• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.92-103
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• 2021

Lactobacillus casei, one of the most widely used probiotics, has been reported to alleviate multiple diseases. However, the effects of this species on intestinal diseases are strain-specific. Here, we aimed to screen L. casei strains with inflammatory bowel disease (IBD)-alleviating effects based on in vitro physiological characteristics. Therefore, the physiological characteristics of 29 L. casei strains were determined, including gastrointestinal transit tolerance, oligosaccharide fermentation, HT-29 cell adhesion, generation time, exopolysaccharide production, acetic acid production, and conjugated linoleic acid synthesis. The effects of five candidate strains on mice with induced colitis were also evaluated. The results showed that among all tested L. casei strains, only Lactobacillus casei M2S01 effectively relieved colitis. This strain recovered body weight, restored disease activity index score, and promoted anti-inflammatory cytokine expression. Gut microbiota sequencing showed that L. casei M2S01 restored a healthy gut microbiome composition. The western blotting showed that the alleviating effects of L. casei M2S01 on IBD were related to the inhibition of the NF-κB pathway. A good gastrointestinal tolerance ability may be one of the prerequisites for the IBD-alleviating effects of L. casei. Our results verified the efficacy of L. casei in alleviating IBD and lay the foundation for the rapid screening of L. casei strain with IBD-alleviating effects.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.265-270
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• 2022

The purpose of this study is to derive the damage weak part of the elastic rail fastening system for concrete tracks (System 300-1). In the concrete tracks, the elastic rail fastening system sticks the rail and the sleeper and reacts all the time when the train is running. Among the components of the rail fastening system, the resilience pad and tension clamp were fatigue members and were constantly deformed in response to compressive and uplift forces. In this study, the residual deformation characteristics of the tension clamp according to the period of use were analyzed using by specimens taken on site in the same section for 6, 11, and 16 years on the serviced urban transit. In addition, the damage mechanisms for each component were derived based on finite element analysis. As a result of the numerical analysis, the stress (strain) of each part of the tension clamp according to the external force from the applied clamping force was analyzed to derive the damaged weak part of the tension clamp.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.5D
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• pp.587-595
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• 2008

Many studies on bus scheduling optimization have been done from the 1960s to recent years for establishing rational bus schedule plan that can improve convenience of bus passengers and minimize unnecessary runs. After 2000, as part of the Intelligent Transport Systems (ITS), the importance of the schedule management and providing schedule information through bus schedule optimization has become a big issue, and much research is being done to develop optimization models that will increase bus passenger convenience and, on the side of bus management, minimize unnecessary bus operation. The purpose of this study is to calculate the optimal bus frequency and create a timetable for each bus stop by applying DTR or DTRC model that use data for each bus stop and route segment. Model verification process was implemented using data collected from bus management system (BMS) and integrated transit-fare card system for bus route of Seoul's No. 472 line. In order to evaluate the reliability and uncertainty of optimal solution, sensitivity analysis was implemented for the various parameters and assumptions used in the bus scheduling model.

• Journal of Animal Science and Technology
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• v.65 no.5
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• pp.1024-1039
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• 2023

In this study, we performed a quantitative microbial risk assessment (QMRA) of Salmonella through intake of egg consumption after cooking (dry-heat, moist-heat, and raw consumption). Egg samples (n = 201) from retail markets were analyzed for the presence of Salmonella. In addition, temperature and time were investigated during egg transit, storage, and display. A predictive model was developed to characterize the kinetic behavior of Salmonella in eggs, and data on egg consumption and frequency were collected. Eventually, the data was simulated to estimate egg-related foodborne illnesses. Salmonella was not found in any of the 201 egg samples. Thus, the estimated initial contamination level was -4.0 Log CFU/g. With R² values of 0.898 and 0.922, the constructed predictive models were adequate for describing the fate of Salmonella in eggs throughout distribution and storage. Eggs were consumed raw (1.5%, 39.2 g), dry-heated (57.5%, 43.0 g), and moist-heated (41%, 36.1 g). The probability of foodborne Salmonella illness from the consumption of cooked eggs was evaluated to be 6.8×10^-10. Additionally, the probability of foodborne illness not applied cooking methods was 1.9×10^-7, indicating that Salmonella can be reduced by cooking. Therefore, the risk of Salmonella infection through consumption of eggs after cooking might be low in S. Korea.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.12
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• pp.2917-2923
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• 2015

