• 한국임상수의학회지
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• 제34권4호
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• pp.304-306
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• 2017

A 3-year-old Thoroughbred mare was referred to J&C Equine Hospital with gestation day 30 twin pregnancy. On transrectal ultrasonographic examination, two similar sized (28 mm) embryonic vesicles, unilaterally fixed in the uterine horn, were detected. Transvaginal ultrasound-guided aspiration (TUGA) was performed for reduction of one embryonic vesicle. Yolk sac and allantoic cavity fluids from one embryonic vesicle were aspirated by a needle guided by using transvaginal ultrasound. The mare continued normal singleton pregnancy after twin reduction and delivered a foal successfully. This is the first case described the clinical use of TUGA in Korea. Clinical use of TUGA in twin reduction after embryonic fixation is recommended for equine clinicians.

• 대한영상의학회지
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• 제84권6호
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• pp.1233-1243
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• 2023

경피적 초음파 유도생검은 진단 및 치료 계획을 정하기 위해 다양한 신체 병변에 대한 병리 확인에 유용한 시술이다. 하지만 깊은 골반 병변은 병리진단이 어려운데 장, 방광, 주요 혈관, 그리고 골반뼈로 인해 경피적 접근이 어렵고 위험하기 때문이다. 여성의 경우 질은 탄력적이고 골반의 내부 장기와 가깝다. 따라서 경질 초음파는 골반 병변에 대한 병리진단을 위해 효과적이고 안전한 길잡이 역할을 할 수 있다. 이 종설의 목적은 경질 초음파 유도생검의 적응증과 방법을 소개하고, 또한 보고된 진단 정확도 및 안정성을 기술하고자 한다.

• 한국수정란이식학회지
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• 제16권2호
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• pp.145-152
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• 2001

• 한국수정란이식학회지
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• 제13권1호
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• pp.77-86
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• 1998

A combined technology of transvaginal ovum pick-up(OPU) system with in vitro-oocyte manipulation technique can be used for improving reproductive efficiency in the cattle. The objective of this study was to establish a newly-conceived breeding program using OPU in the pregnant cows. The OPU trial was performed in pregnant cows every 10 days from 40 through 90 days of artificial insemination (Al), and number of follicles in ovary, number of retrieved oocytes and embryo development following in vitro-fertilization, were evaluated. Reduced number of follicles in the ovaries of pregnant cows was firstly detected from 70 days after A' and a significant (P<0.05) decrease in the follicle number (5.4 follicles /donor) was found at 90 days than at 40, 50, 60 and 80 days after Al (8.0~9.2). A similar pattern was also observed in the number of oocytes retrieved by OPU apparatus during experimental period. When retrieved oocytes were matured and inseminated in vitro with frozen bull semen, development of the oocytes to the blastocyst stage was not significantly affected by the retrieval time. Four embryos (morula or blastocyst stage) derived from oocytes retrieved from pregnant cows were nonsurgically transferred to four recipient cows on day 7 of estrus cycle. For the first time in Korea, three of four transferred embryos developed to live calves with normal physiological parameters. In conclusion, an effective breeding program employing pregnant cow can be developed by use of OPU trial and in vitro culture techniques of oocytes ; OPU system could be repeated in pregnant cows with no risk of abortion and viable offsprings were borne after transfer to the recipients.

• 한국수정란이식학회지
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• 제12권2호
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• pp.203-209
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• 1997

This study was carried out to compare the actual size(length and height) of ovaries, follicles and corpora lutea of Korean native cow with those on sonograms. We used 3 different probes(3.5 MHz abdominal probe, 6.5 MHz transvaginal probe and 5.0 MHz transrectal probe) and a calipher for measurements of ovaries, follicles and corpora lutea on sonograms and actual size. Under water immersion, 157 ovaries were scanned with 3 probes and measured in actual size and compared each other. The average height and width of ovaries of Korean native cows were 17.40$\pm$3.99 and 34.23$\pm$6.02mm, respectively. In comparison of height, length of ovaries and preovulation follicles, we found that image with a transvaginal probe was nearly the same as the actual size(p<0.01), but with an abdominal probe the image was appeared larger than the actual size. In measurement(diameter) of preovulation follicles the transvaginal probe was proven to be more accurate to the actual size than other probes and in corpus luteum measurement all probes were accurate. In the comparison of number of follicles by different size ranges, there was no statistical difference in the count of follicles over 10 mm in diameter between the transvaginal probe and naked eyes.

