• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.257.2-257
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• 1997

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.484-486
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• 2006

Maltosyl (G2)-mannitol, produced by the transglycosylation of mannitol with maltotriose by Bacillus stearothermophilus maltogenic amylase, was not found to support lactic acid production by Streptococcus sobrinus NRRL 14555. Furthermore, the synthesis of water-insoluble glucans from maltosyl-mannitol by S. sobrinus NRRL 14555 was much lower than that from xylitol or mannitol. Consequently, these results suggest that maltosyl-mannitol could be used as a noncariogenic sugar substitute in food products.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.219-222
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• 1999

A simple sequence of membrane concentration and DEAE-Cellulose chromatography has been optimized to give a purified dextransucrase from Leuconostoc mesenteroides B-512FMCM with the highest specific activity (248.8 IU/mg protein) ever reported in high yield (overall 88.7%) for dextransucrase. When there was no sucrose in the dextransucrase and the dextran reaction digest, the dextransucrase hydrolyzed glucose from dextran. The glucose was transferred to the other glucoses from dextran and formed isomaltose and isomaltodextrin. The transglycosylation efficiency of glucose from dextran was much higher with acceptors. The dextransucrase can be used for the production of various kinds (or structures) of oligosaccharides using dextran and various acceptors with almost 100% theoretical yield.

• 한국연초학회지
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• 제24권2호
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• pp.94-100
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• 2002

Cyclodextrin glucanotransferase from Bacillus stearothemophilus and Bacillus macerans synthesized maltol and ethyl maltol monoglucoside, with a series of its maltooligo-glucosides by transglycosylation with dextrin as a donor, and maltol or ethyl maltol as an acceptor. The monoglucoside formed from reaction mixture of maltol or ethyl maltol by the successive actions of Bacillus stearothemophilus cyclodextrin glucanotransferase and Rhizopus glucoamylase was isolated by Diaion HP-20 column and silica gel column chromatography. The structure of the isolated monoglucoside was identified as maltol-$\alpha$-D-glucoside and ethyl maltol-$\alpha$-D-glucoside, respectively, by FAB-MS, UV, $^1$H-NMR, $^{13}$ C-NMR spectra and products by hydrolysis with acid, $\alpha$ - and $\beta$ -glucosidases.

• 미생물학회지
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• 제25권4호
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• pp.304-308
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• 1987

The mode of transglycosylation reaction observed during the action of low-molecular-weight 1,4-$\beta$-D-glucan glucanohydrolase (EC 3.2.1.4) purified from Trichoderma koningii ATCC 26113 was investigated using $^{1}H-NMR $spectroscopy. The H-1 proton resonances were analysed. After reaction of the enzyme with cellotriose, the reaction products were separated by high performance liquid chromatography. H-1 resonances of the products were consisted with those of cellobiose, cellotriose and cellotitraose, respectively. Therefore it was proved that all the reaction products formed by the action of the enzyme on cellooligosaccharides, including transglycosylation products, possess only H-NMR -1,4-glycosidic linkage(s).

• Food Science and Biotechnology
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• 제17권2호
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• pp.302-307
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• 2008

Novel amylase inhibitors were synthesized via transglycosylation by Thermotoga maritima glucosidase (TMG). TMG hydrolyzes acarbose, acarviosine-glucose, and maltooligosaccharide by releasing $^{14}C$-labeled glucose from the reducing end of each molecule. When TMG was incubated with acarviosine-glucose (the donor) and glucose (the acceptor), two major transfer products, compounds 1 and 2, were formed via transglycosylation. The structures of the transfer products were determined using thin-layer chromatography (TLC), high-performance ion chromatography (HPIC), and $^{13}C$ nuclear magnetic resonance (NMR) spectroscopy. The results indicate that acarviosine was transferred to glucose at either C-6, to give a $\alpha-(1{\rightarrow}6$) glycosidic linkage, or at C-3, to produce an $\alpha-(1{\rightarrow}3$) glycosidic linkage. The transfer products showed a mixed-type inhibition against porcine pancreatic $\alpha$-amylase; therefore, they may be useful not only as inhibitors but also as acarbose transition-state analogs to study the mechanism of amylase inhibition.