In spite of a recent trend of container ships becoming larger in size, the current circumstance is that managing reefer containers marine transportation is being mostly dependent upon manpower. Particularly, in the case of bad weather or nighttime, reefer containers are not being monitored due to lack of safety device. For the purpose of reducing such risk, IMO is recommending a system using PLC but the system is not being used. In addition, they are still relying on manpower for control and reliability of freight in transit is decreasing due to lack of information during marine transportation for every subject related to freight as well as shipper. Accordingly, the purpose of this paper is to propose a real-time reefer container management system to effectively control all reefer containers widely being used across the world.

• Journal of Animal Science and Technology
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• v.56 no.3
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• pp.8.1-8.6
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• 2014

Background: Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barrier through laparoscope aided intrauterine AI. Therefore, AI with frozen-thawed semen is not as widespread in sheep as it is in other domestic species. However, to get maximum benefits through the use of AI, frozen-thawed semen is a prerequisite because instead of high fertility, the short shelf life of fresh semen coupled with a limitation on the number of insemination doses achievable per unit time restricts the widespread use of individual sires. Therefore, in order to enhance lambing rate, a total of 240 trans-cervical artificial inseminations with frozen-thawed semen were performed in Bharat Merino ewes during autumn season either once in the evening (G-I, 10 h after onset of estrus, n = 100) or twice (G-II, 14 h and 22 h after onset of estrus, n = 140) i.e. once in the morning and again in the evening. Results: The pregnancy rate (proportion of pregnant ewes confirmed by ultrasonography at day 40) and lambing rate (proportion of ewes lambed) were higher in G-II as compared to G-I (26.4 vs 20% and 19.3 vs 10%, respectively). The difference in lambing rates was statistically (P < 0.05) significant. The depth of insemination within cervico-uterine tract had no significant effect on pregnancy and lambing rates. Conclusions: The results indicate that lambing rate in sheep following TCAI with frozen-thawed semen was significantly influenced by time of inseminations. Two inseminations after 14 and 22 h of onset of estrus enhanced the lambing rates of Bharat Merino sheep as compare to single insemination after 10 h of onset of estrus. The TCAI technique with frozen-thawed ram semen is promising and may serve as a valuable tool for genetic improvement of sheep breeds. Research efforts are going on worldwide to overcome the poor fertility following TCAI with frozen-thawed semen.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.618-624
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• 2017

In Seoul and most metropolitan cities, urban trains are delayed due to high congestion during commute times. The delay effect of passengers boarding and disembarking is also significant. In this study, a wide passenger door system was developed as a way to improve the scheduled speed of urban trains by decreasing the passengers' flow time. The door size was defined experimentally to shorten the entrance time. The optimum door size was also determined to improve the stop precision performance of the train while considering the interference effect with peripheral devices. Because the change in door size changes the structural characteristics of the vehicle, the structural stability of a train was analyzed numerically. A prototype of the wide door system was made, and the proposed design was verified using functional and endurance tests. The systematic development process can be used as design data for door size definition and system production when applying a wide door to improve the scheduled speed.

• Journal of the Korean Society for Railway
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• v.12 no.4
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• pp.582-589
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• 2009

The energy generated by braking vehicle would simply be converted into waste heat by its braking resistors if no other vehicle is accelerating at exactly the same time. Up to 45% of the tractive power of vehicles capable of returning energy to the power supply can be regenerated during braking and that this energy can be used to feed vehicles which are accelerating at the same time. Such synchronized braking and accelerating can not be coordinated, the ESS(energy storage system, here after) stores the energy generated during braking and discharges it again when a vehicle accelerates. The ESS is able to store and discharge energy extremely quickly, consequently enabling a complete exchange of energy between vehicles, even if they are not braking and accelerating at precisely the same time, as is most frequently the case in everyday service. The energy saving rate is related to the headway. If the headway is long/short, the energy saving goes up/down, When the headway is short, the ESS can not save much regenerative energy. The headway of SeoulMetro line 2 as the worst case is very short in Korea urban transit system. So, the energy saving rate will be very low. If the ESSs are applied to another railway system, we can expect that the effectiveness is better than the results of SeoulMetro line 2. This paper presents effects of energy saving obtained by applying the ESS to SeoulMetro line 2.