• 한국가축번식학회지
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• 제24권1호
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• pp.77-82
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• 2000

한우에 있어서 생체내 난자 채취기술과 체외수정 기술을 접목하여 고능력 한우로부터 수정란을 대량 생산하기 위한 기초 연구로서, 발정주기에 따른 난소의 크기와 난포의 발육 양상을 초음파진단기를 이용하여 조사하였으며, 여러 마취.진정제를 조합 이용하여 마취효과를 조사하여 얻어진 결과는 다음과 같다. 1. 한우의 난소의 크기는 도축 난소의 실측치와 생체에서 초음파진단기를 이용한 측정치간에 유의적인 차이가 인정되지 않았으며, 발정주기중 난포기때와 황체기때 크기를 비교해도 유의적인 차는 인정되지 않았다. 2. 한우의 발정주기중 난소내 발육하는 소난포와 중난포의 수는 3일과 12일 째에 증가하는 한 발정주기에 2회의 난포발육기가 있었다. 3. 한우의 최적 마취조합은 0.3$m\ell$ 의 Rompun(equation omitted)을 근육주사하고 5$m\ell$의 lidocaine 을 경막외 마취한 후 lidocaine 2$m\ell$을 자궁경에 도포하는 경우였다.

• 한국수정란이식학회지
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• 제11권2호
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• pp.103-109
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• 1996

The aim of this study was to evaluate the effect of recombinant bovine somatotropin (bST; Boostin-S, LG Chem) treatment with FSH (Super OV) or PMSG on superovulatory response for transvaginal ultrasound-guided oocyte retrieval (TVR) in calves. Eight Korean Native Cattle(KNC) heifer calves; 150 to 240 days old; were randomly assigned to four treatment groups: 1) FSH(75 mg); 2) FSH (75 mg) + bST(500mg) 3) PMSG(1;000 IU); 4) PMSG(1, 000 IU) + bST(500 mg). Experimental calves in group 1 (n=2) and 2(n=2) were weekly superovulated for 4 consecutive weeks with daily injection of FSH for 3days and the next day subjected to TVR session. Animals in group 3 (n=2) and 4(n=2) were weekly stimulated for 4 consecutive weeks with a single dose of 1, 000 IU PMSG. TVR was performed on 72 hours after PMSG injection. Calves in group 2 and 4 was received injection of 500 mg of bST every 10 days. At each TVR session, follicle number and size were recorded; the oocytes collected and graded according to cumulus and cytoplasm investment. Collected oocyte were determined viable oocyte according to morphological quality with granulation of oocyte and number and status of cumlulus cells. IVM and IVF were performed and assessed cleavage rate on day 3 after fertilization. A Sonovet 600(Medison, Co., Ltd) realtime ultrasound scanner with a 6.5 MHz convex transducer, fixed at the tip of 500 mm estended handle equipped with a needle guide was used in collecting oocyte. Differences between groups were analysed by chi-square test. The population of large follicle ($\geq$5 nun) and aspiration rate were not significant different among the 4 groups. But, the number of small follicles (<5 mm) and aspirated oocyte in the KNC calves treated with bST were 1.3~1.6 times higher than in KNC calves treat with FSH or PMSG alone. In conclusion, the administration of bST with FSH or PMSG at superovulation for TVR in calves was increase the nurnber of small follicle which was influenced the number of aspiratable follicle.

• 한국수정란이식학회지
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• 제12권2호
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• pp.195-202
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• 1997

