• Korean Journal of Microbiology
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• v.52 no.3
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• pp.249-253
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• 2016

The inky cap, Coprinellus congregatus, produces several laccase isozymes during its life cycle: both hyphal tip laccase and sclerotial laccase are involved in the fungal development. When this fungus was transferred to an acid liquid medium (pH 4.0-4.5), a new laccase was synthesized and secreted into the culture supernatant. In order to examine its regulation by external pH, green fluorescent protein gene was ligated at the downstream of the promoters having different lengths. These expression vectors having different promoter lengths were inserted into the fungal transformation vector, pBARGEM7-1. These expression vectors were introduced to the mating type a1 and a2 monokaryons, and the transformants were selected by the phosphinothricin resistance. Transformant a1 (a1TF) and transformant a2 (a2TF) were mated with each other to generate homozygotic dikaryon transformants. All these transformants were grown in neutral liquid medium for 5 days, and then the whole cell homogenates were transferred to the acidic liquid medium (pH 4.1). After 36 h incubation at $25^{\circ}C$, cells were harvested for the analysis of GFP expression. GFP expression was detected in the transformant having full-length promoter (2.0 kb), but other transformants having shorter length promoter (shorter than 1.29 kb) failed to show the fluorescence. Therefore, the acid-responsive element in the laccase promoter should be localized between -2.0 kb ~ -1.29 kb region.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.7
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• pp.565-572
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• 2009

Greenhouse gas(GHG) inventories and basic strategies for Jeonbuk regional government were established to reduce greenhouse gas emissions. The method to construct GHG inventories of Jeonbuk followed the 'Revised IPCC 1996 Guidelines'which was used for the 'Third National Communication of the Republic of Korea under UNFCCC'. Korean government could use primary energy consumption for the energy industries section in the national GHG inventories. However, regional governments should use secondary energy consumption (included electricity consumption) for the energy industries section for their GHG inventories because they could not control the emission of energy transformation section. In the result of Jeonbuk GHG inventories in 2006, carbon dioxide($CO_2$) emissions from fuel combustion covered 87.1% of total emissions. Methane($CH_4$), carbon dioxide($CO_2$) from other sections, nitrous oxide($N_2O$) and F-gas(HFCs, PFCs, $SF_6$) accounted for 8.1, 2.2, 1.6 and 1.0% of total emissions, respectively. The sectional emission decreased in the order of the energy(88.0%), agriculture(7.6%), waste(2.3%) and industrial processes(2.1%) section. The energy industries section that contained electricity consumption was the most dominant emission source in the energy section. F-gas consumption, rice cultivation and waste incineration were main emission sources in the industrial processes, agriculture and waste section, respectively. In this study, basic directions of each section were established by the results of Jeonbuk GHG inventories in 2006.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.343-353
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• 2021

Historical dramas are changing in response to the public who wants more dramatic development, and historical costumes are also expressed in a modern sense. The purpose of this study is to examine the modernization of historical costumes in historical dramas and how the modernization of historical costumes in historical dramas is fostered from the learner's point of view with suggesting implications. As a result of this study, first, the essential problem with the modernization of historical costumes was the excessive modern transformation that undermines historical facts in historical dramas. Second, the negative perceptions of the modernization of historical costumes in historical dramas included the loss of the unique Korean identity, decreased immersion in drama, and the educational influence of media. Positive perceptions focused on the increased interest through raising awareness of traditional culture, compromises on changes in the times, and increased visual play of the public. Third, the implications of the modernization of historical costumes in historical dramas require the awareness improvement of participants in historical drama and a thorough preliminary investigation by the costume designer on the historical costume, an in-depth study of traditional costumes, a systematic educational approach, viewers' attention, and government effort.

• Journal of the Korean Society for information Management
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• v.39 no.3
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• pp.165-193
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• 2022

It can be said that digital technology is at the center of the change in the modern work environment. In particular, in general public institutions that prove their work with records produced by business management systems and document production systems, the record management system is also the work environment itself. Gimpo City applied for the 2021 public cloud leading project of the National Information Society Agency (NIA) to proactively respond to the 4th industrial revolution technology era and implemented a public cloud-based AI-OCR technology enhancement project with 330 million won in support of 330 million won. Through this, it was converted into data beyond the limitations of non-electronic records limited to search and image viewing that depend on standardized index values. In addition, a 98% recognition rate was realized by applying a new technology called AI-OCR. Since digital technology has been used to improve work efficiency, productivity, development cost, and record management service levels of internal and external users, we would like to share the direction of enhancing expertise in the record management and implementation of work environment innovation.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.37-44
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• 2005

