend of the three points in the Namgang Dam water quality monitoring network, is thought to have occurred internally rather than externally. It can be estimated that one cause of this deterioration of the water quality in Namgang Dam reservoir is the huge amount of nutrients generated in the decomposition process of by-products such as fallen leaves, branches and withered trees in Salix subfragilis communities.

Soil and Leaf Chemical Properties and Fruit Quality in Kiwifruit Orchard (국내 키위 주산지 토양 및 엽 화학성과 과실 특성)

  • Kim, Hong Lim;Lee, Mock-hee;Chung, Kyeong-Ho
    • Korean Journal of Environmental Agriculture
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    • v.41 no.3
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    • pp.158-166
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    • 2022
  • BACKGROUND: Kiwifruit is a fruit tree with relatively small cultivation area in Korea and researches on its soil and physiology are very limited compared to those on cultivar development. Therefore, there are limited information for farmers to cope with the reduction in productivity due to various physiological disorders and premature aging. This study was conducted to investigate the soil and leaf chemical properties, and fruit characteristics, which will be used as basic data for stable kiwifruit orchard soil management. METHODS AND RESULTS: The soil and leaf chemical properties, and fruit characteristics were investigated for two years in 16 kiwifruit orchards growing 'Hayward' (Actinidia deliciosa) in Jeollanam-do and Gyeongsangnam-do. Soil and leaf samples were collected in July and fruit quality was investigated by harvesting fruits about 170 days after full bloom. The average soil chemical properties of kiwi orchards were generally higher than the recommended level, except for pH, and especially, the exchangeable potassium reached about 300% of the recommended level. The proportions of orchards that exceeded the recommended level of soil chemical properties were 63, 31, 100, 69, 94, 88 and 69% for pH, EC, organic content, available phosphate, and exchangeable potassium, calcium and magnesium, respectively. Thirty-three percent of orchards had more than 100 mg/kg of nitrate nitrogen in soil. Available phosphate in soil showed a significantly positive correlation with leaf nitrogen, phosphoric acid and calcium content, but showed a significantly negative correlation with leaf potassium content. The magnesium content in the leaves was significantly correlated with soil pH. The highest fruit weight was observed in about 25 g/kg of leaf nitrogen content which could be attained when plants were grown on the soil containing about 100 mg/kg of nitrate nitrogen content. The average soluble solids content among 16 orchards was 9.58 °Brix at harvest and 13.9 °Brix after ripening, which increased about 45%, and the average fruit weight was about 110 g. CONCLUSION(S): For fruit quality, fruit soluble solids (sugar compounds) content was significantly correlated with leaf potassium content, fruit hardiness with leaf total nitrate, calcium and magnesium, and fruit titratable acidity with leaf magnesium; however, leaf calcium and magnesium negatively affect the soluble solids contents in fruits.

First Report of Fire Blight Caused by Erwinia amylovora on Korean Mountain Ash (Sorbus alnifolia) in Korea (Erwinia amylovora에 의한 팥배나무 화상병 발생 보고)

  • Yeon-Jeong Lim;Hyeonseok Oh;Mi-Hyun Lee;Eunjung Roh;Hyeonheui Ham;Dong Suk Park;Duck Hwan Park;Yong Hwan Lee
    • Research in Plant Disease
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    • v.29 no.1
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    • pp.79-81
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    • 2023
  • During the nationwide survey of fire blight, the typical shoot blight symptoms were found on Korean mountain ash (Sorbus alnifolia) which was located near an orchard that produced fire blight on pear trees in Eumseong, Korea, May 2021. To identify the causal agent, we progressed isolation from the symptomatic leaves and shoots. Two white and mucoid colonies were isolated into the pure culture. Two isolates were identified as Erwinia amylovora according to the colony-polymerase chain reaction (PCR) with amsB primers and the phylogenetic tree using 16S rRNA sequences. To test of pathogenicity of two isolates, we inoculated immature pear fruits and understock of apple. We observed necrosis and oozes on immature pear fruits and shoot blight resulting in necrosis on apple shoots six days after inoculation. Colonies were recovered from the inoculated pears and apples, and identity was confirmed through colony PCR for amsB genes. To our knowledge, E. amylovora was first reported on Korean mountain ash native to South Korea.

Sprout Growth and Its Effects on Vegetation after Lumbering of Populus albaglandulosa (은수원사시나무 벌목 후 맹아의 생장과 맹아가 주변 식생에 미치는 효과)

  • Min, Byeong-Mee;Choi, Sun-Hee
    • The Korean Journal of Ecology
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    • v.23 no.3
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    • pp.223-229
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    • 2000
  • To develop the restoration technique from urban planted forest to natural forest, sprout growth and its effects on vegetation composition in the early stage after lumbering, were studied, using only Populus albaglandulosa of the tree layer in Chungdam Park Chungdam-dong, Kangnam-gu, Seoul during the two years from 1997 to 1998. The results were as follow. After lumbering, the more basal areas of the parent stump, the more sprouts were generated. The basal area of parent stump was related more to biomass than to the number of sprouts. In the cutting area, biomasses of sprouts were 552.7 g?DW/㎡ in first year and 8.7 g?DW/㎡ in second year after lumbering. Therefore, cutting of sprouts in first year resulted in decrease of sprout production in second year. In the non-cutting area, biomass of sprouts was 657.4 g?DW/㎡ and more than that of the experiment. Especially, 2-year sprouts grew to a 304 cm height by the end of the growing season in the second year. The growing season of sprouts was from early April to early September and almost all the leaves of the sprouts remained at the end of the growing season. Specific leaf area decreased along with time elapse and plant height but the differences were not conspicuous. Therefore, it is thought that the reason why p. albaglandulosa sprouts grew fast was due to a long growing period and lower layer leaves which remained at the end of the growing season. In the cutting area of the P. albaglandulosa sprouts, there were no changes in the number of species in the shrub layer but an conspicuous increase in the herb layer. Coverage was no changes in the shrub layer but an increase in the herb layer. In the non-cutting area of P albaglandulosa sprouts, the number of species and the coverage changed a little, and the main species of the herb layer were Phytolacca americana, Eupatorium rugosum and Panicum dichotomiflorum, which are tall plant species.

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