• Journal of Chest Surgery
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• v.36 no.9
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• pp.659-665
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• 2003

The aim of this study was to evaluate the early and midterm results of off-pump total arterial revascularization using the skeletonized right gastroepiploic artery (RGEA) as a third arterial conduit. Material and Method: We prospectively analyzed 103 patients who underwent off-pump total arterial revascularization using bilateral internal thoracic arteries (ITAS) and RGEA. The RGEA was used as in situ graft in 88 patients, composite graft in 10 patients, and free graft in 5 patients. Postoperative coronary angiographies were performed before discharge in 100 patients, and at postoperative one year in 88 patients. Result: The RGEA showed a significantly higher free flow (130$\pm$95 ml/min) than that of right ITA(113$\pm$57 ml/min) or left ITA (107$\pm$55 ml/min), which was measured before anastomosis (p < 0.05). The total number of distal anastomoses was 3.8$\pm$0.7. The number of distal anastomoses per bilateral ITAs was 2.8$\pm$0.7 and the number of distal anastomosis per RGEA was 1.0. There were two morialities including one operative mortality. The late mortality was not related to cardiac events. Early postoperative morbidities were atrial fibrillation in 15 patients, bleeding reoperation in 4 patients, mediastinitis in 1 patients, perioperative myocardial infarction in 2 patient, and transient ARF in 3 patients. Postoperative coronary angiographies showed the early patency rate of 98.6％ (272/276) for ITAs and 97.0％ (97/100) for RGEA, respectively (p=ns), and the one-year patency rate of 95.9％ (234/244) for ITAs and 88.6％ (78/88) for RGEA, respectively (p=0.07). Flow competition between the RGEA and NCA (native coronary artery) was seen in 5 of the 100 patients (5.0％) immediate postoperatively and 7 of the 88 patients (8.0％) 1 year after surgery. Since July, 2000, we measured transit time flow intraoperatively and could reduce flow competition significantly Conclusion: The skeletonized RGEA demonstrated excellent early and midterm patency rates and could be used as a third arterial graft following the bilateral ITAs.

• Journal of Chest Surgery
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• v.30 no.8
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• pp.760-769
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• 1997

This study aimed to determine factors that influence blood flow through coronary bypass grafts and to analyze relationship between the graft flow and postoperative outcome. Blood flow through 146 bypass grafts(GBF) was measured with transit-time ultrasound flowmeter during coronary artery bypass grafting operations in 50 patients. Single and multiple regression analyses were done for relationships between the GBF and four variables: internal diameter of recipient coronary artery, myocardial value of bypassed branch(es), type of graft, and finding of preoperative myocardial perfusion scan. The relationship between GBF and postoperative scan finding was also analyzed. 1. The mean GBF was significantly higher in sequential grafts than in single vein grafts or in internal thoracic artery grafts(61.5 vs. 46.9 and 42.5 ml/min). 2. Myocardial value and recipient artery diameter were found to be the factors determining GBF. There was no correlation between GHF and presence of perfusion defect in the preoperative scan. 3. Myocardial value was found to be more important than recipient artery diameter in determinintg GBF. 4. Reversible perfusion defects were more frequently found in the areas upplied by grafts with low GBP. But this fact had only mild statistical significance. These results suggest that blood flow through a bypass graft is more determined by the size of its supplyinf: myocardium than by the size of recipient artery. So, we can expect effective improvement in myocardial flow reserve after grafting of small(1~1.5mm) coronary arteries, if they supply substantial area of myocardium.