Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Fire Blight Caused by Erwinia amylovora on Korean Mountain Ash (Sorbus alnifolia) in Korea

Erwinia amylovora에 의한 팥배나무 화상병 발생 보고

  • Yeon-Jeong Lim (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hyeonseok Oh (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Mi-Hyun Lee (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Eunjung Roh (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hyeonheui Ham (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Dong Suk Park (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Duck Hwan Park (Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Yong Hwan Lee (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration)
  • 임연정 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 오현석 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이미현 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 노은정 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 함현희 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 박동석 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 박덕환 (강원대학교 스마트농업융합학과) ;
  • 이용환 (농촌진흥청 국립농업과학원 작물보호과)
  • Received : 2022.10.05
  • Accepted : 2022.12.20
  • Published : 2023.03.31
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Abstract

During the nationwide survey of fire blight, the typical shoot blight symptoms were found on Korean mountain ash (Sorbus alnifolia) which was located near an orchard that produced fire blight on pear trees in Eumseong, Korea, May 2021. To identify the causal agent, we progressed isolation from the symptomatic leaves and shoots. Two white and mucoid colonies were isolated into the pure culture. Two isolates were identified as Erwinia amylovora according to the colony-polymerase chain reaction (PCR) with amsB primers and the phylogenetic tree using 16S rRNA sequences. To test of pathogenicity of two isolates, we inoculated immature pear fruits and understock of apple. We observed necrosis and oozes on immature pear fruits and shoot blight resulting in necrosis on apple shoots six days after inoculation. Colonies were recovered from the inoculated pears and apples, and identity was confirmed through colony PCR for amsB genes. To our knowledge, E. amylovora was first reported on Korean mountain ash native to South Korea.

2021년 5월 화상병 확산 방지를 위해 화상병 예찰 중에 충북 음성의 화상병이발병한 배 과수원 바로 인근의 밭에서 자라는 팥배나무의 어린 가지가 수침상을 보이면서 마르는 증상을 발견하였다. 채집한 잎과 어린 가지로부터 균 분리를 진행한 결과 화상병원균과 유사한 유백색의 mucoid한 colony를 보이는 2개 균주를 순수 분리하였다. 순수 분리된 병원균을 화상병원균 특이 프라이머 PCR 검정 및 16S rRNA 염기서열을 이용한 계통도 분석을 통해 분리된 균주가 Erwinia amylovora임을 확인하였다. 배 유과와 사과 대목에 병원성 검정을 수행한 결과 접종 6일 후에 접종한 자리에 괴사 증상과 우즈를 확인할 수 있었고, 이들 증상에서 동일한 균을 재분리하였다. 본 연구는 국내에서 최초로 E. amylovora에 의해 발생한 팥배나무에서의 화상병을 보고하고자 한다.

Keywords

Acknowledgement

This study was carried out with the support of Cooperative Research Programs (Project No. PJ015059) from Rural Development Administration, Republic of Korea.

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