The present study was carried out to evaluate the effect of superovulation treatments on ovarian responses, oocyte recovery rates and grades of collected oocytes using an ultrasound-guided transvaginal approach in Korean native cows. Superovulation in cows was induced with two different regimenes: 1) FSH-decreasing dose(n=8): the cows were received twice per day for three days of the total dose of 400 mg of FSH-p, 2) FSH-single dose(n=9): the cows were administrated a single dose of 400 mg of FSH-p in 25% PVP. The Observation of visible follicles and collection of oocytes were performed 12 hours following the last FSH in FSH-decreasing dose group and 48 hours after the FSH-single dose injection. All visible follicles larger than 6 mm were punctured and aspirated with a 6.5 MHz convex-array ultrasound transducer designed for intravaginal use. The mean number of visible follicles(> 6 mm) was significantly(P<0.05) higher in the FSH-decreasing dose treatment (22.811.9) and FSH-single dose treatment (20.612.0) groups than the non-treatment group(7.0$\pm$8). The mean recovery rate of oocytes was not significantly(P<0.05) different between the treatment and control groups, but the mean number of collected oocytes was significantly(P<0.05) higher in the FSH-decreasing dose treatment( 12.611.5) and FSH-single dose treatment (11.813.6) groups than the non-treatment group(3.7$\pm$0.5). In conclusion, the FSH-single dose treatment at superovulation in cows for ultrasound-guided aspiration might increase the number of aspiratable follicles and the recovery rate of follicular oocytes as the FSH-decreasing dose treatment.

• Clinical and Experimental Reproductive Medicine
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• 제17권2호
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• pp.115-121
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• 1990

Ultrasonically guided oocyte collection gradually replaces laparoscope in many IVF center. In present study, we compare the efficacy of both methods in our IVF program. Totally 377 cycles which were undertaken in vitro fertilization treatment were divided into 2 groups. Ultrasonically guided transvaginal follicular aspiration was performed in 188 cycles and laparoscopic follicular aspiration was performed in 189 cycles under local anesthesisa. The mean age for both groups was similar. Follicular recruitment was achieved with human menopausal gonadotropin (hMG) or a com bination of clomiphene citrate and hMG or a combination of FSH and hMG. In the ultrasonically guided aspiration group, 1821 follicles were aspirated with 61.8% of recovery rate (1125 oocytes), 81.5% of embryo transfer rate (145 cycles) and (17%), 26 cases intrauterine pregnancies were estabilished. In the laparoscopic group, 604 follicles were aspirated with 68.7% recovery rate (445 oocytes) and a 79.9% ET rate (127 cycles), 11 cases (8.7%) intrauterine pregnancies were estabilished. A valid comparison of these data is not possible because the 2 groups are dissimilar for factors known to influence oocyte development and recovery. No statistically significant differences could be demonstrated between 2 groups in all but the recovery rate and clinical pregnancy rate, In ultrasound group, the clinical pregnancy rate was significantly higher than that of laparoscope group. The potentially detrimental effect of CO2 pnemoperitonium present during laparoscope but not in ultrasound guided recovery on ova quality may underlie the observed difference in the clinical pregnancy rate between the 2 groups. Ultrasound guided aspiration seems to be as effective as laparoscopy in terms of oocyte retrieval and conception rate. Furthermore, the procedure is simple and inexpensive, it may replace laparoscopy as a method for oocyte collection in most patients who undergo IVF.

• 한국가축번식학회지
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• 제24권1호
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• pp.83-88
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• 2000

본 연구는 한우에 있어서 초음파기기를 이용하여 반복적으로 생체내 난자를 회수시 난자회수에 미치는 영향과 시술후의 난소 유착과 같은 부작용을 조사하기 위하여 실시한 결과는 다음과 같다. 1. 난포란의 채란빈도에 따른 채란수에서는 주 2회 시술시 난포수는 8.7$\pm$4.2개로 주 1회 시술의 10.2$\pm$6.1개보다 다소 적었으나 유의적인 차이는 인정되지 않았으며, 채란수도 각각 4.1$\pm$3.4개 및 4.3$\pm$2.9개로 유의적인 차이가 인정이 되지 않았다(P＜0.05). 2. 반복적으로 9회 난포란을 채란한 경우 40개의 난소중 8개 (20%)가 유착이 발생하였으며 1~3 채란시 3개 (37.5%), 4~6 채란시 4개 (50%) 7~9회 채란시 1개(12.5%)가 유착이 발생하였다. 3. 반복적인 난포란 채란후 난소의 유착 여부에 따른 공란우의 발정주기를 조사한 결과 난소 유착을 보인 공란우의 8두중 7두(87.5%) 25일 이상의 장발정주기를 보였으며, 유착이 일어나지 않은 12두 중 정상 발정주기는 6두(50.0%), 단 발정주기는 1두(8.3%), 장발정주기는 5두(41.7%)였다.