Microspores were isolated from pepper (Capsicum annuum L.) anthers by using a micro-blender and cultured in modified NLN medium at $25^{\circ}C$. The influence of pretreatment period at $32^{\circ}C$, adding the 2-hydroxynicotinic acid to a pretreatment medium, and co-pretreatment anthers with microscopes on the induction of embryo were examined. Globular and torpedo embryos were observed from 3 weeks after culture. Embryo development was not synchronized within culture. After 4 weeks in culture, in addition to globular and torpedo embryos, cotyledonary embryos were observed. Normal cotylodonary embryos developed into plantlets when transferred to a solid hormone free B5 medium containing $2\%$ sucrose. Embryo yields were significantly higher after 1- and 2-day pretreatment at $32^{\circ}C$. However the development of embryo ceased at the globular or heart stage. In contrast, embryo yields were lower after 3- to 6-day pretreatment at $32^{\circ}C$ and embryo developed at the cotyledonary stage. After adding the 2-hydroxynicotinic acid to anther pretreatment solution, embryo yields were slightly increased. However most embryos occurred were at the globular or heart stage. Co-pretreatment of microspores with anthers was deleterious for embryo induction and development. AS far as we know, this is the first report of success in obtaining high frequency of embryogenesis and plantlets formation from isolated microspores of pepper. Although the culture conditions have to be optimized further, this promising microspore culture system can be used for genetic transformation, selection for dominant and recessive traits as well as for the production of homozygous doubled haploid plants.

• The Journal of Society for e-Business Studies
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• v.11 no.3
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• pp.53-71
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• 2006

In order to cope with the rapidly changing product development environment, individual manufacturing enterprises are forced to collaborate with each other through establishing a virtual organization. In collaboration, designated organizations work together for mutual gain based on product data interoperability. However, product data interoperability is not fully facilitated due to semantic inconsistency among product data models of individual enterprises. In order to overcome the semantic inconsistency problem, this paper proposes a reference ontology, Reference Domain Ontology(RDO), and a methodology for product data interoperability with semantic consistency using RDO. RDO describes semantics of product data model and metamodel for all application domains in a virtual organization. Using RDO, application domains in a virtual organization can easily understand the product data models of others. RDO is agile and temporal such that it is created with the formation of a virtual organization, copes with changes of the organization, and disappears with the vanishment of the organization. RDO is built by a hybrid approach of top-down using a upper ontology and bottom-up based on the merging of ontologies of application domains in a virtual organization. With this methodology, every domain in a virtual organization can achieve product data model interoperability without model transformation.

• Journal of Life Science
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• v.13 no.5
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• pp.551-558
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• 2003

Hepatitis C Virus (HCV) is associated with a severe liver disease and increased frequency in the development of hepatocellular carcinoma. Overexpression of HCV core protein is known to transform fibroblast cells. Phospholipase D (PLD) activity is commonly elevated in response to mitogenic signals, and PLD has been also reported to be overexpressed and hyperactivated in some human cancer. The aim of this study was to understand how PLD can be regulated in HCV core protein-transformed NIH3T3 mouse fibroblast cells. We observed that in unstimulated state, basal PLD activity was higher in NIH3T3 cells overexpressing HCV core protein than in vector-transfected cells. Although expression of PLD and protein kinase C (PKC) in core protein-transformed cells was similar with that of control cells, phorbol 12-myristate 13-acetate (PMA), which is known to activate PKC, stimulated significantly PLD activity in core protein-transformed cells, compared with that of the control cells. PLD activity assay using PKC isozyme-specific inhibitor, and PKC translocation experiment showed that PKC-$\delta$ was mainly involved in the PMA-induced PLD activation in the core-transformed cells. Taken together, these results suggest that PLD might be implicated in core protein-induced transformation.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.121-126
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• 2012

To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.281-286
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• 2005

To develop transgenic birdsfoot trefoil (Lotus corniculatus L.) plants tolerant to environmental stress, Arabidopsis NDPK gene (AtNDPK) was introduced into birdsfoot trefoil plants using Agrobacterium-mediated transformation and expressed powerfully under the control of the SWPA2 promoter. The expression vector, pCAMBIA2300 was used for introduction of AtNDPK gene into birdsfoot trefoil plants. The transformed calli were selected on kanamycin containing medium and then regenerated. The transformed birdsfoot trefoil plants were cultivated fur 4 months on BOi2Y medium. Genomic DNA PCR and Southern blot analysis confirmed the incorporation of AtNDPK into the birdsfoot trefoil genome.

• Journal of Broadcast Engineering
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• v.17 no.2
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• pp.305-315
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• 2012

Color correction between two or multiple images is very crucial for the development of subsequent algorithms and stereoscopic 3D camera system. Even though various color correction methods are proposed recently, there are few methods for measuring the performance of these methods. In addition, when two images have view variation by camera positions, previous methods for the performance measurement may not be appropriate. In this paper, we propose a method of measuring color difference between corresponding images for color correction. This method finds matching points that have the same colors between two scenes to consider the view variation by correspondence searches. Then, we calculate statistics from neighbor regions of these matching points to measure color difference. From this approach, we can consider misalignment of corresponding points contrary to conventional geometric transformation by a single homography. To handle the case that matching points cannot cover the whole regions, we calculate statistics of color difference from the whole image regions. Finally, the color difference is computed by the weighted summation between correspondence based and the whole region based approaches. This weight is determined by calculating the ratio of occupying regions by correspondence based color comparison.