• 한국식품영양과학회지
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• 제41권10호
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• pp.1417-1422
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• 2012

Alkyl-glucoside의 생산을 위하여 상용화 cellulase인 Celluclast의 당전이 반응을 사용하였다. 5가지 종류의 알코올을 acceptor molecule로 하여 반응을 살펴본 결과 methyl alcohol, ethyl alcohol, isopropanol 그리고 butanol에서 당전이 반응이 일어남을 확인하였다. 반응 수율이 높았던, methyl alcohol과 ethyl alcohol의 반응산물을 MALDI-TOF MS와 효소적인 방법을 사용하여 각각의 산물이 methyl ${\beta}$-D-glucopyranoside와 ethyl ${\beta}$-D-glucopyranoside임을 확인하였다. 시간대별 methyl-glucoside와 ethyl-glucoside의 생산량을 비교하여 본 결과 9시간에서 최대 생산 수율 65% (mol/mol)와 59%(mol/mol)를 각각 보였으며, 이후 반응은 진행되지 않았다. Cellulose의 당전이 반응으로 생성된 부산물인 glucose를 제거하기 위하여 고정화 효모 system을 도입하였고, 그 결과 glucose를 모두 제거할 수 있었다. 이상의 결과에서 Celluclast를 이용한 alkyl-glucoside의 생산을 성공적으로 수행하였고, 고정화 효모 system을 도입하여 친환경적으로 부산물을 제거하여 고순도의 ethyl-glucoside를 생산하였다.

• 생명과학회지
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• 제21권11호
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• pp.1631-1635
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• 2011

재조합 아밀로수크라아제의 폴리페놀 배당체를 합성하는 능력을 검사하였다. 이 효소의 효소작용 특성에 근거하여 설탕을 기질로 사용하였으며 21 종류의 각기 다른 폴리페놀 화합물들이 수용체로 사용되었다. 당 전이 반응은 사용한 폴리페놀에 따라 하나 또는 두 개의 주요 폴리페놀 배당체를 합성하였다. 합성된 폴리페놀 배당체들은 박막 크로마토그래피법을 이용하여 확인되었고, 새로이 합성된 배당체의 구조는 당 전이 작용 특성에 근거하여 예측되었다. 수용체로 가능한 폴리페놀의 구조적 특징들이 평가되었으며, 이러한 결과는 Deinococcus geothermalis 유래 아밀로수크라아제가 식품, 화장품, 및 제약산업에서 높은 잠재성을 갖는 폴리페놀 배당체의 효소적 합성에 매우 효율적인 촉매로 활용될 수 있다는 것을 보여준다.

• KSBB Journal
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• 제15권1호
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• pp.35-41
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• 2000

Xylitol을 당수용체로하여 당알콜 올리고당 gluosyl-xylitol을 생산하기 위하여 갭술고정화 전세포 CGTase를 제조하고자 하였다. CGTase를 생산하기 위하여 Bacillus macerans를 배양하는 경우 organic form의 질소원을 사용하는 경우 inorganic form의 질소원을 사용하는 경우보다 더 많은 CGTase를 생산하였고 배양도중 탄소원인 starch가 분해되는 동안 CGTase가 생성되었다. B. macerans에 의하여 생산된 CGTase는 80% 이상이 extracellular cnzyme 이며, intracellular enzyme은 20% 이내이었다. E. coh, C. glutamicum, S. cerevisiae 등과 달리 캡슐내부에 B. macerans를 접종하고 캡슐내부에서 고농도로 배양할 수 없었다. 배양액속에 존재하는 CGTase는 다른 이온성 물질들로 인하여 활성탄, Ambolite, Sephadex 등의 흡착제에 흡착시킬 수 없었다. 미생물을 배양한 배양책 전체를 10배로 농축하여 캡슐내에 고정화함으로써 캡슐고정화 전세포 CGTase를 제조할 수 있었다. 농축배양액을 이요하여 제조된 캡슐고정화 전세포 CGTase는 hydrolysis, intermolecular transglycosylation을 수행하였으며, xylitol을 당수용체로 하고 dextrin을 당공여체ㅗ 하여 glucosyl-xylitol을 생산하였다.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.579-587
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• 2018

For biotechnological production of high-valued ${\beta}-{\text\tiny{D}}$-hexyl glucoside, the catalytic properties of Hanseniaspora thailandica BC9 ${\beta}$-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside was optimized. The constitutive BC9 ${\beta}$-glucosidase exhibited maximum specific activity at pH 6.0 and $40^{\circ}C$, and the activity of BC9 ${\beta}$-glucosidase was not significantly inhibited by various metal ions. BC9 ${\beta}$-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 ${\beta}$-glucosidase exhibited enhanced production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside in the presence of DMSO, and 62% of ${\beta}-{\text\tiny{D}}$-hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.