Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

$^{1}H-NMR$ Spectroscopic Evidence on the Glycosidic Linkages of the Transglycosylated Products of Low-Molecular-weight 1,4-$\beta$-D-Glucan Glucanohydrolase from Trichoderma koningii

Trichoderma koningii에서 분리한 저분자 1, 4-$\beta$-D-Glucan Glucanohydrolase의 반응산물에 대한 핵자기공명 스펙트럼 분석

  • 맹필재 (서울대학교 자연과학대학 미생물학과) ;
  • 강사욱 (서울대학교 자연과학대학 미생물학과) ;
  • 정춘수 (서울대학교 자연과학대학 미생물학과) ;
  • 홍순우 (서울대학교 자연과학대학 미생물학과) ;
  • 하영칠 (서울대학교 자연과학대학 미생물학과) ;
  • 이영하 (충남대학교 자연과학대학 미생물학과) ;
  • 김재헌 (단국대학교 이공대학 미생물학과)
  • Published : 1987.12.01
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Abstract

The mode of transglycosylation reaction observed during the action of low-molecular-weight 1,4-$\beta$-D-glucan glucanohydrolase (EC 3.2.1.4) purified from Trichoderma koningii ATCC 26113 was investigated using $^{1}H-NMR $spectroscopy. The H-1 proton resonances were analysed. After reaction of the enzyme with cellotriose, the reaction products were separated by high performance liquid chromatography. H-1 resonances of the products were consisted with those of cellobiose, cellotriose and cellotitraose, respectively. Therefore it was proved that all the reaction products formed by the action of the enzyme on cellooligosaccharides, including transglycosylation products, possess only H-NMR -1,4-glycosidic linkage(s).

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